• Journal of Microbiology and Biotechnology
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• v.18 no.3
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• pp.497-502
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• 2008

The essential oil from the cones of Pinus koraiensis was prepared after removing the seeds, and its chemical composition analyzed using gas chromatography-mass spectrometry (GC-MS). Hydrodistillation of the P. koraiensis cones yielded 1.07% (v/w) of essential oil, which was almost three times the amount of essential oil extracted from the needles of the same plant. Moreover, the antimicrobial activities of the oil against the growth of Gram-positive bacteria, Gram-negative bacteria, and fungi were evaluated using the agar disc diffusion method and broth microdilution method. Eighty-seven components, comprising about 96.8% of the total oil, were identified. The most abundant oil components were limonene (27.90%), ${\alpha}$-pinene (23.89%), ${\beta}$-pinene (12.02%), 3-carene(4.95%), ${\beta}$-myrcene (4.53%), isolongifolene (3.35%), (-)-bornyl acetate (2.02%), caryophyllene (1.71%), and camphene (1.54%). The essential oil was confirmed to have significant antimicrobial activities, especially against pathogenic fungal strains such as Candida glabrata YFCC 062 and Cryptococcus neoformans B 42419. Therefore, the present results indicate that the essential oil from the cones of Pinus koraiensis can be used in various ways as a nontoxic and environmentally friendly disinfectant.

• Korean Journal of Pharmacognosy
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• v.26 no.3
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• pp.276-306
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• 1995

The plant entries in the part of herbs in Ben Cao Gang Muwas classified according to the protocols of Engler and other botanists. Total numbers of plant written in chinese names were 635. Among them only 432 entries were able to be classified by elucidation of several references. They were classified as eight Divisions, 13 Classes, nine Subclasses, 60 Orders, 38 Suborders, 119 Families, 304 Genera, 377 Species with 25 Varieties and one Forma. According to the category of division, the Angiospermae was the largest group of 94 Families, 276 Genera, 348 Species, 24 Varieties and one Forma, whereas only one Genus and one Species belonged to Fungi and the Gymnospermae. Other 203 entries were unable to be classified because of their ambiguities in name.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.6
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• pp.474-481
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• 2013

Understanding the microbial community structure of agricultural soils is important for better soil management in order to improve soil quality. Phospholipid fatty acid analysis has been popularly used in determining the microbial community structure in different ecosystems. The microbial community structure of paddy soil under long-term fertilizer treatments was investigated after 45 years using PLFA analysis. Treatments were control (no fertilization, Con), compost (COM), NPK, NPK+compost (NPKC), PK, NK, and NP. Soil chemical properties were mainly affected by the addition of compost and inorganic P fertilizer. Total nitrogen and organic matter contents were significantly higher in treatments with compost while available $P_2O_5$ and exchangeable calcium were significantly higher in treatments with added inorganic P fertilizer. It was found that microbial communities were responsive to the different fertilizer treatments. PLFA results showed that the soils were dominated by gram-negative bacteria, followed by the actinomycetes, then gram-positive bacteria, and fungi. Principal component analysis of the soil chemical properties and PLFA composition proved to be a more reliable tool because it was more responsive to the changes in soil chemical properties.

• Journal of Environmental Science International
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• v.14 no.9
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• pp.811-815
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• 2005

The activated sludge from the aeration basin of the Su-yeong municipal wastewater treatment plant which has operated by a standard activated sludge process in Busan, Korea was investigated during April 2004 and January 2005 with several bio-indicators. The number of bacteria and fungi per gram of dry weight of MLSS were estimated to be $3.1\times10^6\sim1.5\times10^8\;and\;l.1\times10^3\sim1.1\times10^5$ colony forming units, respectively, by the plate agar method. By cultivation-independent methods, such as 4',6-diamidino-2-phenylindole stain and fluorescence in situ hybridization, the ratio of eubacteria to the entire biomass was evaluated by more than $80\%$ (v/v). The ratio of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria to the total eubacteria was detennined to be $7.0\sim9.8\%\;and\;3.3\sim6.2\%$ without heavy variation in spite of a period of relatively low temperature in the basin. It would be expected that the nitrification would occur or at least co-exist throughout the year in the sludge of many municipal WWTP with influents that contain the sufficient nitrogen sources although the WWTP does not have any specialized processes for the removal of nitrogen.

• Korean Journal of Environmental Biology
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• v.23 no.4
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• pp.383-389
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• 2005

The effect of different mulching materials on mungbean production was studied. The general objective was to assess the ecological effects of mulching materials in sustainable mungbean production. Specifically, the study aimed to determine the effects of different mulching materials on the chemical, physical and biological soil properties, on weed control and yield, and to identify mulching materials that are environmentally friendly in mungbean production. The experiment was conducted at the Fruit and Vegetables Seeds Center, Science City of $Mu/tilde{n}oz$, Nueva Ecija, Philippines from May to July 2004. The initial soil chemical properties were: pH of 6.4, 2.0 percent organic matter content, 0.10 percent total nitrogen, 22 ppm phosphorus, and 370 ppm available potassium. The soil microbial loads were $8\times10^4\;CFU\;g^{-1}$ for bacteria and $14\times10^4\;CFU\;g^{-1}$ for fungi. Mushroom spent mulch increased soil organic matter with an average of 3.13 percent, nitrogen with an average of 0.16 percent and the highest number of bacterial count with $3.4\times10^8\;CFU\;g^{-1}$. Use of mulch, except rice straw mulch, generally increased mungbean yield. The best mulching material for high yield production of mungbean was black polyethylene plastic film, although environmentally unfriendly.

• The Plant Pathology Journal
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• v.17 no.6
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• pp.357-360
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• 2001

Gray mold was observed on leaves of castor bean grown in Woniu and Okcheon in Korea in October 2000. Symptoms developed in the form of spot and blight with sporulation of the causal fungi at the marginal or central parts of the leaves. A total of 25 isolates were obtained from the infected leaves of castor bean. Out of the 25 isolates, 5 isolates which originated from Woniu were identified as Botrytis cinerea, while 20 isolates from Okcheon were identified as Amphobotrys ricini based on morphological and cultural characteristics. Two isolates each of B. cinerea and A. ricini were tested for their pathogenicity to castor bean plants. Gray mold symptoms similar to those observed in the fields were induced on leaves of castor bean by artificial inoculation. This is the first report of gray mold in castor bean caused by B. cinerea and A. ricini in Korea.

• The Plant Pathology Journal
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• v.18 no.1
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• pp.30-35
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• 2002

The hyphal growth and biocontrol efficacy of Trichodemo harzianum in soil may depend on its interactions with biotic components of the soil environment. The effect of soil microbial biomass on growth and biocontrol efficacy of T. hanianum isolate ThzIDl-M3 (green fluorescent protein transformant) was investigated using artificially prepared different levels of soil microbial biomass (153,328, or 517ug biomass carbon per g of dry soil; BC). The hyphal growth of T. harzanum was significantly inhibited in the soil with 328 or 517 $\mu$g BC compared with 153 ug BC. When ThzIDl-M3 was added to the soils as an alginate pellet formulation, the recoverable population of ThzIDl-M3 varied, but the highest population occurred in 517ug BC. Addition of alginate pellets of ThzIDl-M3 to the soils (10 per 50 g) resulted in increased indigenous microbial populations (total fungi, bacterial fluorescent Pseudomonas app., and actinomycetes). Furthermore, colonizing ability of ThzIDl-M3 on sclerotia of Sclerotinia sclerotiorum was significantly reduced in the soil with high revel of BC. These results suggest that increased soil microbial biomass contributes to increased interactions between introduced T. harzianum and soil microorganisms, consequently reducing the biocontrol efficacy of 1T. harzianum.

• Biomedical Science Letters
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• v.16 no.3
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• pp.187-192
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• 2010

The aim of this study was to compare the results of morphological phenotypic examination and molecular biological method, randomly amplified polymorphic DNA (RAPD) using clinical isolates from Korean patients infected with dermatophytes. A total of 44 clinical isolates have been collected by Korean Collection Medical Fungi (KCMF) and those were subjected for the RAPD analysis. The results showed that the fungal strains that have RG1 band patterns were identified as Trichophyton rubrum by the neighbour-joining analysis. Additionally, other fungal strains that have RG3 band patterns were identified as Epidermophyton floccosum and RG5 band patterns were identified as Micorsporum gypseum. Even though molecular biological method such as RAPD are not necessary, it might be useful when some strains have similar morphological characteristics or when same species has phenotypic variations. In this study, therefore, we targeted internal transcribed spacer (ITS) regions of fungal rDNA, performed RAPD and then compared with morphological phenotypic examination.

• Horticultural Science & Technology
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• v.30 no.6
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• pp.769-775
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• 2012

Among the phenolic compounds that is generally present in strawberry fruits, five simple phenolics, three flavonoids, and a stilbene were tested for their antimicrobial activity against seven fungi and one oomycete. trans-Cinnamic acid showed strong antimicrobial activity, and the antimicrobial effect of the simple phenolics decreased with an increase in the number of hydroxyl groups. Phytophthora capsici was the most susceptible to the phenolic compounds tested in this study. trans-Cinnamic acid, p-hydroxybenzoic acid, and kaempferol were mainly detected in 'Seolhyang' strawberry fruits, and the total phenolic contents of the fruits decreased during their development. Extracts of the green (1-10% red color) and red (above 90% red color) strawberry fruits reduced the mycelial growth and zoospore germination rate of P. capsici, and the extract of red strawberry fruit showed strong antimicrobial activity against the zoospore germination of P. capsici. These results indicate that strawberry fruits contain antimicrobial phenolic compounds and that strawberry fruit extract can be used as a natural fungistat.

• Clinical and Experimental Pediatrics
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• v.48 no.5
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• pp.461-468
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• 2005

The major function of immune system is to protect infections. The immune systems are composed of innate and adaptive immunity. In adaptive immunity, the cellular and humoral components interact each other. Neonates and infants are infected frequently, because immune systems are naive and easy to expose to infectious agents. The complete history and physical examination is essential to evaluate the child with recurrent infections. The environmental risk factors of recurrent infections are day care center, cigarette smoke, and air pollution. The underlying diseases such as immunodeficiency, autoimmune diseases, allergy, and disorders of anatomy or physiology increase the susceptibility to infections. In immunodeficiency, infections are characterized by severe, chronic, recurrent, and unusual microbial agents infection. The defects of antibody production are susceptible to sinopulmonary bacterial infections. T cells defects are vulerable to numerous organisms such as virus, fungi, bacteria and etc. The screening tests for immune functions are the quantitative and qualitative measurements of each immune components. A complete blood count with white blood cell, differential, and platelet provide quantitative informations of immune components. Total complement and immunoglobulin levels represent the humoral component. Antibody levels of previously injected vaccines also provide informations of the antigen specific antibody immune responses. T cell and subsets count is quantitative measurement of cell mediated immunity. Delayed hypersensitivity skin test is a crude measurement of T cell function. The long term outcome of children with recurrent infections is completely dependent on the underlying diseases, the initial time of diagnosis and therapy, continued management, and genetic counscelling.