• 제목/요약/키워드: Total phenolics

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Mechanisms of Hela Cell Apoptosis Induced by Abnormal Savda Munziq Total Phenolics Combined with Chemotherapeutic Agents

  • Zhang, Yun-Xia;Abliz, Guzalnur;Ye, Wei-Jun;Mutalipu, Zuohelaguli;Li, Xiao-Wen;Wang, Hai-Qin;Buranjiang, Gulimire;Upur, Halmurat
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권2호
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    • pp.743-747
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    • 2014
  • Objective: To investigate the effects of abnormal Savda Munziq (ASMq) total phenolics combined with cisplatin and docetaxel on the Hela cell growth. Methods: In vivo cultured Hela cells were treated with cisplatin, docetaxel, total phenolics, cisplatin+total phenolics or docetaxel+total phenolics. MTT was performed to assess inhibition of cell proliferation, flow cytometry to detect apoptosis, and semi-quantitative RT-PCR to test for survivin and Bcl-2 expression. Results: The total phenolics, cisplatin and docetaxel had significant inhibitory and apoptosis-promoting effects on Hela cells (P<0.05), with the early apoptotic rates of $12.8{\pm}0.70%$, $18.9{\pm}3.79%$ and $15.8{\pm}3.8%$; the total phenolics, cisplatin and docetaxel significantly decreased the expression of Bcl-2 and survivin (all P<0.01), especially when used in combination. Conclusion: ASMq total phenolics, combined with cisplatin and docetaxel, could promote the apoptosis of Hela cells possibly through reducing the expression of Bcl-2 and survivin.

Physicochemical qualities, antioxidant compounds, and activities of six mini paprika cultivars

  • Baek, Seolji;Shin, Youngjae
    • 한국식품과학회지
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    • 제52권4호
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    • pp.377-384
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    • 2020
  • Paprika is a popular vegetable with high visual appeal and desirable flavor, the health benefits of which are increasingly attracting interest. In this study, the physical qualities, antioxidant content, and activities of six mini paprika cultivars were investigated. Both the edible part (flesh) and the by-products were studied. The average total phenolics and total antioxidant activities were higher in the flesh than in the by-products. The total flavonoids of the flesh and the byproducts were 16.41 and 37.80 mg/100 g FW, respectively. "YW glory" and "Raon yellow" flesh had the highest (245.52 mg/100 g FW) and lowest (179.96 mg/100 g FW) total phenolics among the six cultivars, respectively. However, the "RD glory" cultivar showed the highest total phenolic content (232.70 mg/100 g FW) among the by-product samples. The total phenolics in the flesh and by-products were highly correlated to the ABTS radical scavenging activity, with R=0.961 and 0.984, respectively.

삼백초주(三白草酒) 발효과정의 성분 및 특성 변화 (Changes of Major Components in Sambaekcho Wine During Fermentation)

  • 김민자;김인재;남상영;이철희;신공식;임재윤
    • 한국약용작물학회지
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    • 제9권4호
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    • pp.290-294
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    • 2001
  • 삼백초주 제조중 기능성 성분의 이행성 정도를 측정하고자, 증자백미를 기준으로 개량누룩 5%, 양조용수 120%, 삼백초 건엽 분말 2% 또는 건근분말 5%를 첨가하여 삼백초주를 제조하였고, 발효기간에 따른 페놀성 화합물의 함량 변화를 조사하여 얻은 결과를 요약하면 다음과 같다. 1. 삼백초주의 total phenolics, flavonoid phenolics 및 quercetin 관련물질의 함량은 발효기간이 경과할수록 많았다. 2. 삼백초주의 total phenolics와 flavonoid phenolics 함량은 잎삼백초주에서 각각 ${762.5{\sim}1,054.7mg/l}$${387.8{\sim}537.2mg/l}$, 뿌리삼백초주는 각각 ${608.4{\sim}928.8\;mg/l}$${329.5{\sim}481.7\;mg/l}$로 잎삼백초주에서 높았다. 3. Quecetin 함량은 잎삼백초주에서는 ${3.5{\sim}3.8mg/l}$로 발효기간 간에 차이가 미미하였으며, 뿌리 삼백초주에서는 검출되지 않았다. Quercetin 관련 물질의 총함량은 잎삼백초주에서 ${72.3{\sim}84.2\;mg/l}$, 뿌리삼백초주 ${21.6{\sim}32.2\;mg/l}$로 잎삼백초주에서 높았다. 4. 뿌리삼백초주에 비하여 잎삼백초주에서 total phenolics, flavonoid phenolics 및 quercetin 관련물질의 함량이 현저히 높아 잎을 이용하여 삼백초주를 제조하는 것이 효과적이었다.

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Antioxidative Activities of Korean Apple Polyphenols

  • Kim, Yoon-Sook;Choi, Hee-Don;Choi, In-Wook
    • Preventive Nutrition and Food Science
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    • 제16권4호
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    • pp.370-375
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    • 2011
  • The antioxidative activity and the polyphenolic composition were examined in four different cultivars of apple (Malus domestica), 'Fuji', 'Tsugaru', 'Hongro' and 'Kogetsu', and their parts (peel, core, pulp and juice). The total phenolics, flavonoids and anthocyanins differed among the tested cultivars and parts. Peel parts had the highest total phenolics and anthocyanin content. Contributions of those phenolics to total antioxidative activity were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays, and the linoleic acid oxidation assay. Concentration of phenolics contributes significantly to the total antioxidative activity of apples. Clearly, apple peels, especially from Hongros and Kogetsus, possess high levels of phenolic compounds and antioxidants. Therefore, apple peels may potentially function as a value-added ingredient.

Antioxidant contents and activities of twelve varieties of vegetable sprouts

  • Park, Hyunjeong;Shin, Youngjae;Kim, Young-Jun
    • 한국식품과학회지
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    • 제51권3호
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    • pp.207-213
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    • 2019
  • This study was conducted to investigate the antioxidant contents and activities of twelve vegetable sprouts (broccoli, red radish, radish, mizuna, kale, taatsai, pak choi, Chinese cabbage, turnip, rapeseed, chicory, and alfalfa). The total flavonoid contents of the broccoli, red radish, and radish sprout were $25.36{\pm}0.13$, $25.26{\pm}1.80$, and $25.16{\pm}1.25mg$ CE/100 g FW, respectively, and were significantly higher than those of the other tested vegetables. Radish sprouts had the highest total phenolic content (112.42 mg GAE/100 g FW), followed by red radish and broccoli sprouts. The main polyphenols in the vegetable sprouts were epicatechin and chlorogenic acid, but they varied across sprout varieties. The correlation between total flavonoids and total phenolics for the 12 vegetable sprouts was very high (r=0.926). The total antioxidant activity (DPPH and ABTS radical scavenging activities) was also highly correlated with total flavonoids and total phenolics.

Total antioxidant capacity of the Korean diet

  • Han, Jeong-Hwa;Lee, Hye-Jin;Cho, Mi Ran;Chang, Namsoo;Kim, Yuri;Oh, Se-Young;Kang, Myung-Hee
    • Nutrition Research and Practice
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    • 제8권2호
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    • pp.183-191
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    • 2014
  • BACKGROUND/OBJECTIVES: The objective of this study was to measure and/or estimate the total antioxidant capacity of the Korean diet. MATERIALS/METHODS: Eighty-one plant foods that were expected to exhibit rather high antioxidant activities were selected from the Korean diet using the Fifth Korean National Health and Nutrition Survey (KNHANES V). These foods were categorized into 11 food groups: cereals, potatoes, legumes, nuts, vegetables, kimchies, mushrooms, fruits, fruit juices, sea weeds, and oils. The foods were mixed in the proportions specified in traditional Korean recipes and analyzed. The measured indicators for antioxidant capacities were total phenolics, 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), and Trolox equivalent antioxidant capacity (TEAC). RESULTS: Total phenolics were high in the fruit juices, nuts, vegetables, and fruits; and the average DPPH, ORAC, and TEAC values were high in the vegetables, fruits, fruit juices, and nuts. The correlation coefficient between the content of total phenolics of each food and the in vitro antioxidant capacity was relatively high at 0.851. The intake of total phenolics per capita per day in the Republic of Korea was estimated to be 127 mg. The total dietary antioxidant capacity (TDAC) values, which were obtained from the total antioxidant capacity of each food, taking into account the intake of each food, were 20,763, 54,335, and $876.4{\mu}mol$ of Trolox equivalents using the DPPH, ORAC, and TEAC methods, respectively. The food group that contributed the most to the Korean TDAC was cereals at 39.7%, followed by fruits and vegetables at 27.8% and 13.9%, respectively. The contribution of legumes, nuts, fruit juices, and mushrooms was quite minimal at less than 2% each. CONCLUSIONS: The content of total phenolics and the antioxidant capacity of the Korean diet are significantly correlated and the high contributing food groups are cereals, fruits, and vegetables.

Comparison of Antioxidant Potentials in Methanolic Extracts from Soybean and Rice Fermented with Monascus sp.

  • Pyo, Young-Hee
    • Food Science and Biotechnology
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    • 제16권3호
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    • pp.451-456
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    • 2007
  • The potential antioxidant activities of methanolic extracts from soybean and rice fermented with Monascus sp. were investigated. M. pilosus IFO 480 and M. anka IFO 478 were screened as a suitable strain to promote the antioxidant activities in soybean- and rice- fermentation. The methanol extracts from soybean and rice after fermenting for 20 days at $30^{\circ}C$ resulted in a significant increase in the antioxidant capacities expressed as radical (ABTS and DPPH) scavenging assay and peroxidation inhibition (%) by thiocyanate method and increased (p<0.01) by a 2.6 to 3.1-fold compared with those of the unfermented products. The average antioxidant potentials of Monascus-fermented soybean extracts (MFSE) were significantly (p<0.01) stronger than Monascus-fermented rice extracts (MFRE). A linear correlations between free radical scavenging activity of MFSE and the total phenolics content (r=0.84) and total flavonoids content (r=0.81) were observed. These results indicated that MFSE exhibited stronger (p<0.01) antioxidant activity and contained significantly higher levels (p<0.05) of phenolics than MFRE.

삼채의 잎, 인경, 뿌리의 총페놀 함량, 총플라보노이드 함량 및 항산화능 (Total Phenolics, Total Flavonoids, and Antioxidant Capacity in the Leaves, Bulbs, and Roots of Allium hookeri)

  • 황정승;이봉한;안향설;정하람;김영은;이인일;이형재;김대옥
    • 한국식품과학회지
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    • 제47권2호
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    • pp.261-266
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    • 2015
  • 삼채의 잎, 인경, 뿌리 세 부위를 물, 메탄올-물 혼합용매, 무수 메탄올을 이용하여 추출물을 제조한 후, 총페놀 함량, 총플라보노이드 함량 및 항산화능을 정량 분석하였다. 삼채의 부위별 총페놀 함량은 잎이 240.4-276.6 mg GAE/100 g FW, 인경이 65.5-82.8 mg GAE/100 g FW, 뿌리가 50.0-59.4 mg GAE/100 g FW를 보였다. 삼채의 부위별 총플라보노이드 함량은 잎에서 9.7-34.1 mg CE/100 g FW, 인경에서 5.0-16.7 mg CE/100 g FW, 뿌리에서는 4.4-15.3 mg CE/100 g FW로 나타났다. ABTS법, DPPH법, ORAC법을 통한 항산화능은 잎에서 각각 78.7-103.4, 24.4-59.0, 1,798.8-2,169.7 mg VCE/100 g FW, 인경에서는 35.8-51.1, 9.3-26.3, 415.3-611.7 mg VCE/100 g FW, 뿌리에서는 42.0-55.9, 12.8-24.4, 291.7-429.4 mg VCE/100 g FW을 보였다. 삼채 잎, 인경, 뿌리 중에서 잎이 가장 높은 총페놀 함량, 총플라보노이드 함량 및 항산화능을 가졌다. 6가지 추출용매에서 60% (v/v) 메탄올-물 혼합용매에서 주로 높은 활성을 나타냈다. 항산화능은 총플라보노이드 함량보다 총페놀 함량과 더 높은 상관관계를 보였다. 본 연구의 결과는 삼채가 높은 항산화능 공급할 수 있는 원료로서의 가능성을 보여 주었다는데 의의가 있다.

두과작물 새싹의 폴리페놀 함량 및 항산화성 비교 (Phenolics Content and Antioxidant Activity of Sprouts in Several Legume Crops)

  • 천상욱;김동관;김영민
    • 한국자원식물학회지
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    • 제26권2호
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    • pp.159-168
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    • 2013
  • 대두, 녹두, 및 동부 종자로 7일간 재배된 새싹나물의 생육, 폴리페놀 함량, 플라보노이드 함량, 항산화성 및 항산화효소 활성 차이를 검토하였다. 작물별 새싹나물의 총 신장은 녹두와 콩이 동부보다 유의적으로 컸으며 생체중은 오히려 콩과 동부가 녹두보다 유의적으로 높게 나타났다. Folin-Denis방법에 따른 총 페놀 함량은 콩나물의 메탄올 추출물(82.2 mg $kg^{-1}$)이 가장 높았으며, 그 다음이 동부나물(32.2 mg $kg^{-1}$), 녹두나물(24.5 mg $kg^{-1}$) 순으로 나타났다(p < 0.05). 한편, 총 플라보노이드 함량은 총 페놀 함량과 같은 경향을 보였으나 더 낮은 함량이 검출되었다. DPPH 라디컬 소거능은 추출물 농도가 증가할수록 높은 활성을 보였으며 전체적으로 낮은 활성이었으나 동부와 녹두 추출물(44와 42%)이 콩나물(25%)보다 비교적 높은 활성을 보였다. 항산화효소 활성은 APX와 POX활성은 동부가 가장 높았고 그 다음이 녹두, 콩 순으로 나타났고, CAT와 SOD 활성은 콩나물이 동부와 녹두나물보다 높게 나타났다. 따라서 본 연구는 총 페놀 함량($r^2$ = 0.53 ~ 0.90)과 총 플라보노이드 함량($r^2$ = 0.47 ~ 0.94)은 항산화성과 항산화효소 활성에 높은 연관성이 있으며, 그 함량과 활성은 작물별로 다르게 나타남을 확인하였다.

동부나물의 부위별 생육, 폴리페놀 및 항산화성 차이 (Difference in Growth, Phenolics Content and Antioxidant Activity of Cowpea Sprouts at Different Plant Parts)

  • 천상욱
    • 한국작물학회지
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    • 제58권3호
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    • pp.232-238
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    • 2013
  • 새싹재배기에서 7일간 재배된 동부 새싹나물의 부위별 생육, 폴리페놀 함량, 플라보노이드 함량, 항산화성 및 항산화효소 활성 차이를 검토하였다. Folin-Denis방법에 따른 총 페놀 함량은 동부나물 자엽의 메탄올 추출물(48.8 mg $kg^{-1}$)이 가장 높았으며, 그 다음이 뿌리(30.8 mg $kg^{-1}$), 하배축(22.2 mg $kg^{-1}$) 순으로 나타났다(p < 0.05). 한편, 총 플라보노이드 함량은 총 페놀 함량과 같은 경향을 보였으나 더 낮은 함량이 검출되었다. DPPH 라디컬 소거능은 추출물 농도가 증가할수록 높은 활성을 보였으며 동부나물 자엽의 추출물(82.5%)에서 가장 높았으며, 그 다음이 뿌리(52.6%), 하배축(35.0%) 순으로 나타났다(p < 0.05). 항산화효소 활성은 APX와 CAT 활성은 하배축에서 가장 높았고 POX와 SOD 활성은 뿌리가 가장 높은 활성을 보였다. 이런 경향은 재배 후 5일과 7일째 동부나물에서 같게 나타났다. 따라서 상관분석에 따르면 총 페놀 함량이 총 플라보노이드 함량보다 항산화성과 항산화효소 활성에 더 높은 관련성이 있는 것으로 나타났고 그 생리활성물질 함량과 그 활성 정도는 부위별로 다르게 나타났다.