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The Antioxidant Ability of Grape Seed Extracts  

Jang, Jae-Kweon (Department of Food Biotechnology, Chungkang College of Cultural Industries)
Han, Ji-Young (HaiTai Food Confectionery Research Center)
Publication Information
Korean Journal of Food Science and Technology / v.34, no.3, 2002 , pp. 524-528 More about this Journal
Abstract
The study was carried out to evaluate the antioxidant activity of grape seed extracts by measuring acid value, peroxide value (POV), thiobarbituric acid reactive substances (TBARS value) and electron donating ability by 1,1-dipheny1-2-pycryl hydrazil (DPPH) method. Total phenol content of freeze dried crude solvent extracts increased in the order of ethanol>acetone>hot water. Among extracts, the total phenol content of 70% ethanol extract of grape seed (GSE) was the highest, 51%. Besides the total phenol, the other major components of GSE were crude carbohydrate (29.63%), crude protein (3.38%) and crude lipid (2.84%). The acid values of crude rice bran oil containing GSE or several antioxidants at the concetration of 0.01% were 52.3 for the control, 0.5 for GSE, 2.3 for dibutyl hydroxytoluene (BHT), 45.0 for tertiarybutyl hydroxy quinone (TBHQ), 29.9 for tocopherol and 37.7 for ascorbic acid. The POVs for linoleic acid methyl ester in the presence of 0.01% antioxidants were 1220 meq/kg in control, 55 meq/kg in GSE, 104 meq/kg in BHT, 952 meq/kg in tocopherol and 71 meq/kg in GSE, 37% in BHT, 37% in tocopherol and 52% in ascorbic acid. The electron donating abilities by DPPH in the presence of 0.01% antioxidants were 95.3% in GSE, 75.0% in BHT, 96.3% in tocopherol and 98.2% in ascorbic acid. These results indicated that the antioxidants activities of GSE were significantly higher than those of several antioxidants compared.
Keywords
antioxidant activity; grape seed extract (GSE); acid value; POV; TBARS; DPPH;
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  • Reference
1 Ames, B.N. and Saul, R.L. Oxidative DNA damage, cancer and aging. Oxygen and human disease. Ann. Inter. Med. 107: 536-539 (1987)
2 Lee, Y.J. and Han, J.P. Antioxidative activides and nitrite scavenging abilities of extracts from ulmus devidiana. J. Korean Soc. Food Sci. Nutr. 29: 893-899 (2000)
3 Jorge, M. Ricardo, D.S., Jacques, R., Veronique, C., Annie, C. and Michel M. Procyanidin dimers and trimers from grape seeds. Phytochemistry 30: 1259-1264 (1991)   DOI   ScienceOn
4 Castillo, J., Benavente-Garcfa, 0., Lorente, J., Alcaraz, M., Redondo, A., Ortuno, A. and A Del Rio, J. Antioxidant activity and radioprotective effect against chromosomal damage induced m vivo by X-rays of fIavan-3-o1s(Procyanidins) from grape seeds (Vitis vinifera): Comparative study versus other phenolic and organic compounds. J. Agric. Food Chem. 48: 1738-1745 (2000)   DOI   ScienceOn
5 Yook, H.S., Kim, S.A., Jo, S.K. and Byun, M.W. Effect of gamma irradiation on the antioxidative activity and in vitro genotoxicol safety of red ginseng powder. J. Food Hyg. Safety 11: 41-50 (1996)
6 Park, C.O., Jin, S.H. and Ryu, B.H. Antioxidant activity of green tea extracts toward human low density lipoprotein. Korean J. Food Sci. Technol. 28: 850-858 (1996)
7 Shin, D.H. and Chung, J.K. Changes during storage of rice germ oil and its fatty acid composition. Korean J. Food Sci. Kechnol. 30: 77-81 (1998)
8 Halliwell, B. and Gutteridge, J.M.C. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem. J. 219: 1-14 (1984)   DOI
9 Blois, M.S. Antioxidant determinaon by the use of a stable free radical. Nature 26: 1199-1200 (1958)
10 Halliwell, B. and Gutteridge, J.M. Role of free radicals and catalytic metal ions in human disease: an overviews. Methods Enzymol. 186: 1-85 (1990)   DOI
11 Chancem, B., Sies, H. and Boveds, A. Hydroperoxide metabolism in mammalian oigans. Physiol. Rev. 59: 527-605 (1979)   DOI
12 Lim K.T. and Shim J.H. Antioxidative effects of ethanol extracts from Rhus Verniciftua Stokes (RVS) on mouse whole brain cells. Korean J. Food Sci. Technol. 29: 1248-1254 (1997)
13 AOAC. Official Methods of analysis. 16th ed., Association of Official Analytical Chemists, Washington, DC, USA, Vol. 2 chapter33, p.54 (1995)
14 Ariga, T. Antioxidative functions. Preventive action toward disease and utilization of proanthocyanidins. 日 本 油 化 學 會 誌 48: 1087-1096 (1999)
15 AOAC. Official Methods of Analysis. 16th ed., Association of Official Analytical Chemists, Washington, DC, USA, Vol. 2 chapter32, pp. 1-43 (1995)
16 Kang, M.H., Park, C.G., Cha, M.S., Seong, N.S., Chung, H.K. and Lee, J.B. Component characteristics of each extract prepared by different extract methods from by-products of Glycyrrhizia uralensis. J. Korean Soc. Food Sci. Nutr. 30: 138-142 (2001)
17 Ra, K.S., Suh, H.J., Chung, S.H. and Son, J.Y. Antioxidant activity of solvent extract from onion skin. Korean J. Food Sci. Technol. 29: 595-600 (1997)
18 Branen, A.L. Toxicological and biochemistry of butylatedhydroxyanisole and butylated hydroxytolune. J. Am. Oil Chem. Soc. 52: 59-63 (1975)   DOI   PUBMED
19 Ames, B.N., Shigenaga, M.K. and Hagen, T.M. Oxidants, antioxidants, and the degenerative diseases of aging. Proc. Natl. Acad. Sci. 90: 7915-7922 (1993)   DOI   ScienceOn
20 Lim, W.Y., Kim, J.S. and Moon, G.S. Antioxidative effect and characteristics of different model melanoidins with same color intensity. Korean J. Food Sci. Technol. 29: 1045-1051 (1997)