• Journal of Plant Biotechnology
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• v.2 no.2
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• pp.89-96
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• 2000

For large DNA fragment transformation in dicots and monocots, BIBAC2 vector system was applied to Arabidopsis thaliana and Oryza sativa L. cv. Jinmi as a model plant, respectively. For Arabidopsis, the Th1 gene in T23L3 BAC clone whose size is about 90 kb was used as the target gene source for transformation. Because T23L3 BAC clone was originally constructed in pBelloBAC11, the target gene was reconstructed into BIBAC2. As the results of reconstruction, 476 colonies were survived in selection medium containing 40 mg/L kanamycin. In colony hybridization analysis, 24 out of 476 colonies exhibited positive signals. In the pulsed-field gel electrophoresis analysis, 11 out of 24 positive clones exhibited the band at the location of 90 kb. In Southern hybridization, positive signal band at the location of 90 kb was observed in all 11 transformants. Using these verified clones, Agrobacterium-mediated transformation was applied to Arabidopsis thaliana th1-201 mutant for genetic complementation test. Twelve thousands T$_1$ seeds were harvested, and antibiotic selection test is being analyzed to verify whether these seeds were transformed. for rice, COR356 that contains 150 kb human genomic DNA in a BIBAC2 vector was used as the target gene. As the results of transformation, 151 out of 210 co-cultivated calli were survived in selection medium containing 5 mg/L hygromycin, and 45 out of 151 survived calli were regenerated into plants. Transformation efficiency was 21.6%. Progeny test using 71 seeds is being analyzed now. These results provide the potential that large DNA fragments can be transferred into both dicots and monocot by Agrobacterium-mediate d transformation system.

• Journal of the Korean Society of Clothing and Textiles
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• v.30 no.1 s.149
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• pp.167-175
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• 2006

The purpose of this study is to clarify the expansion of functions of clothes by analyzing the characteristics of transformation, and to forecast future trends in fashion through systematization of the aforementioned analyses. Analysis of 20th century Modernism and Post-Modernism in a sociocultural sense confirms that transformation in clothes was formed by environmental, functional, deconstructive, and expressive factors. In this sense, the formative factors mentioned above conceptually include nomadic characteristics, usefulness, irregularity, and expressiveness. The nomadic characteristics found in clothes transformation signify the change of clothes into environmental nomadic everyday implements, used as tools for the body. Usefulness of clothes means that it is worn for variability, multipurpose multi-functionality, and combined multiple use. Irregularity means the clothes can change indefinitely, according to random manipulation on the wearer's part. Last of all, expressiveness conveys the designer's internal sensitivity and imagination onto an external object through the induction of various expressive factors.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.11
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• pp.1601-1605
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• 2012

Three-phase voltage and current is applied to the three-phase alternating current motors which are commonly used in industry. Three phase variables of a, b, c are converted into d, q, 0 axis and the AC machines are modeled and analyzed. Basically the coordinate transformation or d-q transformation is used for convenience, a few steps are needed to analyze the motor performances - separating d and q components, establishing each equivalent circuit, and solving the differential equations of the circuits. In this study, a modeling technique of induction motor using complex vector is proposed and it can explain the induction motor physically. This method does not need the separating process of d and q components. With this technique, the model becomes simple, is easy to understand in physical, and can get the same results with those from the other models. These simulation results of the proposed model are compared with them for the conformation of the proposed method.

• The Mathematical Education
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• v.17 no.2
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• pp.1-13
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• 1979

This study aimed at studying the sequence of the Figure Transformation Learning, inquiring relationship among these transformations and then researching whether there is the difference of the learning ability or not between by teaching them as it is independent and by teaching them as it is contains. (Hypothesis 1) It may be more effective to teach The Sequence of Transformation Learning by beginning with peculiar field, ending with general field than vice versa At the result of verification-C $R_{M}$=2.59, 0.005$R_{M}$=5.19, p<0.005-significant difference appeared. It is proved more effective to teach the Figure Transformation Learning the way it contains than the way it is independent. Synthesizing two hypothesises of the above, the conclusion is following The Figure Transformation Learning should be taught by beginning with peculiar field. ending with general field (congruent transformationlongrightarrowsimilar transformationlongrightarrowprojective transformationlongrightarrowtopological transformation). To teach it the way it contains is more effective.ive.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.3
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• pp.187-192
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• 2014

Alfalfa (Medicago sativa L.) is one of the most important forage legumes in the world. It has been demanded to establish the efficient transformation system in commercial varieties of alfalfa for forage molecular breeding and production of varieties possessing new characteristics. To approach this, genetic transformation techniques have been developed and modified. This work was performed to establish conditions for effective transformation of commercial alfalfa cultivars, Xinjiang Daye, ABT405, Vernal, Wintergreen and Alfagraze. GUS gene was used as a transgene and cotyledon and hypocotyl as a source of explants. Transformation efficiencies differed from 0 to 7.9% among alfalfa cultivars. Highest transformation efficiencies were observed in the cultivar Xinjiang Daye. The integration and expression of the transgenes in the transformed alfalfa plants was confirmed by polymerase chain reaction (PCR) and histochemical GUS assay. These data demonstrate highly efficient Agrobacterium transformation of diverse alfalfa cultivars Xinjiang Daye, which enables routine production of transgenic alfalfa plants.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.1
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• pp.61-68
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• 2004

A reproducible transformation system via optimized regeneration media for Korean rice cultivars was established using Agrobacterium tumefeciens LBA4404 (pSBM-PPGN; gusA and bar). Although japonica rice genotypes were easier to produce transgenic plants compared to Tongil type cultivars, transformation efficiencies were not always correlated with regeneration efficiencies of non-transgenic callus on the control medium. Regeneration efficiencies of Donganbyeo, Ilmibyeo, and Manchubyeo were over 50% in non-transgenic control, however, transformation efficiencies were significantly low when only sucrose was added to the media as a carbon source. However, the medium, MSRK5SS-Pr (or MSRK5SM-Pr), that contains $5\textrm{mgL}^{-1}$ kinetin, $0.5\textrm{mgL}^{-1}$ NAA, 2 % sucrose (or maltose), 3% sorbitol, and $500\textrm{mgL}^{-1}$ proline, was the most efficient not only for regeneration of non-transgenic callus but also for regeneration of transgenic callus in the presence of L-phosphinotricin (PPT). Average transformation efficiencies of 16 Korean rice cultivars were significantly enhanced by using the optimized medium from 1.5% to 5.8% in independent callus lines and from 2.9% to 19.4% in tromsgenic plants obained. Approximately 98.9% (876 out of 885) transgenic plants obtained on optimized media showed basta resistance. Stable integration, inheritance and expression of gusA and bar genes were continued by GUS assay and PCR and Southern analysis of the bar gene. With Pst1 digestion of genomic DNA of transgenic plants, one to five copies of T-DNA segment were observed; however, 76% (19 out of 25 transgenic plants) has low copy number of T-DNA. The transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

• Journal of Plant Biotechnology
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• v.7 no.4
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• pp.219-223
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• 2005

We investigated chemical-wounding effect on Agrobacterium-mediated Chinese cabbage transformation via vacuum infiltration. Pre-germinated or germinating Chinese cabbage seeds were infiltrated with Agrobacterium tumefaciens LBA4404 cells carrying either GUS gene (pBI121) or hepatitis B virus surface antigen DNA (pBIHBsAg). Prior to agroinfiltration process, the seeds were soaked in sodium hydrosulfite (SHS) solution or just in sterile water as a control. Comparative transformation efficiency was determined by both of histochemistry and ELISA. We could demonstrate that SHS solution treatment especially to 1-day or 2-days old germinating seeds efficiently improved transformation process, and therefore, transient expression level. This strongly indicated that Agrobacterium infection could be facilitated indeed by SHS-causing wounds on Chinese cabbage seeds.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.1263-1267
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• 2007

A more complete two-dimensional vertical numerical model has been developed to describe the saltwater intrusion in an estuary. The model is based on the previous studies in order to obtain a better accuracy. The non-linear terms of the governing equations are analyzed and the $\sigma$-coordinate system is employed in the vertical direction with full transformation which is recently issued in several studies because numerical errors can be generated during the coordinate transformation of the diffusion term. The advection terms of the governing equations are discretized by an upwind scheme in second-order of accuracy. By employing an explicit scheme for the longitudinal direction and an implicit scheme for the vertical direction, the numerical model is free from the restriction of temporal step size caused by a relatively small grid ratio. In previous researches, some terms induced from the transformation have been intentionally excluded since they are asked the complicate discretization of the numerical model. However, the lack of these terms introduces significant errors during the numerical simulation of scalar transport problems, such as saltwater intrusion and sediment transport in an estuary. The numerical accuracy attributable to the full transformation is verified by comparing results with a previous model in a simply sloped topography. The numerical model is applied to the Han River estuary. Very reasonable agreements for salinity intrusion are observed.

• Journal of Institute of Control, Robotics and Systems
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• v.19 no.12
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• pp.1145-1151
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• 2013

Kinematic modeling is a prerequisite for motion planning and the control of mobile robots. In this paper, we proposed a new method of kinematic modeling for a type of mobile robot based on differential motion transformation. The differential motion implies a small translation and rotation in three-dimensional space in a small time interval. Thus, transformation of the differential motion gives the velocity relationship, i.e., Jacobian between two coordinate frames. Since the theory of the differential motion transformation is well-developed, it is useful for the systematic velocity kinematic modeling of mobile robots. In order to show the validity for application of the differential motion transformation, we obtained velocity kinematic models for a type of exemplar mobile robot including spherical ballbots.

• Proceedings of the KSRS Conference
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• v.2
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• pp.856-859
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• 2006

In river with bar, the characteristics of its physical conditions have a close relationship with bar morphology. In this paper, a monitoring approach of bar transformation in the Han River Estuary is presented using RADARSAT/SAR Images. The estuary is divided into North and South Korea and its area is blocked by CCL(Civil Control Line). Satellite remote sensing, therefore, is uniquely suited to monitoring bar transformation. Based on SAR signatures for bars, bar transformation is investigated from 2000 to 2005, and monitoring of suspended-silt transportations from terrestrial runoff is tried to understand the morphology during the events of severe rain storm. SAR data did not reveal clearly the bar locations because of most of data acquisitions during high tides from 6.8 m to 9.0 m. Even though the problem, it could be said that in the estuary vegetated area and natural levees are developed well, but bars and shifted after an event like a flood. It is also showed that suspended solids such as silt transported through the estuary could contribute highly to a sedimentation environment around Incheon.