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http://dx.doi.org/10.5423/PPJ.NT.08.2020.0148

Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid and Sensitive Detection of Barley Yellow Dwarf Virus in Oat  

Kim, Na-Kyeong (Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University)
Kim, Sang-Min (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration)
Jeong, Rae-Dong (Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University)
Publication Information
The Plant Pathology Journal / v.36, no.5, 2020 , pp. 497-502 More about this Journal
Abstract
Barley yellow dwarf virus (BYDV) is an economically important plant pathogen that causes stunted growth, delayed heading, leaf yellowing, and purple leaf tip, thereby reducing the yields of cereal crops worldwide. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed for the detection of BYDV in oat leaf samples. The RT-RPA assay involved incubation at an isothermal temperature (42℃) and could be performed rapidly in 5 min. In addition, no cross-reactivity was observed to occur with other cereal-infecting viruses, and the method was 100 times more sensitive than conventional reverse transcription polymerase chain reaction. Furthermore, the assay was validated for the detection of BYDV in both field-collected oat leaves and viruliferous aphids. Thus, the RT-RPA assay developed in the present study represents a simple, rapid, sensitive, and reliable method for detecting BYDV in oats.
Keywords
barley yellow dwarf virus; rapid detection; reverse transcription recombinase polymerase amplification;
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