• The Korea Journal of Herbology
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• v.28 no.6
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• pp.9-14
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• 2013

Objectives : Cnidii Fructus is prescribed as the fruit of Cnidium monnieri (L.) Cusson or Torilis japonica (Houtt.) DC. in Korea pharmacopoeia. Although there are differences in the composition of useful components, two species have been used without distinction. In order to discriminate them, DNA sequencing and taste pattern analysis were used in this study. Methods : Primers ITS 1 and ITS 4 were used to amplify the intergenic transcribed spacer(ITS) region of nuclear ribosomal DNA from seven T. japonica and six C. monnieri samples. Taste pattern of samples were measured by using taste-sensing system SA402B equipped with five foodstuff sensors(CT0, C00, AAE, CA0, and AE1). The five initial taste(sourness, bitterness, astringency, umami, and saltiness) and three aftertaste(aftertaste of bitterness, astringency, and umami) of two species were compared. Results : According to the results of ITS region sequence analysis, two species showed 94 base pairs differences. The similarity of two sequences was 85%. From the taste pattern analysis, sourness, bitterness, aftertaste of bitterness(aftertaste-B), and umami showed a different pattern. Especially, bitterness and aftertaste-B of C. monnieri were significantly higher than T. japonica. In addition, two species were shown to have two markedly different clustering by these two flavors. Conclusion : T. japonica and C. monnieri were effectively discriminated using DNA sequencing and taste pattern analysis. These methods can be used to identify the origin of traditional medicine in order to maintain therapeutic efficacy.

• Korean Journal of Pharmacognosy
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• v.30 no.2
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• pp.137-144
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• 1999

Torilin was isolated from haxane fraction of Torilis Fructus extract. Torilin produced inhibition of the acetic acid-induced and phenylquinone-induced writhing syndrome at the oral doses of 30 and 90 mg/kg in mice. It also increased the pain threshold at the oral doses of 30, 90 and 270 mg/kg in the tail pressure method and the Randall-Selitto method. However, it did not show a hypothermic action at the oral doses of 30 and 90 mg/kg in mice. The compound exhibited strong anticarrageenan activity at the oral doses of 90 and 270 mg/kg in rats, and had inhibitory effect on the vascular permeability at the oral doses of 30 and 90 mg/kg in mice. It also showed potent inhibition of leucocyte emigration in CMC-pouch at the doses of 3 and 9 mg/rat, sc. The acute toxicity of torilin was very weak: the $LD_{50}$ values were more than 5000 mg/kg, po and 2000 mg/kg, ip in mice. From the above mentioned results, it was suggested that torilin had potent analgesic and anti-inflammatory activities.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.13 no.1
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• pp.267-279
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• 2000

Minimum inhibitory concentration(MIC) of Sophorae Subprostratae Radix. Aconiti Radix, Hibisci Syriaci Cortex and those mixture. Phellodendri Cortex, Sophorae Radix, Torilis Fructuson on various species of the dermatophytes on Microsporium gypseum, Trichophytone rubrum and Epidermophytone floccosum were determined after 4 weeks of incubation at $25^{\circ}C$ in Sabouraud dextrose broth. The results were summarized as followes : 1. Antifungal effects of Phellodendri $Cortex\;{\cdot}Hibisci$ Syriaci Cortex and mixture of Sophorae Subprostratae Radix, Aconiti Radix, Hibisci Syriaci Cortex on T rubrum which is the most common organism of tinea pedis were more effective than that of the others. 2 These results suggested that herbal medicines such as Phellodendri $Cortex\;{\cdot}Hibisci$ Syriaci Cortex and mixture of Sophorae Subprostratae Radix, Aconiti Radix, Hibisci Syriaci Cortexse can be used to treat tinea pedis and to improve untoward effects associated with the administration of antifugual agents.

• Korean Journal of Medicinal Crop Science
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• v.23 no.5
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• pp.400-405
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• 2015

Background : As the characterization of medicinal plants is an important aspect of traditional Asian herbal medicine, this study examines the morphological and anatomical characteristics in four fructus form medicinally important plants belonging to the family Apiaceae. Methods and Results : Fruit morphology of four genera was examined using microtome sections and light microscopy. The Anethi Fructus, Anethum graveolens (Siraja) has unique wing-like and membranous lateral ribs that are, approximately $500{\mu}m$ wide. The Coriandri Fructus, Coriandrum sativum (Hoyuja) does not have oil ducts at the dorsal region of the mericarp and differs in the development of the dorsal ribs. The ribs appear upon drying and therefore, consist of primary and secondary ribs. The Foeniculi Fructus, Foeniculum vulgare (Hoehyang) characteristically develops three dominant dorsal ribs and has a strong aromatic fragrance. Finally, the Cnidii Fructus, Torilis japonica (Sasangja) has many oil ducts at the dorsal and carpophores regions and develops many hooked trichomes one the fructus surface. Conclusions : We conclude from this study that each plant has several prominent and distinguishing morphological and anatomical characteristics. Therefore, fruit morphology is very useful for identifying these medicinal plants. In addition, the correct use and herbal name need to be standardized for plants used traditionally in Asian medicine within Korea, China, and Japan.

• Journal of Haehwa Medicine
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• v.9 no.1
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• pp.319-335
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• 2000

According to the literatual study on the external medical trearment of Menorrhalgia and Leucorrhea for gynecologic condition, the results were as follows. 1. Using external medical treatment for Menorrhalgia is to insert vagina theraphy, fumigation theraphy, to wash vagina theraphy, to insert anus theraphy, to apply hot pack theraphy and use with suppository such as BANSUKHWAN(礬石丸), SASANGZASAN(蛇床子散), KUMBONGHYUNGJU(金鳳衡珠) 2. Using external medical treatment for Leucorrhea is washing and fumigation on vagina, to wash vagina, to insert vagina, cleansing theraphy and use with Suppository such as YONGYEOMGO(龍鹽膏), KAMISASANGJASAN(加味蛇床子散), SASANGSACHUNGSEJE(蛇床子洗劑). 3. ANGELICAE GIGANTIS RADIX (當歸) is in mostly general use for external medical treatment of Menorrhalgia and Leucorrhea, in that order ANSU SEMEN(杏仁), EVODIAE FRUCTUS(吳茱萸), TORILIS FRUCTUS (蛇床子), CORYDALIS TUBER(玄胡索), CINIAMOMI CORTEX(肉桂), CARYOPHYLLI FLOS(丁香), ALUMEN(枯白礬), AQUILARIAE LIGNUM (沈香). 4. The efficacy of medicines to use external medical treatment is as follow to help circulation of blood, to warm spleen and stomach, to warm blood, to warm uterus and remove cold, to remove heat and dry moisture, to down heat-product, to contract bloodvessel, to counteract poison and destory virus, to make energy and blood. 5. KUMBONGHYUNGJU, made same size as cherry and shape vagina medicine, use for Menorrhalgia, menstural irregularity, Leucorrhea, it has the efficacy as follow to remove cold and dry moisture, to warm uterus, to help circulation and romove pain, to down moisture-heat.

• Journal of Ginseng Research
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• v.31 no.4
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• pp.196-202
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• 2007

Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-I (MMP-1) activity. This study was carried out to select optimum ratio of 3 herbs in skin health food for anti-wrinkle. Human dermal fibroblast cell was incubated with experimental samples, which were Korean red ginseng ethanol extracts (ER), Torilis fructus water extracts (WT), Corni fructus water extracts (WC) and their mixtures (WM1, WM3). And then we determined effects on collagen biosynthesis, MMP-1 activity and SOD activity in human dermal fibroblast cell. In control group, collagen biosynthesis was amounted at 474.8 ng/ml and 533.9 ng/ml, 539.3 ng/ml, 514.1 ng/ml in ER, WT and WC respectively. Furthermore, WM3 (KTNG0345) was increased to 561.45 ng/ml. MMP-1 activity of ER, WT, WC, WM1 were determined to 31.9 ng/ml, 32.85 ng/ml, 32.0 ng/ml, 31.3 ng/ml and WM3 (KTNG0345) was decreased to 28.85 ng/ml. In addition, the experimental samples showed a antioxidative activities. From this results, we conclude that Korean red ginseng ethanol extracts, Torilis fructus water extracts, Corni fructus water extracts and their mixtures have a anti-wrinkle effect and WM3 (KTNG0345) may be regarded as an optimum composition for synergic effect producing. The standardized components of KTNG0345, ginsenoside-$Rb_1$, torilin and loganin were identified at 10.85 mg/g, 0.128 mg/g and 3.92 mg/g respectively.

• Applied Biological Chemistry
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• v.44 no.1
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• pp.20-23
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• 2001

Several unusual fatty acids including myristic acid (14 : 0) and lauric acid (12 : 0) were investigated in the Iris family and other related plants. Especially the roots of Iris tecorum contained 75.9% myristic acid in total fatty acid contents and that of Iris germanica contained 57% myristic and 15.5% lauric acid (12 : 0) whereas 10.7% lauric acid and 9.5% capric acid (10 : 0) were detected in the roots of iris ensata as compared to the total fatty acid contents. The total fatty acid contents in the seeds of Foeniculi fructus and Torilis japonica was relatively higher 193.3 mg/g dry wt and 128.2 mg/g dry wt, respectively. 64.5% linoleic acid (18 : 2) and 48.9% ${\alpha}-linolenic$ acid (18 : 3) were observed in the seeds and leaves of iris tectorum whereas its lateral roots contained 9.5% caprylic acid (8 : 0) and 8.6% capric acid. The percentage of myristic acid of the total fatty acid in the immature seeds of iris tectorum and Iris germanica was 10.8% and 15.6%, respectively.

• Journal of Ginseng Research
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• v.32 no.1
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• pp.39-47
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• 2008

UV irradiation causes skin-aging involving coarse wrinkles, thickening, dyspigmentation, and rough skin surface. These phenomena in complex skin tissue is controlled with receptor of cell surface growth factor and cytokine receptors. The activation of receptors induces multiple downstream signaling pathways including expression of MMPs (matrix metalloproteinases). This study was aimed to elucidate the mechanism for anti-wrinkle activity of Korean red ginseng, Torilis fructus and Corni fructus mixture (KTNG0345). In this animal study, we have investigated decreasing effects of Korean red ginseng mixture on MMP-3 synthesis through diminishing $TNF-{\alpha}$ signaling that express MMP-1, -3, and -9. c-Jun and c-fos as a component of transcription factor AP-1 (activator protein-1) were analyzed the expression level using real time PCR and western blotting. c-Jun was decreased dose dependent manner both gene and protein level where as cfos was not changed. In upstream, JNK and PAK was not changed, but p38 was decreased in down stream. MMP-3, final product in this pathway was significantly decreased in dose dependent manner. These results suggest that Korean red ginseng mixture have a anti-wrinkle activity through $TNF-{\alpha}$ mediated MMPs expression pathway.