• The Journal of Natural Sciences
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• v.18 no.1
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• pp.47-53
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• 2007

In order to find novel sRNA in Bacillus subtilis which would be used to adapt to several conditions, we searched the whole genome of Bacillus subtilis using the following procedure. At first, the locations of recognition sequence of transcription factors such as PerR, OhrR, Fur and Zur were searched in the intergenic region of Bacillus subtilis genome and the locations of rho independent transcription terminator sites were also determined. Based on the information of these locations, the sRNA candidates were chosen by close locations (less than 300 bp) between the recognition site of transcription factors and rho independent transcription terminator site. Than transcription promoter sites were searched in the region of previously identified sRNA candidates and 5 PerR, 1 OhrR, 1 Fur and 1 Zur regulated good sRNA candidates were found.

• Journal of IKEEE
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• v.23 no.1
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• pp.249-253
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• 2019

Most of music-transcription systems that have been commercialized operate based on audio information. However, these conventional systems have disadvantages of environmental dependency, equipment dependency, and time latency. This paper studied a vision-based music-transcription system that utilizes video information rather than audio information, which is a traditional method of music-transcription programs. Computer vision technology is widely used as a field for analyzing and applying information from equipment such as cameras. In this paper, we created a program to generate MIDI file which is electronic music notes by using smart-phone cameras to record the play of piano.

• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.631-638
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• 2008

Tandem affinity purification (TAP) method combined with LC-MS/MS is the most accurate and reliable way to study the interaction of proteins or proteomics in a genome-wide scale. For the first time, we used a TAP-tag as a mutagenic tool to disrupt protein interactions at the specific site. Although lots of commonly used mutational tools exist to study functions of a gene, such as deletional mutations and site-directed mutagenesis, each method has its own demerit. To test the usefulness of a TAP-tag as a mutagenic tool, we applied a TAP-tag to RNA polymerase II, which is the key enzyme of gene expression and is controlled by hundreds of transcription factors even to transcribe a gene. Our experiment is based on the hypothesis that there will be interrupted interactions between Pol II and transcription factors owing to the TAP-tag attached at the C-terminus of each subunit of Pol II, and the abnormality caused by interrupted protein interactions can be observed by measuring a cell-cycle of each yeast strain. From ten different TAP-tagged strains, Rpb7- and Rpb12-TAP-tagged strains show severe defects in growth rate and morphology. Without a heterodimer of Rpb4/Rpb7, only the ten subunits Pol II can conduct transcription normally, and there is no previously known function of Rpb7. The observed defect of the Rpb7-TAP-tagged strain shows that Rpb7 forms a complex with other proteins or compounds and the interruption of the interaction can interfere with the normal cell cycle and morphology of the cell and nucleus. This is a novel attempt to use a TAP-tag as a proteomic tool to study protein interactions.

• BMB Reports
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• v.29 no.3
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• pp.221-224
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• 1996

To examine the growth-phase dependent control of Escherichia coli rnpB gene we used a combination of Northern analysis for RNA determination and Southern analysis for plasmid DNA determination. The relative amounts of metabolically unstable transcript derived from the internally deleted rnpB gene harbored on a multicopy plasmid as well as the relative plasmid contents were measured by Northern analysis and Southern analysis, respectively, of total nucleic acids from E coli cells containing the plasmid. The relative transcription activity of the rnB was represented by a ratio of the relative amount of the transcript to that of the plasmid DNA during bacterial growth. The rnpB transcription increased rapidly with time during exponential growth, but started to decrease before the transition period of an exponential growing cell culture into the stationary phase. Although the expression pattern was similar to the changes of ${\beta}-galactosidase$ activity expressed from the lysogenic strain carrying the chromosomal rnpB-lacZ fusion which were shown in a previous work, the present data appears to represent a more actual growth-phase control of the rnpB transcription than the previous data by the ${\beta}-galactosidase$ assay. In addition the present method described for a direct analysis of both RNA and plasmid DNA provides a rapid and efficient method that can applied to an examination of transcription control by using a multicopy plasmid.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.5
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• pp.1218-1224
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• 2008

Located on the upstream of a gene, the promoter region that plays a very important role in the control of gene expression as a signal part has various binding sites for transcription factors. These binding sites are present in various parts of the promoter region and assume an aspect of highly conserved consensus sequence pattern. This paper deals with the introductions of search methods for consensus pattern, including Wataru method, EM algorithm, MEME algorithm, Genetic algorithm and Phylogenetic Footprinting method, and intends to give future prospects of research on this field.

• Genomics & Informatics
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• v.18 no.3
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• pp.26.1-26.6
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• 2020

Single-cell RNA sequencing (scRNA-seq) has been widely applied to provide insights into the cell-by-cell expression difference in a given bulk sample. Accordingly, numerous analysis methods have been developed. As it involves simultaneous analyses of many cell and genes, efficiency of the methods is crucial. The conventional cell type annotation method is laborious and subjective. Here we propose a semi-automatic method that calculates a normalized score for each cell type based on user-supplied cell type-specific marker gene list. The method was applied to a publicly available scRNA-seq data of mouse cardiac non-myocyte cell pool. Annotating the 35 t-stochastic neighbor embedding clusters into 12 cell types was straightforward, and its accuracy was evaluated by constructing co-expression network for each cell type. Gene Ontology analysis was congruent with the annotated cell type and the corollary regulatory network analysis showed upstream transcription factors that have well supported literature evidences. The source code is available as an R script upon request.

• Journal of architectural history
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• v.17 no.3
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• pp.45-60
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• 2008

This study lists the vocabulary of the inscriptions on SeoGwol-YeongGeon-DoGam-UiGwe(西闕營建都監儀軌 1831). This study also deciphers and explains the meanings of them. In advance, this study compares them with the vocabulary of the national language in the middle ages and in modem times. There are two important missions in deciphering a transcription marking of the architectural vocabulary of UiGwe(儀軌). One is to gain an understanding of the reading method of transcription marking and the other is an explanation of what that means. As a result, we can correctly understand the UiGwe(儀軌) written in transcription marks. If we could decipher the transcription markings of the documents as it is, we cannot only recover plenty of vocabulary related with characteristic architecture in the age of the later Joseon Dynasty, but also correct wrongly used vocabulary in the present. As a result, we can standardize and adjust the vocabulary use of Korean traditional architecture. In advance, we can correct errors of spelling and mistaken explanations in the Korean Encyclopedia.

• Research in Plant Disease
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• v.27 no.2
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• pp.79-83
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• 2021

The aim of the present study was to develop a sensitive and specific detection method for the rapid detection of apple scar skin viroid (ASSVd) in apple leaves. The resulting reverse transcription recombinase polymerase amplification (RT-RPA) assay can be completed in 10 min at 42℃, is 10 times more sensitive than conventional reverse transcription polymerase chain reaction, and can specifically amplify ASSVd without any cross-reactivity with other common apple viruses, including apple stem grooving virus, apple stem pitting virus, and apple chlorotic leaf spot virus. The reliability of the RT-RPA assay was assessed, and the findings suggested that it can be successfully utilized to detect ASSVd in field-collected samples. The RT-RPA assay developed in the present study provides a potentially valuable means for improving the detection of ASSVd in viroid-free certification programs, especially in resource-limited conditions.

• Speech Sciences
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• v.9 no.1
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• pp.111-135
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• 2002

An annotation model of a non-native speech database has been devised, wherein English is the target language and Korean is the native language. The proposed annotation model features overt transcription of predictable linguistic information in native speech by the dictionary entry and several predefined types of error specification found in native language transfer. The proposed model is, in that sense, different from other previously explored annotation models in the literature, most of which are based on native speech. The validity of the newly proposed model is revealed in its consistent annotation of 1) salient linguistic features of English, 2) contrastive linguistic features of English and Korean, 3) actual errors reported in the literature, and 4) the newly collected data in this study. The annotation method in this model adopts the widely accepted conventions, Speech Assessment Methods Phonetic Alphabet (SAMPA) and the TOnes and Break Indices (ToBI). In the proposed annotation model, SAMPA is exclusively employed for segmental transcription and ToBI for prosodic transcription. The annotation of non-native speech is used to assess speaking ability for English as Foreign Language (EFL) learners.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1997.04a
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• pp.123-126
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• 1997

In this study, we investigated the Pretilt ang1e generation in nematic liquid crystal (NLC) by using the transcription, alignment on polyimide(Pl) surfaces. That the uniform alignment of NLC is observed by microphotograph textures in LC cells on Pl surface. We obtained that the pretilt angle of NLC are generated about 3.6$^{\circ}$on Pl surface. It is considered that the LC alignment by transcription alignment method is attributed to memory effect of LC on Pl surface.