• The Journal of the Korean dental association
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• v.36 no.10 s.353
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• pp.666-669
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• 1998

• The Journal of the Korean dental association
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• v.47 no.5
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• pp.272-281
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• 2009

• BMB Reports
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• v.37 no.3
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• pp.356-361
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• 2004

To develop a simplified method that can rapidly prepare DNA microarray probes in a massive scale, a lambda phage genomic DNA-fragments library was constructed for the microarray-probes collection. Four methods of DNA band recovery from the first PCR products were tested and compared. The DNA microarray probes were collected by a novel method of nested PCR that was mediated by gel isolation of the first PCR products. This method was named GIN-PCR. The probes that were prepared by this GIN-PCR technique were used as subjects to fabricate a DNA microarray. The results showed that a wooden toothpick was superior to the other 3 methods, since this technique can steadily transfer the DNA bands as the template of the second PCR after the first PCR. A group of probes were successfully collected and DNA microarrays were constructed using these probes. Hybridization results demonstrated that this technique of DNA recovery and probe preparation was rapid, efficient, and effective. We developed a cost-effective and less labor-intensive method for DNA microarray probe preparation by nested PCR that is mediated by wooden toothpick transfer of the DNA bands in the gel after electrophoresis.

• Journal of dental hygiene science
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• v.8 no.4
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• pp.261-266
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• 2008

The purpose of this study was to examine the effects of the toothpick method, one of professional toothbrushing methods, on the prevention of periodontal diseases and the satisfaction level of patients with that. The subjects in this study were 33 patients who included 16 men and 17 women. After the toothpick method was applied to the selected patients from January 9 to February 28, 2008, the collected data were analyzed with SPSS (Statistical Package for the Social Science) 12.0 program. The findings of the study were as follows: First, the patients investigated showed a 0.71 reduction in PI than in the past. The index of bleeding upon probing stood at 5.12, which dropped from 8.09 in the beginning. Second, the patients gave a mean of 4.20 to the professional toothbrushing out of possible five points, which showed that they expressed satisfaction with that. Third, as for the relationship between general characteristics and satisfaction level, the women were more satisfied with that ($4.25{\pm}0.33$). By age group, those who were in their 60s and up found that more satisfactory ($4.31{\pm}0.30$). Fourth, as to changes in oral symptoms by gender, the largest number of the men and women considered their gums to become healthier after the professional toothbrushing was applied. The above-mentioned findings suggested that dental institutions should be equipped with well-educated oral health care personnels who are responsible for the periodontal health of patients, and the development of oral health promotion programs is urgently required as well.

• Journal of Korean Dental Hygiene Science
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• v.1 no.1
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• pp.37-46
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• 2018

This study conducted a 'specialist tooth brushing' method against the severely disabled once every other week. The purpose of this study is to analyze and validate the effectiveness of maintaining healthy periodontal management when consistent oral hygiene lasts with minimum stimuli for 24 - months. The conclusions were as follows. Table 2 compares the difference between group 1 and 2 from the 1^st to 11th management and verifies the actual difference in measurement of each index. Group 1 used a professional tooth brush while Group 2 used a general brush. There were found many significant differences in dental index. As for PHP, Oral Malodor index and gingival bleeding, it was found that one or two times of dental care can make substantial differences in dental health condition. These results show that as the number of toothbrush method has been increased, gingival bleeding and periodontal index are significantly improved, not to mention the improvement of gingivitis and periodontal disease. This is because periodontal tissue is affected by brushing method, brushing time and consistency of dental care. During the 6-month period, 20 minutes of active periodontal care was intensively conducted, and for 18-months the effect of consistent care was verified by 10-minute periodontal care once every other week. As a result of the test, it was found that there was no complete regression in the basic periodontal treatment and the periodontal health condition had been maintained for 24 months. Also this test shows that, despite of inconsistent dental hygienic care, regular plague control can prevent dental diseases and maintain the dental health. This study proved that periodontal condition can be maintained by periodontal care once every other week as the tooth brushing properly stimulates the gums with positive effect. Therefore, professional dental healthcareworkers should be designated for each facility for the disabled, and dental health of the disabled should be professionally cared on a regular basis, and consistent and repetitive management by the dental care specialist are required.

• Archives of Plastic Surgery
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• v.44 no.2
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• pp.170-172
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• 2017

There exist some restrictions and difficulties in performing follicular unit extraction (FUE) in white-haired patients, for several reasons. In this paper, we introduce a novel technique for visualizing white hair during the punching procedure and graft preparation in FUE for white-haired patients. In white-haired older male patients, we dyed the surrounding scalp skin purple with a gentian violet solution-stained toothpick. Our method has several advantages: surgeons can easily focus on the center of the follicular unit and rapidly perform punching, they can recognize the condition of the harvested follicular units during FUE, and the hair transplant team can secure a clear view for trimming and loading into the implanter. We suggest that scalp dyeing in difficult FUE procedures, especially in patients with white hair, may be a simple method that provides a good visualization for donor site harvesting and for microdissection.

• Korean Journal of Veterinary Pathology
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• v.5 no.1
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• pp.17-20
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• 2001

We examined the stomaches of slaughtered pigs to evaluate the gastric lesions. Gastric ulcer accompanying parakeratosis and hyperkeratosis were most frequently observed in the mucosa layer of pars oesophagea. Diffuse congestion and focal necrosis was also frequently seen on the fundus. Pierce wounds by toothpick were seen from 3 stomaches.

• Journal of Korean society of Dental Hygiene
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• v.8 no.3
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• pp.37-52
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• 2008

The purpose of this study was to provide basic data for oral health promotion of service worker and their effective interpersonal relationships forming through the evaluation about service workers' awareness of the auxiliary oral hygiene devices and using behavior of it. The survey was carried out questionnaire research targeting 400(service group 200, non-service group 200) and compared the two groups. The obtained results were as follow: 1. brushing behavior showed the highest ratio of 3~4 times/day(53.4%) in brushing frequency, up-down method(50.8%) in brushing method, after 5 minutes after the meals(45.0%) in brushing time, shape brush(56.6%) in brush change time. 2. service group used a lot more auxiliary oral hygiene devices than non-service group(50.9%). the frequency of use was toothpick(30.9%), gargle(29.9%), floss(13.5%), tongue cleaner(10.1%). 3. In comparison of two group about state of use, service group showed more higher ratio of floss(66.1%), tongue cleaner(64.4%), gargle(56.6%) than non-service group. non-service group showed more higher ratio of toothpick(54.6%) than service group 4. Service group used auxiliary oral hygiene devices by suggestions of dental clinic(53.6%) and didn't use them because of uncomfortable to use(45.4%) or didn't know how to use(21.6%). 5. As result of the awareness-related using rate of auxiliary oral hygiene devices, service group showed higher using ratio except toothpick than non-service group. especially gargle(54.8%), tongue cleaner(43.3%), floss(35.8%) were showed high. 6. service group took a regular checkups more than non-service group and showed the highest ratio of each 1 year(43.5%) in checkup period.

• Journal of dental hygiene science
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• v.2 no.2
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• pp.95-103
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• 2002

This study was carried out to investigate recognition level and practical application status of oral hygiene devices through making a survey of Seoul inhabitants. And survey was implemented in order to propose oral hygiene device for household health care activities. The following results were obtained by analyzing personal interviews of 347 commuting patients at two university hospitals and seven dental clinics. 1) The average length of toothbrush head was estimated as 22.3 mm and average changing cycle was 2.3 months. The user ratio of flat-headed brush was estimated as 51.9% and 46.7% were using fluoridated toothpaste. 2) Generally the ratio of toothpick users was higher than other device users. But the user ratio of dental floss was higher than toothpick in case of patients under orthodontic treatment. 3) The patients under orthodontic treatment were not familiar with handling orthodontic toothbrush. Though 45.8% among orthodontic patients recognized this type of toothbrush, only 25.4% of them knew how exactly to use it. 4) It was shown very low user ratio of oral hygiene devices that the patients who had periodontal problem, hypersensitive trouble, halitosis, implant or denture 5) The patients who had halitosis showed the highest user ratio of toothpick. The patients who had separation of teeth showed the highest user ratio of dental floss. The patients who're under orthodontic treatment showed the highest user ratio of interproximal brush and motorized brush. 6) In response to the most interesting dental disease, it's surveyed as follows; 60.3% of dental caries, 24.0% of periodontal disease, 14.8% of false occlusion and 0.9% of oral cancer. 7) Regarding the motivation of using those oral hygiene devices, 45% responded that it was because of recommendation by dental clinics. Among the negative answers, 38.6% responded that it was because of no selection guidance. 31.3% answered that they didn't use hygiene device because it's inconvenient. 12.0% answered that it's difficult to buy and expensive. 7.8% responded that they didn't feel significant improvement. 4.8% answered that dental hospitals and clinics didn't even introduce those hygiene devices. Therefore efficient campaign for those hygiene devices over all Korea nation should be developed and education program must be prepared for each case of patients in every dental hospitals and clinics.

• Journal of Korean society of Dental Hygiene
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• v.9 no.4
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• pp.685-698
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• 2009

Objectives : This study was to examine the recognition and understanding of the use, application of oral hygiene supplies among dental clinic patients in a bid to stress the necessity of education on the right awareness and use of oral hygiene supplies, to provide information on the development of educational programs and ultimately to help promote the oral health of people in general. Methods : The subjects in this study were 314 patients who visited dental clinics in North Jeolla Province in June 2009. Results : 1. Out of oral hygiene supplies, the largest number of the patients investigated(74.6%) were best cognizant of dental floss, and the greatest group(77.7%) had the right understanding of the use of toothpick. Currently, the oral hygiene supplies that were most widely in use were toothpicks(43.2%). 2. As to the relationship of awareness and understanding of the use, application of oral hygiene supplies to subjective oral health status, 50.0 percent of the patients who understood the use of toothpick found themselves to be in good health, and the gaps between them and the others were statistically significant. Among those who were aware of dental floss, the largest group(51.4%) considered themselves to be in good shape, and in the event of those who understood the use of dental floss, the greatest group(49.2%) deemed themselves to be in good health. Out of those who understood the use of mouse rinse, the largest group(53.7%) thought they were in good health(p<0.05). 3. Recognition of interdental brush, understanding of its use and whether to use it currently or not were identified as parents to use oral hygiene supplies recommended by dental clinics(p<0.05). 4. Recognition of interdental brush, understanding of its use and whether to use it currently or not were identified as patients to use oral hygiene supplies recommended by dental clinics(p<0.05). 5. The largest group of those who didn't put dental floss to use didn't use it for other reasons unspecified in the questionnaire, and the second greatest group of them didn't use it since it was so onerous to do that. There were statistically significant differences in the reason why they didn't use the oral hygiene supplies(p<0.05). 6. Awareness of dental floss and interdental brush, understanding of the use of the two and whether to use the two at present or not made statistically significant differences to whether they were likely to use the oral hygiene supplies in the future. And whether they were likely to use the oral hygiene supplies in the future was statistically significantly different according to awareness of mouse rinse and understanding of the use of it as well(p<0.05). Conclusions : Dental hygienists have to provide patients with various data of oral hygiene devices through oral health education and then only patients caring in dental clinics can choose the appropriate devices to claim for their own disease.