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http://dx.doi.org/10.5423/RPD.2021.27.2.79

Detection of Apple Scar Skin Viroid by Reverse Transcription Recombinase Polymerase Amplification Assay  

Kim, Na-Kyeong (Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University)
Lee, Hyo-Jeong (Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University)
Ryu, Tae-Ho (Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University)
Cho, In-Sook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Ju, Ho-Jong (Department of Agricultural Biology, College of Agriculture & Life Sciences, Jeonbuk National University)
Jeong, Rae-Dong (Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University)
Publication Information
Research in Plant Disease / v.27, no.2, 2021 , pp. 79-83 More about this Journal
Abstract
The aim of the present study was to develop a sensitive and specific detection method for the rapid detection of apple scar skin viroid (ASSVd) in apple leaves. The resulting reverse transcription recombinase polymerase amplification (RT-RPA) assay can be completed in 10 min at 42℃, is 10 times more sensitive than conventional reverse transcription polymerase chain reaction, and can specifically amplify ASSVd without any cross-reactivity with other common apple viruses, including apple stem grooving virus, apple stem pitting virus, and apple chlorotic leaf spot virus. The reliability of the RT-RPA assay was assessed, and the findings suggested that it can be successfully utilized to detect ASSVd in field-collected samples. The RT-RPA assay developed in the present study provides a potentially valuable means for improving the detection of ASSVd in viroid-free certification programs, especially in resource-limited conditions.
Keywords
Apple leaves; Apple scar skin viroid; Detection; Reverse transcription recombinase polymerase amplification;
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