DOI QR코드

DOI QR Code

Complex Detection Between Transcription Regulator and Promoter DNA by UV Spectroscopic Method

  • Received : 2012.08.29
  • Accepted : 2012.09.25
  • Published : 2012.09.30

Abstract

UV spectrophotometer was used to detect protein-DNA complex from DNA melting profile under constant temperature increase. Melting temperature (Tm) was $43^{\circ}C$ in copA duplex DNA alone. In the presence of Proteus mirabilis transcription regulator protein (PMTR) protein at 0.2 and 0.4 ${\mu}M$, Tm's were $45{\pm}0.5$ and $47.6{\pm}0.6^{\circ}C$, respectively. According to fluorescence polarization and gel shift assay. PMTR:copA complex was detected by the retarded migration on gel and the dissociation constant ($K_d$) was $(9.2{\pm}2.8){\times}10^{-9}M$.

Keywords

References

  1. M. J. Guille and G. G. Kneale, "Methods for the analysis of DNA-protein interaction", Mol. Biotechnol., Vol. 8. pp. 35-52, 1997. https://doi.org/10.1007/BF02762338
  2. M. L. Carpenter and G. G. Kneale, "Circular dichro-ism and fluorescence analysis of the interaction of Pf1 gene 5 protein with poly(dT)", J. Mol. Biol., Vol. 217, pp. 681-689, 1991. https://doi.org/10.1016/0022-2836(91)90525-B
  3. M. G. Fried and D. M. Crothers, "Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis", Nucleic Acids Res., Vol. 9, pp. 6505-6525. 1981. https://doi.org/10.1093/nar/9.23.6505
  4. A. Schmitz and D. J. Galas, "DNase I footprinting: a simple method for the detection of protein-DNA binding specificity", Nucleic Acids Res., Vol. 5, pp. 3157-3170, 1978. https://doi.org/10.1093/nar/5.9.3157
  5. J. Welsh and C. R. Cantor. "Protein-DNA crosslinking", Trends Biochem. Sci., Vol. 9, pp. 505-507, 1984. https://doi.org/10.1016/0968-0004(84)90271-8
  6. H. Singh, J. H. LeBowitz, A. S. Baldwin, and P. A. Sharp, "Molecular cloning of an enhancer binding protein: isolation by screening an expression library with recognition site DNA" Cell, Vol. 52, pp. 415- 423, 1988. https://doi.org/10.1016/S0092-8674(88)80034-5
  7. M. Noll, K. Petrukhin, and S. Lutchenko, "Identification of a novel transcription regulator from Proteus mirabilis, PMTR, revealed a possible role of YJAI protein in balancing zinc in E. coli", J. Biol. Chem., Vol. 273, pp. 21393-21401, 1998. https://doi.org/10.1074/jbc.273.33.21393
  8. E. A. Permina, A. E. Kazakov, O. V. Kalininia, and M. S. Gelfand, "Comparative genomics of regulation of heavy metal resistance in Eubacteria", BMC Microbiol., Vol. 6. pp. 1-11, 2006. https://doi.org/10.1186/1471-2180-6-1
  9. D. H. Nies, "Microbial heavy-metal resistance", Appl. Microbiol. Biotechnol., Vol. 51. pp. 730-750, 1999. https://doi.org/10.1007/s002530051457
  10. A. Changela, K. Chen, Y. Xue, J. Holschen, C. E. Outten, T. V. O'Halloran, and A. Mondragon, "Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR' Science, Vol. 301. pp. 1383-1387, 2003. https://doi.org/10.1126/science.1085950
  11. F. W. Outten, C. E. Outten, J. Hale, and T. V. O'Halloran, "Transcription activation of an Escherichia coli copper efflux regulon by the chromosomal MerR homologue, CueR", J. Biol. Chem., Vol. 275, pp. 31024-31029, 2000. https://doi.org/10.1074/jbc.M006508200
  12. D. Dimova, P. Weigel, M. Takahashi, F. Marc, G. D. Van Duyne, and V. Sakanyan, "Thermostability, oligomerizatin and DNA-binding properties of the regulatory protein ArgR from the hyperthermophilic bacterium thermotoga neapolitana", Mol. Gen. Genet., Vol. 263, pp. 119-130. 2000.
  13. J. R. Lunblad, M. Laurance, and R. H. Goodman, "Fluorescence polarization analysis of protein-DNA and protein-protein interaction", Mol. Endocrinol. Vol. 10, pp. 607-612, 1996. https://doi.org/10.1210/me.10.6.607