• Title/Summary/Keyword: Total Electron Content

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Studies on the Amino Acid, Sugar Analysis and Antioxidative Effect of Extracts from Artemisia sp. (쑥 추출물의 아미노산, 당 분석 및 항산화 효과에 관한 연구)

  • 최병범;이혜정;방선권
    • The Korean Journal of Food And Nutrition
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    • v.17 no.1
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    • pp.86-91
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    • 2004
  • This study has attempted to examine the antioxidative effect of ethanol extracts, and the analysis of amino acid and sugar of hot water extracts from Artemisia princeps, Artemisia argyi and Artemisia iwayomogi. Total phenol contents were 10.2 mg/$m\ell$, 4.7 mg/$m\ell$, and 7.0 mg/$m\ell$ in A. princeps, A. argyi and A. iwayomogi, respectively. Also, flavonoid contents were 6.1 mg%, 3.6 mg%, and 1.8 mg% in A. princeps, A. argyi and A. iwayomogi, respectively. Hot water extract from A. argyi showed highest electron donating ability toward DPPH. The contents of total amino acid by HPLC analysis were in the increasing order of A. princeps, A. argyi and A. iwayomogi. Of amino acid compositions, proline was largest in examined Artemisia sp.. The contents of total sugar by HPLC analysis were largest in A. iwayomogi. Of the sugar compositions, the glucose content was largest in examined all Artemisia sp., and the fructose content was in the increasing order of A. iwayomogi, A. argyi and A. princeps.

Application of Biosynthesized Silver Nanoparticles Against a Cancer Promoter Cyanobacterium, Microcystis aeruginosa

  • El-Sheekh, Mostafa Mohamed;El-Kassas, Hala Yassin
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.16
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    • pp.6773-6779
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    • 2014
  • Background: Nanotechnology opens new applications in many fields including medicine. Among all metallic nanoparticles, silver nanoparticles (silver NPS) have proved to be the most effective against a large variety of organisms including toxic cyanobacteria. Materials and Methods: Silver NPs were biosynthesized in vivo with different alga species namely, Spirulina piatensis, Chlorella vulgaris and Scenedesmus oh/iquus following two scenarios. First: by suspending a thoroughly washed algae biomass in 1 mM aqueous $AgN0_3$ solution. Second: by culturing them individually in culture media containing the same concentration of $AgN0_3$. Silver NPs were characterized using UV-Vis spectroscopy, transmission electron microscopy (TEM), energy dispersive analysis (EDX) and Fourier transform infra-red (FfIR) spectroscopy. The biosynthesized silver NPs were tested for cytotoxic activity against a cancer promoter cyanobacteruim Microcystis aeruginosa, considering effects on cell viability and chlorophyll content. Results: The surface plasmon band indicated the biosynthesis of silver NPs at ~400 nm. Transmission electron microscopy (TEM) revealed that the silver NPs had a mean average size below 100 nm. Energy-dispersive analysis X-ray (EDX) spectra confirmed the presence of silver element. FfIR spectral analyses suggested that proteins and or polysaccharides may be responsible for the biosynthesis of silver NPs and (-COO-) of carboxylate ions is responsible for stabilizing them. The toxic potentialities ofthe biosynthesized silver NPs against the cancer promoter cyanobacterium, Microcystis aeruginosa showed high reduction in viable cells count and the total chlorophyll content. Conclusions: The potential activity of the biosynthesized silver NPs from the studied algae species against Microcystis aernginosa cells is expected to be mainly mediated by the release of silver ions (Ag+) from the particle surface and bioactive compounds as indicated by FfIR analysis.

Optimization of Extraction Condition and Stability of Olive Leaf Extract (올리브 잎의 적정 추출조건 및 추출물의 안정성 조사)

  • Lee, Ok-Hwan;Lee, Hee-Bong;Lee, Jun-Soo;Lee, Boo-Yong
    • Korean Journal of Food Science and Technology
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    • v.37 no.2
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    • pp.178-182
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    • 2005
  • Basic optimal extraction condition and stability data were determined for prediction of usefulness of olive leaf as functional food material. Solid contents of olive leaf extracts increased with increasing extraction temperature and ethanol content, and was the highest (38%) under $85^{\circ}C$, 80% ethanol, and 5 hr treatment conditions, Total phenol contents and electron-donating abilities of olive leaf extracts also increased with Increasing ethanol content, and were the highest under $25^{\circ}C$, 80% ethanol, and 1 hr treatment conditions, then slightly decreased during storage at $25,\;55,\;and\;85^{\circ}C$. Olive leaf extract showed high stability under acidic storage condition, while low under alkalic condition.

Optimization of Cultivation and Storage Conditions on Red Cabbage Seed Sprouts (적양배추 새싹채소의 발아 및 저장 조건 최적화)

  • Baek, Kyeong-Hwan;Jo, Doekjo;Yoon, Sung-Ran;Kim, Gui-Ran;Park, Ju-Hwan;Lee, Gee-Dong;Kim, Jeong-Sook;Kim, Yuri;Han, Bumsoo;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.45 no.1
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    • pp.13-19
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    • 2013
  • This study was carried out to find the optimal conditions for red cabbage seed sprouts in terms of their physicochemical and sensory qualities by electron-beam irradiation, cultivation and storage using the response surface methodology (RSM). Moisture content ($R^2$=0.9638) was affected by irradiation dose and cultivation time. Total phenolics content ($R^2$=0.9117) was mainly affected by irradiation dose, but carotenoid content ($R^2$=0.8338) was affected in the order of irradiation dose, cultivation time and storage time. Sensory properties were also affected by irradiation dose, and thus scores decreased as irradiation dose increased. The optimum conditions estimated by superimposing total phenolics content and overall acceptance were 2.2-3.8 kGy of the irradiation dose, 3.0-4.0 days of cultivation and 2.0-3.0 days of storage.

Changes in Glucosinolate Content of Dolsan Leaf Mustard Kimchi during Fermentation and Correlation with Antioxidant, Antihypertensive, and Antidiabetic Activities (발효기간에 따른 돌산갓김치의 glucosinolates 함량변화와 항산화, 항고혈압 및 항당뇨활성과의 상관관계)

  • Oh, Sun-Kyung;Kim, Ki-Woong;Choi, Myeong-Rak
    • Journal of Life Science
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    • v.28 no.11
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    • pp.1290-1300
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    • 2018
  • The glucosinolate content, antioxidant activity, and antihypertensive and antidiabetic activities were measured in a crude extract of Dolsan leaf mustard kimchi (DLMK). The glucosinolate content was low at 6.41 and 7.92 mg/g in leaves and stems of DLMK after 21 days of fermentation. The total polyphenol and total flavonoid contents were more than 2 times higher in the leaves (211.7 mg GAE/g, 158.8 mg QE/g) than in the stem (53.7 mg GAE/g, 85.2 mg QE/g) during the fermentation period. The 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity and electron donating ability (EDA) were similar to those of the control group after 14 days of fermentation, while the ferric reducing antioxidant power (FRAP) was higher in the leaves after 14 days of fermentation when compared to the control group. The angiotensin converting enzyme (ACE) inhibitory activity showed similar or higher inhibitory activity in the leaves when compared to the control group (0.01% captopril), and the ${\alpha}$-glucosidase inhibitory activity was higher in the leaves and stems when compared to the control group (0.05% acarbose). The glucosinolate content and the ABTS, ACE, and ${\alpha}$-glucosidase inhibitory activity were correlated, as determined by the observed straight line plot with a positive grade. During the fermentation period, the detected glucosinolates were sinigrin, glucobrasicin, glucotropeolin, and progoitrin. The DLMK extract is therefore expected to be valuable as a functional food because of its effective antioxidant, antihypertensive, and antidiabetic activities.

The Effect of Far-Infrared Irradiation on Functional Components of Grape Seed (원적외선 처리가 포도씨의 기능성분에 미치는 영향)

  • Lee, Jihyun;Baek, Jiyoung;Yoon, Sung-Ran;Kwon, Joong-Ho
    • Current Research on Agriculture and Life Sciences
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    • v.28
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    • pp.53-62
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    • 2010
  • The effect of far-infrared (FIR) irradiation on the functional compounds of grape seeds extract were evaluated. Grape seed was dried on different heating power (0, 900, 1800 W) and heating time (0, 20, 40 min) with far-infrared drier. Contents of soluble solids, catechins, total phenolics and total flavonoids along with Hunter's color value, electron donating ability (EDA) and nitrite scavenging activity were analyzed. The soluble solids, Hunter's L value and a value were not significantly different from control samples. Cathechin, procyanidin $B_2$ and epicatechin contents changed with heating time. In the treated sample, total cathechins content was found maximum at 900 W of heating power and 20 min of heating time with a high level of total phenolics, total flavonids and EDA. These results indicated that FIR irradiation of grape seed could enhance antioxidant activities of its extracts by increasing the amounts of functional compounds. Moreover, response surface methodology(RSM) was applied to predict optimum conditions for heating by FIR rays of grape seeds. Based on superimposition of contour map with respect to total phenolics, total catechin and EDA, optimum ranges of heating conditions were heating power of 621.82~818.18 W and heating time of 16.3~19.83 min.

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Grain Quality of New Large-Grain Pigmented Rice Variety, "Daeripjamibyeo" (대립자미벼의 품질특성)

  • Ryu, Su-Noh
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.2
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    • pp.185-189
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    • 2013
  • Daeripjami, a novel black rice variety developed by conventional breeding has high contents of Cyanidin-3-glucoside(C3G) and a more strong antioxidant than normal black rice. This study aimed at informing ordinary people of the superiority of Daeripjami, a variety of rice proven to be superb physiologically and valuable as natural coloring, and making it popular by examing the physicochemical features of the rice. Recently, an increasing number of people in modern society are suffering from various adult diseases and atopic diseases. Therefore, in order to resolve the problem by making people consume more C3G, the main coloring contained in functional rice Daeripjami. The general components of Daeripjami, Superjami, Heugjinju and Ilpum were compared, As for water content, Ilpum took first place, followed by Heugjinju, Daeripjami and Superjami, As for crude protein and fat content, Heugjinju was highest, followed by Daeripjami, Superjami and Ilpum. This indicated that Daeripjami likely has better cooking qualities than Heugjinju. Amylose content turned out to be related with the volume, stickness and retrogradation of cook rice. As Heugjinju contained more amylose than Daeripjami, the latter was expected to have better eating quality than the former. The 1000 grain weight of daeripjami was 1.67 times heavier than that of Heugjinju. The C3G content of Daeripjami is 3.49 times higher compared with that of Heugjinju. As for total polyphenol and electron donating ability, Daeripjami was high in physiological functionality.

The Antioxidant Ability of Grape Seed Extracts (포도씨 추출물의 항산화성)

  • Jang, Jae-Kweon;Han, Ji-Young
    • Korean Journal of Food Science and Technology
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    • v.34 no.3
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    • pp.524-528
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    • 2002
  • The study was carried out to evaluate the antioxidant activity of grape seed extracts by measuring acid value, peroxide value (POV), thiobarbituric acid reactive substances (TBARS value) and electron donating ability by 1,1-dipheny1-2-pycryl hydrazil (DPPH) method. Total phenol content of freeze dried crude solvent extracts increased in the order of ethanol>acetone>hot water. Among extracts, the total phenol content of 70% ethanol extract of grape seed (GSE) was the highest, 51%. Besides the total phenol, the other major components of GSE were crude carbohydrate (29.63%), crude protein (3.38%) and crude lipid (2.84%). The acid values of crude rice bran oil containing GSE or several antioxidants at the concetration of 0.01% were 52.3 for the control, 0.5 for GSE, 2.3 for dibutyl hydroxytoluene (BHT), 45.0 for tertiarybutyl hydroxy quinone (TBHQ), 29.9 for tocopherol and 37.7 for ascorbic acid. The POVs for linoleic acid methyl ester in the presence of 0.01% antioxidants were 1220 meq/kg in control, 55 meq/kg in GSE, 104 meq/kg in BHT, 952 meq/kg in tocopherol and 71 meq/kg in GSE, 37% in BHT, 37% in tocopherol and 52% in ascorbic acid. The electron donating abilities by DPPH in the presence of 0.01% antioxidants were 95.3% in GSE, 75.0% in BHT, 96.3% in tocopherol and 98.2% in ascorbic acid. These results indicated that the antioxidants activities of GSE were significantly higher than those of several antioxidants compared.

Analysis of Antioxidant Activity, Total Phenol Content, and Flavonoid Content of Chungtaejeon Tea Extracts (청태전(Chungtaejeon Tea) 추출물의 항산화 활성과 총 페놀 및 플라보노이드 함량 분석)

  • GE, GE;Dong-Geun, Han;Hyun-Jeong, Kim;Eun-Young, Choi;Bong-Jeon, An
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.48 no.4
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    • pp.357-364
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    • 2022
  • In this study, antioxidant efficacy was analyzed to verify the value of use as an antioxidant-related functional cosmetic material using Cheongtaejeon tea extract. Cheongtaejeon tea was extracted, concentrated, and freeze-dried with hot water and 70% ethanol as a solvent to prepare samples, and then ABTS+ radical scavenging activity, electron donating ability, SOD-like ability, reducing power, ferric reducing antioxidant power (FRAP), total phenolic, and flavonoid contents were analyzed. In the ABTS+ radical scavenging activity experiment, both the Cheongtaejeon hydrothermal extract (CTW) and the Cheongtaejeon 70% ethanol extract (CTE) showed erasure activity of more than 98% at a concentration of 1,000 ㎍/mL, and in the electron donating analysis experiment, CTW and CTE, respectively, 42.20% and 78.82%. As a result of SOD-like activity measurement, activity of 39.73% and 67.39% of CTW and CTE, respectively, was confirmed. In the case of FRAP and reducing power experiments, both CTW and CTE showed high effects, and as a result of analyzing the total phenol and flavonoid contents, both CTW and CTE showed high contents. Based on the results of the experiment, the Cheongtaejeon tea extract is expected to have a highly valuable as a functional cosmetics material related to antioxidants.

Effect of Germinated Brown Rice Extract Powder on Free Amino Acid Content, Antioxidant and Nitrite Scavenging Ability of the Korean Cabbage Kimchi (동결건조 발아현미 추출분말이 배추김치의 유리아미노산 함량, 항산화성 및 아질산염 소거작용에 미치는 영향)

  • Woo, Seung-Mi;Jeong, Yong-Jin;Whang, Key
    • Food Science and Preservation
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    • v.13 no.5
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    • pp.548-554
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    • 2006
  • This study investigated the effect of the addition of germinated brown rice extract powder (0, 0.67, 3.35 and 6.70%) to the fermentation of Korean cabbage kimchi. During the fermentation of Korean cabbage kimchi with GBREP, content of total free amino acid and essential amino acid was showed higher value by increasing the addition of GBREP. In particular, ${\gamma}$-aminobutyric acid (GABA) content of Korean cabbage kimchi, prepared with 6.70% GBREP was 2 times higher than control Korean cabbage kimchi. By increasing the content of GBREP, total phenol content was showed high value and the content was more increased during fermentation except control Korean cabbage kimchi. By increasing the content of GBREP, electron donating activity and superoxide radical scavening activity were showed higher value and then showed maximum scavening activity on palatable period. Nitrite-scavenging ability of Korean cabbage kimchi with GBREP was higher than control Korean cabbage kimchi and showed the highest scavenging ability at pH 1.2.