• 한국지능시스템학회논문지
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• 제17권5호
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• pp.696-700
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• 2007

본 논문에서는 화자 독립 음소 인식기를 사용하는 음소열 기반 음성 인식 시스템의 성능을 향상시키는 새로운 방법을 제안하였다. 화자독립 음소 HMM을 사용하는 음성 인식 시스템은 입력 문장에 대한 음소열만을 사용하므로 저장 공간은 크게 줄일 수 있다. 그러나 시스템의 성능은 화자독립 모델을 사용하므로 발생하는 음소 오차 때문에 화자 종속 시스템보다 저하된다. 여기에서는 화자 적응 기술을 사용하여 화자독립 모델과 학습 데이터간의 불일치를 감소시키도록 음소열과 변환 벡터를 반복적으로 추정하는 학습 방법을 제안하였다. 화자 적응을 위한 변환 벡터를 추정하기 위하여 확률적 매칭 방법이 사용되었다. 실험은 전화선을 통하여 얻어진 데이터를 사용한 실험에서 기존 방법에 비하여 약 45%정도 오차가 감소되었다.

• 대한화학회지
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• 제52권4호
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• pp.394-403
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• 2008

• Archives of Pharmacal Research
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• 제19권6호
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• pp.486-489
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• 1996

A simple, rapid, specific and sensitive method for the detection of serum hepatitis C virus (HCV) RNA using the reverse transcription-polymerase chain reaction (RT-PCR) technique without conventional RNA extraction was developed. HCV template RNA from serum was obtained by boiling the serum at $95^{\circ}C$ for 2 min, cooling rapidly in ice and removing the proteins by cetrifugation. RT-PCR amplifications including the reverse transcription and first PCR amplification were performed in one vessel containing both of reverse transcriptase and Taq DNA polymerase. The detection of HCV RNA from $10^{-3}{\mu}l$. serum was possible with this method. The suitability of this method for clinical analysis was evaluated by assaying HCV RNA in 225 patient samples including anti-HCV antibody negatives (13 samples) and positives (212 samples) by enzyme-linked immunosorbent assay test (ELISA). Detections of HCV RNA with this method were in 4 of 13 anti-HCV antibody negative samples (30.8%) and 95 of 212 positive samples (44.8%). The present method can be completed in 1 hr and has a wide range of application for the clinical utilities to determine the viral RNAS.

• The Plant Pathology Journal
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• 제36권5호
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• pp.497-502
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• 2020

Barley yellow dwarf virus (BYDV) is an economically important plant pathogen that causes stunted growth, delayed heading, leaf yellowing, and purple leaf tip, thereby reducing the yields of cereal crops worldwide. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed for the detection of BYDV in oat leaf samples. The RT-RPA assay involved incubation at an isothermal temperature (42℃) and could be performed rapidly in 5 min. In addition, no cross-reactivity was observed to occur with other cereal-infecting viruses, and the method was 100 times more sensitive than conventional reverse transcription polymerase chain reaction. Furthermore, the assay was validated for the detection of BYDV in both field-collected oat leaves and viruliferous aphids. Thus, the RT-RPA assay developed in the present study represents a simple, rapid, sensitive, and reliable method for detecting BYDV in oats.

• The Plant Pathology Journal
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• 제38권2호
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• pp.159-166
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• 2022

Barley yellow dwarf virus (BYDV) has been a major viral pathogen causing significant losses of cereal crops including oats worldwide. It spreads naturally through aphids, and a rapid, specific, and reliable diagnostic method is imperative for disease monitoring and management. Here, we established a rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat samples based on the conserved sequences of the BYDV coat protein gene. Specific primers and a probe for RT-RPA reacted and optimally incubated at 42℃ for 10 min, and the end-labeled amplification products were visualized on LFS within 10 min. The RT-RPA-LFS assay showed no cross-reactivity with other major cereal viruses, including barley mild mosaic virus, barley yellow mosaic virus, and rice black streaked dwarf virus, indicating high specificity of the assay. The sensitivity of the RT-RPA-LFS assay was similar to that of reverse transcription polymerase chain reaction, and it was successfully validated to detect BYDV in oat samples from six different regions and in individual aphids. These results confirm the outstanding potential of the RT-RPA-LFS assay for rapid detection of BYDV.

• 한국음향학회지
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• 제29권2호
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• pp.102-110
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• 2010

악보 전사란, 오디오 파일로부터 음고 (음표의 높낮이)와 리듬 (음표의 길이) 정보를 추출하여 악보를 만드는 것이다. 본 논문에서는 음원 분리 및 데이터 분류에 자주 사용되는 Non-Negative Matrix Factorization (NMF)와 Non-Negative Sparse Coding (NNSC) 방식을 사용하여 오디오 파일을 주파수와 리듬 성분으로 분류하였다. 또한 배음 통합 (subharmonic summation) 방법으로 분류된 주파수들로부터 기본 진동 주파수를 계산하였고, 이로써 악보를 야루는 음표의 높낮이를 정확히 얻을 수 있었다. 제안한 방식으로 악보 전사거 성공적으로 이루어졌고, NMF 혹은 NNSC만 사용하여 악보 전사를 하였던 기존의 논문들에 비해 향상된 결과를 얻을 수 있었다.

• The Plant Pathology Journal
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• 제27권4호
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• pp.385-389
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• 2011

A new reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the Potato leafroll virus (PLRV) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to address its advantages over RTPCR. RT-LAMP primers were designed from the open reading frame 3 (ORF3) sequence of PLRV. The RT-LAMP reactions were conducted without or with a set of loop primers. By real-time monitoring using Turbimeter, the RT-LAMP (with loop primers) detects PLRV in less than 30 min, compared to 120 min of RT-PCR. By adding fluorescent reagent during the reaction, final products of the RT-LAMP were fluorescently visualized under UV light or could be differentiated by naked-eye inspection under normal light. The RT-LAMP was extremely sensitive, about 2000-fold more sensitive than RT-PCR. This study presents great potential of the RT-LAMP for diagnosis and PLRV epidemiology because RT-LAMP method is speedy, sensitive, inexpensive, and convenient.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1997년도 하계학술대회 논문집 C
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• pp.1582-1584
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• 1997

We investigated the transcription of liquid crystal (LC) alignment method by using memory effect of nematic (N) LC on polyimide (PI) surface with side chain as for non-rubbing alignment techniques. That the monodomain alignment of aligned NLC is observed by polarizing microscope textures in the cells on PI surface with side chain, We obtained that the pretilt angle of NLC are generated about 3.7 degree on PI surface. We suggest that the LC alignment by using transcription aligngnment method is attributed to memory effect of NLC on PI surface.

• 통합자연과학논문집
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• 제5권3호
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• pp.163-167
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• 2012

UV spectrophotometer was used to detect protein-DNA complex from DNA melting profile under constant temperature increase. Melting temperature (Tm) was $43^{\circ}C$ in copA duplex DNA alone. In the presence of Proteus mirabilis transcription regulator protein (PMTR) protein at 0.2 and 0.4 ${\mu}M$, Tm's were $45{\pm}0.5$ and $47.6{\pm}0.6^{\circ}C$, respectively. According to fluorescence polarization and gel shift assay. PMTR:copA complex was detected by the retarded migration on gel and the dissociation constant ($K_d$) was $(9.2{\pm}2.8){\times}10^{-9}M$.

• Journal of Microbiology and Biotechnology
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• 제19권11호
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• pp.1470-1474
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• 2009

This paper describes the development a of direct multiplex reverse transcription-nested polymerase chain reaction (PCR) method, devised for simultaneous detection and typing of influenza viruses. This method combines the direct reverse transcription reaction without RNA purification with the enhancement of sensitivity and specificity of nested PCR. The method successfully detected three major human influenza viruses: influenza virus A subtype 1 (H1N1) and subtype 3 (H3N2), and influenza B virus (B). The minimum number of virus particles (pfu/ml) necessary for detection in spiked saliva samples was 200 (H1N1), 140 (H3N2), and 4.5 (B). The method's sensitivity and simplicity will be convenient for use in clinical laboratories for the detection and subtyping of influenza and possibly other RNA viruses.