Browse > Article
http://dx.doi.org/10.3347/kjp.2011.49.3.229

Non-specific Defensive Factors of the Pacific Oyster Crassostrea gigas against Infection with Marteilioides chungmuensis: A Flow-Cytometric Study  

Choi, Hee-Jung (Pathology Division, National Fisheries Research and Development Institute (NFRDI))
Hwang, Jee-Youn (Pathology Division, National Fisheries Research and Development Institute (NFRDI))
Choi, Dong-Lim (Pathology Division, National Fisheries Research and Development Institute (NFRDI))
Huh, Min-Do (Department of Aquatic Life Medicine, Pukyong National University)
Hur, Young-Baek (Southeast Sea Fisheries Research Institute, NFRDI)
Lee, Nam-Sil (Pathology Division, National Fisheries Research and Development Institute (NFRDI))
Seo, Jung-Soo (Pathology Division, National Fisheries Research and Development Institute (NFRDI))
Kwon, Mun-Gyeong (Pathology Division, National Fisheries Research and Development Institute (NFRDI))
Choi, Hye-Sung (Pathology Division, National Fisheries Research and Development Institute (NFRDI))
Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute (NFRDI))
Publication Information
Parasites, Hosts and Diseases / v.49, no.3, 2011 , pp. 229-234 More about this Journal
Abstract
In order to assess changes in the activity of immunecompetency present in Crassostrea gigas infected with Marteilioides chungmuensis (Protozoa), the total hemocyte counts (THC), hemocyte populations, hemocyte viability, and phagocytosis rate were measured in oysters using flow cytometry. THC were increased significantly in oysters infected with M. chungmuensis relative to the healthy appearing oysters (HAO) (P<0.05). Among the total hemocyte composition, granulocyte levels were significantly increased in infected oysters as compared with HAO (P<0.05). In addition, the hyalinocyte was reduced significantly (P<0.05). The hemocyte viability did not differ between infected oysters and HAO. However, the phagocytosis rate was significantly higher in infected oysters relative to HAO (P<0.05). The measurement of alterations in the activity of immunecompetency in oysters, which was conducted via flow cytometry in this study, might be a useful biomarker of the defense system for evaluating the effects of ovarian parasites of C. gigas.
Keywords
Marteilioides chungmuensis; pacific oyster; Crassostrea gigas; flow cytometry; hemocyte;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Jeong WG, Seo JH, Cho SM, Park CI. Influence of environmental factors on the prevalence of the ovarian parasite, Marteilioides chungmuensis, in Crassostrea gigas cultured in Pukman Bay, Tong-yeong, Korea. Korean J Malacol 2005; 21: 33-40.
2 Tun KL, Itoh N, Komiyama H, Ueki N, Yoshinaga T, Ogawa K. Comparison of Marteilioides chungmuensis infection in the Pacific oyster, Crassostrea gigas cultured in different conditions. Aquaculture 2006; 253: 91-97.   DOI
3 Glinski Z, Jarosz J. Molluscan immune defenses. Arch Immunol Ther Exp 1997; 45: 149-155.
4 Hooper C, Day R, Slocombe R, Handlinger J, Benkendorff K. Stress and immune responses in abalone: Limitations in current knowledge and investigative methods based on other models. Fish Shellfish Immun 2007; 22: 363-379.   DOI   ScienceOn
5 Ashton-Alcox KA, Ford SE. Variability in molluscan hemocyte: A flow cytometric study. Tissue Cell 1998; 30: 195-204.   DOI   ScienceOn
6 Goedken M, DeGuise S. Flow cytometry as a tool to quantify the oyster phagocytosis, respiratory burst, and apoptosis. J Shellfish Res 2002; 21: 342.
7 Donaghy L, Hong HK, Lambert C, Park HS, Shim WJ, Choi KS. First characterisation of the populations and immune-related activities of hemocytes from two edible gastropod species, the disk abalone, Haliotis discus discus and the spiny top shell, Turbo cornutus. Fish Shellfish Immun 2010; 28: 87-97.   DOI   ScienceOn
8 Donaghy L, Kim BK, Hong HK, Park HS, Choi KS. Flow cytometry studies on the populations and immune parameters of the hemocytes of the Suminoe oyster, Crassostrea ariakensis. Fish Shellfish Immun 2009; 27: 296-301.   DOI   ScienceOn
9 Itoh N, Oda T, Yoshinaga T, Ogawa K. DNA probes for detection of Marteilioides chungmuensis from the ovary of Pacific oyster Crassostrea gigas. Fish Pathol (Tokyo) 2003; 38: 163-169.   DOI
10 Tun KL, Itoh N, Shimizu Y, Yamanoi H, Yoshinaga T, Ogawa K. Pathology of the protozoan parasite Marteilioides chungmuensis in the Pacific oyster, Crassostrea gigas. Int J Parasitol 2008; 38: 211-217.   DOI   ScienceOn
11 Park MS, Kang CK, Choi DL, Jee BY. Appearance and pathogenicity of ovarian parasite Marteilioides chungmuensis in the farmed Pacific oysters, Crassostrea gigas, in Korea. J Shellfish Res 2003; 22: 475-479.
12 Tun KL, Itoh N, Ueki N, Yoshinaga T, Ogawa K. Relationship between Marteilioides chungmuensis infection in the Pacific oyster, Crassostrea gigas. J Invertebr Pathol 2007; 96: 205-212.   DOI   ScienceOn
13 Rodrick GE. Effects of temperature, salinity and pesiticides on oyster hemocyte activity. Florida Water Resources J 2008; 1: 86-101.
14 Lee YC. The Study on the Development of Oyster Rack Culture System. Inha University. Maritime Affairs and Fisheries. Final Research Report, Seoul, Korea. 1999, p 32-38.
15 Park MS, Lyu HY, Lee TS. Investigation on the cause of bad natural seed collection of the pacific oyster, Crassostrea gigas: Relationships between the conditions of mother shell and the viability of the released eggs and larvae based on the pathological and embryological survey. J Korean Fish Soc 1999; 32: 62-67.
16 Comps M, Park MS, Desports I. Fine structure of Marteilioides chungmuensis n.g., n. sp., parasite of oocyte of the oyster Crassostrea gigas. Aquaculture 1987; 67: 264-265.   DOI   ScienceOn
17 Itoh N, Oda T, Ogawa K, Wakabayashi, H. Identification and development of a paramyxean ovarian parasite in the Pacific oyster Crassostrea gigas. Fish Pathol (Tokyo) 2002; 37: 23-28.   DOI
18 Itoh N, Tun KL, Komiyama H, Ueki N, Ogawa K. An ovarian infection in the Iwagaki oyster, Crassostrea nippona, with the protozoan parasite Marteilioides chungmuensis. J Fish Dis 2004; 27: 311-314.   DOI   ScienceOn
19 Park KI, Park HS, Kim JM, Park YJ, Hong JS, Choi KS. Flow Cytometric Assessment of Immune Parameters of the Manila Clam (Ruditapes philippinarum). J Korean Fish Soc 2006; 39: 123-131.
20 Choi DL, Lee NS, Kim MS, Seo JS, Park MA, Kim JW, Hwang JY. Flow cytometry analysis of softness syndrome effects on hemocytes of the tunicate Halocynthia roretzi. Aquaculture 2010; 309: 25-30.   DOI
21 Carballal MJ, Villalba A, Lopez C. Seasonal variation and effects of age, food availability, size, gonadal development, and parasitism on the hemogram of Mytilus galloprovincialis. J Invertebr Pathol 1998; 72: 304-312.   DOI   ScienceOn
22 Hegaret H, Da Silva PM, Wikfors GH, Lambert C, De Bettignies T, Shumway SE, Soudant P. Hemocyte responses of Manila clams, Ruditapes philippinarum, with varying parasite, Perkinsus olseni, severity to toxic-algal exposures. Aquat Toxicol 2007; 84: 469-479.   DOI   ScienceOn
23 Hine PM. The inter-relationships of bivalve haemocytes. Fish Shellfish Immun 1999; 9: 367-385.   DOI   ScienceOn
24 Bushek D, Ford SE, Alcox KA, Gustafson R, Allen Jr, SK. Response of Crassostrea virginica to in vitro cultured Perkinsus marinus: Preliminary comparisons of thee inoculation methods. J Shellfish Res 1997; 16: 479-485.
25 Bettencourt R, Dando P, Collins P, Costa V, Allam B, Serrao Santos R. Innate immunity in the deep sea hydrothermal vent mussel Bathymodiolus azoricus. Comp Biochem Physiol A Mol Integr Physiol 2009; 152: 278-289.   DOI   ScienceOn
26 Goedken M, DeGuise S. Flow cytometry as a tool to quantify oyster defence mechanisms. Fish Shellfish Immun 2004; 16: 539-552.   DOI   ScienceOn
27 Soudant P, Paillard C, Choquet G, Lambert C, Reid HI, Marhic A, Donaghy L, Birkbeck TH. Impact of season and rearing site on the physiological and immunological parameters of the Manila clam Venerupis (=Tapes, =Ruditapes) philippinarum. Aquaculture 2004; 229: 401-418.   DOI   ScienceOn
28 Allam B, Paillard C, Auffret M, Ford SE. Effects of the pathogenic Vibrio tapetis on defence factors of susceptible and non-susceptible bivalve species. II. Cellular and biochemical changes following in vivo challenge. Fish Shellfish Immun 2006; 20: 384-397.   DOI   ScienceOn
29 Tiscar PG, Mosca F. Defense mechanisms in farmed marine molluscs. Vet Res Comm 2004; 28: 57-62.
30 Anderson RS, Beaven AE. Antibacterial activities of oyster (Crass-ostrea virginica) and mussel (Mytilus edulis and Geukensia demissa) plasma. Aquat Living Resources 2001; 14: 343-349.   DOI   ScienceOn
31 Sunila I, La Banca J. Apoptosis in the pathogenesis of infectious diseases of the Eastern oyster, Crassostrea virginica. Dis Aquat Org 2003; 56: 163-170.
32 Gagnaire B, Soletchnik P, Faury N, Kerdudou N, Le Moine O, Renault, T. Analysis of hemocyte parameters in Pacific oysters, Crassostrea gigas, reared in the field - Comparison of hatchery diploids and diploids from natural beds. Aquaculture 2007; 264: 449-456.   DOI