• KSBB Journal
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• 제27권1호
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• pp.1-8
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• 2012

Recently, translational research (TR) in health technology (HT) has been considered as an emerging alternative research system for the improvement of human health. TR from bench to bedside involves a strong bidirectional relationship between basic science discovery and clinical practice. To support R&D planning and policy in HT effectively, the performance of TR programs was analyzed and evaluated in a R&D project on health and medical technology. TR programs were classified into three parts: unilateral TR, bilateral TR and multilateral TR. Bibliometrics and citation analysis were performed to assess research papers and gather information for the performance analysis of TR programs. In addition, both quantitative and qualitative analysis were successfully carried out using ISI Web of Science, Google Scholar Citations, SCOPUS and Knowledgematrix. In conclusion, the performance analysis of TR programs could significantly improve the efficiency of R&D plans, R&D management and evaluation for a safe and healthy life.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 추계학술발표회
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• pp.370-371
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• 2011

• Biomolecules & Therapeutics
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• 제29권5호
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• pp.506-518
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• 2021

The imprinted tumour suppressor NOEY2 is downregulated in various cancer types, including ovarian cancers. Recent data suggest that NOEY2 plays an essential role in regulating the cell cycle, angiogenesis and autophagy in tumorigenesis. However, its detailed molecular function and mechanisms in ovarian tumours remain unclear. In this report, we initially demonstrated the inhibitory effect of NOEY2 on tumour growth by utilising a xenograft tumour model. NOEY2 attenuated the cell growth approximately fourfold and significantly reduced tumour vascularity. NOEY2 inhibited the phosphorylation of the signalling components downstream of phosphatidylinositol-3'-kinase (PI3K), including phosphoinositide-dependent protein kinase 1 (PDK-1), tuberous sclerosis complex 2 (TSC-2) and p70 ribosomal protein S6 kinase (p70S6K), during ovarian tumour progression via direct binding to vascular endothelial growth factor receptor-2 (VEGFR-2). Particularly, the N-terminal domain of NOEY2 (NOEY2-N) had a potent anti-angiogenic activity and dramatically downregulated VEGF and hypoxia-inducible factor-1α (HIF-1α), key regulators of angiogenesis. Since no X-ray or nuclear magnetic resonance structures is available for NOEY2, we constructed the three-dimensional structure of this protein via molecular modelling methods, such as homology modelling and molecular dynamic simulations. Thereby, Lys15 and Arg16 appeared as key residues in the N-terminal domain. We also found that NOEY2-N acts as a potent inhibitor of tumorigenesis and angiogenesis. These findings provide convincing evidence that NOEY2-N regulates endothelial cell function and angiogenesis by interrupting the VEGFR-2/PDK-1/GSK-3β signal transduction and thus strongly suggest that NOEY2-N might serve as a novel anti-tumour and anti-angiogenic agent against many diseases, including ovarian cancer.

• 한국응용과학기술학회지
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• 제39권3호
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• pp.442-453
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• 2022

다양한 동물 모델이 인간 질병, 의약품의 효능 및 작용 메커니즘을 연구하는 데 사용되고 있다. Zebrafish(Danio rerio)는 여러 가지 장점이 있어 인간 질병에 대한 중개 연구의 모델로 점점 더 폭넓게 활용되고 있다. 본 논문은 Pubmed, Google Scholar, Scopus에서 2020년 12월까지 최근 10년간 zebrafish 모델, 천연물(한약), in vivo 스크리닝의 키워드를 사용하여 저널에 게재된 논문을 검토하여 필요한 정보를 얻었다. 이 리뷰에서 우리는 천연물(한약) 연구에 대한 다양한 제브라피쉬 질병 모델의 최근 경향에 대해 논의하였다. 특히, 암, 안질환, 혈관 질환, 당뇨병 및 합병증, 피부질환에 중점을 두었고, zebrafish 배아를 사용하여 이들 질병에 대한 의약품의 분자 작용 메커니즘에 관해 언급하였다. Zebrafish는 실험실에서 임상 연구까지의 격차를 줄이는 데 중추적 역할을 할 수 있는 중요한 동물 모델이다. Zebrafish는 의약품이나 화장품 개발, 질병의 병인론을 이해하기 위해 사용되고, 이로 인해 생의학 연구에서 설치류의 사용을 줄이는 데 크게 기여하고 있다.

• Journal of Ginseng Research
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• 제25권4호
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• pp.156-161
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• 2001

인삼의 항암효과의 분자적 기전을 규명하기 위하여 인삼의 성분 중 강력한 항암효과를 가지는 것으로 보고된 polyacetylene 계 성분이 Ras 단백질의 post-translational modification에 관여하는 효소인 farnesyl protein trasnferase(FPTase)와 carboxyl methyl transferase(CMTase)의 활성에 미치는 영향을 확인하였고, 인삼의 지용성 성분(PEE)이 세포주기에 미치는 영향을 확인하였다. 소의 뇌로부터 FPTase와 CMTase를 부분정제하여 인삼의 polyacetylene계 성분이 두 효소의 활성에 미치는 영향을 확인한 결과, FPTase는 10mM panaxynol과 10mM panaxydol에 의해서 효소활성이 각각 16.2%와 21.3% 저해받았고, CMTase 효소활성은 polyacetylene계 성분인 panaxynol과 pnanxydol에 의해서 활성을 저해받지 않는 것으로 나타났다. 인체 결장 암세포인 HT-29와 인체 간암세포인 HepG2의 배양액에 인삼의 PEE 성분의 첨가배양시 세포크기가 상당히 위축되며 사멸되었음을 볼 수 있었고, 세포주기 분석 결과 인삼의 PEE 성분의 첨가배양시 G1 단계 세포가 증가하고, S단계세포가 감소하여 세포주기의 진행이 G1-S 단계에서 현저히 억제됨을 나타내었다. 이상의 결과로 보아 인삼의 지용성 성분에 의한 항암효과는 Ras의 post-translational modification 에 관여하는 효소의 활성저해에 기인하기보다는 세포주기의 진행 과정에서 세포주기 조절인자들의 발현변화 및 세포주기의 진행에 관여하는 단백질의 활성억제와 관련이 있을 것으로 사료된다.

• 한국전산구조공학회논문집
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• 제35권5호
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• pp.277-286
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• 2022

국내의 서비스 수준 지진(SLE)과 최대 고려 지진(MCE)의 두 RC 건물 구조물의 실험 및 해석 결과에서 얻은 탄성 및 비탄성 응답은 비틀림에 대한 전단 및 비틀림 거동에서 저항 메커니즘의 특성을 연구하는데 사용될 수 있다. 불균형 구조의 특성 연구에서는 전단력 및 비틀림 모멘트에 대한 병진 변형 및 비틀림 변형의 상호 작용 효과를 나타내는 방정식이 제안하였다. 탄성과 비탄성 거동에서 힘과 변형 사이에 상관 관계 유무가 다르기 때문에 증분 전단력과 증분 비틀림 모멘트를 최대 벽 프레임 변형을 중심으로 항복, 제하 및 재하중 단계로 구분하여 해당 증분 변형 및 증분 비틀림 변형 측면에서 해석을 수행하였다. 두 가지 주요 지배 모드의 탄성 조합에서 병진 변형은 주로 전단력에 기여하는 반면 비틀림 변형은 전체 비틀림 모멘트에 크게 기여한다. 그러나 비탄성 응답에서는 증분 병진 변형이 증분 전단력과 증분 비틀림 모멘트 모두에 기여하게 된다. 따라서 주어진 방정식을 이용하여 비탄성 응답에서 비틀림의 편심 감소, 비틀림 강성 저하 및 겉보기 에너지 생성과 같은 모든 현상들을 설명하였다.

• 대한한의학회지
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• 제35권4호
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• pp.24-35
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• 2014

Objectives: Although the autonomic nervous system (ANS) is thought to play an important role in treatment of obesity, no study has been conducted to investigate acupuncture's effects on this aspect of the ANS. This study aimed to describe the effects of acupuncture in the ANS by means of heart rate variability (HRV) analysis. Methods: A total of 46 obese women aged from 21 to 54 with body mass index ranging from 25.1 to $39.3kg/m^2$ were recruited and randomized into both the real acupuncture group (n=23) and sham acupuncture group (n=23). A total of 3 instances of HRV analysis were conducted before, during, and after treatment. Statistically significant differences between time and groups were analyzed using repeated measure analysis of variance. Results: All parameters of time domain analysis and frequency domain analysis except for the square root of the mean squared differences of successive normal sinus intervals (RMSSD) and very low frequency (VLF) showed significant differences between times. The mean of all R-R intervals (mean RR) showed significant level of interaction between time and group. Between groups, time domain analysis of standard deviation of the normal-to-normal intervals (SDNN), RMSSD and frequency domain analysis of total power (TP) and high frequency (HF) showed significant differences. Conclusions: The real acupuncture group showed deactivation of parasympathetic function and relative increase of sympathetic activity in obese subjects. Further studies are necessary to uncover the mechanisms of acupuncture in obesity treatment.

• The Korean Journal of Physiology and Pharmacology
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• 제19권3호
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• pp.257-262
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• 2015

It is well known that cigarette smoke can cause erectile dysfunction by affecting the penile vascular system. However, the exact effects of nicotine on the corpus cavernosum remains poorly understood. Nicotine has been reported to cause relaxation of the corpus cavernosum; it has also been reported to cause both contraction and relaxation. Therefore, high concentrations of nicotine were studied in strips from the rabbit corpus cavernosum to better understand its effects. The proximal penile corpus cavernosal strips from male rabbits weighing approximately 4 kg were used in organ bath studies. Nicotine in high concentrations ($10^{-5}{\sim}10^{-4}M$) produced dose-dependent contractions of the corpus cavernosal strips. The incubation with $10^{-5}M$ hexamethonium (nicotinic receptor antagonist) significantly inhibited the magnitude of the nicotine associated contractions. The nicotine-induced contractions were not only significantly inhibited by pretreatment with $10^{-5}M$ indomethacin (nonspecific cyclooxygenase inhibitor) and with $10^{-6}M$ NS-398 (selective cyclooxygenase inhibitor), but also with $10^{-6}M$ Y-27632 (Rho kinase inhibitor). Ozagrel (thromboxane $A_2$ synthase inhibitor) and SQ-29548 (highly selective TP receptor antagonist) pretreatments significantly reduced the nicotine-induced contractile amplitude of the strips. High concentrations of nicotine caused contraction of isolated rabbit corpus cavernosal strips. This contraction appeared to be mediated by activation of nicotinic receptors. Rho-kinase and cyclooxygenase pathways, especially cyclooxygenase-2 and thromboxane $A_2$, might play a pivotal role in the mechanism associated with nicotine-induced contraction of the rabbit corpus cavernosum.

• Asian Pacific Journal of Cancer Prevention
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• 제15권19호
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• pp.8047-8050
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• 2014

Cancer is a multifaceted and genomically complex disease and research over decades has gradually and sequentially shown that essential biological mechanisms including cell cycle arrest and apoptosis are deregulated. The benefits of essential oils from different plants have started to gain appreciation as evidenced by data obtained from cancer cell lines and xenografted mice. Encouraging results obtained from preclinical studies have attracted considerable attention and various phytochemicals have entered into clinical trials.

• BMB Reports
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• 제51권8호
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• pp.373-377
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• 2018

Triple-negative breast cancer (TNBC) is considered to be a notorious type of cancer due to its aggressive metastatic potential and poor prognosis. Recent evidence suggests that BLT2, a low-affinity $LTB_4$ receptor is critically associated with the phenotypes of TNBC cells, including invasion, metastasis, and survival. Furthermore, in a group of 545 breast cancer patients with metastasis, we observed that the high-BLT2 subgroup had a lower disease-free-survival rate than the low-BLT2 subgroup. Thus, we theorized that anti-BLT2 strategies could facilitate the development of new therapies used for TNBC. This review focuses on recent discoveries regarding BLT2 and its roles in as a novel prognostic biomarker in TNBC.