• Archives of Pharmacal Research
• /
• 제20권1호
• /
• pp.46-52
• /
• 1997

To obtain more sensitive immunoassay for methamphetamine (MA) determination, the optimum condition of enzyme-linked immunosorbent assay (ELISA) was investigated in regard to immunogens, antibody purification methods and coating tracers. Activated MA, N-(4-aminobutyl)methamphetamine (4-ABMA), was conjugated with bovine serum albumin (BSA) or keyhole limpet hemocyanin (KLH) and used as immunogen. The antibodies were purified by protein G chromatography or various immunoaffinity chromatography-linked MA-protein ligands, such as MA-BSA, MA-KLH or MA-ovalbumin (OVA). Each purified antibody was characterized by means of sensitivity and cross-reactivity using the three MA-protein coating tracers, MA-BSA, MA-KLH and MA-OVA. The best sensitivity of each antibody was acquired with the MA-OVA tracer although the tracer concentration and the antibody titer level at optimum condition were varied. The antibody with high titer level did not always yield good sensitivity. At optimum condition, immunoaffinity chromatography-purified antibodies were better for sensitivity and for specificity than protein G-purified antibodies. The cross-reactivity of the purified antibodies seemed to be affected by immunogen structure and showed somewhat different patterns according to the immunoaffinity ligand utilized. These data show that the antibody purification method as well as choice of coating tracer and immunogen is essential for the sensitivity and specificity of EIA; the optimum condition for assay should be discovered using various methods and combinations.

• 한국수처리학회지
• /
• 제26권6호
• /
• pp.3-12
• /
• 2018

The objective of this study was to evaluate the radius of influence of effluent of water treatment system developed for the purpose of improvement of reservoir water quality using fluorescent dye (Rhodamine-WT) tracer experiment and 3-D numerical model. The tracer experiment was carried out in a medium-sized agricultural reservoir with a storage capacity of $227,000m^3$ and an average depth of 1.6 m. A guideline with a total length of 160 m was installed at intervals of 10 m in the horizontal direction from the discharge part, and a Rhodamine measurement sensor (YSI 6130, measurement range $0-200{\mu}g/L$) was used to measure concentration changes in time, distance, and depth. Experimental design was established in advance through Jet theory and the diffusion process was simulated using ELCOM, a three dimensional hydraulic dynamics model. As a result of the study, the direct effect radius of the jet emitted from the applied water treatment system was about 50-70 m, and the radius of physical effect by the advection diffusion was judged to be 100-120 m. The numerical simulations of effluent advection-diffusion of the water treatment system using ELCOM showed very similar results to those of the impact radius analysis using the tracer experiment and jet flow empirical equations. The results provide valuable information on the spatial extent of the water quality improvement devices installed in the reservoir and the facility layout design.

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제12권4호
• /
• pp.86-93
• /
• 2007

최근에 형광물질을 이용한 추적자 시험이 빈번해지고 있다. 본 연구에서는 형광물질 추적자 시험시 농도측정 방법으로서 입상 활성탄을 이용하는 방법의 적용가능성을 실내 및 현장실험을 통해 평가하였다. 실내실험 결과 로다민 WT 및 우라닌 개별용액의 경우 활성탄 흡착 농도는 실제 표준농도와는 일부 차이가 있지만 각 표준농도와 선형성은 매우 뛰어난 것으로 나타났다. 또한 $10{\mu}g/L$ 이상의 형광물질 농도에서는 활성탄의 적용성이 뛰어났다. 한편 두 형광물질을 혼합한 경우에는 간섭의 영향으로 활성탄 적용성이 떨어졌다. 실제 현장에서 활성탄을 이용하여 추적자 시험을 한 결과, 통상적으로 수행하는 직접적인 물시료 채취분석법과 비교하였을 때 활성탄을 이용한 흡착방법도 충분히 현장 적용성이 있으며 저농도에서는 습식 그리고 고농도에서는 건식으로 분석한다면 보다 정확한 형광물질 농도측정이 되는 것으로 나타났다. 특히 오염물질 확산 평가 등이 목적이 아니라 추적자 물질의 도달확인(즉 수리적 연결성 진단)이 주요 목적이고 추적자시험의 예상 소요기간이 길다면 활성탄을 활용한 방법은 매우 비용경제적일 것으로 사료된다.

• 한국환경과학회지
• /
• 제20권5호
• /
• pp.581-587
• /
• 2011

Industrial natural ventilation systems consist of gravity ventilator, the high/low windows and doors. Especially, the high windows play an important role in the industrial natural ventilation systems. Generally speaking, industrial high windows are divided into 3 types; louver type, $45^{\circ}$ open type and $90^{\circ}$ open type. This study was numerically and experimentally conducted. Three types of windows were tested to know the ventilation characteristics and estimate the ventilation efficiencies. Numerically, computational fluid dynamics software (AIR PAK Ver. 2.0) was used to observe the flow characteristics inside the industrial building and the concentration contours generated by the tracer gas method. Experimentally, the flow visualization technique and the tracer gas method were applied with the model building to characterize the flow pattern inside the model building and to estimate the ventilation efficiencies with the different windows. It was found that $90^{\circ}$ open type window was most effective for the discharge of pollutants from the industrial building. On the other hand, the louver type window was found to be less effective than any other windows.

• 대한의생명과학회지
• /
• 제11권2호
• /
• pp.129-134
• /
• 2005

Three different deconvolution techniques for quantifying cerebral blood flow (CBF) from whole brain $T2^{\ast}-weighted$ bolus tracking images were implemented (parametric Fourier transform P-FT, parametric single value decomposition P-SVD and nonparametric single value decomposition NP-SVD). The techniques were tested on 206 regions from 38 hyperacute stroke patients. In the P-FT and P-SVD techniques, the tissue and arterial concentration time curves were fit to a gamma variate function and the resulting CBF values correlated very well $(CBF_{P-FT}\;=\;1.02{\cdot}CBF_{p-SVD},\;r^2\;=\;0.96)$. The NP-SVD CBF values correlated well with the P-FT CBF values only when a sufficient number of time series volumes were acquired to minimize tracer time curve truncation $(CBF_{P-FT}\;=\;0.92{\cdot}CBF_{NP-SVD},\;r^2\;=\;0.88)$. The correlation between the fitted CBV and the unfitted CBV values was also maximized in regions with minimal tracer time curve truncation $(CBV_{fit}\;=\;1.00{\cdot}CBV_{ Unfit},\;^r^2\;=\;0.89)$. When a sufficient number of time series volumes could not be acquired (due to scanner limitations) to avoid tracer time curve truncation, the P-FT and P-SVD techniques gave more reliable estimates of CBF than the NP-SVD technique.

• Nuclear Engineering and Technology
• /
• 제46권1호
• /
• pp.93-100
• /
• 2014

The paper deals with the application of radio isotopic non-destructive technique in the characterization of two industrial grade anion exchange resins Indion GS-300 and Indion-860. For the characterization of the two resins, $^{131}I$ and $^{82}Br$ were used as tracer isotopes to trace the kinetics of iodide and bromide ion-isotopic exchange reactions. It was observed that the values of specific reaction rate ($min^{-1}$), amount of iodide ion exchanged (mmol), initial rate of iodide ion exchange (mmol/min) and log $K_d$ were calculated as 0.328, 0.577, 0.189 and 19.7 respectively for Indion GS-300 resin, which was higher than the respective values of 0.180, 0.386, 0.070 and 17.0 calculated for Indion-860 resins when measured under identical experimental conditions. Also at a constant temperature of $40.0^{\circ}C$, as the concentration of labeled iodide ion solution increases 0.001 M to 0.004 M, the percentage of iodide ions exchanged increases from 75.16 % to 78.36 % for Indion GS-300 resins, which was higher than the increases from 49.65 % to 52.36 % compared to that obtained for Indion-860 resins. The overall results indicate that under identical experimental conditions, Indion GS-300 resins show superior performance over Indion-860 resins.

• 한국세라믹학회지
• /
• 제31권11호
• /
• pp.1369-1377
• /
• 1994

• Environmental Engineering Research
• /
• 제23권2호
• /
• pp.195-204
• /
• 2018

A pilot scale study was conducted on a down-flow hanging sponge (DHS) reactor installed at a sewage treatment plant in Banda Aceh, Indonesia for treatment of desludging septic tank wastewater. Raw wastewater with an average biochemical oxygen demand (BOD) and total suspended solids of 139 mg/L and 191 mg/L, respectively, was pumped into the reactor. Two different hydraulic retention times (HRTs, 3 h and 4 h) were investigated, equivalent to organic loadings of 1.11 and $0.78kg\;BOD/m^3/d$, respectively. The average BOD concentration in the final effluent was 46 and 26 mg/L at HRTs of 3 and 4 h, respectively. The concentration of retained sludge along the reactor height was 10.2-18.7 g VSS/L-sponge, and the sludge activities were 0.24-0.32 and 0.04-0.40 mg/g VSS/h for heterotrophs and nitrification, respectively. Values of water hold-up volume, dispersion coefficient, and number of tank in-series found from tracer studies of clean sponge and biomass-loaded sponge confirmed that growth of retained sludge on the sponge module improved hydraulic performance of the reactor. Adoption of the DHS reactor by this Indonesian sewage treatment plant would enhance the role of the current desludging septic tank wastewater treatment system.

• 대한설비공학회:학술대회논문집
• /
• 대한설비공학회 2009년도 하계학술발표대회 논문집
• /
• pp.148-153
• /
• 2009

This paper considers the age of air in a ventilated space with multiple supply inlets. The local mean age of air at a point from one supply inlet is different from those from the other supply inlets. It is the purpose of the present paper to investigate theoretically the relations between the LMA's from each supply and overall combined LMA whether or not to trace the origins of supply air. Transient concentration distributions are calculated with a step-up injection of tracer gas at each supply inlet, and at both inlets simultaneously. The steady state concentration with a continuous tracer injection at a supply inlet works as a weighting factor for the corresponding LMA in calculating the average overall LMA from multiple inlets.

• 분석과학
• /
• 제8권4호
• /
• pp.427-434
• /
• 1995

Inductively coupled plasma mass spectrometry combined with the isotope dilution method is used for the determination of lithium. The isotope dilution method is based on the addition of a known amount of enriched isotope (spike) to a sample. The analyte concentration is obtained by measuring the altered isotope ratio. The spike solution is calibrated through so called reverse isotope dilution with a primary standard. The spike calibration is an important step to minimize error in the determined concentration. It has been found essential to add spike to a sample and the primary standard so that the two isotope ratios should be as dose as possible. Since lithium is neither corrosive nor toxic, lithium is used as a chemical tracer in the nuclear power plants to measure feedwater flow rate. 99.9% $^7Li$ was injected into a feedwater line of an experimental system and sample were taken downstream to be spiked with 95% $^6Li$ for the isotope dilution measurements. Effects of uncertainties in the spike enrichment and isotope ratio measurement error at various spike-to-sample ratios are presented together with the flow rate measurement results in comparison with a vortex flow meter.