• 한국수정란이식학회지
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• 제29권3호
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• pp.283-287
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• 2014

Sexed semen is commonly used for the production of calves of the desired gender. Gender selection is important in animal production industries. For example, female cattle are required for the dairy industry while males are preferred in the beef cattle industry. The present study was to assess the in vivo embryo production efficiency using the semen separated according to sex during superovulation in Hanwoo. Seventy Hanwoo donor cows were flushed on day 7 of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered on 7 days after the third insemination by flushing the uterus with embryo collection medium. KPN semen straws used artificial insemination contained 20 million sperm (total number 60 million per donor). Sex-sorted semen straws contained 4 million sperm (total number 12 million per donor). The results obtained were as follows: No differences were observed in the efficiency of superovulation rates on KPN semen 87%, and sexed semen 100%, respectively. The mean numbers of total embryos are each $12.58{\pm}8.31$ and $13.25{\pm}7.86$. The mean numbers of transferable embryos, sexed semen were significantly lower than KPN semen ($3.75{\pm}1.98$ vs. $8.23{\pm}6.07$, P<0.05). The rates of unfertilized embryos from superovulation using sexed semen were significantly higher than KPN semen (50% vs. 15%, P<0.05). The rate of degenerated 2-cell embryos from sexed and KPN semen was 60.87% and 11.11%, respectively (p<0.05). In conclusion, these results indicate that superovulation using sexed semen was useful, but efficient embryo production was important to reducing the damage caused by the Flowcytometer-based sperm sorting procedure.

• 한국수정란이식학회지
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• 제25권3호
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• pp.145-148
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• 2010

Oxygen consumption has been regarded as a useful indicator for assessment of mammalian embryo quality. This study was performed to investigate whether oxygen consumption reflects morphological grade of in vivo derived bovine blastocyst-stage embryos (blastocyst). The oxygen consumption of in vitro produced blastocyst was compared to its total cell number. In addition, pregnant rate was measured after transplantation of in vivo blastocysts with different oxygen consumption. The quality of blastocyst collected on day 7 after artificial insemination was categorized as grade I and II (G I and G II) based on microscopic observation of the morphology. Oxygen consumption of blastocyst was measured using a scanning electrochemical microscopy (SECM) and total cell number of in vitro blastocyst was enumerated by counting cells stained by propidium iodide. Pregnancy of recipient cow was confirmed with rectal palpation after 60 days of embryo transfer. The oxygen consumptions of G I blastocysts were significantly higher than those of G II blastocysts ($10.2{\times}10^{15}/mol\;s^{-1}$ versus $6.4{\times}10^{15}/mol\;s^{-1}$, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7, and 110.2 in the oxygen consumption of below 10.0, 10.0~12.0, and over $12.0{\sim}10^{15}/mol\;s^{-1}$ respectively. Total cell number was significantly increased in embryos with high oxygen consumption (p<0.05). Pregnant rate in recipient cow was 0, 50, and 85.7% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0, and over $12.0{\times}10^{15}/mol\;s^{-1}$, respectively. These results suggest that measurement of oxygen consumption may help increase the pregnant rate of bovine embryos.

• Asian-Australasian Journal of Animal Sciences
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• 제25권3호
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• pp.320-324
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• 2012

The objective of this study was to evaluate the effect of spent mushroom substrate (SMS) derived from Pleurotus eryngii on the hematological and biochemical blood properties of elk. A total of 18, two and three-year-old elk were fed three different levels of SMS (0, 15 and 20%) in a corn-wheat bran diet for 80 days. The results indicated significantly high levels of blood monocytes, hemoglobin (Hb), and hematocrit (HCT) in elk fed 15% or 20% SMS (p<0.05) compared to control animals. Serum blood urea nitrogen (BUN) and glucose concentrations were also significantly elevated in elk fed both 15% and 20% SMS. The inclusion of SMS in the elk diet did not affect serum total cholesterol, triglyceride, or low density lipoprotein (LDL)-cholesterol concentrations; however, high density lipoprotein (HDL)-cholesterol concentration was significantly increased in SMS-fed groups. In addition, 20% SMS in the diet increased serum iron and testosterone concentrations in elk. These results indicate that adding SMS to the diet of elk can increase their Hgb, serum BUN, glucose, and HDL-cholesterol concentration; therefore, diets containing SMS may enhance the physiologic condition of elk during growth.

• 한국산학기술학회논문지
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• 제21권4호
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• pp.582-587
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• 2020

최근 기상이변으로 인한 재해가 전 세계적으로 증가하고 있으며, 세계적으로 재해로 인한 피해가 커지고 있어 국내에서도 지진, 태풍, 지반침하 등 재해에 대한 관심이 높아지고 있다. 우리나라는 재난관리에 의한 특별법을 제정하고, 장대교량 및 초고층 복합건축물은 시설물의 변위상태 측정을 위해 정밀센서와 관련 시스템 등을 구축하여 개별 시설물 단위의 모니터링 시스템을 구축하도록 하고 있다. 하지만 사면이나 노천광산, 중, 소규모의 구조물에 대해서는 기상, 측정방법, 비용, 상시 모니터링의 어려움으로 인해 실시간 모니터링 시스템의 적용이 미흡한 실정이다. 이에 본 연구에서는 토털스테이션을 활용한 변위 모니터링 방안을 연구하고, 실험을 통해 적용성을 제시하고자 하였다. 연구 결과 토털스테이션을 이용하여 지형이나 구조물의 변위를 측정할 수 있는 모니터링 시스템의 개념과 운영 흐름을 제시하였다. 모니터링 시스템은 현장상황에 따라 장비를 설치할 수 있도록 장비의 위치 및 운용 방식을 선택하고, 대상물에 대한 관측 횟수, 주기, 관측범위 등을 설정할 수 있도록 하였다. 모니터링 시스템에 대한 실험을 통해 5mm 이내의 정밀도로 측점을 모니터링 하였으며, 토털스테이션을 이용한 대상물의 변위 모니터링이 가능함을 제시하였다. 향후, 추가적인 연구를 통해 실제 사면이나 구조물에 대한 모니터링의 적용성을 제시할 수 있을 것으로 기대된다.

• 한국식품과학회지
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• 제48권1호
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• pp.59-65
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• 2016

본 연구는 배의 품종별 생육 단계에 따른 추출물의 총 페놀, 플라보노이드 함량 및 항산화 활성을 확인함과 동시에 세포 독성 및 멜라닌 생합성 억제능을 비교 평가하여 미백 소재로써의 사용 가능성을 평가하고자 국내 육성한 4가지 신품종과 신고 품종을 이용하여 비교 분석하였다. 총 폴리페놀 함량은 생육초기에 가장 높았으나 과일이 성숙할수록 낮아졌으며, 품종간에는 생육시기와 관계없이 추황배에서 높게 나타났다. 총 플라보노이드 함량은 총 폴리페놀보다 적은 함량으로 5품종 모두 생육시기별로 큰 차이를 보이지 않았다. 알부틴은 총 폴리페놀 함량과 같이 과일이 성숙할수록 낮아지는 경향을 보였으며 DPPH, ABTS 라디칼 소거활성을 분석한 항산화 활성도 감소의 폭은 달랐지만 비슷한 경향을 보였다. 높은 항산화 활성은 멜라닌 생합성 억제에도 효과적으로 작용하여 만개 후 90일까지는 감천배를 제외한 4품종에서 50% 내외의 높은 억제 활성을 보여, 이 시기까지의 배 과일 추출물은 우수한 미백활성을 가지는 것으로 확인되었다. 뿐만 아니라 세포독성이 극히 낮아 피부 안정성이 높은 기능성 천연 원료 및 미백 향장소재로써 이용 가능성이 충분한 것으로 판단된다.

• 한국동물위생학회지
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• 제33권4호
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• pp.387-392
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• 2010

This experiment was conducted to investigate the effect of fermented spent mushroom substrate (FSMS) on growth performance, blood profile, intestinal microflora and ammonia gas production of feces in broiler chickens. A total of three hundred sixty, 1-day-old male broiler chicks (Ross) were randomly divided into 3 groups with 6 replicates of 20 birds each. The treatments were control (free FSMS), 15% FSMS (basal diet with 15% FSMS) and 30% FSMS (basal diet with 30% FSMS). The final body weight and body weight gains were slightly improved in 30% FSMS than control (P<0.05). Feed intake and feed conversion were significantly improved as compared to those of the control groups. The Leukocytes of blood serum in FSMS groups were significantly decreased as compared to those of control groups. There are no significant differences among the groups in the contents of albumin (ALB), total cholesterol (TCHO), glucose (GLU), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in blood serum. The content of total glucose (TG) in 30% FSMS containing dietary groups was significantly decreased as compared to that of the control groups. The content of HDLC in 30% FSMS containing dietary groups was significantly increased as compared to that of the control group. The number of lactobacillus in the intestinal microflora were significantly increased in chicks fed FSMS groups. The ammonia gas production in FSMS groups was siginificantly decreased as compard to that the control groups. These results indicated that dietary FSMS exerted growth performance for feeding broiler.

• 한국전자통신학회논문지
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• 제19권3호
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• pp.515-522
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• 2024

전 세계적으로 전력 소비량이 꾸준히 증가하고 있으며, 특히 빌딩의 전력 소비 비율은 세계 전력 소비 비율의 20% 이상을 차지할 만큼 그 비중이 크다. 이에 따라 빌딩에서의 전력 소비를 효율적으로 관리하는 빌딩 에너지 관리 시스템(BEMS, Building Energy Management System)의 연구 및 개발이 활발히 진행되고 있으며, 특히 최근에는 인공지능 기술의 발달로 인해 Smart BEMS 연구가 주목받고 있다. 본 논문에서는 강화학습 기반 V2G(Vehicle-to-Grid) Station 연계형 스마트 에너지 빌딩 전력 제어 기법을 제안한다. 실제 빌딩의 전력량 데이터 기반 성능평가 결과, 학습이 진행됨에 따라 빌딩에서의 전력 요금이 감축하는 것을 확인하였다.

• 대한수의사회지
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• 제20권2호
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• pp.103-108
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• 1984

A total of 359 cultures of Staphylococcus were isolated from no raw milk samples in stock farms of Gyeongnam province from December, 1981 to May, 1982. Some biochemical properties and antimicrobial drug susceptibility of the isolaes were studied with the

• 한국측량학회:학술대회논문집
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• 한국측량학회 2004년도 추계학술발표회 논문집
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• pp.111-116
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• 2004

This study is given that on the bridge construction in marine by GPS, specially, choice of station for footing construction of marine bridge. You can get positive datum in the checking of marine excavation, if you use together Total Station and GPS. In the result of comparision of upper surveying methods, Surveying datum is gotten error of less than 30mm(The depth of water 10～l5mm, station 7～10mm). This study show that hereafter if you use GPS in bridge construction in marine you can get accurateiy datum.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2022년도 가을 학술논문 발표대회
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• pp.225-226
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• 2022

In order to secure the practicality of services using drones, improve on-site operability, and improve the convenience of aircraft maintenance, it is necessary to develop a drone station that can be safely stored while ensuring charging performance. Therefore, a total seven points of improvement were derived through laboratory experiments after the development of the first prototype, and an improved second prototype will be developed in the future based on the preceding processes.