• Title/Summary/Keyword: Total soil carbon

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Identification and Biochemical Characterization of a New Xylan-degrading Streptomyces atrovirens Subspecies WJ-2 Isolated from Soil of Jeju Island in Korea (제주도 토양으로부터 자일란 분해 Streptomyces atrovirens subspecies WJ-2 동정 및 효소의 생화학적 특성 규명)

  • Kim, Da Som;Bae, Chang Hwan;Yeo, Joo Hong;Chi, Won-Jae
    • Microbiology and Biotechnology Letters
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    • v.44 no.4
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    • pp.512-521
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    • 2016
  • A bacterial strain was isolated from a soil sample collected on Jeju Island, Korea. The strain, designated WJ-2, exhibited a high xylanase activity, whereas cellulase activity was not detected. The 16S rRNA gene sequence of WJ-2 was highly similar to type strains of the genus Streptomyces. A neighbor-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain WJ-2 is phylogenetically related to Streptomyces atrovirens. Furthermore, DNA-DNA hybridization analysis confirmed that strain WJ-2 is a novel subspecies of Streptomyces atrovirens. The genomic DNA G+C content was 73.98 mol% and the major fatty acid present was anteiso-C15:0 (36.19%). The growth and xylanase production of strain WJ-2 were significantly enhanced by using soytone and xylan as nitrogen and carbon sources, respectively. Crude enzyme preparations from the culture broth of strain WJ-2 exhibited maximal total xylanase activities at pH 7.0 and $55^{\circ}C$. Thin-layer chromatography analysis revealed that the crude enzyme degrades beechwood xylan to yield xylobiose and xylotriose as the principal hydrolyzed end products.

제강분진을 이용한 침출수의 화학적 산화처리

  • 장윤영;강정우;정재현;배범한;박규홍;장윤석
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2001.04a
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    • pp.107-110
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    • 2001
  • 제철소에서 매년 대량 발생되어 주로 매립처분되고 있는 제강분진의 재활용 방안으로서, 폐수처리분야에 널리 사용되고 있는 펜톤산화공정의 반응촉매원인 Fe 공급원으로서 제강분 진의 활용 가능성에 대한 연구를 수행하였다. 본 연구에서는 포항제철소에서 제철부산물로 발생되는 제강분진을 전처리 없이 산화촉매로 사용하여 김포 수도권 매립지의 침출수 처리 공정에서 펜톤산화조에 유입되는 원수를 대상으로 과산화수소에 의한 산화처리 실험을 수행하였다. 반응은 회분식으로 수행하였으며, 일반적으로 알려진 펜톤산화반응의 주요 반응조 건인 운전 pH, 과산화수소 주입량 및 분할주입, 제강분진의 주입량 등의 변화에 따른 각 조 건별 시간에 따른 반응결과를 알아보았다. 또한 기존의 Fe 공급원으로 사용되고 있는 FeSO$_4$와 처리성능 및 적용조건에 대한 비교 실험도 수행하였다. 침출수 수질변화는 TOC (Total Organic Carbon) analyzer를 사용하여 측정한 TOC값으로 나타냈으며, pH controller 와 정량펌프를 사용하여 HCl과 NaOH주입을 통해 반응기간동안 일정 pH를 유지하였다. 본 연구결과, 최적 pH 조건인 4에서 최대 75% TOC 제거율을 나타내었으며, 대부분의 반응은 30분 이내에 이루어졌다. 주어진 실험조건에서 FeSO$_4$와 비교하여 반응속도와 처리효율에서 향상된 결과를 나타내었으며 반응 후 응집침전실험에서도 보다 높은 처리효과를 얻을 수 있었다. 결론적으로, 과산화수소/제강분진 시스템을 이용한 화학적 산화처리방법은 경제성과 처리성능에서 기존의 펜톤산화공정의 대체방안으로서 향 후 적용가능성이 높을 것으로 기대된다.g, 200 mg/kg, 300 mg/kg의 순서로 함량이 점차 감소하는 결과를 얻을 수 있었다. 이상의 결과를 종합하여 볼 때 가자 메탄올추출물은 PQ 유도독성을 신장 및 폐조직에서 효과적으로 경감시키는 것으로 나타났다.ted retailers ("sellers") must accept end-of-life items returned to them by the consumers. At the local level, Taipei City implements a pay-as-you-throw program, whereby citizens pay waste collection and treatment fees through the purchase of special trash bags approved by the Taipei City Government. However. recyclables that are separated by citizens are collected free-of-charge by the City. Taichung City and Kaohsiung City, on the other hand, enforce mandatory sorting schemes, whereby citizens face penalties if they don't separate recyclables from the trash before pick-up. These programs have resulted in a significant reduction in municipal waste. Per capita waste collected per day has dropped from 1.143

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Location Environment and Vegetation Structure of the Aconitum austrokoreense Habitat (세뿔투구꽃 서식지의 입지환경 및 식생구조)

  • Cho, Seon-Hee;Lee, Kye-Han
    • Journal of Korean Society of Forest Science
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    • v.110 no.2
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    • pp.165-178
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    • 2021
  • Owing to the lack of consistent research on endangered plant species in Korea, there are insufficient data to preservespecies and expand habitats. This study analyzed the preferred habitat and threats to the survival of Aconitum austrokoreense, found on Baekwun Mountain in Gurye-gun, Gwangyang-si, Jeollanam-do Province, and classified as a level two endangered wild plant by the Ministry of Environment, by investigating major environmental factors such as climate, location, soil, and stand structure. By examining five selected sites inhabited by Aconitum austrokoreense on BaekwunMountain, this study found that the habitat had an altitude of 420 to 675 m above sea level and showed a northeast tendency, spreading over a range of inclination angles between 15° and 37°. The average number of plants across the five sites was 156. Site 4 (550 m) had the highest density of 372 plants, with an average height of 0.6 m. The average soil moisture and relative light intensity were 20.48% and 7.34%, respectively. Layer soil was presumed to be sandy loam, characterized by high sand content and good drainage. The habitat had average soil pH of 5.2, average organic matter of 16.46%, average nitrogen of 0.86%, average available phosphate of 11.86 mg/kg, average electrical conductivity of 0.44 dS/m, and average cation exchange capacity of 37.04 cmolc/kg. The total carbon in soil averaged 10.68%. From the analysis of the vegetation structure of sites inhabited by Aconitum austrokoreense, the dominant populations were Pinus koraiensis and Lindera erythrocarpa in Site 1, Magnolia obovata and Carpinus laxiflora in Site 2, Zelkova serrate and Quercus variabilis in Site 3, Staphylea bumalda and Lindera erythrocarpa in Site 4, and Morus bombycis,Styrax japonicus, and Carpinus laxiflora in Site 5. With most habitats located near trails and sap collection sites of Acer pictum, the species were exposed to artificial damage and interference threats.

Evaluation of Greenhouse Gas Emissions in Cropland Sector on Local Government Levels based on 2006 IPCC Guideline (2006 IPCC 가이드라인을 적용한 지자체별 경종부문 온실가스 배출량 평가)

  • Jeong, Hyun-Cheol;Kim, Gun-Yeob;Lee, Seul-Bi;Lee, Jong-Sik;Lee, Jung-Hwan;So, Kyu-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.5
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    • pp.842-847
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    • 2012
  • This study was conducted to estimate the greenhouse gas emissions on local government levels from 1990 to 2010 using 2006 IPCC guideline methodology. To calculate greenhouse gas emissions based on the 16 local governments, emission factor and scaling factor were used with default value and activity data came from the food, agricultural, forestry and fisheries statistical yearbook of MIFAFF (Ministry for Food, Agriculture, Forestry, and Fisheries). The total emissions in crop sector gradually decreased from 1990 to 2010 due to a decline in agricultural land and nitrogen fertilizer usage. The annual average emission of greenhouse gas was the highest in Jeonnam (JN) with 1,698 Gg $CO_2$-eq and following Chungnam (CN), Gyungbuk (GB), Jeonbuk (JB) and Gyunggi (GG). The sum of top-six locals emission had occupied 83.4% of the total emission in cropland sector. The annual average emissions in 1990 by applying 2006 IPCC guideline were approximately 43% less than the national greenhouse gas inventory by 1996 IPCC guideline. Jeonnam (JN) province occupied also the highest results of greenhouse gas emission estimated by gas types (methane, nitrous oxide and carbon dioxide) and emission sources such as rice cultivation, agricultural soil, field burning of crop residue and urea fertilizer.

Carbon and Nitrogen Inputs from Litterfall Components in Cryptomeria japonica and Chamaecyparis obtusa Plantations (삼나무와 편백 조림지의 낙엽·낙지에 의한 탄소 및 질소유입량)

  • Heejung Park;Gyeongwon Baek;Choonsig Kim
    • Journal of Korean Society of Forest Science
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    • v.113 no.1
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    • pp.97-106
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    • 2024
  • Evaluating carbon (C) and nitrogen (N) inputs from litterfall is important for soil nutrient management to enhance forest productivity and to understand the mechanisms of nutrient cycling in forest ecosystems. This study was conducted to compare C and N inputs from litterfall components of Cryptomeria japonica D. Don an d Chamaecyparis obtusa Endlicher planted in adjacent sites in the Jinju Research and Experimental Forests in Gyeongsangnam-do, South Korea. Litterfall into litter traps was collected at three-month intervals between December 2020 and December 2021, and the C and N concentrations of the litterfall components were measured. Litterfall amounts were not significantly different between the plantations, except for reproductive litterfall components. Litterfall accumulation peaked between December and March. The litterfall C concentration in the needle and seed litterfall was significantly higher for C. obtusa than for C. japonica. By contrast, the C concentrations in needle and flower litterfall differed seasonally. The mean N concentration of needle litterfall was significantly higher in C. japonica (0.96%) and C. obtusa collected between June and September (1.01%) than in the other seasons (C. japonica: 0.43%; C. obtusa: 0.53%). Carbon and N inputs in both plantations were highest in needle litterfall collected from December to March and lowest in needle litterfall collected from June to September. Annual C input by litterfall was similar between the plantations (C. japonica: 3,054 kg C ha-1 yr-1; C. obtusa: 3,129 kg C ha-1 yr-1), whereas total N input was higher for C. japonica (46.93 kg N ha-1 yr-1) than for C. obtusa (25.17 kg N ha-1 yr-1). The higher N input in the C. japonica plantation than in the C. obtusa plantation was associated with the input of reproductive components. These results could be applied to improve stand-scale models of C and N cycling by litterfall components in C. japonica an d C. obtusa plantations.

섭제골 지역의 산화지 및 비산화지의 군락구조 비교

  • Sim, Hak-Bo;Kim, Woen
    • The Korean Journal of Ecology
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    • v.16 no.4
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    • pp.429-438
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    • 1993
  • This is a report on the early vegetation and the secondary succession in the burned area of SeobJe-Go1 of $IIwasan-MY\v{o}n,\;Y\v{o}ngch\v{o}n-Gun,\;Ky\v{u}ngsangbuk-do$ Province. The forest fire occurred on April 8, 1982 and the pine forest and its floor vegetation were burned down. The investigation was done six times from August 20, 1982 to August 13, 1983. The results are summarized as follows: the floristic composition of burned areas $B_1,\;B_2$, and unburned areas $U_1,\;U_2$ were composed of 25, 23, 32, and 27 kinds of vascular plants. respectively. The biological spectra showed the $H-D_1-R_5-e$ type in both the burned and unburned areas. The species of Arundinella hirta, Miscanthus simnsis var. purpurascens and Cares hurnilis var. nana were dominant species in the burned area, while Pinus densiflorrr, Corex humilis var. nana and Rhododendron mucronulatum var. ciliafum were dominant species in the unburned area. Degree of succession of the unburned area was comparatively higher than that of the burned area. Species diversity index and evenness index of the burned area were similar to those of the unburned area. Indices of similarity in sampling sites showed that $B_1\;and\;B_2$ stands were the most similar. pH, total nitrogen, available phosphorus and exchangeable potassium of soil increased but organic matter and total organic carbon decreased after fire.

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Changes in Physico-chemical and Microbiological Parameters during Active Composting of Cattle Manure (우분 퇴비화의 주발효과정 중 이화학적 및 미생물학적 파라미터의 변화)

  • Kim, Yoon Seok;Kang, Myoung Kyu;Bae, Kyung Sook;Lee, Kyu Seung;Rhee, Young Ha
    • Korean Journal of Microbiology
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    • v.33 no.4
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    • pp.267-273
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    • 1997
  • Various physico-chemical and microbiological parameters of a composting system were compared with respect to their potential use for the monitoring and evaluation of composting processes for cattle manure. The temperature changed within a range of $30-65^{\circ}C$ during the whole composting process, and the period of active composting (>$40^{\circ}C$) persisted for 16 days. The concentrations of total carbon, total nitrogen, and organic matter decreased by 15% during active composting, but significant changes in C/N ratio were not observed. The decrease of temperature in the latter period of active composting caused a decrease of $NH_4^+-N$ and an increase of $NO_3^--N$ in the composting pile. When temperature exceeded $50^{\circ}C$, the population of thermophiles was higher than that of mesophiles by more than 1 or 2 orders of magnitude. Correlation analyses showed that amylase activity correlated positively with the population of mesophiles and reducing sugar content, but negatively with the population of thermophiles. Amylase activity was higher at the beginning of active composting, whereas cellulase, xylanase and ligninase activities which showed close relationship with each other, increased continually during active cornposting, suggesting the distinction of temporal niches between amylose-degrading and lignocellulose-degrading bacteria in the same habitat.

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Application of LCA on Lettuce Cropping System by Bottom-up Methodology in Protected Cultivation (시설상추 농가를 대상으로 하는 bottom-up 방식 LCA 방법론의 농업적 적용)

  • Ryu, Jong-Hee;Kim, Kye-Hoon;Kim, Gun-Yeob;So, Kyu-Ho;Kang, Kee-Kyung
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.6
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    • pp.1195-1206
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    • 2011
  • This study was conducted to apply LCA (Life cycle assessment) methodology to lettuce (Lactuca sativa L.) production systems in Namyang-ju as a case study. Five lettuce growing farms with three different farming systems (two farms with organic farming system, one farm with a system without agricultural chemicals and two farms with conventional farming system) were selected at Namyangju city of Gyeonggi-province in Korea. The input data for LCA were collected by interviewing with the farmers. The system boundary was set at a cropping season without heating and cooling system for reducing uncertainties in data collection and calculation. Sensitivity analysis was carried out to find out the effect of type and amount of fertilizer and energy use on GHG (Greenhouse Gas) emission. The results of establishing GTG (Gate-to-Gate) inventory revealed that the quantity of fertilizer and energy input had the largest value in producing 1 kg lettuce, the amount of pesticide input the smallest. The amount of electricity input was the largest in all farms except farm 1 which purchased seedlings from outside. The quantity of direct field emission of $CO_2$, $CH_4$ and $N_2O$ from farm 1 to farm 5 were 6.79E-03 (farm 1), 8.10E-03 (farm 2), 1.82E-02 (farm 3), 7.51E-02 (farm 4) and 1.61E-02 (farm 5) kg $kg^{-1}$ lettuce, respectively. According to the result of LCI analysis focused on GHG, it was observed that $CO_2$ emission was 2.92E-01 (farm 1), 3.76E-01 (farm 2), 4.11E-01 (farm 3), 9.40E-01 (farm 4) and $5.37E-01kg\;CO_2\;kg^{-1}\;lettuce$ (farm 5), respectively. Carbon dioxide contribute to the most GHG emission. Carbon dioxide was mainly emitted in the process of energy production, which occupied 67~91% of $CO_2$ emission from every production process from 5 farms. Due to higher proportion of $CO_2$ emission from production of compound fertilizer in conventional crop system, conventional crop system had lower proportion of $CO_2$ emission from energy production than organic crop system did. With increasing inorganic fertilizer input, the process of lettuce cultivation covered higher proportion in $N_2O$ emission. Therefore, farms 1 and 2 covered 87% of total $N_2O$ emission; and farm 3 covered 64%. The carbon footprints from farm 1 to farm 5 were 3.40E-01 (farm 1), 4.31E-01 (farm 2), 5.32E-01 (farm 3), 1.08E+00 (farm 4) and 6.14E-01 (farm 5) kg $CO_2$-eq. $kg^{-1}$ lettuce, respectively. Results of sensitivity analysis revealed the soybean meal was the most sensitive among 4 types of fertilizer. The value of compound fertilizer was the least sensitive among every fertilizer imput. Electricity showed the largest sensitivity on $CO_2$ emission. However, the value of $N_2O$ variation was almost zero.

Chemical and Spectroscopic Characterization of Peat Moss and Its Different Humic Fractions (Humin, Humic Acid and Fulvic Acid) (피트모스에서 추출한 휴믹물질(휴믹산, 풀빅산, 휴민)의 화학적 및 분광학적 물질특성 규명)

  • Lee Chang-Hoon;Shin Hyun-Sang;Kang Ki-Hoon
    • Journal of Soil and Groundwater Environment
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    • v.9 no.4
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    • pp.42-51
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    • 2004
  • Peat humin(p-Humin), humic acid(p-HA) and fulvic acid(p-FA) were isolated from Canadian Sphagnum peat moss by dissolution in 0.1M NaOH followed by acid precipitation. After purification cycles, they are characterized for their elemental compositions and, acid/base properties. Functionalities and carbon structures of the humic fractions were also characterized using FT-IR and solid state $^{13}C$-NMR spectroscopy. Those results are compared with one another and with soil humic substances from literatures. Main purpose of this study was to present a chemical and spectroscopic characterization data of humic substance from peat moss needed to evaluate its environmental applicability. The relative proportions of the p-Humin, p-HA and p-FA in the peat moss was $76\%,\;18\%,\;and\;3\%$, respectively, based on the total organic matter content ($957{\pm}32\;g/kg$). Elemental composition of p-Humin were found to be $C_{1.00}H_{1.52}O_{0.79}N_{0.01}$ and had higher H/C and (N+O)/C ratio compared to those of p-HA($C_{1.00}H_{1.09}O_{0.51}N_{0.02}$) and p-FA($C_{1.00}H_{1.08}O_{0.65}N_{0.01}$). Based on the analysis of pH titration data, there are two different types of acidic functional groups in the peat moss and its humic fractions and their proton exchange capacities(PEC, meq/g) were in the order p-FA(4.91) >p-HA(4.09) >p-Humin(2.38). IR spectroscopic results showed that the functionalities of the peat moss humic molecules are similar to those of soil humic substances, and carboxylic acid(-COOH) is main function group providing metal binding sites for Cd(II) sorption. Spectral features obtained from $^{13}C$-NMR indicated that peat moss humic molecules have rather lower degree of humification, and that important structural differences exist between p-Humin and soluble humic fractions(p-HA and p-FA).

Distribution of phosphorus in particle-size separates and specific gravity separates of soils (입경 및 비중별(比重別) 토양분화과 인산분포(燐酸分布))

  • Hong, Jung-Kook
    • Korean Journal of Soil Science and Fertilizer
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    • v.12 no.4
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    • pp.179-187
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    • 1980
  • 1) Soils (volcanic ash and muck) were fractionated into particle-size separates (200 - 20, 20 - 2, 2 - 0.5 and finer than $0.5{\mu}$ in diamter), and of which the silt fraction was further fractionated into specific gravity separates (more than 2.0, 2.0 - 1.7, 1.7 - 1.4 and less than 1.4 in $g/cc^3$). And total organic and inorganic phosphorus in the separates were determined. 2) The amounts of total, organic and inorganic phosphorus distributed in the particle-size separates were as follows fine clay > coarse clay > silt > fine sand fraction. The increase rate in the amounts of phosphorus was great in the separates finer than $20{\mu}$, and greatest in the fine clay fraction. 3) The amounts of total, oganic and inorganic phosphorus distributed in the specific gravity separates were as follows: 2.0 - 1.7 > 1.7 - 1.4 > heavier than 2.0 fraction. The increase rate in the amounts of phosphorus was in the following order 2.0 - 1.7 > 1.7 - 1.4 > heavier than 2.0 fraction. 4) Distribution of carbon, amorphous aluminum and free iron oxides in the particle-size separates and the specific gravity separates were examined, and the distribution and the formes of organo-minera1 complexes in the separates were discussed to shed light on the factors affecting the distribution of phosphorous into the separates. And it was estimated that there was close relation among the distribution of organic and inorganic phosphorus, and the distribution and the formes of organo-minera1 complexes.

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