• Journal of Embryo Transfer
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• v.29 no.3
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• pp.283-287
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• 2014

Sexed semen is commonly used for the production of calves of the desired gender. Gender selection is important in animal production industries. For example, female cattle are required for the dairy industry while males are preferred in the beef cattle industry. The present study was to assess the in vivo embryo production efficiency using the semen separated according to sex during superovulation in Hanwoo. Seventy Hanwoo donor cows were flushed on day 7 of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered on 7 days after the third insemination by flushing the uterus with embryo collection medium. KPN semen straws used artificial insemination contained 20 million sperm (total number 60 million per donor). Sex-sorted semen straws contained 4 million sperm (total number 12 million per donor). The results obtained were as follows: No differences were observed in the efficiency of superovulation rates on KPN semen 87%, and sexed semen 100%, respectively. The mean numbers of total embryos are each $12.58{\pm}8.31$ and $13.25{\pm}7.86$. The mean numbers of transferable embryos, sexed semen were significantly lower than KPN semen ($3.75{\pm}1.98$ vs. $8.23{\pm}6.07$, P<0.05). The rates of unfertilized embryos from superovulation using sexed semen were significantly higher than KPN semen (50% vs. 15%, P<0.05). The rate of degenerated 2-cell embryos from sexed and KPN semen was 60.87% and 11.11%, respectively (p<0.05). In conclusion, these results indicate that superovulation using sexed semen was useful, but efficient embryo production was important to reducing the damage caused by the Flowcytometer-based sperm sorting procedure.

• Journal of Embryo Transfer
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• v.25 no.3
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• pp.145-148
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• 2010

Oxygen consumption has been regarded as a useful indicator for assessment of mammalian embryo quality. This study was performed to investigate whether oxygen consumption reflects morphological grade of in vivo derived bovine blastocyst-stage embryos (blastocyst). The oxygen consumption of in vitro produced blastocyst was compared to its total cell number. In addition, pregnant rate was measured after transplantation of in vivo blastocysts with different oxygen consumption. The quality of blastocyst collected on day 7 after artificial insemination was categorized as grade I and II (G I and G II) based on microscopic observation of the morphology. Oxygen consumption of blastocyst was measured using a scanning electrochemical microscopy (SECM) and total cell number of in vitro blastocyst was enumerated by counting cells stained by propidium iodide. Pregnancy of recipient cow was confirmed with rectal palpation after 60 days of embryo transfer. The oxygen consumptions of G I blastocysts were significantly higher than those of G II blastocysts ($10.2{\times}10^{15}/mol\;s^{-1}$ versus $6.4{\times}10^{15}/mol\;s^{-1}$, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7, and 110.2 in the oxygen consumption of below 10.0, 10.0~12.0, and over $12.0{\sim}10^{15}/mol\;s^{-1}$ respectively. Total cell number was significantly increased in embryos with high oxygen consumption (p<0.05). Pregnant rate in recipient cow was 0, 50, and 85.7% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0, and over $12.0{\times}10^{15}/mol\;s^{-1}$, respectively. These results suggest that measurement of oxygen consumption may help increase the pregnant rate of bovine embryos.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.3
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• pp.320-324
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• 2012

The objective of this study was to evaluate the effect of spent mushroom substrate (SMS) derived from Pleurotus eryngii on the hematological and biochemical blood properties of elk. A total of 18, two and three-year-old elk were fed three different levels of SMS (0, 15 and 20%) in a corn-wheat bran diet for 80 days. The results indicated significantly high levels of blood monocytes, hemoglobin (Hb), and hematocrit (HCT) in elk fed 15% or 20% SMS (p<0.05) compared to control animals. Serum blood urea nitrogen (BUN) and glucose concentrations were also significantly elevated in elk fed both 15% and 20% SMS. The inclusion of SMS in the elk diet did not affect serum total cholesterol, triglyceride, or low density lipoprotein (LDL)-cholesterol concentrations; however, high density lipoprotein (HDL)-cholesterol concentration was significantly increased in SMS-fed groups. In addition, 20% SMS in the diet increased serum iron and testosterone concentrations in elk. These results indicate that adding SMS to the diet of elk can increase their Hgb, serum BUN, glucose, and HDL-cholesterol concentration; therefore, diets containing SMS may enhance the physiologic condition of elk during growth.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.4
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• pp.582-587
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• 2020

Recently, disasters caused by extreme weather and the damage caused by them are increasing worldwide. The interest in disasters, such as earthquakes, typhoons, and ground subsidence, is increasing in Korea. Korea has enacted a special law based on disaster management, and has built monitoring systems for individual facility units by building precision sensors and related systems to measure the displacement status of long bridges and high-rise composite buildings. On the other hand, the application of a real-time monitoring system is insufficient for slopes, open-pit mines, small and medium structures due to weather, measurement methods, cost, and constant monitoring difficulties. In this study, the displacement monitoring method using the total station was studied and the applicability was suggested through the experiment. Through the research, the concept and operation flow of a monitoring system that can measure the displacement of the terrain or the structure using the total station was presented. The monitoring system allows the user to select the location and operation method of the equipment so that the equipment can be installed according to the site situation, and set the number of observations, the period, and the observation range of the object. Using the experiment on the monitoring system, the station was monitored with precision within 5mm, and it was suggested that the displacement of the object can be monitored using the total station. Further research will be needed to assess the applicability of monitoring to real slopes and structures.

• Korean Journal of Food Science and Technology
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• v.48 no.1
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• pp.59-65
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• 2016

This study was carried out to investigate the total polyphenols, antioxidant activities, and melanin synthesis inhibition of several pear cultivars (Pyruspyrifolia). The total polypenolic content of five pear cultivars was high in the unripe developmental stage. Total polyphenolic content of the Chuwhangbae cultivar extract was higher than that of other pear cultivars regardless of the developmental stage. However, the total flavonoid content did not differ between cultivars or developmental stages. The phenolic compound, arbutin has an inhibitory effect on melanogenesis. Arbutin levels in pear cultivars declined as the fruit matured. The free radical scavenging activity of the extract also decreased as the fruit ripened. In B16F10 mouse melanoma cells, most of the cultivar extracts inhibited melanin synthesis by about 50% at a $100{\mu}g{\cdot}mL^{-1}$ concentration, except in the Gamcheonbae extract until 90 days after full bloom. We have confirmed that the extract of pear cultivars have antioxidant activity and skin-whitening effects.

• Korean Journal of Veterinary Service
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• v.33 no.4
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• pp.387-392
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• 2010

This experiment was conducted to investigate the effect of fermented spent mushroom substrate (FSMS) on growth performance, blood profile, intestinal microflora and ammonia gas production of feces in broiler chickens. A total of three hundred sixty, 1-day-old male broiler chicks (Ross) were randomly divided into 3 groups with 6 replicates of 20 birds each. The treatments were control (free FSMS), 15% FSMS (basal diet with 15% FSMS) and 30% FSMS (basal diet with 30% FSMS). The final body weight and body weight gains were slightly improved in 30% FSMS than control (P<0.05). Feed intake and feed conversion were significantly improved as compared to those of the control groups. The Leukocytes of blood serum in FSMS groups were significantly decreased as compared to those of control groups. There are no significant differences among the groups in the contents of albumin (ALB), total cholesterol (TCHO), glucose (GLU), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in blood serum. The content of total glucose (TG) in 30% FSMS containing dietary groups was significantly decreased as compared to that of the control groups. The content of HDLC in 30% FSMS containing dietary groups was significantly increased as compared to that of the control group. The number of lactobacillus in the intestinal microflora were significantly increased in chicks fed FSMS groups. The ammonia gas production in FSMS groups was siginificantly decreased as compard to that the control groups. These results indicated that dietary FSMS exerted growth performance for feeding broiler.

• Journal of the korean veterinary medical association
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• v.20 no.2
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• pp.103-108
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• 1984

A total of 359 cultures of Staphylococcus were isolated from no raw milk samples in stock farms of Gyeongnam province from December, 1981 to May, 1982. Some biochemical properties and antimicrobial drug susceptibility of the isolaes were studied with the

• Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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• 2004.11a
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• pp.111-116
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• 2004

This study is given that on the bridge construction in marine by GPS, specially, choice of station for footing construction of marine bridge. You can get positive datum in the checking of marine excavation, if you use together Total Station and GPS. In the result of comparision of upper surveying methods, Surveying datum is gotten error of less than 30mm(The depth of water 10～l5mm, station 7～10mm). This study show that hereafter if you use GPS in bridge construction in marine you can get accurateiy datum.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2022.11a
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• pp.225-226
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• 2022

In order to secure the practicality of services using drones, improve on-site operability, and improve the convenience of aircraft maintenance, it is necessary to develop a drone station that can be safely stored while ensuring charging performance. Therefore, a total seven points of improvement were derived through laboratory experiments after the development of the first prototype, and an improved second prototype will be developed in the future based on the preceding processes.

• Journal of the Korean Society of Tobacco Science
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• v.5 no.2
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• pp.3-7
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• 1983

Three levels of nitrogen, phosphate and potassium were applied to tobacco for the establishment of the optimum rate of the fertilizers for yield and quality. 1 . The rate increment of nitrogen and potassium increased tobacco growth, but phosphate were non - significant between rate. 2. The contents of total-nitrogen and total-alkaloid in the leaf were increased, with the increase of nitrogen rate while the effect of phosphate and potassium were negligible. 3. The yield and quality of the tobacco were increased with the increasing rate of nitrogen and potassium fertilizer but phosphate should little difference.