• Title/Summary/Keyword: Tradescantia-micronucleus (Trad-MCN)

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Genotoxicity of Heavy Metals among the Particulates in the Working Environment as Assessed by Tradescantia-Micronucleus (Trad-MCN) Assay (자주달개비 미세핵 분석법을 이용한 작업환경내 총 먼지 중 수용성 추출물의 유전독성 평가)

  • Shin, Hae-Shik;Kim, Jin-Kyu;Lee, Jae-Hwan;Hwang, Kap-Sung;Kim, Kyun;Lee, Jeong-Joo;Lee, Jin-Hong
    • Journal of Korean Society for Atmospheric Environment
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    • v.19 no.6
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    • pp.639-646
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    • 2003
  • In this study, we evaluated genotoxicity of heavy metals among particulates with Tradescantia-micronucleus (Trad-MCN) assay in the various working environment. In a synthetic fiber factory and a rubber factory, chromium concentration was higher than any other heavy metals. On the other hand, nickel concentration was the highest in a semi-conductor factory. The difference in genotoxicity among the working environment was statistically significant as Trad-MCN frequencies were 4.07 $\pm$0.35 MCN/100 tetrads (p< 0.01) for the synthetic fiber factory,5.73 $\pm$0.81 MCN/100 tetrads (p< 0.01) for the rubber factory, and 15.60$\pm$2.58 (p< 0.01) (p< 0.001) for the semi -conductor factory. As a result, heavy metals among particulates in the working environment can be considered to have hazardous potential to human health, although they cannot directly induce DNA damage to the workers in the working environments.

The Comparison of Volatile Organic Compounds (VOCs) Analysis and Tradescantia Micronucleus (Trad-MCN) Bioassay for Evaluation of Hazardous Materials in Chemical Workplace Field (화학공장 실내 작업장에서의 유해물질 평가를 위한 VOC 분석법과 자주달개비 미세핵 분석법의 비교)

  • Heo, Gwi Suk;Lee, Jae Hwan;Shin, Hae Shik;Kim, Jin Kyu;Lee, Young Yup;Lee, Dai Woon;Lee, Jin Hong
    • Analytical Science and Technology
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    • v.16 no.1
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    • pp.1-11
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    • 2003
  • This research examined the presence of hazardous materials in chemical workplace field using an integrated chemical/biological monitoring. Chemical workplace field air for volatile organic compounds (VOCs) analysis was collected using a collection tube packed with Tena.x TA adsorbent 400 mg. Workplace field air samples were analyzed by gas chromatography/mass spectrometry (GC/MS). Simultaneously, Tradescantia BNL 4430 clone was exposed in situ to monitor hazardous materials in chemical workplace field. GC/MS analysis showed the presence of various VOCs such as trichloroethylene, toluene, ethylbenzene, (m,p,o)-xylenes, styrene, 1,3,5-trimethylbenzene, and 1,2,4-trimethylbenzene. The results showed that in situ monitoring of VOCs with the Tradescantia-micronucleus (Trad-MCN) assay gave positive results in chemical workplace field and negative response at outdoor air. In conclusion, inhalation of these field air by workers may affect chronic demage to their health by inducing micronuclei formation in Tradescantia pollen mother cells. The combination of chemical/biological monitoring is very effective to evaluate hazardous materials in workplace field and can be alternatively used for screening hazardous materials.