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http://dx.doi.org/10.5010/JPB.2007.34.1.037

Genetic Transformation of Watermelon (Citrullus vulgaris Schard.) by Callus Induction  

Kwon, Jung-Hee (Biotechnology Institute, Nong Woo Bio Co. Ltd.)
Park, Sang-Mi (Biotechnology Institute, Nong Woo Bio Co. Ltd.)
Lim, Mi-Young (Biotechnology Institute, Nong Woo Bio Co. Ltd.)
Shin, Yoon-Sup (Biotechnology Institute, Nong Woo Bio Co. Ltd.)
Harn, Chee-Hark (Biotechnology Institute, Nong Woo Bio Co. Ltd.)
Publication Information
Journal of Plant Biotechnology / v.34, no.1, 2007 , pp. 37-45 More about this Journal
Abstract
The genetic transformation of watermelon by Agrobacterium has been known very difficult and a few successful cases have been reported by obtaining the direct shoot formation. However, since this direct shoot formation is not guaranteed the stable transformation, the stable transformation with reproducibility is required by a different approach such as a callus induced manner. The best conditions for inducing the callus from cotyledon and root explants of watermelon were 2 mg/L zeatin + 0.1 mg/L IAA and 2 mg/L BA + 0.1 mg/L 2,4-D, respectively. The GFP expression in the callus was identified and monitored through fluorescent microscopy after transformation with pmGFP5-ER vector. Paromomycin rather than kanamycin was used for selecting the nptll gene expression because it was more effective to select the watermelon explants. Four different callus types were observed and the solid green callus showed stronger GFP expression. The highest frequency of GFP expression in the callus developed from cotyledon was 9.0% (WM8 inbred line), while the highest frequency from root was 8.3% (WM6 inbred line). The WMV-CP was transformed using the method of GFP transformation and the genetic transformation of WMV-CP was confirmed by PCR and Southern blot analysis. Here we present a system for callus induction of watermelon explant and the callus induced method would facilitate the establishment of stable watermelon transformation.
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1 Chaturvedi R, Bhatnagar SP (2001) High-frequency shoot regeneraton from cotyledon explants of watermelon cv. sugar baby. In Vitro Cell Dev Biol Plant 37: 255-258   DOI   ScienceOn
2 Compton ME, Gray DJ (1999) Shoot organogenesis from cotyledon explants of watermelon. In: Trigiano RN.; Gray DJ (eds) Plant Tissue Culture Concepts and Laboratory Exercises. Ed 2, CRC Press, Boca Raton, FL, pp 149-158
3 Dellaporta, SL, Wood J, Hicks J.B. (1983) A simple and rapid method for plant DNA preparation. Version II. Plant Mol Biol Rep 1: 19-21   DOI
4 Dong JZ, Jia SR (1991) High efficiency plant regeneration from cotyledons of watermelon (Citrullus vulgaris Schrad.) Plant Cell Rep 9: 559-562
5 Ellul P, Rios G, Atares A, Roig LA, Serrano R (2003) The expression of the Saccharomyces cerevisiae HAL1 gene increases salt tolerance in transgenic watermelon (Citrullus lanatus Thunb.) Matsum. & Nakai. Theor Appl Genet 107: 462-469   DOI
6 Franklin G, Lakshmi Sita G (2003) Agrobacterium tumefaciens-mediated transformation of eggplant (Solanum melongena L.) using root explants. Plant Cell Rep 21: 549-554
7 Gaba V, Zelcer A, Gal-On A (2004) Cucurbit biotecholoqy-the importance of virus resistance. In Vitro Cell Dev Biol Plant 40: 346-358   DOI   ScienceOn
8 Kim DH, Lee JM (1997) Shoot regeneration from watermelon explants as affected by cultivars, explant parts and cytokinins. J Kor Soc Hort Sci Suppl 15(1): 72-73
9 Compton ME (1999) Dark pretreatment improves adventious shoot organogenesis from cotyledons of diploid watermelon. Plant Cell Tiss Org Cult 58: 185-188   DOI
10 Compton ME, Gray DJ (1993) Shoot organogenesis and plant regeneration from cotyledons of diploid, triploid and tetraploid watermelon. J Am Soc Hort Sci 118: 151-157
11 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tabacco tissue culture. Physiol Plant 15: 473-479   DOI
12 Roa-Rodriuez and Nottenburg (1999) Nptll gene in combination with paromomycin as a selective agent. Patent EP927765A1
13 Southern, E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503-512   DOI
14 Tricoli DM, Carney KJ, Russell PF, Quemada HD, McMaster RJ, Reynold JF & Deng RZ (2002) Transgenic plants expressing DNA constructs containing a plurality of genes to impart virus resistance. United States Patent No. 6,337,431
15 Simmonds DH, Donaldson PA (2000) Genotype screening for proliferative embryogenesis and biolistic transformation of short-season soybean genotype. Plant Cell Rep 19: 485-490   DOI
16 Cho MA, Song YM, Park YO, Ko SM, Min SR, Liu JR, Lee JH, Choi PS (2005a) Production of transgenic melon from the cultures of cotyledonary-node explant using Agrobacterium-mediated transformation. Kor J Plant Biotechnol 32: 257-262   과학기술학회마을   DOI   ScienceOn
17 Choi JY, Kim SH, Hyung NI (2003) Optimization of Agrobacterium-mediated transformation protocol in watermelon. J Kor Soc Hort Sci Technol Suppl 21: 59
18 Choi PS, Soh WY, Kim YS, Yoo OJ, Liu JR (1994) Genetic transformation and plant regeneration of watermelon using Agrobacterium tumefaciens. Plant Cell Rep 13: 344-348
19 Dabauza M, Bordas M, Salvador A, Roig LA, Moreno V (1997) Plant regeneration and Agrobacterium-mediated trasformation of cotyledon explant of Citrullus colocynthis (L.) Schrad. Plant Cell Rep 16: 888-892   DOI
20 Cho MA, Song YM, Park YO, Ko SM, Min SR, Liu JR, Choi PS (2005b) The use of glufosinate as a selective marker for the transformation of cucumber (Cucumis setivus L.). Kor J Plant Biotechnol 32: 161-165   DOI   ScienceOn
21 Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6: 271-282   DOI   ScienceOn
22 Rashid H, Yokoi K, Toriyama K, Hinata K (1996) Transgenic Plant production mediated by Agrobacterium in Indica rice. Plant Cell Rep 15: 727-730   DOI   ScienceOn
23 Compton ME, Gray DJ, Gaba VP (2004) Use of tissue culture and biotechnology for the genetic improvement of watermelon. Plant Cell Tiss Org Cult 77: 231-243   DOI   ScienceOn
24 Kusano M, Tohyama K, Bae CH, Riu KZ, Lee HY (2003) Plant regeneration and transformation of Kentuchy Bluegrass (Poa pratensis L.) via the plant tissue culture. Kor J Plant Biotechnol 30: 115-121   과학기술학회마을   DOI   ScienceOn
25 Lee HK, Paek KY, Seo YK, Rhee WY (1994) Genotypic effect of watermelon (Citrullus lanatus Thunb.) on organogenesis from shoot tip culture of seedlings. Kor J Plant Tiss Cult 21: 239-246
26 Lee YH, Kim HS, Kim JY, Jung M, Park YS, Lee JS, Choi SH, Her NH, Lee JH, Hyung NI, Lee CH, Yang SG and Ham CH (2004) A new selection method for pepper transformation: callus-mediated shoot formation. Plant Cell Rep 23: 50-58
27 Lee SH, Shon YG, Lee SI, Kim CY, Koo JC, Lim CO, Choi YJ, Han CD, Chung CH, Choe ZR, Cho MJ (1999) Cultivar variability in the Agrobacterium-rice cell interaction and plant regeneration. Physiol Plant 107: 338-340   DOI   ScienceOn