• Title/Summary/Keyword: Torulopsis

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Studies on the Yeasts for the Brewing of Soy sauce ( I ) -Isolation, identification and classification of the Yeasts in the soy sauce koji- (간장발효에 관여하는 효모에 관한 연구(제 1보) -제국중에 생육하는 효모에 대하여-)

  • Lee, Taik-Soo;Lee, Suk-Kun
    • Applied Biological Chemistry
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    • v.13 no.1
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    • pp.97-103
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    • 1970
  • This experiment was carried out to investigate the yeasts for the brewing of soy sauce. The yeasts in the soy sauce koji were isolated and identified. And they were classified by coloring with the treatment of TTC(2, 3, 5 triphenyltetrazolium chloride) agar and counted. The result, obtained was as follows; a) The number of the yeasts in the Koji was increased in process of incubation time: about $97{\times}10^3$ per gram of Koji incubated for 3 days, $135{\times}10^3$ 4 days and $179{\times}10^3$ 5days. b) They were classified as 53.6-71.9 per cent of TTC while yeasts for the most, 5.6-11.1 per cent of red yeasts, 6.8-19.5 per cent of red pink yeasts and 11.1-22.6 per cent of pink yeasts. c) 30 strains of yeasts were isolated from the Koji optionally, and they were identified as 6 genera and 11 species: 5 strains of Saccharomyces rouxii, 2 strains of Saccharomyces fermentati, 1 strain of Saccharomyces rosei, 6 strains of Saccharomyces rosei, 6 strains of Hansenula anomala, 1 strain of Hansenula suaveolens, 6 strains of Pichia polymorpha, 2 strains of Debaryomyces hansenii, 2 strains of Debaryomyces nicotianae, 2 strains of Torulopsis candida 2 strains of Torulopsis sake and 1 strains of Torulopsis sake and 1 strain of Candida pelliculosa. d) The yeasts belonging to Hansenula suaveolens, Hansenula anomala Candida pelliculosa, Debaryomyces nicotianae and Pichia polymorplza were classified as TTC white yeasts, Saccharomyces rouxii and Sacharomyces rosei were red, Saccharomyces fermentati and Debaryomyces hancenii were red pink, and Torulopsis candida and Torulopsis sake were pink. e) Generally the growth of the yeasts isolated were rapid on the media containing none or 10 per cent of sodium chloride but almost restrained on the media containing 15-18 per cent of sodium chloride.

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Studies on the Yeasts for the Brewing of Soy Sauce(2) -Isolation, identification and classification of the yeasts in the soy sauce mash- (간장발효에 관여하는 효모에 관한 연구 (제2보) -간장 덧 중에 생육하는 효모에 대하여-)

  • Lee, Taik-Soo;Lee, Suk-Kun;Shin, Bo-Kyu
    • Applied Biological Chemistry
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    • v.13 no.2
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    • pp.171-180
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    • 1970
  • The yeasts in the soy sauce mash were isolated and identified, and they were classified by coloring with the treatment of TTC(2, 3, 5, triphenyltetrazolium chloride) agar and counted in process of time. The results obtained were as follows: a) The number of ordinary and osmophilic yeasts in 1 ml. of the soy sauce mash showed a tendency to be increased from the mashing to the mature stages and to decrease in the aging stages: $127{\times}10^3$ immediately after mashing, $83{\times}10^3$ 1 month after, $356{\times}10^3$ 3 months after, $1250{\times}10^3$ 6 months after and $65{\times}10^3$ 2 years after mashing in the case of ordinary yeasts, and 0 after mashing, $40{\times}10^3$ 1 month after, $81{\times}10^3$ 3 months after, $358{\times}10^3$ 6 month after and $23{\times}10^3$ 2 years after mashing in the case of osmophilic yeasts. b) 50 strains of yeasts were isolated from the soy sauce mash optionally in process of fermentation period, and they were identified as 7 genera and 18 species: 10 strains of Saccharomyces rouxii, 1 strain of Saccharomyces marxianus, 3 strains of Saccharomyces rosei, 1 strain of Saccharomyces fermentati, 6 strains of Saccharomyces mellis, 1 strain of Saccharomyces acidifaciens, 1 strain of Saccharomyces pastori, 3 strains of Pichia polymorpha, 2 strains of Hansenula anomala, 1 strain of Hansenula saturnus, 2 strains of Hansenula suaveolens, 5 strains of Nadsonia fulvescens, 8 strains of Debaryomyces hasenii, 1 strain of Debaryomyces nicotianae, 1 strain of Debaryomyces kloeckeri, 2 strains of Torulopsis sake, 1 strain of Torulopsis holmii and 1 strain of Candida pelliculasa. c) Distribution of yeasts according to the fermentation period was as follows: i) Saccharomyces rouxii, Saccharomyces marxianus, Saccharoymces rosei, Pichia polymorpha, Debaryomyces hansenii, Torulopsis sake, Candida pelliculosa, Debaryomyces nicotianae, Nadsonia fulvescens, Hansenula suaveolens and Hansenula saturnus were found in the early stages of fermentation. ii) Saccharomyces rouxii, Saccharomyces rosei, Saccharomyces fermentati, Saccharomyces mellis, Saccharomyces pastori, Hansenula anomala, Saccharomyces acidifaciens and Debaryomyces hansenii appeared in the mature stages. iii) Saccharomyces rouxii, Saccharomyces mellis, Nadsonia fulvescenes, Dedaryomyces hansenii, Debaryomyces kloeckeri, Torulopsis sake and Torulopsis holmii were distributed in the aging stages. d) TTC white yeasts were found in abundance in the early stages of fermentation and TTC red yeasts appeared more than 50 per cent in the mature and aging stages. e) The yeasts belonging to Saccharomyces mellis and Saccharomyces pastori were classified as TTC red yeasts, Saccharomyces acidifaciens were reel pink, Hansenula saturnus Debaryomyces kloeckeri, and Torulopsis holmii were pink, Saccharomyces marxianus and Nadsonia fulvescens were white and the others were the same as the description in the previous report. Saccharomyces rouxii ware classified for the most part as TTC red yeasts, and while some of them were red pink. f) Species of yeasts in the soy sauce mash were similar to those in the soy sauce koji, but the latter were not osmophilic and in the former case, the osmophilic yeasts were increased in process of fermentation period.

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Preparation of Sophorose-II. Preparation of Sophorose from the Culture Broth of Torulopsis bombicola and the Pod of Sophora japonica (Sophorose의 제조-II. 효모(Torulopsis bombicola) 배양액 및 회화나무(Sophora japonica)로부터 Sophorose의 제조)

  • Lee, Mi-Ja;Lee, You-Hui;Park, Jong-Dae;Kim, Hae-Yeong;Kim, Shin-Il;Baek, Nam-In
    • Applied Biological Chemistry
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    • v.40 no.2
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    • pp.163-166
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    • 1997
  • A yeast, Torulopsis bombicola, was cultured in the media fortified with soybean oil as a additional carbon source for 7 days with reciprocal shaking. From the culture broth, sophorose-lipid was isolated and treated with alkali to afford sophorose. The sophorose contained in the medium was acetylated and isolated through silica gel column chromatography. The aceylated sophorose was hydrolyzed with 5% KOH at room temperature to give rise to sophorose. Meanwhile, the MeOH extracts obtained from the pod of Sophora japonica was solvent-fractionated with n-BuOH and $H_2O$, and butanolic layer was chromatographed on silica gel column to afford a flavonoide-glycoside. The glycoside was hydrolyzed with 0.02 N $H_2SO_4$ to yield sophorose.

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Studies on the wild yeasts in Korea (II) (한국산 야생효모에 관한 연구 2)

  • 박명삼;라철호
    • Korean Journal of Microbiology
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    • v.8 no.3
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    • pp.95-102
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    • 1970
  • From the crops Drosophila collected in Mt. Sokni and Mt.Kyeryong, 7 strains were isolated and then 6 species of wild yeast were identified. 1) Of these six species of wild yeasts two were to be of genus Saccharomyces(Ascosporgenous), two Torulopsis and two Trichosporon (both genuses of Asporogenous). 2) It was found that the fermentation of the wild yeasts isolated from Drosophila was much better than that of any others ; in particular, S. florentinus and S. cerevisiae were good in fermenting maltose. 3) After being cultivated in malt extract agar medium at $25^{\circ}C$ for 3 days, the vegetative cells were found to be big but Torulopsis cells small. 4) It was also observed that the species of yeasts used fro food by Drosophila largely depends on genus and species of Drophila. 5) Of the yeasts isolated from the Drosophila, Trichosporon capitatum and Torulopsis dattila, which has not previously been recorded, were identified. 6) It is believed, therfore, that S.florentinus, powerful in fermenting maltose, will be extremely useful in terms of industrial application.

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Development of a Novel Yeast Strain Which Ferments Soy Sauce by Protoplast Fusion

  • Lee, Eun-Ju;Kim, Jong-Kyu
    • Journal of Microbiology and Biotechnology
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    • v.3 no.1
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    • pp.24-30
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    • 1993
  • In order to develop a novel yeast which produces the charateristic aroma of soy sauce, a protoplast fusion between Zygosaccharomyces rouxii WFS4 and Torulopsis versatilis IAM 4993 was carried out. Auxotrophic mutants as selective markers were obtained from Zygosaccharomyces rouxii and Torulopsis versatilis by treatment of N-methyl-N -nitro-N-nitrosoguanidine. The conditions of the protoplast formation and the regeneration for fusion were examined. The protoplast fusion using polyethylene glycol 4000 led to the fusion frequency of $4~5{\times}10^{-7}\;cells/ml$. Among fusants, a fusant ST723-F31 presented the best results in terms of the aromaticity of fragrance, the growth pattern, the resistance against salt and the degree of growth according to pH. It makes easy to control the production and the balance of aroma components so that it gives a good flavor, shortens the fermentation period and, simplifies the preparation process when using a bioreactor into which fusant is immobilized.

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Causes of Can Swelling of Commercially Canned Fruits (과일 통조림의 팽창원인에 관한 연구)

  • Moon, Eun-Joo;Kim, Hyeung-Yong;Kyung, Kyu-Hang
    • Korean Journal of Food Science and Technology
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    • v.17 no.2
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    • pp.89-94
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    • 1985
  • Seventy nine swollen commercially canned grapes and peaches were collected from wholesale and retail outlets in Seoul area for the investigation of the causes of their swell. Hydrogen generation and microbial spoilage were the only two common causes of can swelling. Nevertheless, the spoilage profile of the two products was different. Seventeen out of twenty one canned peaches were spoiled by microorganisms, while the rest of five were swollen due to hydrogen generation. In contrast, fifty one out of fifty eight canned grapes were swollen due to hydrogen generation, while the rest of seven were spoiled by microorganisms. Seventeen yeast and five bacterial strains were isolated and only the yeasts were identified to their genera and species. Saccharomyces cerevisiae was the most frequently isolated spoilage yeast, closely followed by Torulopsis stellata. Candida parapsilosis, Pichia kluyveri, Pichia membranaefaciens, Torulopsis globosa and Torulopsis lactis-condensi were also isolated but with lower frequencies.

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Growth Profile of Some Yeasts in Pear Marc Extracts (배 추출박 추출물에서 몇 종류 효모들의 생육 특성)

  • Jang, In-Taek;Kang, Min-Gu;Na, Kwang-Chul;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.39 no.3
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    • pp.229-230
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    • 2011
  • Growth profiles of Candida krusei KCTC 7213, Torulopsis sphaerica KCTC 7138 and Zygosaccharomyces rouxii KCCM 12066 in pear marc extract were determined. Candida krusei KCTC 7213 showed the highest growth in 20 hr cultivation at $30^{\circ}C$, whereas both of Torulopsis sphaerica KCTC 7138 and Zygosaccharomyces rouxii KCCM 12066 established maximal growth by 25 hr of cultivation at $30^{\circ}C$ in pear marc extract. However, all of yeasts showed lower growth in pear marc extracts rather than those of YEPD medium.

Screening for Antioxidative Activities of Yeasts on Fish Oil (어유에 대한 효모와 항산화효과)

  • 류병호;김혜성;정종순;이상훈;지영애
    • Journal of Food Hygiene and Safety
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    • v.2 no.1
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    • pp.15-20
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    • 1987
  • A general screening test for the expression of antioxidative activity was performed on over 36 cultures belong to yeast isolated from soy sauce, Makkuli, and molasses. Antioxidative activities of yeasts were examined by measuring oxidation such as peroxide value and thiobarbituric acid value in fish oil. Of these cultures, Saccharomyces cerevisiae IFO 2114 were found to have strong antioxidative activity. Saccharomyces rouxii and Torulopsis etchellsii isolated from soy sauce showed the strongest antioxidative activity among yeasts. Pichia ohmerii isolated from Makkuli showed the strongest antioxidative activity and Candida versatilis isolated from molasses showed also relative strong antioxidative activity.

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The Distribution and Population Densities of Yeasts and their Epiflora on Seaweeds in Inshore Waters of Mok-po, Korea (한국 목포 연해안해수내의 효모 분포 및 집단밀도와 해조류상의 효모상)

  • 전순배;박명삼
    • Korean Journal of Microbiology
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    • v.13 no.4
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    • pp.157-164
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    • 1975
  • The distribution and population densities of yeasts including their epiflora on seaweeds were estimated over two months in inshore waters of Mok-po, Korea. Nine species of 48 isolates were obtained from this area. Rhodotorula, Torulopsis and Debaryomyces that are widespread in estuaries were of common occurrence in this water body. The highest counts belonged to Torulopsis candida which was predominated in temperate estuarine zone. The distribution of sea weeds seems to be correlated with the population densities of yeasts. Two species of marine algae harbored yeasts during May and July, 1975. Among seaweeds isolates, the highest numbers werre of the species Rhodotorula glutinis var. glutinis. A dominant colonization of this strain on Chlorophyceae and Phaeophyta and a role of water temperature for the growth of yeasts are discussed.

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Studies on Yeasts Isolated from Marine Substrates (I) (해양효모의 분류 1)

  • 전순배
    • Korean Journal of Microbiology
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    • v.8 no.4
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    • pp.141-150
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    • 1970
  • As a part of taxonomical and ecological studies on the yeasts in marine environments, several kinds of yeasts were isolated from Zostera marina several invertebrates (penaeus, Meretrix and Neptunus) and surface sea water, which are collected at the two established sites of estuarine areas ; Dolsan isaland in Youchun district and Baiksu-ri in Youngkwang district. The obtained results can be summarized as follows. 1. Ascosporgenous Yeasts. Hansenula sp I and Hansenula sp II were isolated from Zotera marina and Hanse nular sp I from penaeus. 2. Asporogenous Yeasts. Trichoporon fermentans, Torulopsis, ernobii and Toruopsis dattia were isolated from Zostera marina, Candida krusei from Meretrix and Neptunus, Torulopsis pinus from surface sea waters, and Phodotorula aurantiaca from Penaeus. 3. More notable isolations of several species from Zostera marina than the other sources could be assumed as related to the higher sugar concentration of this plant.

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