• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.6
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• pp.571-575
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• 2005

A simple, but effective on-line method for estimating the mycelial cell mass concentration from agitation speed data, a most readily-available process variable, has been developed for DO-stat cultures of Agaricus blazei. The dynamic change of dissolved oxygen concentration (DOC) in the initial transient period and the change in yield were considered in the development of the estimation algorithm or estimator. Parameters in the estimation algorithm were calculated from the agitation speed data at 20% of DOC. The proposed estimator could accurately predict the cell mass concentration regardless of DOC levels in the tested range of $10{\sim}40%$, showing a good extrapolation capability.

• The Transactions of the Korean Institute of Electrical Engineers A
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• v.54 no.10
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• pp.487-495
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• 2005

This paper reviews the characteristic of thermal and temperature of oil field cable in transient state such as grounding fault and lightning surge. For analysis in various conditions, many actual underground power cable systems are modeled using ATP. These results are applied for the examination of temperature increase when the single line to ground fault by the breakdown of insulation part and hitting of lightning surge are occurred. The inner part temperature of OF cable is analysed according to the various kinds of cable using the thermal model of transient state. The temperature increase of sheath and crossbonded lead bv fault current is also analysed using IEC 60949.

• Animal cells and systems
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• v.2 no.1
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• pp.117-122
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• 1998

D-raf, a Drosophila homolog of the human c-raf-1, is known as a signal transducer in cell proliferation and differentiation. A previous study found that the D-raf gene expression is regulated by the DNA replication-related element (DRE)/DRE-binding factor (DREF) system. In this study, we found the sequences homologous to transcription factor C/EBP, MyoD, STAT and Myc recognition sites in the D-raf promoter. We have generated various base substitutional mutations in these recognition sites and subsequently examined their effects on D-raf promoter activity through transient CAT assays in Kc cells with reporter plasmids p5'-878DrafCAT carrying the mutations in these binding sites. Through gel mobility shift assay using nuclear extracts of Kc cells, we detected factors binding to these recognition sites. Our results show that transcription factor C/EBP, STAT and Myc binding sites in D-raf promoter region play a positive role in transcriptional regulation of the D-raf gene and the Myo D binding site plays a negative role.

• 한국전산유체공학회:학술대회논문집
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• 2007.04a
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• pp.64-67
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• 2007

In the present study, the wind pressure transients on platform screen door in side platforms caused by passing trains have been investigated numerically. The transient compressible 3-D full Navier-Stokes solution is obtained with actual operational condition of subway train and the moving mesh technique is adopted considering the train movement. To achieve more accurate analysis, the entrance and exit tunnel of platform are included in a computational domain and detailed shape of train is also modeled Numerical analyses were conducted on five operational condition which are different velocity variation of subway train, existence of stationary train and passing each other trains. The results show that pressure load on platform screen door is maximized when the two trains are passing each other. It is also seen from the computational results that the maximum pressure variation was found to be satisfactory to various foreign standards.