• International Journal of Computer Science & Network Security
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• 제22권9호
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• pp.75-82
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• 2022

Analyzing the psychological and pedagogical literature, we found the researchers' interest in the problem posed. The concept of "culture of professional communication» is considered, which is interpreted as the level of realization of creative abilities, exchange of messages, organization of mutual understanding, mutual knowledge in the process of professionally directed interaction between subjects, in which interpersonal relationships arise, manifest and form. The concept of "professional culture of communication of a teacher" is interpreted. The motives that are socially significant in the professional communication of the teacher are highlighted. The necessity of forming a culture of professional communication among students, in particular by means of information technologies in the present, is clarified. The interactive component of professional communication is considered. The types of interactions between people in everyday life (ritual and entertainment interaction, joint purposeful activity, no interaction, game and interpersonal interaction) are identified. Traditional and specific forms and methods of teaching are written out. All interactive technologies carried out by means of information technologies are conventionally divided into four groups, depending on the form of educational activity appropriate for their use (pair (work of the subject with the teacher or peers one on one by means of Information Technologies); frontal (the teacher simultaneously teaches a group of subjects by means of Information Technologies); group or cooperative (all subjects teach each other by means of Information Technologies); individual (independent work of the subject using Information Technologies)). In the higher education institution, future specialists should learn knowledge, acquire skills on the basic rules of the culture of professional communication and methods of interaction and their effective use, which is possible with the use of Information Technologies. Recommendations for optimal professional communication have been developed that help you express your thoughts easily and beautifully, and conduct a dialogue in a relaxed and harmonious way.

• International Journal of Computer Science & Network Security
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• 제24권7호
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• pp.157-169
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• 2024

The article presents an analysis of innovative teaching technologies as a way to increase students' competitiveness. The author found that innovative technologies in education are information and communication technologies relying on computer-based learning. The structure, content of educational software, organization of Web-space are important when using innovative teaching technologies in English classes. We conducted the study in several stages: comparative analysis, synthesis, classification and systematization of the results of psychological and pedagogical, educational and methodological research; study of legislative acts, periodicals in order to identify the state of the research issue, and determining the directions of its solution, as well as subject, goal and objectives of the study. We used modelling to create situations of foreign language professional communication of future IT specialists. Empirical methods involved questionnaires used for identifying the motives of professional development and determining the features of the educational activities of future IT specialists in the process of training. The methods of mathematical statistics allowed to scientifically describe and systematize the obtained data, to identify the quantitative relationship between the studied phenomena, to analyse and summarize the results. We conducted a socio-psychological study during 2016 - 2019. It involved 255 first- and fourth-year students of National Technical University of Ukraine "Igor Sikorsky Kyiv Poly-technic Institute." Innovative information and communication technologies that improve the educational and cognitive activity of students, as well as increase the level of their knowledge have become important in teaching a foreign language in higher educational institutions. These technologies include MOODLE - Modular Object-Oriented Dynamic Learning Environment, business game, integrated pedagogical technology, case study technology. Thus, the information-rich learning process in combination with the use of innovative technologies, well-organized e-learning, interactive training courses, multimedia tools improves the program of teaching and learning foreign languages in general, and English in particular, improves the level of knowledge of future IT specialists and motivation to study and learn foreign languages, allows students to use a variety of authentic materials. We state that all these factors influence the process of individualization of learning and contribute to the successful mastery of a foreign language.

• Tuberculosis and Respiratory Diseases
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• 제79권4호
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• pp.257-266
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• 2016

Background: Idiopathic pulmonary fibrosis is a common interstitial lung disease; it is a chronic, progressive, and fatal lung disease of unknown etiology. Over the last two decades, knowledge about the underlying mechanisms of pulmonary fibrosis has improved markedly and facilitated the identification of potential targets for novel therapies. However, despite the large number of antifibrotic drugs being described in experimental pre-clinical studies, the translation of these findings into clinical practices has not been accomplished yet. NADH:quinone oxidoreductase 1 (NQO1) is a homodimeric enzyme that catalyzes the oxidation of NADH to $NAD^+$ by various quinones and thereby elevates the intracellular $NAD^+$ levels. In this study, we examined the effect of increase in cellular $NAD^+$ levels on bleomycin-induced lung fibrosis in mice. Methods: C57BL/6 mice were treated with intratracheal instillation of bleomycin. The mice were orally administered with ${\beta}$-lapachone from 3 days before exposure to bleomycin to 1-3 weeks after exposure to bleomycin. Bronchoalveolar lavage fluid (BALF) was collected for analyzing the infiltration of immune cells. In vitro, A549 cells were treated with transforming growth factor ${\beta}1$ (TGF-${\beta}1$) and ${\beta}$-lapachone to analyze the extracellular matrix (ECM) and epithelial-mesenchymal transition (EMT). Results: ${\beta}$-Lapachone strongly attenuated bleomycin-induced lung inflammation and fibrosis, characterized by histological staining, infiltrated immune cells in BALF, inflammatory cytokines, fibrotic score, and TGF-${\beta}1$, ${\alpha}$-smooth muscle actin accumulation. In addition, ${\beta}$-lapachone showed a protective role in TGF-${\beta}1$-induced ECM expression and EMT in A549 cells. Conclusion: Our results suggest that ${\beta}$-lapachone can protect against bleomycin-induced lung inflammation and fibrosis in mice and TGF-${\beta}1$-induced EMT in vitro, by elevating the $NAD^+$/NADH ratio through NQO1 activation.

• 식물병연구
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• 제21권1호
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• pp.27-31
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• 2015

2014년 봄 전라북도농업기술원 시험포장에서 콜라비 시들음병이 관찰되었다. 병징은 생육초기 건전주에 비해 생육이 더디고 신초부터 시들음 증상이 나타나기 시작하였으며, 오래된 잎은 마르고 뒤틀리다가 더 이상 생육을 하지 않고 고사하였다. 감자한천배지에서 윤문형 핑크빛-흰색으로 양모상으로 생육하였다. 대형분생포자는 길이가 짧고 낫모양-일자형으로 세포벽이 얇고 주로 3개의 격벽이 있었다. 소형분생포자는 균사로부터 짧은 monophialides 위에 많이 형성되었으며, 투명하고, 난형 내지 타원형, 격벽이 없거나 1개이며, 드물게 2개이며, 크기는 $4-11{\times}2.5-5{\mu}m$이었다. 균학적 특징, 병원성 검정, ITS rDNA, TEF 염기서열 비교분석 등의 결과를 바탕으로 이 병은 우리나라에서 지금까지 보고되지 않은 Fusarium oxysporum에 의한 콜라비 시들음병으로 명명하고자 한다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권6호
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• pp.880-884
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• 2001

Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.

• 인지과학
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• 제10권2호
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• pp.27-34
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• 1999

이 연구의 목적은 대화행위 개념을 이용하여 독일어 일정협의 대화에서 나타나는 대화의 일반적인 흐름을 밝히는 데에 있다. 이 연구의 기본가정은 대화행위 개념의 도입이 자동번역시스템의 성능향상에 기여한다는 것이다. 그 이유는 통사적인 분석이나 의미적인 분석이 해결하지 못하는 문제들을, 문맥지식을 활용하여 해결하는 데에 있어 대화행위개념이 매우 유용하기 때문이다. 이 대화행위 개념의 하위분류작업은 독일에서 수행되고 있는 VERBMOBIL 프로젝트에서 이루어진 것이다. 본 연구에서는 전문가들에 의해 전사된 실제 대화 자료들을 대화행위의 개념을 활용하여 분석하고, 하나의 대화차례 안에서 발생하는 대화행위들의 연쇄와 각각의 대화차례 사이에서 발생하는 대화행위 연쇄를 조사했다. 여기서 분석대상으로 삼은 전사자료가 다른 연구에서 제시된 전사 자료와는 다소 차이를 보였기 때문에, 본 연구에서는 연쇄 개념을 대화차례사이에 뿐만 아니라 하나의 대화차례 내부까지 적용시켰다. 그리고 대화행위들간의 연쇄관계를 조사함으로써‘일정협의 대화’의 대화 흐름 도를 도출하였다. 이 대화 흐름 도는 통계분석 절차를 통해 가장 빈도수가 높게나온 대화행위 연쇄들을 토대로 만들어진 모형이기 때문에, 일상적인‘일정협의 대화’에 대한 대표성을 갖는다고 볼 수 있다.

• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.152-161
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• 2015

CodY is a highly conserved protein in low G+C gram-positive bacteria that regulates genes involved in sporulation and stationary-phase adaptation. Bacillus thuringiensis is a grampositive bacterium that forms spores and parasporal crystals during the stationary phase. To our knowledge, the regulatory mechanism of CodY in B. thuringiensis is unknown. To study the function of CodY protein in B. thuringiensis, BMB171codY^- was constructed in a BMB171 strain. A shuttle vector containing the ORF of cry1Ac10 was transformed into BMB171 and BMB171codY^-, named BMB171cry1Ac and BMB171codY^-cry1Ac, respectively. Some morphological and physiological changes of codY mutant BMB171codY^-cry1Ac were observed. A comparative proteomic analysis was conducted for both BMB171codY^-cry1Ac and BMB171cry1Ac through two-dimensional gel electrophoresis and MALDI-TOF-MS/MS analysis. The results showed that the proteins regulated by CodY are involved in microbial metabolism, including branched-chain amino acid metabolism, carbohydrate metabolism, fatty acid metabolism, and energy metabolism. Furthermore, we found CodY to be involved in sporulation, biosynthesis of poly-β-hydroxybutyrate, growth, genetic competence, and translation. According to the analysis of differentially expressed proteins, and physiological characterization of the codY mutant, we performed bacterial one-hybrid and electrophoretic mobility shift assay experiments and confirmed the direct regulation of genes by CodY, specifically those involved in metabolism of branched-chain amino acids, ribosomal recycling factor FRR, and the late competence protein ComER. Our data establish the foundation for in-depth study of the regulation of CodY in B. thuringiensis, and also offer a potential biocatalyst for functions of CodY in other bacteria.

• 대한간호학회지
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• 제19권1호
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• pp.24-39
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• 1989

This study was conducted to investigate the present situation and problems related to the use of nursing diagnosis in practice. The data were obtained from 332 subjects (27 director of nursing service, 302 staff nurses) who worked in university hospitals in Korea from July through August 1988 using a mailed questionnaire. Data were analyzed by frequency, X$^2$ test and t-test. The findings were as follows ; 1, Clinical use of nursing diagnosis by directors of nursing service and staff nurses. 1) The majority of the nursing departments (88.9%) conducted group education on nursing diagnosis during the last 5 years and 81.5% of them kept a record format for nursing diagnosis : 88.9% of them had had prior experience with the nursing diagnosis. 2) Most of nurses (97.0%) had received education on nursing diagnosis. 2. Factors related to the clinical use of nursing diagnosis in nursing service departments and by staff nurses. 1) The one factor related to the use of nursing diagnosis in the nursing service department was the existence of a record. 2) Factors related to the use of nursing diagnosis by the staff nurses were the organization style of the nursing service department, group education during the last 5 years, existence of a record, the attitude of the director of nursing service, and prior experience of the use of the nursing diagnosis as characteristics of nursing service department and educational experience of nursing diagnosis as a character of nurse. 3. Problems with the use of nursing diagnosis. 1) The primary problem was the lack of time and personnel (mean : 3.757) ; the second problem was the lack of knowledge and will to use nursing diagnosis in practice by the staff nurses(mean : 3.546). 2) There was no significant difference in problems expressed by the director of nursing services and the nurses. The majority of nurses who worked in the university hospitals expressed interest in and concern about the use of nursing diagnosis. Most of the nurses had had education about on nursing diagnosis but use in practice was limited. The primary problem was lack of time and manpower. Strategies for improving use of the nursing diagnosis in practice : 1) Strengthening the education about nursing diagnosis and a holistic approach to understanding human beings. 2) Develop protocols for the use of nursing diagnosis. 3) Eliminate the language barrier regarding nursing diagnosis by translation into in Korean. 4) Decentralization of the nursing service to promote accountability by individual nurses for use of nursing diagnosis.

• 식물병연구
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• 제24권1호
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• pp.75-80
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• 2018

2014년부터 2016년까지 전라북도농업기술원(익산)과 국립원예특작과학원 온난화대응농업연구소(제주)에서 패션프루트 시들음병이 관찰되었다. 병징은 생육 초기 건전주에 비해 생육이 더디고 새순부터 시듦 증상이 나타나기 시작하였으며, 오래된 잎은 마르고 뒤틀리다가 더 이상 생육하지 않고 말라 죽었다. 감자한천배지에서 균사 생육은 직경이 35 mm/7일이었으며, 핑크빛-흰색으로 양모상이었다. 병원균의 대형분생포자는 무색으로 투명하며, 길이가 짧고 굴곡진 모양-일자형으로 세포벽이 얇고 주로 2-3개의 격벽이 있었다. 소형분생포자는 균사로부터 monophialides 위에 형성되었으며, 투명하고, 난형-타원형이며, 격벽이 없거나 1개인데, 드물게 2개인 경우도 있었고, 크기는 $3-12{\times}2.5-6{\mu}m$이다. 균학적 특징, 병원성 검정, ITS rDNA, $EF-1{\alpha}$ 염기서열 비교분석 등의 결과를 바탕으로 이 병은 우리나라에서 지금까지 보고되지 않은 Fusarium oxysporum에 의한 '패션프루트 시들음병'으로 명명하고자 한다.

• 과학교육연구지
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• 제38권3호
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• pp.543-554
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• 2014

이 연구의 목적은 현재 이루어지고 있는 대한민국 과학교사들이 실시하고 있는 동티모르에서의 과학교사 실험 연수에 대해 대한민국과 동티모르 교사들이 어떻게 인식하고 있는지 알아보는 데 있다. 이 연구를 위해 참여관찰과 설문 조사, 면담이 이루어졌다. 연구 결과, 대한민국 과학 교사들이 동티모르 과학교사 연수를 실시하는 이유는 동티모르 과학교사들의 자발적인 성장을 돕기 위한 것이며, 과학교사로써 국제적인 재능 기부 형태의 봉사활동 수행하는데 있는 것으로 나타났다. 대한민국 과학교사들은 연수를 진행하면서 언어 및 실험 재료 등의 현지화가 절실하다는 반응을 보였다. 동티모르 과학교사 실험연수가 체계를 갖추기 위해서는 동티모르의 과학과 교육과정과 교육 현황에 대한 정확한 자료, 현지 과학교사들의 필요가 무엇인지 파악, 연수 결과에 대한 피드백이 중요하다고 하였다. 연수에 참가한 동티모르 과학교사들은 10년 이내, 20-30대의 교사들이 많았고, 초등보다는 중등교사들의 참여가 많았다. 동티모르의 과학교사들이 연수를 통해 기대하는 것은 과학에 대한 전문성의 심화에 초점이 맞추어져 있었다. 연수 참가시 어려운 점은 언어, 비용, 거리 등의 요인이었다. 연수 프로그램에 대한 동티모르 교사들의 평가는 모든 프로그램이 재미있고, 유용하고 학생들에게 필요한 프로그램이라는 것을 강력히 표현하고 있었다. 또한, 동티모르 과학교사 연수의 최종 목표는 동티모르 교사들의 자생적인 연수 능력 획득에 있었는데, 이러한 가능성에 대한 실마리를 얻었다는 것이 중요한 의미이다.