• Genomics & Informatics
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• v.14 no.1
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• pp.29-33
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• 2016

A retron is a bacterial retroelement that encodes an RNA gene and a reverse transcriptase (RT). The former, once transcribed, works as a template primer for reverse transcription by the latter. The resulting DNA is covalently linked to the upstream part of the RNA; this chimera is called multicopy single-stranded DNA (msDNA), which is extrachromosomal DNA found in many bacterial species. Based on the conserved features in the eight known msDNA sequences, we developed a detection method and applied it to scan National Center for Biotechnology Information (NCBI) RefSeq bacterial genome sequences. Among 16,844 bacterial sequences possessing a retron-type RT domain, we identified 48 unique types of msDNA. Currently, the biological role of msDNA is not well understood. Our work will be a useful tool in studying the distribution, evolution, and physiological role of msDNA.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2004.10a
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• pp.60-66
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• 2004

Hardness value decreased about 3% for annealed specimens and increased about 60% for one hour aged specimens. But the values of the other specimens aged two hours or more showed almost the same. The yield strength was the highest about 1,800㎫ in 0.06%Nb specimen having twice as much as the base meta1 specimen. Also, the elongation was the highest in 0.03%Nb specimen showing the same as base metal specimen. The higher aging temperature and the longer aging time, the higher fatigue life. On the other hand, the 0.03%Nb specimen showed the highest fatigue life which increased about 12% more than base metal specime. 0.06%Nb specimen aged at 482$^{\circ}C$ for 8 hours simultaneously satisfied the 250 grade strength and 200 grade elongation having the most superior mechanical properties.

• Molecules and Cells
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• v.23 no.2
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• pp.123-131
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• 2007

Extracellular stresses induce heat shock response and render cells resistant to lethal stresses. Heat shock response involves induction of heat shock proteins (Hsps). Recently the roles of Hsps in neurodegenerative diseases and cancer are attracting increasing attention and have accelerated the study of heat shock response mechanism. This review focuses on the stress sensing steps, molecules involved in Hsps production, diseases related to Hsp malfunctions, and the potential of proteomics as a tool for understanding the complex signaling pathways relevant to these events.

• Proceedings of the KSR Conference
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• 2008.06a
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• pp.1128-1130
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• 2008

Bimodal tram is a new public transportation to improve the existing traffic system. The purpose of this study was to investigate the environmental characteristics of the developed bimodal tram through its life cycle. The used tool was life cycle assessment (LCA), which could evaluate environmental loads quantitatively for a product. Based on bill of material (BOM), the environmental impacts of bimodal tram was calculated from manufacturing to operating phase. Among impact categories, photochemical oxidant creation potential (POCP) was the highest, which was resulted from the manufacturing and the combustion of CNG used as a fuel of bimodal tram. In the future, the application of LCA results can enhance increasingly the environment of bimodal tram as an environmental-friendly transportation.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.10 no.6
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• pp.109-115
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• 2001

This paper investigates the mechanical characteristics of angle-ply laminates with non-woven carbon tissue. The lami- nates were made by inserting non-woven carbon tissue at the interface. Specimens were rounded near the tabs by grinding and polishing to reduce the stress concentration. Cyclic loads were applied to the specimens and the stress and fatigue life curves were obtained. The matrix crack density was also evaluated to check the effects of non-woven carbon tissue on the fracture resistance of composite laminates. C-Sean technique was used to evaluate the delamination, and SEM was used to understand the fracture mechanisms of the laminates. Experimental results show that the fatigue strength and life of composite laminates were increased by inserting non- woven carbon tissues. The results also show that the matrix crack density and delamination area were reduced by inserting non-woven carbon tissues.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.14 no.6
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• pp.83-87
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• 2005

Hardening Constant Velocity joint increases hardness near the surface and the surface improves fatigue life. Although case depth and chemical composition are same, the prior structure of induction hardened Constant Velocity Joint affects the fatigue strength and life during hardening. Therefore torsional fatigue tests of specimens from vaere conducted on induction hardened automotive Constant Velocity joint with various case depths and lrious prior structures, which are obtained by nomalizing, spheriodized annealing and tempering after quenching, woads applied in order to evaluate the relation between prior structure and fatigue strength.

• Journal of Surface Science and Engineering
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• v.34 no.4
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• pp.337-341
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• 2001

TiN coating of high speed end mill is recently generalized. The study of coating layer using ion plating is mainly about the coating method and the why of the longer life of coated tools. In CNC machning process, metal cutting isn't carry out until the tools including the end-mill and so on are fractured. Namely, it is difficult precision processing when the cutting force of the cutting tool is near the limit the fracture cutting force. So, the estimate of the life by wear and fracture is important. Therefore, this study is about the method to estimate the capacity of the coating layer in relation to the tendency of cutting force and the influence of the cutting capacity of coated end-mill by the condition N2, Ar, temperature. The cutting length is in inverse proportion to the cutting force ratio. So, the life of the TiN coated end mill can be predicated by the ratio of the increase of the cutting force.

• Journal of Radiation Industry
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• v.4 no.3
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• pp.215-219
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• 2010

We have subjected to gamma-irradiation to citrus buds and then grafted onto mature citrus tree. Mutant citrus branch lines have been induced. As a result of first selection, we found the several mutant lines showing interesting phenotypes such as higher sugar content. We have selected several branches showing good qualities such as higher sweetness and/or lower acidity. Some branch lines showed over $13^{\circ}Brix$ sugar content and below 0.9% acidity. Other mutant branch lines showed the changes of shape, size, peel thickness, and fiber contents or distribution of fruits. The results suggest that gamma-irradiation is an effective tool for induction of citrus mutant lines.

• Genomics & Informatics
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• v.16 no.4
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• pp.30.1-30.6
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• 2018

There is urgent need for effective and cost-efficient data storage, as the worldwide requirement for data storage is rapidly growing. DNA has introduced a new tool for storing digital information. Recent studies have successfully stored digital information, such as text and gif animation. Previous studies tackled technical hurdles due to errors from DNA synthesis and sequencing. Studies also have focused on a strategy that makes use of 100-150-bp read sizes in both synthesis and sequencing. In this paper, we a suggest novel data encoding/decoding scheme that makes use of long-read DNA (~1,000 bp). This enables accurate recovery of stored digital information with a smaller number of reads than the previous approach. Also, this approach reduces sequencing time.

• IMMUNE NETWORK
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• v.16 no.1
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• pp.33-43
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• 2016

Cell-penetrating peptides (CPPs) are short amino acids that have been widely used to deliver macromolecules such as proteins, peptides, DNA, or RNA, to control cellular behavior for therapeutic purposes. CPPs have been used to treat immunological diseases through the delivery of immune modulatory molecules in vivo. Their intracellular delivery efficiency is highly synergistic with the cellular characteristics of the dendritic cells (DCs), which actively uptake foreign antigens. DC-based vaccines are primarily generated by pulsing DCs ex vivo with various immunomodulatory antigens. CPP conjugation to antigens would increase DC uptake as well as antigen processing and presentation on both MHC class II and MHC class I molecules, leading to antigen specific CD4⁺ and CD8⁺ T cell responses. CPP-antigen based DC vaccination is considered a promising tool for cancer immunotherapy due to the enhanced CTL response. In this review, we discuss the various applications of CPPs in immune modulation and DC vaccination, and highlight the advantages and limitations of the current CPP-based DC vaccination.