• Animal cells and systems
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• v.2 no.4
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• pp.483-488
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• 1998

We previously found that a potent gonadotropin-releasing hormone (GnRH) agonist, buserelin, decreases GnRH promoter activity together with GnRH mRNA level, providing evidence for an autoregulatory mechanism operating at the level of GnRH gene transcription in immortalized GT1-1 neuronal cells. To examine whether agonist-induced decrease in GnRH mRNA level requires the continuous presence of buserelin, we performed a pulse-chase experiment of buserelin treatment. Short-term exposure (15 min) of GT1-1 neuronal cells to buserelin ($10{\mu}M$) was able to decrease GnRH mRNA levels when determined 24 h later. When GT1-1 cells were treated with buserelin ( $10{\mu}M$) for 30 min and then incubated for 1, 3, 6, 12, 24, and 48 h after buserelin removal, a significant decrease in GnRH mRNA levels was observed after the 12 h incubation period. These data indicate that inhibitory signaling upon buserelin treatment may occur rapidly, but requires a long time (at least 12 h) to significantly decrease the GnRH mRNA level. To examine the possible involvement of de novo synthesis and/or mRNA stability in buserelin-induced decrease in GnRH gene expression, actinomycin D ($5{\mu}m/ml$), a potent RNA synthesis blocker, was co-treated with buserelin. Actinomycin D alone failed to alter basal GnRH mRNA Revel, but blocked the buserelin-induced decrease in GnRH mRNA level at 12 h of post-treatment. These data suggest that buserelin may exert its inhibitory action by altering the stability of GnRH mRNA. Moreover, a polvsomal RNA separation by sucrose gradient centrifugation demonstrated that buserelin decreased the translational efficiency of the transcribed GnRH mRNA. Taken together, these results clearly indicate that GnRH agonist buserelin acts as an inhibitory signal at multiple levels such as transcription mRNA stability, and translation.

• Smart Media Journal
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• v.7 no.1
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• pp.37-44
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• 2018

Utilizing sequence control and numerical computing, embedded devices are used in a variety of automated systems, including those at industrial sites, in accordance with their control program. Since embedded devices are used as a control system in corporate industrial complexes, nuclear power plants and public transport infrastructure nowadays, deliberate attacks on them can cause significant economic and social damages. Most attacks aimed at embedded devices are data-coded, code-modulated, and control-programmed. The control programs for industry-automated embedded devices are designed to represent circuit structures, unlike common programming languages, and most industrial automation control programs are designed with a graphical language, LAD, which is difficult to process static analysis. Because of these characteristics, the vulnerability analysis and security related studies for industry automation control programs have only progressed up to the formal verification, real-time monitoring levels. Furthermore, the static analysis of industrial automation control programs, which can detect vulnerabilities in advance and prepare for attacks, stays poorly researched. Therefore, this study suggests a method to present a discussion on an industry automation control program designed to represent the circuit structure to increase the efficiency of static analysis of embedded industrial automation programs. It also proposes a medium term translation technology exploiting LLVM IR to comprehensively analyze the industrial automation control programs of various manufacturers. By using LLVM IR, it is possible to perform integrated analysis on dynamic analysis. In this study, a prototype program that converts to a logical expression type of medium language was developed with regards to the S company's control program in order to verify our method.

• 한국전산유체공학회:학술대회논문집
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• 2009.11a
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• pp.151-158
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• 2009

To implement the insects' flapping flight for developing flapping MAVs(micro air vehicles), the unsteady flow characteristics of the insects' forward flight is investigated. In this paper, two-dimensional FSI(Fluid-Structure Interaction) simulations are conducted to examine realistic flow features of insects' flapping flight and to examine the flexibility effects of the insect's wing. The unsteady incompressible Navier-Stokes equations with an artificial compressibility method are implemented as the fluid module while the dynamic finite element equations using a direct integration method are employed as the solid module. In order to exchange physical information to each module, the common refinement method is employed as the data transfer method. Also, a simple and efficient dynamic grid deformation technique based on Delaunay graph mapping is used to deform computational grids. Compared to the earlier researches of two-dimensional rigid wing simulations, key physical phenomena and flow patterns such as vortex pairing and vortex staying can still be observed. For example, lift is mainly generated during downstroke motion by high effective angle of attack caused by translation and lagging motion. A large amount of thrust is generated abruptly at the end of upstroke motion. However, the quantitative aspect of flow field is somewhat different. A flexible wing generates more thrust but less lift than a rigid wing. This is because the net force acting on wing surface is split into two directions due to structural flexibility. As a consequence, thrust and propulsive efficiency was enhanced considerably compared to a rigid wing. From these numerical simulations, it is seen that the wing flexibility yields a significant impact on aerodynamic characteristics.

• International Journal of Industrial Entomology and Biomaterials
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• v.33 no.2
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• pp.102-107
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• 2016

Stress may be defined as any modification of environmental parameters that leads to a response by biological organisms. Stresses that affect biolpgical structures may be nonthermal, such as ultraviolet radiation, pH, or salinity, or thermal. Temperture is one of the major stresses that all living organism face. The major effects of cold(low emperature) are decrease of membrane fluidity and the stabilization of secondary structures of RNA and DNA in the cells, which may effect the efficiency of translation, transcription, and DNA replication. In this study, we focus on discovering the genes that are expressed by the cold(low temperature) stress in the silkworm. In cold(low temperature) stress test, we found 100% survive from cold stress at $0^{\circ}C$ up to 12h and $-5^{\circ}C$ up to 2h, and then, survive rate was rapidly decrease in silkworm. Thereafter two whole genes have selected by SSH(Suppression subtractive hybridization). (GenBank accession : GQ149511, GQ338156)

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.8
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• pp.1102-1110
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• 2016

The mammalian target of rapamycin (mTOR) regulates cellular processes such as cell growth, metabolism, transcription, translation, and autophagy. Rapamycin is a selective inhibitor of mTOR, and induces autophagy in various systems. Autophagy contributes to clearance and recycling of macromolecules and organelles in response to stress. We previously reported that vitrified-warmed mouse oocytes show acute increases in autophagy during warming, and suggested that it is a natural response to cold stress. In this follow-up study, we examined whether the modulation of autophagy influences survival, fertilization, and developmental rates of vitrified-warmed mouse oocytes. We used rapamycin to enhance autophagy in metaphase II (MII) oocytes before and after vitrification. The oocytes were then subjected to in vitro fertilization (IVF). The fertilization and developmental rates of vitrified-warmed oocytes after rapamycin treatment were significantly lower than those for control groups. Modulation of autophagy with rapamycin treatment shows that rapamycin-induced autophagy exerts a negative influence on fertilization and development of vitrified-warmed oocytes.

• Journal of Welding and Joining
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• v.14 no.1
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• pp.99-106
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• 1996

Development of an automatic TIG welding system incorporating a vision sensor and torch control mechanism leads to an improved welding quality and greater production efficiency. The automatic welding system should be greatly restricted in its size and weight for the LNG(Liquefied Natural Gas) storage tank and also provide a unique torch rotating mechanism which keeps the torch tip in the constant position while the angle is changed continuously to maintain the welding torch substantially perpendicular to the weld line. The developed system is driven by two translation axes X, Z and one rotational axis. A moving line window method is adopted to the image recognition of the corrugated membranes with specular reflection. This method decides original laser stripe patterns in image which is affected by multi-reflection. A self-teaching algorithm, which guides the automatic welding machine with the information provided by the CCD camera without any previous learning of a reference trajectory, was developed for tracking the corrugated membrane of the LNG tank along the weld line.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.35 no.7
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• pp.627-632
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• 2007

Dual quaternion is efficient methodology to express rotation and translation of the vehicle's movements in the unified frame work. Recently, a strapdown inertial navigation algorithm based on dual quaternion was introduced. By comparing and analyzing the classical and dual-quaternion algorithms, this paper proposes a new strapdown inertial navigation algorithm that maintains the accuracy benefit of the dual-quaternion algorithm with considerable computational reduction. Simulation results show the efficiency of the proposed hybrid strapdown navigation algorithm.

• Journal of Information Display
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• v.13 no.1
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• pp.17-20
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• 2012

The current panels for liquid crystal display (LCD) TV sets are all characterized by pins placed on two sides. To manufacture one type of panel, a dedicated mask is needed, and the production line should be started once. Moreover, the whole panel needs to be removed if a dead pixel is found. If the panel, however, will first be fabricated with pins placed on four sides and then divided by a cross-cut into four equal-sized panels with pins placed on two sides, one set of masks can be used to manufacture two types of LCD panels, which was referred to by the authors as dual mode. In this paper, the concept of the dual mode and its differences from the conventional way of producing panels are introduced. Its advantages in boosting production efficiency, improving the product's good rate, and lowering the production cost are also described based on case studies. Of particular importance is the fact that the dual mode is very suitable for the trial production of very-large-area LCD TV sets.

• Journal of the Korean Institute of Telematics and Electronics
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• v.25 no.10
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• pp.1193-1201
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• 1988

In this paper, we describe the syntactic and semantic parsing methods which use the case frames. The case structures based on obligatory cases of verbs. And, we use a small set of partial-garammar rules based on simple sentence to represent such case structures. Also, we enhance the efficiency by constructing independent procedure for particle classification and ambiguity resolution of major particle considering the importance of Japanese particle process in the generation. And we construct the generation table considering the combination possibility between the verbs and auxiliary verbs for processing the termination phrase. Therefore we can generate more natural translated sentence according to unique decision with information of syntactic analysis and simplify the generating process.

• KIEAE Journal
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• v.15 no.5
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• pp.95-105
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• 2015

Purpose: This paper presents a framework development for BIM (Building Information Modeling)-based OOPM (Object-Oriented Physical Modeling) for Building Thermal Simulation. The framework facilitates decision-making in the design process by integrating two object-oriented modeling approaches (BIM and OOPM) and efficiently providing object-based thermal simulation results into the BIM environment. Method: The framework consists of a system interface between BIM and OOPM-based building energy modeling (BEM) and the visualization of simulation results for building designers. The interface enables a BIM models to be translated into OOPM-based BEM automatically and the thermal simulation from the created BEM model immediately. The visualization module enables the simulation results to be presented in BIM for building designers to comprehend the relationships between design decisions and the building performances. For the framework implementation, we utilized the Modelica Buildings Library developed by the Lawrence Berkeley National Laboratory as a thermal simulation solver. We also conducted an experiment to validate the framework simulation results and demonstrate our framework. Result: This paper demonstrates a new methodology to integrate BIM and OOPM-based BEM for building thermal simulation, which enables an automatic translation BIM into OOPM-based BEM with high efficiency and accuracy.