• 대한본초학회지
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• 제28권6호
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• pp.9-14
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• 2013

Objectives : Cnidii Fructus is prescribed as the fruit of Cnidium monnieri (L.) Cusson or Torilis japonica (Houtt.) DC. in Korea pharmacopoeia. Although there are differences in the composition of useful components, two species have been used without distinction. In order to discriminate them, DNA sequencing and taste pattern analysis were used in this study. Methods : Primers ITS 1 and ITS 4 were used to amplify the intergenic transcribed spacer(ITS) region of nuclear ribosomal DNA from seven T. japonica and six C. monnieri samples. Taste pattern of samples were measured by using taste-sensing system SA402B equipped with five foodstuff sensors(CT0, C00, AAE, CA0, and AE1). The five initial taste(sourness, bitterness, astringency, umami, and saltiness) and three aftertaste(aftertaste of bitterness, astringency, and umami) of two species were compared. Results : According to the results of ITS region sequence analysis, two species showed 94 base pairs differences. The similarity of two sequences was 85%. From the taste pattern analysis, sourness, bitterness, aftertaste of bitterness(aftertaste-B), and umami showed a different pattern. Especially, bitterness and aftertaste-B of C. monnieri were significantly higher than T. japonica. In addition, two species were shown to have two markedly different clustering by these two flavors. Conclusion : T. japonica and C. monnieri were effectively discriminated using DNA sequencing and taste pattern analysis. These methods can be used to identify the origin of traditional medicine in order to maintain therapeutic efficacy.

• 생약학회지
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• 제30권2호
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• pp.137-144
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• 1999

Torilin was isolated from haxane fraction of Torilis Fructus extract. Torilin produced inhibition of the acetic acid-induced and phenylquinone-induced writhing syndrome at the oral doses of 30 and 90 mg/kg in mice. It also increased the pain threshold at the oral doses of 30, 90 and 270 mg/kg in the tail pressure method and the Randall-Selitto method. However, it did not show a hypothermic action at the oral doses of 30 and 90 mg/kg in mice. The compound exhibited strong anticarrageenan activity at the oral doses of 90 and 270 mg/kg in rats, and had inhibitory effect on the vascular permeability at the oral doses of 30 and 90 mg/kg in mice. It also showed potent inhibition of leucocyte emigration in CMC-pouch at the doses of 3 and 9 mg/rat, sc. The acute toxicity of torilin was very weak: the $LD_{50}$ values were more than 5000 mg/kg, po and 2000 mg/kg, ip in mice. From the above mentioned results, it was suggested that torilin had potent analgesic and anti-inflammatory activities.

• 한방안이비인후피부과학회지
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• 제13권1호
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• pp.267-279
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• 2000

Minimum inhibitory concentration(MIC) of Sophorae Subprostratae Radix. Aconiti Radix, Hibisci Syriaci Cortex and those mixture. Phellodendri Cortex, Sophorae Radix, Torilis Fructuson on various species of the dermatophytes on Microsporium gypseum, Trichophytone rubrum and Epidermophytone floccosum were determined after 4 weeks of incubation at $25^{\circ}C$ in Sabouraud dextrose broth. The results were summarized as followes : 1. Antifungal effects of Phellodendri $Cortex\;{\cdot}Hibisci$ Syriaci Cortex and mixture of Sophorae Subprostratae Radix, Aconiti Radix, Hibisci Syriaci Cortex on T rubrum which is the most common organism of tinea pedis were more effective than that of the others. 2 These results suggested that herbal medicines such as Phellodendri $Cortex\;{\cdot}Hibisci$ Syriaci Cortex and mixture of Sophorae Subprostratae Radix, Aconiti Radix, Hibisci Syriaci Cortexse can be used to treat tinea pedis and to improve untoward effects associated with the administration of antifugual agents.

• 한국약용작물학회지
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• 제23권5호
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• pp.400-405
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• 2015

Background : As the characterization of medicinal plants is an important aspect of traditional Asian herbal medicine, this study examines the morphological and anatomical characteristics in four fructus form medicinally important plants belonging to the family Apiaceae. Methods and Results : Fruit morphology of four genera was examined using microtome sections and light microscopy. The Anethi Fructus, Anethum graveolens (Siraja) has unique wing-like and membranous lateral ribs that are, approximately $500{\mu}m$ wide. The Coriandri Fructus, Coriandrum sativum (Hoyuja) does not have oil ducts at the dorsal region of the mericarp and differs in the development of the dorsal ribs. The ribs appear upon drying and therefore, consist of primary and secondary ribs. The Foeniculi Fructus, Foeniculum vulgare (Hoehyang) characteristically develops three dominant dorsal ribs and has a strong aromatic fragrance. Finally, the Cnidii Fructus, Torilis japonica (Sasangja) has many oil ducts at the dorsal and carpophores regions and develops many hooked trichomes one the fructus surface. Conclusions : We conclude from this study that each plant has several prominent and distinguishing morphological and anatomical characteristics. Therefore, fruit morphology is very useful for identifying these medicinal plants. In addition, the correct use and herbal name need to be standardized for plants used traditionally in Asian medicine within Korea, China, and Japan.

• 혜화의학회지
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• 제9권1호
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• pp.319-335
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• 2000

According to the literatual study on the external medical trearment of Menorrhalgia and Leucorrhea for gynecologic condition, the results were as follows. 1. Using external medical treatment for Menorrhalgia is to insert vagina theraphy, fumigation theraphy, to wash vagina theraphy, to insert anus theraphy, to apply hot pack theraphy and use with suppository such as BANSUKHWAN(礬石丸), SASANGZASAN(蛇床子散), KUMBONGHYUNGJU(金鳳衡珠) 2. Using external medical treatment for Leucorrhea is washing and fumigation on vagina, to wash vagina, to insert vagina, cleansing theraphy and use with Suppository such as YONGYEOMGO(龍鹽膏), KAMISASANGJASAN(加味蛇床子散), SASANGSACHUNGSEJE(蛇床子洗劑). 3. ANGELICAE GIGANTIS RADIX (當歸) is in mostly general use for external medical treatment of Menorrhalgia and Leucorrhea, in that order ANSU SEMEN(杏仁), EVODIAE FRUCTUS(吳茱萸), TORILIS FRUCTUS (蛇床子), CORYDALIS TUBER(玄胡索), CINIAMOMI CORTEX(肉桂), CARYOPHYLLI FLOS(丁香), ALUMEN(枯白礬), AQUILARIAE LIGNUM (沈香). 4. The efficacy of medicines to use external medical treatment is as follow to help circulation of blood, to warm spleen and stomach, to warm blood, to warm uterus and remove cold, to remove heat and dry moisture, to down heat-product, to contract bloodvessel, to counteract poison and destory virus, to make energy and blood. 5. KUMBONGHYUNGJU, made same size as cherry and shape vagina medicine, use for Menorrhalgia, menstural irregularity, Leucorrhea, it has the efficacy as follow to remove cold and dry moisture, to warm uterus, to help circulation and romove pain, to down moisture-heat.

• Journal of Ginseng Research
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• 제31권4호
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• pp.196-202
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• 2007

본 실험은 홍삼, 사상자, 산수유를 이용하여 주름 예방 효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 개별추출물과 이들의 혼합물 WM1, WM3 (KTNG0345)에 대하여 사람의 섬유아세포를 이용한 세포증식, 콜라겐 생합성 정도, MMP-1 효소활성, 항산화 활성을 조사하였다. 사용된 시료는 $1{\sim}10\;{\mu}g/ml$의 농도로 첨가하여 세포를 배양했을 때 $83{\sim}100%$의 세포증식율을 나타내어 세포 독성을 나타내지 않았으며, 콜라겐 생합성 정도는 대조군이 474.8 ng/ml이었고 retinoic acid는 568.3 ng/ml이었으며, 5 ug/ml의 농도를 기준으로 할 때 홍삼은 533.9ng/ml, 사상자는 539.3 ng/ml, 산수유는 514.1 ng/ml이었다. 홍삼과 생약을 68:232의 비율로 혼합한 WM1은 474.6 ng/ml이었고 홍삼과 생약을 136:164의 비율로 혼합한 WM3는 561.4 ng/ml로 증가하였다. MMP-1활성은 10 ug/ml의 농도에서 홍삼(ER)은 31.9 ng/ml, 사상자(WT)는 32.85 ng/ml, 산수유(WC)는 32.0 ng/ml, WM1은 31.3 ng/ml이었고 WM3(KTNG0345)는 28.85 ng/ml으로 대조군의 88% 수준으로 감소하였다. SOD활성에서는 1000 ug/ml의 농도로 처리하였을 때 65-97% 항산화활성을 나타내었다. 이들의 결과를 종합해 보면 홍삼과 사상자, 산수유 및 이들 혼합물은 human dermal fibroblas에 대하여 세포 독성을 나타내지 않으면서 콜라겐 생합성을 촉진하고 MMP-1의 활성을 억제하였으며 홍삼과 생약 엑기스를 136:164의 비율로 혼합했을 때 그 효과가 증진되었다. WM3 (KTNG0345)는 지표성분으로서 ginsenoside-$Rb_1$, torilin, loganin을 각각 10.85 mg/g, 0.128 mg/g, 3.92 mg/g 함유하였다.

• Applied Biological Chemistry
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• 제44권1호
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• pp.20-23
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• 2001

산업적으로 활용성이 높은 특이 지방산을 탐색하기 위하여 8종의 국내 자원식물을 재료로 하여 지방산 조성을 조사한 결과 붓꽃(Iris tectorum) 등 3가지 식물에서 myristic acid(14 : 0)와 lauric acid(12 : 0)등의 중쇄지방산이 함유되어 있음을 확인하였다. 특히 붓꽃에는 myristic acid가 전체 지방산의 75.9%를 차지하였다. 독일붓꽃(Iris germanica)에는 myristic acid와 lauric acid가 전체 지방산의 57%와 15.5% 씩 함유되어 있었다. 꽃창포(Iris ensata) 뿌리에는 lauric acid와 capric acid(10 : 0)가 각각 10.7%와 9.5% 함유되어있었으나 회향(Foeniculum vulgare)과 사상자(Torilis Japonica)의 종실에는 전체 지방산(mg/g dry wt)이 각각 193.3 mg/g과 128.2 mg/g의 높은 함량을 나타내었다. 붓꽃의 부위별 지방산 조성에서는 종실에 linoleic acid(18 : 2)가 64.5%, 잎에 ${\alpha}-linolenic$ acid(18 : 3)가 48.9% 그리고 곁뿌리(lateral root)에는 caprylic acid(8 : 0)와 capric acid가 각각 9.5%와 8.6%씩 함유되어 있었으며 붓꽃과 독일붓꽃의 미숙종자에는 myristic acid가 각각 10.8%와 15.6% 함유되었다.

• Journal of Ginseng Research
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• 제32권1호
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• pp.39-47
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• 2008

본 실험은 홍삼 혼합물 (KTNG0345)을 이용한 주름 예방 및 개선효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 시료를 경구투여한 무모생쥐의 피부조직 으로부터 MMP-3의 발현양상과 작용 메커니즘을 연구하였다. MMP-3의 발현정도는 농도 의존적으로 현저한 감소를 나타내었으며, 유전자와 단백질 모두에서 동일한 양상을 보였다. PAK는 변화가 없었지만, p38, p-p38 그리고 c-Jun, p-c-Jun 을 통계적으로 유의하게 감소시킴으로써 MMPs의 발현 감소를 가져온 것으로 보인다. 뿐만 아니라 자외선에 의한 $TNF-{\alpha}$의 생성 또는 유입을 억제함으로써 $TNF-{\alpha}$ receptor에 의해 매개되는 신호전달 경로를 둔화시켜 MMPs의 발현을 감소시킨 것으로 보인다. 이렇게 KTNG0345는 복합적인 활성으로 작용하여 주름생성 억제 활성을 보이는 것으로 판단된다.