• Title/Summary/Keyword: Total AE Counts

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Changes of Physicochemical Properties and Microbial during Storage of Commercial Makgeolli (시판막걸리의 저장기간에 따른 품질 특성 및 미생물의 변화)

  • Kwon, Young-Hee;Lee, Ae-Ran;Kim, Jae-Ho;Kim, Hye-Ryun;Ahn, Byung-Hak
    • The Korean Journal of Mycology
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    • v.40 no.4
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    • pp.210-214
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    • 2012
  • The aim of this study was to determine changes of physicochemical properties and microorganisms of commercial Makgeolli during storage at $10^{\circ}C$ and $25^{\circ}C$ During storage at $10^{\circ}C$ for 11 days, ethanol contents did not increased up to 7.0%, and pH and total acidity were ranged with 3.12-3.99 and 0.22-0.28%, respectively. Makgeolli which was storaged at $25^{\circ}C$ for 11 days showed more than 7.0% of alcohol contents, pH of 3.96-4.17 and total acidity of 0.27-0.30%. Yeast cell counts showed maximal $2.90-16.00{\times}10^7$ CFU/mL at $10^{\circ}C$ for 3 days and $1.12-29.40{\times}10^7$ CFU/mL at $25^{\circ}C$ for 1 day. Lactic acid bacteria and total bacteria cell counts of Makgeolli which was storaged at $25^{\circ}C$ for 11 days were higher than those $10^{\circ}C$ storage. In preference test, Makgeolli from storage at $10^{\circ}C$ for 11 days was showed the level of 4.9-5.2 points however, Makgeolli from storage at $25^{\circ}C$ for one day showed below 5.0 point.

Study of the Somatic Cell Count Grade of Goat Milk in Korea (산양유의 체세포수 등급 설정에 관한 연구)

  • Shin, Ji-Hye;Jeong, Seok-Geun;Han, Gi-Sung;Jang, Ae-Ra;Chae, Hyun-Seok;Yoo, Young-Mo;Ahn, Chong-Nam;Woo, Kwang-Tae;Choi, Seok-Ho;Lee, Wan-Kyu;Ham, Jun-Sang
    • Food Science of Animal Resources
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    • v.28 no.2
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    • pp.218-221
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    • 2008
  • The aim of this work was to establish the standard for goat milk somatic cell counts. The data were obtained from MGEN, which were collected from Dec. 2006 to Nov. 2007. A total of three hundred and forty somatic cell counts from 12 goat milk farms were analyzed. A goat milk grading system by somatic cell count is proposed; less than 1,000,000/mL, 1,000,000-1,500,000/mL, 1,500,000-2,000,000/mL, 2,000,000/mL-2,500,000/mL, and over 2,500,000/mL. Under the grading system, the ratio of first grade goat milk would be 26.2%, and that of the fifth grade would be 11.8%. The first grade ratio is low and the fifth grade ratio is high compared to the cow milk grading system. It is expected that somatic cell counts of domestic goat milk will be improved by the proposed grading system.

Effect of Paecilomyces japonica on the Microbiological Quality and Shelf-life of Jeungpyun (눈꽃동충하초(Paecilomyces japonica)를 첨가한 증편의 미생물학적 품질특성 및 저장성)

  • Park Chan-Sung;Choi Mi-Ae;Park Geum-Soon
    • Korean journal of food and cookery science
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    • v.20 no.6 s.84
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    • pp.561-567
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    • 2004
  • The purpose of this study was to investigate the effect of Paecilomyces japonica mycelia(PJM) on pH, titrable acidity and microbiological qualify of Jeungpyun(fermented rice cake). Jeungpyun prepared with $0\~\%$ of PJM stored at $5^{\circ}C\;and\;20^{\circ}C$ for 4 weeks and 7 days respectively. Before fermentation of Jeungpyun dough, viable cells of total bacterial counts(TBC), yeasts and lactic acid bacteria(LAB) were $6.0\~9.8\times10^6,\;5.3\~9.0\times10^6,\;5.4\~8.5\times10^6\;CFU/g$, respectively. During the fermentation of dough, viable cells of TBC, yeasts and LAB increased $0.3\~0.4$ log cycle and pH was decreased whereas acidity increased as the progress of fermentation. Total viable cells in Jeungpyun before storage were $5.0\times10^1\;CFU/g$. During storage of Jeungpyun, TBC, yeasts and LAB of control group increased 2.6, 2.4, 2.1 log cycle at $5^{\circ}C$ and 4.8, 4.6, 4.5 log cycle at $50^{\circ}C$, respectively, when reached at maximum level. Major microflora of Jeungpyun was composed of yeasts and LAB during fermentation of dough and storage at $5^{\circ}C\;and\;20^{\circ}C$. Addition of PJM, inhibited the growth of microorganisms, the changes of PH and titrable acidity of Jeungpyun during storage at both of $5^{\circ}C\;and\;20^{\circ}C$. From these results, the addition of PJM extended the shelf-life of Jeungpyun during storage at $5^{\circ}C\;and\;20^{\circ}C$.

Non-specific Defensive Factors of the Pacific Oyster Crassostrea gigas against Infection with Marteilioides chungmuensis: A Flow-Cytometric Study

  • Choi, Hee-Jung;Hwang, Jee-Youn;Choi, Dong-Lim;Huh, Min-Do;Hur, Young-Baek;Lee, Nam-Sil;Seo, Jung-Soo;Kwon, Mun-Gyeong;Choi, Hye-Sung;Park, Myoung-Ae
    • Parasites, Hosts and Diseases
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    • v.49 no.3
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    • pp.229-234
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    • 2011
  • In order to assess changes in the activity of immunecompetency present in Crassostrea gigas infected with Marteilioides chungmuensis (Protozoa), the total hemocyte counts (THC), hemocyte populations, hemocyte viability, and phagocytosis rate were measured in oysters using flow cytometry. THC were increased significantly in oysters infected with M. chungmuensis relative to the healthy appearing oysters (HAO) (P<0.05). Among the total hemocyte composition, granulocyte levels were significantly increased in infected oysters as compared with HAO (P<0.05). In addition, the hyalinocyte was reduced significantly (P<0.05). The hemocyte viability did not differ between infected oysters and HAO. However, the phagocytosis rate was significantly higher in infected oysters relative to HAO (P<0.05). The measurement of alterations in the activity of immunecompetency in oysters, which was conducted via flow cytometry in this study, might be a useful biomarker of the defense system for evaluating the effects of ovarian parasites of C. gigas.

Study on Friction Welding of Copper to Aluminium for Developing Electrical Sleeve (전력용 슬리브 개발을 위한 동과 알루미늄의 마찰용접에 관한 연구)

  • 오세규;최진호;장지훈;오명석
    • Journal of Advanced Marine Engineering and Technology
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    • v.17 no.4
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    • pp.100-106
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    • 1993
  • A study on optimizing the friction welding of copper(C1100) to aluminium(A1050) for developing the electrical sleeve was experimentally carried out and also on real-time nondestructive evaluation of the friction weld quality (strength) was accomplished by acoustic emission technique. The results obtained are summarized as the following ; 1) The heating upset $U_1$(mm) or total upset U(mm) tends to increase according to the increase of heating time $t_1$(sec). The relations between $U_1$ and $t_1$ or U and $t_1$are computed as follows when n=2000rpm, $P_1$=4, $P_2$=8kgf/$mm^2$, and $t_2$=6sec. U=1.6$e^{0.39t_1}$ $U_1$=3.65$e^{0.25t_1}$. 2) It was notified that the proper welding conditions by considering on both strength with more than 100% joint effieciency and toughness are heating time of 1.5-2.25 sec under n=200rpm, $P_1$=4, $P_2$=8kgf/$mm^2$, $t_2$=6sec. 3) It was confirmed that both AE total counts(N, counts) and the weld tensile strength (${\sigma}$, kgf/$mm^2$) of the welded joints increase as the increase of heating time, respectively, the relations between N and $t_1$, ${\sigma}$ and $t_1$ are computed from data points by regression analysis using the least square method as follows in case of the above proper condition ; N=50108+23917(ln $t_1$)${\sigma}$$=11.85+2.06(ln $t_1$). 4) Both empirical and calcularated equations of relationship between .sigma. and N are very coincident with a high reliability, as the following in case of the above proper welding condition ; Calculated : ${\sigma}$=0.00008N+7.5 Empirical :${\sigma}$= $8.17e^{0.0000072N}$. 5) It was confirmed that the real-time nondestructive weld strength evaluation for friction welding of copper(C1100) to aluminium(A1050) could be possible by acoustic emission technique.

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Change of Quality Characteristics of Commercial and Prepared Kimchi Depending on Fermentation (상업용 김치와 담금 배추김치의 발효에 따른 품질특성 변화)

  • Nam, Dong-Geon;Kim, Sena;Choi, Youngmin;Jin, Yong-Xie;Yang, Mi-Ran;Han, Hye-Kyung;Choi, Ae-Jin
    • Korean journal of food and cookery science
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    • v.33 no.2
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    • pp.162-173
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    • 2017
  • Purpose: The purpose of this study was to evaluate the folate contents and quality characteristics of commercial and fermented Kimchi and compare the correlation between folic acid and quality characteristics. Methods: The contents of total folate and quality characteristics were evaluated in 10 kinds of industrial and local Kimchi and nine kinds of other commonly consumed Kimchi. Changes in folate content and quality characteristics of Kimchi during 14 days of fermentation at $15^{\circ}C$ were compared. Results: Chungnam (L4) Kimchi had the lowest pH of 4.62, and acidity (0.57%) and salinity (3.26%) were highest compared to other areas. The content of total folate of D company (A) was the highest at $73.66{\mu}g/100g$. The content of total folate was significantly higher than those of Sesame leaf Kimchi (O9) and Young leafy radish Kimchi (O5) containing 65.77 and $62.82{\mu}g/100g$, respectively. The pH of fermented Kimchi decreased from 6.09 to 4.11 (p<0.05), and the acidity significantly increased (p<0.001) to 0.32-0.66%. Reducing sugar content decreased rapidly by 4 days and then slowly decreased (p<0.001). Total bacteria and lactic acid bacteria counts significantly increased (p<0.05) with fermentation period. Content of total folate was highest at $69.82{\mu}g/100g$ and $68.16{\mu}g/100g$ on days 0 and 2 of fermentation, after which it decreased to 77.6% at $15.61{\mu}g/100g$ on day 10 of fermentation. Conclusion: As a result, there was no definite trend regarding folate content in commercial Kimchi and other Kimchi. Young leafy radish, Chives, and Welsh onion Kimchi, which are rich in green leaves, are excellent folate source foods. The highest folate content of fermented Kimchi was identified on day 2 of fermentation. Therefore, it is recommended to use raw or immature Kimchi when using Kimchi as a folate source food.

Investigation of Norovirus Occurrence and Influence of Environmental Factors in Food Service Institutions of ChungCheong Area (충청지역 집단급식소의 노로바이러스 실태조사와 환경요인의 영향)

  • Jung, Woo-Young;Eom, Joon-Ho;Kim, Byeong-Jo;Yun, Min-Ho;Ju, In-Sun;Kim, Chang-Soo;Kim, Mi-Ra;Byun, Jung-A;Park, You-Gyoung;Son, Sang-Hyuck;Lee, Eun-Mi;Jung, Rae-Seok;Na, Mi-Ae;Yuk, Dong-Yeon;Gang, Ji-Yeon;Heo, Ok-Sun
    • Journal of Food Hygiene and Safety
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    • v.25 no.2
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    • pp.153-161
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    • 2010
  • The purpose of this study was to examine the appearance of norovirus in the water for food in food service institutions and the influence of physicochemical and microbial factors of norovirus in order to work out basic data to predict the detection of norovirus. Among 82 samples of water for food in food service institutions, norovirus appeared in 7 samples and the rate of appearance was 8.5%. As for the type of norovirus, one samples contained GI type (genotype GI-6) and six samples contained GII type (genotype GII-2, GII-4, GII-12). In the regression model of prediction of norovirus, the rate of appearance was correlated with $NH_3$-N, total solids and the consumption of $KMnO_4$, out of such variables as $NH_3$-N, total solids, the consumption of $KMnO_4$, depth, chloride and total colony counts, and its contribution rate for effectiveness was 78.60%. In order to examine the influential factor of environment upon the detection of norovirus, Pearson's correlation analysis was carried out. The predictable regression formula for appearance rate of norovirus was expressed as -1.818 + 42.677 [$NH_3$-N] + 0.023 [total solids] + 0.762 [consumption of $KMnO_4$] -0.009 [depth] -0.146 [chloride] + 0.007 [total colony counts] (R = 0.904, $R^2$ = 0.818, adjusted $R^2$ = 0.786, p < 0.05). The most influential factors upon the detection of norovirus were $NH_3$-N, total solids and the consumption of $KMnO_4$. In other words, when the measured values of $NH_3$-N, total solids and the consumption of $KMnO_4$ were higher, the possibility of appearance of norovirus increased.

Effects of Extruded Pellet on Growth and Health Parameters in Farm Cultured Olive Flounder Paralichthys Olivaceus (배합사료가 양식장 넙치의 성장 및 건강도 향상에 미치는 영향)

  • Kim, Kang-Woong;Kwon, Mun-Gyeong;Kim, Kyoung-Duck;Kim, Shin-Kwon;Park, Myoung-Ae;Son, Maeng-Hyun
    • Journal of Fisheries and Marine Sciences Education
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    • v.22 no.4
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    • pp.529-536
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    • 2010
  • This study was conducted to effect of extruded pellets (EPs) and moist pellet (MP) diet on growth and fish health parameters in farm cultured olive flounder Paralichthys olivaceus. Two replicate groups of 2,600 fish per each tank (initial weight of $30.1{\pm}0.2$ g) were fed one of the three EPs (EP1, EP2 and CEP) and one MP for 13 months. In field feeding experiment conducted in commercial flounder farm, survival, growth (30-600 g) and condition factor of fish fed experimental EP1 and EP2 were comparable to those of fish fed MP, but considerably higher feed efficiency and protein efficiency ratio were observed in fish fed experimental EPs. Viable cell counts from diet and water from MP diet were considerably higher than those from EP diet, and lysozyme activity from EP diet was higher than that from MP diet. GOT and GPT of fish fed the EP diet were considerably lower than those from MP group, but total protein and glucose concentration of fish fed the EP groups were not significantly different from that of fish fed the MP. Therefore, these results strongly suggest that EP diets could be developed to replace MP diet without adverse effects on growth performance and to enhance the immunity and protective ability of olive flounder against fish disease such as Vibro, Edwardsiella and Strptococcus.

Effect of Dietary Mulberry leaf on the Composition of Intestinal Microflora in SD Rats (식이 뽕잎이 흰쥐의 장내균총 조성에 미치는 영향)

  • Lee, Heui-Sam;Jeon, Ho-Jung;Lee, Sang-Duk;Moon, Jae-Yu;Kim, Ae-Jung;Ryu, Kang-Sun
    • Korean Journal of Food Science and Technology
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    • v.33 no.2
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    • pp.252-255
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    • 2001
  • This study was performed to investigate the influence of dietary mulberry leaf on the intestinal microflora in rats. Rats were fed each experimental diets containing 1%, 10% of mulberry leaf powder for 4 weeks. Total viable counts and the numbers of Bifidobacterium, Lactobacillus, Clostridium, E. coli and Staphylococcus were determined by nonselective media and various selective media. A decrease in the intestinal population of Clostridium was shown in dietary mulberry leaf group. The E. coli and Staphylococcus populations decreased in dietary mulberry leaf group compared with control group. Methanol extract and fractions of mulberry leaf were subjected to an in vitro screening test for their growth-inhibitory activity. Methanol extract and Water fraction of Mulberry leaves showed weak growth-inhibition of Clostridium perfringens. These results indicate that the composition of gastrointestinal microflora was improved by treatment of mulberry leaves in SD rats and was very effective for growth inhibition of the intestinal harmful bacteria in intestine. Therefore, the mulberry leaves as a newly bio-material can be a useful material for physiologically functional food.

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Quality and Sensory Characteristics of Beef Jerky Prepared with Fermented Fruit Wines (발효 과실주 첨가에 따른 육포의 품질 및 관능 특성)

  • Choi Kyung-Ae;Cho Eun-Ja
    • Journal of the East Asian Society of Dietary Life
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    • v.15 no.2
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    • pp.171-181
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    • 2005
  • In this study, to investigate the effect of fermented fruit wines on quality and sensory characteristics of beef jerky physicochemical properties and sensory evaluation were determined. Acidity of fruit wines were high in order of Kiwifruit wine>Pineapple wine>Red grapes wine>Pear wine. Aw and pH of beef jerky added fruits wine decreased slowly with the extended storage period and these trend were obvious in jerky added kiwifruit wine. Textural characteristics values of all beef jerky samples were increased according to the storage period was prolonged. At 4 weeks of storage, beef jerky-added Kiwifruit wine showed the highest value in chewiness. The L, a, b values of all beef jerky samples showed a tendency to decrease with the extended storage period, and beef jerky-added kiwifruit wine were the highest value in L, a, b, at 4 weeks of storage. TBA values of beef jerky-added kiwifruit wine, Chungju (S) and pear wine (P) were $0.51\;\cal{mg/kg}$, $0.71\;and\;0.78\;\cal{mg/kg}$ respectively. Total plate counts of bacteria of all beef jerky-added fruit wines were lower than those of Control (Con) and increased as storage period was prolonged and beef jerky-added kiwifruit wine were the lowest value $9.0\times10^{5} CFU/g$ at 4 weeks of storage. In the sensory evaluation, beef jerky-added fruit wines showed higher score than those of Control (Con), and All beef jerky samples got high score at 1 week' of storage. Beef jerky-added Kiwifruit wine showed high score in almost sensory items and especially in overall.

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