• Journal of Korean Society on Water Environment
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• v.23 no.5
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• pp.705-711
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• 2007

Green quantum-dot nanocrystal (QD525) with anti-microcystin monoclonal antibody was applied for detection of microcystin, a monocyclic peptide hepatotoxin, extracted from the culture of Microcystis aeruginosa. The presence of microcystin in the cell lysate was verified by HPLC analysis with UV absorbance at 238 nm. Microcystis cell extract exhibited fluorescence emission spectra, which peak was around 460 nm because of their complex organic substances. When a spherical QD525 antibody conjugates (10~20 nm in diameter) were bound to the microcystins in the Microcystis cell lysate, the fluorescence intensity of the primary peak at 525 nm diminished while the secondary emission peak at 460 nm slightly increased intensities. It is due to energy transfer from the primary (major) to the secondary (minor) peak, resulting from physical deformation of QD525 and different environmental factors. On the other hand, other cell extracts did not show any fluorescence emission change. This study is very available for detecting and monitoring the microcystin because it is one step assay without washing step and portable spectrophotometer makes on-site measurement possible. For health risk assessment of the microcystin, the reliable and rapid system to detect and quantify microcystin is seriously required.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.26 no.4
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• pp.995-1012
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• 2016

In Internet of Things (IoT) environments, devices or sensors everywhere can automatically collect data without the individual awareness, further combine and share data using ubiquitous network, and thus the development of IoT raises new challenges in respect of personal data protection and privacy. This study aims to identify main issues related to data protection in the IoT and propose adequate measures. We analyzed the types of personal data controllers and processors in IoT and figured out the issues regarding the processing of personal data and the rights to privacy of data subject. Accordingly, we suggested the institutional ways (e.g., establishment of user-friendly notice and flexible consent system, re-identification risk monitoring system, data protection in cross-border transfer, and user education) to improve the situation of personal data protection in IoT and finally proposed the improvement tasks to carry out first based on the degree of urgency and importance.

• Archives of Reconstructive Microsurgery
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• v.8 no.1
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• pp.22-27
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• 1999

While the conventional end-to-end anastomotic technique is accepted as 'the golden standard' for microvascular anastomosis, it is time-consuming and tedious. In an effort to offer faster and safer ways of performing microvascular anastomoses, numerous anastomotic techniques have been proposed, but further refinements in microvascular techniques are still necessary. A 'continuous suture with interrupted knot' technique was devised for faster and safer anastomosis. It has been successfully used in microanastomoses of both artery and vein for free tissue transfer. It is a combination of the interrupted suturing technique and the continuous suturing technique. First, a continuous suture is made with the size of loop decreasing in order, and then the sutures are tied individually from the first loop to the last one as in the conventional interrupted suturing technique. It was applied clinically to fourteen patients over the past ten months and found to be a highly efficient technique that satisfied our needs. This 'continuous suture with interrupted knot' technique has several advantages over other techniques : The operative time is reduced comparing conventional interrupted suture technique. By delaying the tie and with the vessel walls kept separated, the risk of through-stitch can be reduced. Tying all the sutures at one time not only speed up the procedures, but also reduced the surgeon's fatigue. In addition, it has no problem of anastomotic stenosis which is a disadvantage of continuous suture technique. This technique proved to be faster and safer, and has patency equal to that of the conventional end-to-end anastomosis. It is of great help to the surgeon in reducing operative time, especially in clinical situations when many anastomoses are required, or lengthy grafting procedures are undertaken.

• Analytical Science and Technology
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• v.29 no.2
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• pp.85-93
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• 2016

This study investigated the possibility of DNA contamination during fingerprint collection when using a fingerprint brush. Two kinds of brushes were selected: powdered brushes and neat (not powdered) brushes. The fingerprints were collected from the tips of all the fingers and near the wrists of both living and deceased persons using the two brushes. Both brushes were analyzed for the DNA contents and profiles. The results obtained confirmed the transfer of DNA onto both brushes, although the results showed that the powdered brushes carried more DNA compared with the neat brushes. More DNA was transferred onto the brushes used on deceased persons than onto the brushes used for living persons. Only partial DNA profiles were obtained from the brushes, which is due to the presence of other sources of DNA on the surfaces of the skin of both living and deceased persons. This phenomenon confirmed the DNA contamination during fingerprint collection when fingerprint brushes were used.

• Korean Journal of Construction Engineering and Management
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• v.19 no.2
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• pp.96-106
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• 2018

The government adopted activation policy of real estate to overcome low economic growth rate. Real estate activation plan adopted by the government raised credit limit by lowering the regulation, and reduced real estate investment cost by reducing the base rate. Also, delayed transfer tax on multi-house owner to activate real estate investment and resolved purchase right resale. Relief of real estate regulate caused increase of housing sales and price increase, and the real estate market changed to overheating aspect such as premium upon completion of lot sale in a short time. Such market atmosphere greatly increased household debs as owners own houses based on 'financial debt' instead of their income. Since 2017, real estate policy was reinforced to reduce household debts and lending rate was raised due to rise of base rate, accordingly, burden of household debt is expected to increase. This research suggested a plan for the Financial Supervisory Service to efficiently manage the financial world by analyzing the cause and problem of household debs.

• Ocean and Polar Research
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• v.31 no.4
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• pp.349-357
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• 2009

Ballast water is widely recognized as a serious environmental problem due to the risk of introducing non-indigenous aquatic species. In this study we aimed to investigate measures which can minimize the transfer of aquatic organisms from ballast water. Securing more reliable technologies to determine the viability of aquatic organisms is an important initiative in ballast water management systems. To evaluate the viability of marine phytoplankton, we designed the staining methods of fluorescein diacetate (FDA) and Calcein-AM assay on each target species belonging to different groups, such as bacillariphyceae, dinophyceae, raphidophyceae, chrysophyceae, haptophyceae and chlorophyceae. The FDA method, which is based on measurements of cell esterase activity using a fluorimetric stain, was the best dye for determining live cells of almost all phytoplankton species, except several diatoms tested in this study. On the other hand, although fluorescence of Calcein-AM was very clear for a comparatively longer time, green fluorescence per cell volume was lacking in most of the tested species. According to the Flow CAM method, which is a continuous imaging technique designed to characterize particles, green fluorescence values of stained cells by FDA were significantly higher than those of Calcein-AM treatments and control, implying that the Flow CAM using FDA assay could be adapted as an important tool for distinguishing living cells from dead cells. Our results suggest that the FDA and Calcein-AM methods can be adapted for use on phytoplankton, though species-specific characters are greatly different from one organism to another.

• Korean Journal of Veterinary Service
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• v.30 no.2
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• pp.191-196
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• 2007

The slaughter process for cattle will inevitably transfer some bacteria onto the carcasses. The goal of food safety programs is to minimize and effectively remove this contamination. This study was attempted by the Verif $EYE^{TM}$ machine-vision technology that might be useful for reducing microbial indicator counts and could reduce the contamination chance of E coli O157:H7 and Salmonella spp on beef carcasses. For the evaluation of the effectiveness of the Verif $EYE^{TM}$ technology, 80 samples were examined by the inspection device over 15 days. On an examination of FDS-positive samples compared to negative controls from the same carcasses, aerobic plate counts were bigger than the negative control samples (5.26 vs 4.60 log). Enterobacteriaceae counts were greater on the positive samples than the corresponding negative control samples (2.07 vs 1.17log). There was a consistent correlation between samples detected by the Verif $EYE^{TM}$ system with detectable counts. For example, 100% of positive samples had detectable APC and 91.2% of positive samples had detectable TCC. Therefore, if areas detected as positive for contamination by the Verif $EYE^{TM}$ system were removed from the carcasses, significant sources of microbial contamination will be reduced for objective compliance with HACCP. This results suggest that the use of Verif $EYE^{TM}$ machine-vision technology might be useful for reducing microbial indicator counts (APC, TCC) and could help reduce the risk of presence of E coJi O157:H7 and Salmonella spp on Beef carcasses.

• Journal of Dental Anesthesia and Pain Medicine
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• v.17 no.3
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• pp.163-181
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• 2017

In the field of orofacial surgery, a red blood cell transfusion (RBCT) is occasionally required during double jaw and oral cancer surgery. However, the question remains whether the effect of RBCT during the perioperative period is beneficial or harmful. The answer to this question remains challenging. In the field of orofacial surgery, transfusion is performed for the purpose of oxygen transfer to hypoxic tissues and plasma volume expansion when there is bleeding. However, there are various risks, such as infectious complications (viral and bacterial), transfusion-related acute lung injury, ABO and non-ABO associated hemolytic transfusion reactions, febrile non-hemolytic transfusion reactions, transfusion associated graft-versus-host disease, transfusion associated circulatory overload, and hypersensitivity transfusion reaction including anaphylaxis and transfusion-related immune-modulation. Many studies and guidelines have suggested RBCT is considered when hemoglobin levels recorded are 7 g/dL for general patients and 8-9 g/dL for patients with cardiovascular disease or hemodynamically unstable patients. However, RBCT is occasionally an essential treatment during surgeries and it is often required in emergency cases. We need to comprehensively consider postoperative bleeding, different clinical situations, the level of intra- and postoperative patient monitoring, and various problems that may arise from a transfusion, in the perspective of patient safety. Since orofacial surgery has an especially high risk of bleeding due to the complex structures involved and the extensive vascular distribution, measures to prevent bleeding should be taken and the conditions for a transfusion should be optimized and appropriate in order to promote patient safety.

• KIEAE Journal
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• v.12 no.3
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• pp.41-46
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• 2012

The building envelope is important specially for saving energy consumption of residential buildings. but Apartment houses in Korea commonly have inside insulation system which have constantly arisen thermal bridges, the risk of heat loss, as a necessity. This study aims to evaluate integrated insulation performance according to the different shapes of external walls, adjacent to windows. The thermal performance analysis was carried out by Equivalent U-value and using the three-dimensional heat transfer computer simulation (TRISCO-RADCON), under nine different cases of comparing among three each of different bases(current standard model, 30percent energy saving model and 60percent energy saving model). The heating and the cooling load were also compared between two cases (standard U-value and Equivalent U-value) of three each of different bases, using the Building energy simulation which is based on DOE-2.1 analysis. As results, it turns out that if the Equivalent U-value is considered on the envelope analysis, the heat flow loss will be increasing more than the standard U-value, and if heat insulation property of the residential building reinforced rather than current, the rate of influences on the thermal bridges would be extremely expanded. In addition, it is shown that annual heating loads of the apartment house with applied Equivalent U-value substantially increased by more than 15 percent compared to those with the existing U-value, but annual cooling loads were negligibly affected.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.05a
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• pp.627-628
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• 2022

There is no internationally accepted codified definition of digital trade because of the wide variety and scope of related industries and transactions(product + service + data) in general. Recently, innovative changes are taking place in digital trade due to the development of technologies such as IT due to the 4th industrial revolution, and advanced countries such as the US, EU, and Japan are including digital trade issues such as data movement liberalization in the negotiation agenda of the digital trade agreement. The issue with the liberalization of cross-border data movement is that freedom of data movement is necessary to vitalize digital trade, but it also increases the risk of information security and privacy violations. Looking at the directions of advanced countries, the US favors minimization of regulations, Europe favors regional single marketization, but passively opens up to the outside world, and China promotes independent markets through regulations. Therefore, measures to strengthen restrictions on cross-border data movement are an issue that has recently been implemented by each country or an international aggrement is scheduled to be reached soon, and Korea also needs a close response.