• Plant Resources
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• v.7 no.1
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• pp.39-46
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• 2004

To demonstrate the importance of transformation efficiency in independent event, molecular and cytogenetic analysis were conducted with genomic DNA and chromosome of transgenic plants produced by Agrobacterium tumefeciens LBA4404 (pSBM-PPGN: gusA and bar). Selection ratios of putative transgenic calli were similar in independent experiments, however, transformation efficiencies were critically influenced by the type of regeneration media. MSRK5SS-Pr regeneration mediun, which contains 5 mgL$^{-1}$ kinetin, 2% (w/v) sucrose in combination with 3% (w/v) sorbitol, and 500 mgL$^{-1}$ proline, was efficient to produce transgenic plant of rice from putative transgenic callus in the presence of L-phosphinotricin (PPT). With MSRK5SS-Pr medium, transformation efficincies of Nagdongbyeo were significantly enhanced from 3.7% to 6.3% in independent callus lines arid from 7.3% to 19.7% in plants produced, respectively. Stable integration and expression of bar gene were confirmed by basta herbicide assay, PCR amplification and Southern blotting of bar gene, and fluorescence in situ hybridization (FISH) analysis using pSBM-PPGN as a probe. In Southern blot analysis, diverse band patterns were observed in total 44 transgenic plants regenerated from 20 independent PPT resistant calli showing from one to five copies of T-DNA segments, however, the transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

• Proceedings of the KSRS Conference
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• 2007.03a
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• pp.188-191
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• 2007

The Han River is divided into North and South Korea by NLL(Northern Limit Line) and its area has been blocked by CCL(Civil Control Line) since the Korean War in 1950. Satellite remote sensing, therefore, is uniquely suited to monitoring bar transformation in the region. In river with bar, the characteristics of its physical conditions have a close relationship with bar morphology. In this paper, a monitoring approach of bar transformation in the Han River Estuary is presented using RADARSAT/SAR images from 2000 to 2005 and spatial patterns of bar morphology are presented. it could be said that in the estuary vegetated area and natural levees are developed well, but bars are shifted after an event like a flood. It is also showed that suspended solids such as silt transported through the estuary could contribute highly to a sedimentation environment around Incheon.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1996.11a
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• pp.296-300
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• 1996

This paper presents 3D position estimation algorithm with the images of 2D vision sensors which issues Red Laser Slit light and recieves the line images. Since the sensor usually measures 2D position of corner(or edge) of a body and the measured point is not fixed in the body, the additional information of the corner(or edge) is used. That is, corner(or edge) line is straight and fixed in the body. For the body which moves in a plane, the Transformation matrix between the body coordinate and the reference coordinate is analytically found. For the 3D motion body, linearization technique and least mean squares method are used.

• Journal for History of Mathematics
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• v.29 no.3
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• pp.157-172
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• 2016

Sophus Lie's research is regarded as one of the most important mathematical advancements in the $19^{th}$ century. His pioneering research in the field of differential equations resulted in an invaluable consolidation of calculus and group theory. Lie's group theory has been investigated and constantly modified by various mathematicians which resulted in a beautifully abstract yet concrete theory. However Lie's early intentions and ideas are lost in the mists of modern transfiguration. In this paper we explore Lie's early academic years and his object of studies which clarify the ground breaking ideas behind his theory.

• Journal of the Korea Society of Computer and Information
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• v.7 no.1
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• pp.54-61
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• 2002

In this paper, we implement the XML modeling and data modeling by the class diagram that is made by the use case after drawing out the sequence diagram. For the XML modeling, the guiding line to map the UML classes on the XML document will be presented. Then, an example to generate the ie DTD from the UML classes will be shown under the presented guiding line. In the data modeling, the transformation method from the In classes into the object-oriented database also will be proposed. Finally, we will give an example developed by the Proposed transformation method.

• Proceedings of the KIEE Conference
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• 1991.11a
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• pp.105-107
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• 1991

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.148.2-149
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• 2003

Cucumber fruit mottle mosaic tobamovirus (CFMMV) causes severe mosaic symptoms with yellow mottling on leaves and fruits, and occasionally severe wilting of cucumber plants. No genetic source of resistance against this virus has been identified. The genes coding for the coat protein or the putative 54-kDa replicase were cloned into binary vectors under control of the SVBV promoter. Agrobacterium-mediated transformation was peformed on cotyledon explants of a parthenocarpic cucumber cultivar with superior competence for transformation. R1 seedlings were evaluated for resistance to CFMMV infection by lack of symptom expression, back inoculation on an alternative host and ELISA. From a total of 14 replicase-containing R1 lines, 8 exhibited immunity, while only 3 resistant lines were found among a total of 9 CP-containing lines. Line 144 homozygous for the 54-kDa replicase was selected for further resistance analysis. Line 144 was immune to CFMMV infection by mechanical and graft inoculation, or by root infection following planting in CFMMV-contaminated soil. Additionally, line 144 showed delay of symptom appearance following infection by other cucurbit-infecting tobamoviruses. Infection of line 144 plants with various potyviruses and cucumber mosaic cucumovirus did not break the resistance to CFMMV. The mechanism of resistance of line 144 appears to be RNA-mediated, however the means is apparently different from the gene silencing phenomenon. Homozygote line 144 cucumber as rootstock demonstrated for the first time protection of a non-transformed scion from soil inoculation with a soil borne pathogen, CFMMV.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.37-45
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• 2007

The genetic transformation of watermelon by Agrobacterium has been known very difficult and a few successful cases have been reported by obtaining the direct shoot formation. However, since this direct shoot formation is not guaranteed the stable transformation, the stable transformation with reproducibility is required by a different approach such as a callus induced manner. The best conditions for inducing the callus from cotyledon and root explants of watermelon were 2 mg/L zeatin + 0.1 mg/L IAA and 2 mg/L BA + 0.1 mg/L 2,4-D, respectively. The GFP expression in the callus was identified and monitored through fluorescent microscopy after transformation with pmGFP5-ER vector. Paromomycin rather than kanamycin was used for selecting the nptll gene expression because it was more effective to select the watermelon explants. Four different callus types were observed and the solid green callus showed stronger GFP expression. The highest frequency of GFP expression in the callus developed from cotyledon was 9.0% (WM8 inbred line), while the highest frequency from root was 8.3% (WM6 inbred line). The WMV-CP was transformed using the method of GFP transformation and the genetic transformation of WMV-CP was confirmed by PCR and Southern blot analysis. Here we present a system for callus induction of watermelon explant and the callus induced method would facilitate the establishment of stable watermelon transformation.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.4
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• pp.26-32
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• 2013

The earth reflection effect on the induced voltage by line source such as power line occurring induction inteference is analyzed to scrutinize how much it would reduce the induced voltage. Using hankel transformation including bessel function, directly calculation formulae for extracting a refelction coefficient is a most important technical application in this paper since the reflection coefficient on the earth cannot be deduced by a general coefficient calculation formulae according to a plain wave. The electric field is utilized to transform the electromagnetic field into an induced voltage. The composed efficiency to a source induction voltage by an earth reflection is about a range of 60~70% for the axis constellation of each object like observation point, source position and other material parameters.

• Proceedings of the Korea Contents Association Conference
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• 2005.05a
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• pp.401-405
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• 2005

In this paper, we designed data transformation interface for the automation of PCB product. The data designed to CAD does not exchange itself for the assembly line, so we analyzed the information of PCB components designed to CAD and developed human error detect algorithm. We also constructed database for the information of IC components. Developed the algorithm can change to the data suitable for the assembly line of PCB and this system was designed to do addition and revision of the components through the user interface. By automating existing manual processing, we were able to shorten the time of 1-2 days to several minutes and enhance reliability of the data and the efficient assembly line of PCB.