• 제목/요약/키워드: Toothpick method

검색결과 12건 처리시간 0.035초

DNA Microarray Probe Preparation by Gel Isolation Nested PCR

  • Wang, Hong-Min;Ma, Wen-li;Huang, Hai;Xiao, Wei-Wei;Wang, Yan;Zheng, Wen-Ling
    • BMB Reports
    • /
    • 제37권3호
    • /
    • pp.356-361
    • /
    • 2004
  • To develop a simplified method that can rapidly prepare DNA microarray probes in a massive scale, a lambda phage genomic DNA-fragments library was constructed for the microarray-probes collection. Four methods of DNA band recovery from the first PCR products were tested and compared. The DNA microarray probes were collected by a novel method of nested PCR that was mediated by gel isolation of the first PCR products. This method was named GIN-PCR. The probes that were prepared by this GIN-PCR technique were used as subjects to fabricate a DNA microarray. The results showed that a wooden toothpick was superior to the other 3 methods, since this technique can steadily transfer the DNA bands as the template of the second PCR after the first PCR. A group of probes were successfully collected and DNA microarrays were constructed using these probes. Hybridization results demonstrated that this technique of DNA recovery and probe preparation was rapid, efficient, and effective. We developed a cost-effective and less labor-intensive method for DNA microarray probe preparation by nested PCR that is mediated by wooden toothpick transfer of the DNA bands in the gel after electrophoresis.

Toothpick method(와타나베법)을 이용한 장애인 구강건강관리 효과 (Effect of the tooth pick of the disabled person oral treatment)

  • 오하민;송경희
    • 대한치위생과학회지
    • /
    • 제1권1호
    • /
    • pp.37-46
    • /
    • 2018
  • This study conducted a 'specialist tooth brushing' method against the severely disabled once every other week. The purpose of this study is to analyze and validate the effectiveness of maintaining healthy periodontal management when consistent oral hygiene lasts with minimum stimuli for 24 - months. The conclusions were as follows. Table 2 compares the difference between group 1 and 2 from the 1st to 11th management and verifies the actual difference in measurement of each index. Group 1 used a professional tooth brush while Group 2 used a general brush. There were found many significant differences in dental index. As for PHP, Oral Malodor index and gingival bleeding, it was found that one or two times of dental care can make substantial differences in dental health condition. These results show that as the number of toothbrush method has been increased, gingival bleeding and periodontal index are significantly improved, not to mention the improvement of gingivitis and periodontal disease. This is because periodontal tissue is affected by brushing method, brushing time and consistency of dental care. During the 6-month period, 20 minutes of active periodontal care was intensively conducted, and for 18-months the effect of consistent care was verified by 10-minute periodontal care once every other week. As a result of the test, it was found that there was no complete regression in the basic periodontal treatment and the periodontal health condition had been maintained for 24 months. Also this test shows that, despite of inconsistent dental hygienic care, regular plague control can prevent dental diseases and maintain the dental health. This study proved that periodontal condition can be maintained by periodontal care once every other week as the tooth brushing properly stimulates the gums with positive effect. Therefore, professional dental healthcareworkers should be designated for each facility for the disabled, and dental health of the disabled should be professionally cared on a regular basis, and consistent and repetitive management by the dental care specialist are required.

전문가잇솔질에 의한 치주상태 변화와 환자만족도 (Change in Periodontal Status by Professional Toothbrushing, and Patients' Satisfaction)

  • 김창희;김진;김창욱
    • 치위생과학회지
    • /
    • 제8권4호
    • /
    • pp.261-266
    • /
    • 2008
  • 본 연구는 잇솔질 방법 중에 하나인 전문가 잇솔질법(Toothpick Method)을 이용하여 치주질환의 예방효과 및 환자들의 만족도를 파악하기 위하여 2008년 1월 9일부터 2월 28일까지 치주질환으로 치과의원에 내원한 환자 33명을 대상으로 전문가잇솔질법과 설문조사를 실시하였으며, SPSS WIN 12.0 프로그램을 이용하여 분석한 결과 다음과 같은 결론을 얻었다. 1. 치면세균막지수와 탐침시 출혈을 비교한 결과 치면 세균막지수에서는 최종검사를 시행한 후 초기치료직후와 비교한 결과 초기정밀검진 시 보다 0.16점으로 감소하였으며, 탐침 시 출혈은 2.96점으로 감소하여 통계적으로 유의한 차이를 보였다(p < .001). 2. 전문가잇솔질에 대한 환자만족도는 5점 만점에서 4.20으로 만족도가 높은 것으로 조사되었으며, 전반적인 프로그램 만족 항목에서 4.67로 가장 높게 조사되었으며, 치석제거와 비교 시 만족정도에서는 3.97로 가장 낮게 조사되었다. 일반적인 특성에 대한 환자만족도는 모두 통계적으로 유의한 차이는 없었다(p > 0.05). 3. 전문가 잇솔질 적용 후 성별에 따른 구강 내 자각인 지도를 살펴본 결과 '잇몸이 좋아졌다'가 남성의 경우 16.4%, 여성은 25.5%로 가장 높게 조사되었고, '출혈감소'는 남성은 14.5%, 여성은 10.9%로 나타났고, '입냄새 감소'는 남성이 10.9%, 여성이 7.3% 순으로 조사되었다. 전문가 잇솔질을 이용해 환자구강건강관리를 한 결과 치면세균막지수와 출혈율이 감소한 것으로 조사되었으며, 전문가잇솔질에 대한 환자만족도는 5점 만점에서 4.20으로 만족도가 비교적 높은 것으로 조사되었다. 위의 결과로 미루어 볼 때, 치과진료실 내에 환자의 치주건강을 유지할 수 있는 구강건강관리 인력의 요구와 교육이 필요하며 구강건강증진 프로그램 개발이 절실히 필요하다고 생각한다.

  • PDF

An Innovative Scalp-Dyeing Technique with Gentian Violet Solution During Follicular Unit Extraction for White-Haired Follicular Units

  • Moon, Min Seon;Choi, Jong Pil
    • Archives of Plastic Surgery
    • /
    • 제44권2호
    • /
    • pp.170-172
    • /
    • 2017
  • There exist some restrictions and difficulties in performing follicular unit extraction (FUE) in white-haired patients, for several reasons. In this paper, we introduce a novel technique for visualizing white hair during the punching procedure and graft preparation in FUE for white-haired patients. In white-haired older male patients, we dyed the surrounding scalp skin purple with a gentian violet solution-stained toothpick. Our method has several advantages: surgeons can easily focus on the center of the follicular unit and rapidly perform punching, they can recognize the condition of the harvested follicular units during FUE, and the hair transplant team can secure a clear view for trimming and loading into the implanter. We suggest that scalp dyeing in difficult FUE procedures, especially in patients with white hair, may be a simple method that provides a good visualization for donor site harvesting and for microdissection.

일부 서비스 종사자들의 구강위생보조용품의 사용실태에 관한 조사 연구 (A study on usage status of auxiliary oral hygiene devices in service workers behavior)

  • 김명은
    • 한국치위생학회지
    • /
    • 제8권3호
    • /
    • pp.37-52
    • /
    • 2008
  • 본 연구는 서비스 종사자들의 구강위생보조용품의 사용 실태를 조사하기 위해 서울시 S생명사와 L백화점에 종사하는 서비스군 200명과 사무직에 종사하는 비서비스군 200명을 대상으로 칫솔질 습관, 구강위생보조용품의 사용행태 및 만족도 등에 관한 면접설문조사를 실시하여 비교한 결과 다음과 같은 결론을 얻었다. 1. 서비스군의 양치 횟수는 하루 3~4회(53.4%)가, 양치방법은 위 아래로 닦는 방법(50.8%)이 가장 많았으며, 양치 시기는 식후 5분 후(45.0%)가 하는 경우가, 칫솔 교환 시기는 모양을 보고 교환하는 경우(56.6%)가 가장 많았다. 2. 서비스군은 비서비스군에 비해 구강위생보조용품을 많이 사용하고 있었고 그 사용 빈도는 이쑤시개(30.9%), 구강양치용액(29.9%), 치실(13.5%), 혀닦이(10.1%) 순이었으며 그 외의 구강위생보조용품의 사용 빈도는 매우 낮았다. 3. 각 구강위생보조용품 별로 두 군 간의 사용현황을 비교해 보면, 서비스군은 치실(66.1%),의 사용이 비서비스군(33.9%)에 비해 더 많았고, 혀닦이(64.4%), 역시 비서비스군(33.3%)보다 더 많이 사용하고 있었으며, 구강양치용액에서도 서비스군이 (64.43%) 비서비스군에(35.6%) 비해 더 많이 사용하고 있는 것으로 나타났다. 반면 이쑤시개에 있어서는 비서비스군(54.6%)이 서비스군(45.4%)에 비해 더 많이 사용하고 있는 것으로 나타났다 4. 서비스 종사자들의 구강위생보조용품의 사용동기는 치과에서 권유하여 사용하는 경우(53.6%)가 가장 많았고, 구강위생보조용품을 사용하지 않는 이유는 사용하기 불편하거나(45.4%), 사용하는 방법을 잘 모르기 때문(21.60%)이 가장 많은 것으로 조사되었다. 5. 구강위생보조용품의 인지도에 따른 사용률을 조사한 결과 이쑤시개를 제외한 대부분의 구강위생보조용품에서 서비스군이 비서비스군보다 높은 이용률을 보였으며 특히 구강양치용액(54.8%), 혀닦이(43.3%) 치실(35.8%) 등에서 다소 높은 사용률을 보였다. 6. 서비스군(54.9%)은 비서비스군(45.1%)에 비해 정기검진을 더 많이 받고 있었으며, 정기검진의 기간에 있어서 1년마다 시행하는 경우가(43.5%) 가장 많았다. 조사결과 서비스 종사자들은 비서비스종사자들에 비해 구강위생활동을 더 활발히 하고 있는 것으로 나타났으며, 관심 또한 많은 것으로 조사되었다. 그러나 일부 구강위생보조용품만을 사용하고 그 외의 구강위생보조용품의 사용은 매우 저조한 것으로 나타났으며, 구강위생보조용품을 사용함에 불편감을 갖고 있었다. 이에 따라 향후 구강위생보조용품의 지속적인 적극적인 홍보가 매우 필요하고, 다양한 교육매체를 이용하여 구강상태에 따라 다양한 구강위생보조용품을 사용할 수 있도록 전문가 교육 및 프로그램 개발이 시급하다. 아울러 보다 편리하게 사용할 수 있는 구강위생보조용품을 개발하고 손쉽게 접근할 수 있도록 판매처를 증가시키고 보급하는 것 또한 필요하다고 사료된다. 본 연구는 몇 가지 점에서 한계를 가지고 있었다. 첫째, 서비스 종사자들 일부만을 대상으로 하여 서비스 종사자들을 모두 대표하기 어렵다는 점이다. 둘째, 구강위생보조용품 사용률은 평가하였으나 사용능력은 평가하지 못했다는 점이다. 즉, 구강위생용품을 정확하게 사용할 수 있는 능력 또한 사용률 못지않게 중요하나 이를 조사하지 못했다. 셋째, 인지도에 따른 사용현황은 조사하였으나 구강건강 상태와 관련하여 사용실태를 파악하지 못했다는 점이다. 따라서 향후 보다 더 실용적인 자료가 되기 위해, 앞선 한계를 보완한 조사도구가 개발되어 보다 광범위한 연구가 필요하다고 사료된다.

  • PDF

일부 중·장년층의 구강위생용품 인지와 사용실태 (A survey about the use of oral hygiene device and awareness some of the middle age people)

  • 정기옥
    • 한국치위생학회지
    • /
    • 제9권4호
    • /
    • pp.580-592
    • /
    • 2009
  • Objectives : This study had been performed for Respondents who live in Daegu and Kyungbuk province Age group of 30 to 50 years old. Methods : The oral health state and oral heath care, dental treatments about the use of oral hygiene devices were obtained through self-administering questionnaires from 2 to 31, January, 2009. Results : 1. The toothpick was well known for respondents and automatic brush, gargle, dental floss were practically used. 2. 57.1% of man know oral hygiene device, they know more about oral hygiene device if they have higher degree, and they use more if they are older than others. 3. 55.5% of respondents who think their oral health condition is not healthy enough recognize oral hygiene device, 77.5% of respondents who visited dental clinic around six month recognize oral hygiene device. 82.4% of respondents who had been follow-up. 86.1% of respondents who think their oral health state is good enough doesn't use oral hygiene device, oral hygiene device was used more for respondents who visited dental clinic frequently. 47.1% of respondents who visited dental clinic periodically use oral hygiene device. 4. Respondents who experience implant recognize more about oral hygiene device and periodontal Tx, orthodontics problem, preservative treatment were next. 5. 60.0% of respondents who were educated tooth brushing method recognize it. Oral hygiene device was frequently used if tooth brushing time were increased. 26.4% of respondents who were changed their tooth brushing method used oral hygiene device. Conclusions : Their recognition level was practically low whose age is around 30 to 50. Their oral hygiene device use ratio is higher then others who were educated tooth brush method so that I think we need to recommend for use oral hygiene device and use method.

  • PDF

The Effects of Biofilm Care on Subgingival Bacterial Motility and Halitosis

  • Kim, Yu-Rin
    • 치위생과학회지
    • /
    • 제19권3호
    • /
    • pp.162-169
    • /
    • 2019
  • Background: Oral diseases are caused by various systemic and local factors, the most closely related being the biofilm. However, the challenges involved in removing an established biofilm necessitate professional care for its removal. This study aimed to evaluate and compare the effects of professional self and professional biofilm care in healthy patients to prevent the development of periodontal diseases. Methods: Thirty-seven patients who visited the dental clinic between September 2018 and February 2019 were included in this study. Self-biofilm care was performed by routine tooth brushing and professional biofilm care was provided using the toothpick method (TPM) or the oral prophylaxis (OP) method using a rubber cup. Subgingival bacterial motility and halitosis (levels of hydrogen sulfide, $H_2S$; methyl mercaptan, $CH_3SH$; and di-methyl sulfide, $(CH_3)_2S$) were measured before, immediately after, and 5 hours after the preventive treatment in the three groups. Repeated measures analysis of variance test was performed to determine significant differences among the groups. Results: TPM was effective immediately after the prevention treatment, whereas OP was more effective after 5 hours (proximal surfaces, F=16.353, p<0.001; smooth surfaces, F=66.575, p<0.001). The three components responsible for halitosis were effectively reduced by professional biofilm care immediately after the preventive treatment; however, self-biofilm care was more effective after 5 hours ($H_2S$, F=3.564, p=0.011; $CH_3SH$, F=6.657, p<0.001; $(CH_3)_2S$, F=21.135, p<0.001). Conclusion: To prevent oral diseases, it is critical to monitor the biofilm. The dental hygienist should check the oral hygiene status and the ability of the patient to administer oral care. Professional biofilm care should be provided by assessing and treating each surface of the tooth. We hope to strengthen our professional in biofilm care through continuous clinical research.

Efficacy of non-surgical treatment accompanied by professional toothbrushing in the treatment of chronic periodontitis in patients with type 2 diabetes mellitus: a randomized controlled clinical trial

  • Lee, Jae Young;Choi, Yoon Young;Choi, Youngnim;Jin, Bo Hyoung
    • Journal of Periodontal and Implant Science
    • /
    • 제50권2호
    • /
    • pp.83-96
    • /
    • 2020
  • Purpose: The present study aimed to evaluate the clinical benefit of additional toothbrushing accompanying non-surgical periodontal treatment on oral and general health in patients with type 2 diabetes mellitus (T2DM). Methods: We conducted a doubled-blind randomized controlled trial in 60 T2DM patients between June 2013 and June 2014. The patients were randomly assigned to the scaling and root planing (SRP) group; the scaling and root planing with additional toothbrushing (SRPAT) group, in which additional toothbrushing was performed by toothpick methods; or the control group. Microbiological and oral examinations were performed for up to 12 weeks following treatment. Non-surgical treatment was conducted in the experimental groups. The SRP group received scaling and root planing and the SRPAT group received additional toothbrushing with the Watanabe method once a week from the first visit through the fifth visit. The primary outcomes were changes in haemoglobin A1c (or glycated haemoglobin; HbA1c) levels, serum endotoxin levels, and interleukin-1 beta levels. Periodontal health status was measured by periodontal pocket depth, the calculus index, and bleeding on probing (BOP). Results: Both the SRP and SRPAT groups showed improvements in periodontal health and HbA1c, but the SRPAT group showed significantly less BOP than the SRP group. Furthermore, only the SRPAT group showed a statistically significant decrease in serum endotoxin levels. Conclusions: Non-surgical periodontal treatment was effective in improving HbA1c and serum endotoxin levels in T2DM patients. Furthermore, non-surgical treatment with additional tooth brushing had a more favourable effect on gingival bleeding management. Trial RegistrationClinical Research Information Service Identifier: KCT000416.

칫솔질 교육 후 치면세균막 지수와 수행도의 변화 (Variation in the Index of Dental Plaque Removal and Practice Assessment after Instruction on Toothbrushing)

  • 김기은;안은숙;한지형
    • 치위생과학회지
    • /
    • 제15권2호
    • /
    • pp.220-225
    • /
    • 2015
  • 본 연구에서는 국내에서 권장하고 있는 회전법이 실천 용이하고 치면세균막 제거에 효과적인지 다른 칫솔질 방법과 비교해 보고, 집단칫솔질교육 후 시간흐름에 따른 효과추이를 확인하고자 임의 추출한 20대 대학생을 대상으로 O'leary 지수와 PHP 지수를 구하고 칫솔질 수행도를 조사하였다. 분석은 STATA 11.0을 이용하였다. 회전법과 바스법, 와타나베법의 칫솔질 방법에 따라 O'leary 지수와 PHP 지수 및 칫솔질 수행도가 차이가 있는지에 대해 조사하기 위해 1차부터 3차까지 평균을 비교한 결과 차이가 없었다. 칫솔질 교육 전인 1차, 칫솔질 교육 2주 후인 2차, 칫솔질 교육 4주 후인 3차의 O'leary 지수, PHP 지수 및 칫솔질 수행도의 차이를 확인하기 위해 비모수 표본자료에 사용하는 Kruskal-Wallis test를 수행한 결과 O'leary 지수의 경우 칫솔질 교육 전 측정 시 가장 낮은 점수를 기록하였고, 2차시기 측정치가 가장 높게 나타났다(p=0.0001). PHP 지수는 1차시기 가장 높게 나타났고, 2차시기 감소하였다가 3차시기 다시 증가하는 것으로 나타났다(p=0.0001). 칫솔질 수행도 역시 PHP 지수와 유사한 경향을 보였다(p=0.0001). 이상의 결과로 회전법이 모든 사람에게 획일적으로 권장할 만한 방법인지 좀 더 다각적인 고찰이 필요하고, 칫솔질 교육효과는 시간이 지나면서 감소되므로 지속적인 교육이 실행될 수 있도록 제도적 장치가 마련되어야 함을 알 수 있었다.