• The Plant Pathology Journal
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• v.39 no.5
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• pp.494-503
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• 2023

Xanthomonas campestris pv. campestris (Xcc) is a plant pathogen of Brassica crops that causes black rot disease throughout the world. At present, 11 physiological races of Xcc (races 1-11) have been reported. The conventional method of using differential cultivars for Xcc race detection is not accurate and it is laborious and time-consuming. Therefore, the development of specific molecular markers has been used as a substitute tool because it offers an accurate and reliable result, particularly a quick diagnosis of Xcc races. Previously, our laboratory has successfully developed race-specific molecular markers for Xcc races 1-6. In this study, specific molecular markers to identify Xcc race 7 have been developed. In the course of study, whole genome sequences of several Xcc races, X. campestris pv. incanae, X. campestris pv. raphani, and X. campestris pv. vesicatoria were aligned to identify variable regions like sequence-characterized amplified regions and insertions and deletions specific to race 7. Primer pairs were designed targeting these regions and validated against 22 samples. The polymerase chain reaction analysis revealed that three primer pairs specifically amplified the DNA fragment corresponding to race 7. The obtained finding clearly demonstrates the efficiency of the newly developed markers in accurately detecting Xcc race 7 among the other races. These results indicated that the newly developed marker can successfully and rapidly detect Xcc race 7 from other races. This study represents the first report on the successful development of specific molecular markers for Xcc race 7.

• Animal Bioscience
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• v.36 no.6
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• pp.973-979
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• 2023

Objective: The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, which is the most efficient and reliable tool for precisely targeted modification of the genome of living cells, has generated considerable excitement for industrial applications as well as scientific research. In this study, we developed a gene-editing and detection system for chick embryo sexing during the embryonic stage. Methods: By combining the CRISPR/Cas9 technical platform and germ cell-mediated germline transmission, we not only generated Z chromosome-targeted knockin chickens but also developed a detection system for fluorescence-positive male chicks in the embryonic stage. Results: We targeted a green fluorescent protein (GFP) transgene into a specific locus on the Z chromosome of chicken primordial germ cells (PGCs), resulting in the production of Z^GFP-knockin chickens. By mating Z^GFP-knockin females (Z^GFP/W) with wild males (Z/Z) and using a GFP detection system, we could identify chick sex, as the GFP transgene was expressed on the Z chromosome only in male offspring (Z^GFP/Z) even before hatching. Conclusion: Our results demonstrate that the CRISPR/Cas9 technical platform with chicken PGCs facilitates the production of specific genome-edited chickens for basic research as well as practical applications.

• 한국정보교육학회:학술대회논문집
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• 2021.08a
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• pp.303-308
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• 2021

In this study, we intended to present a class design plan that combines safety education and software education, which are very important in the school field, but are considered necessary for change. In order to realize safety education centered on indirect experience, the 12th class plan was devised as a "Smart Safety Commuter Road Production Project" using a physical computing tool called Bitbrick. Based on the 12th city's class plan, qualitative research was conducted with interviews with a total of five teachers, showing both the effectiveness and problems of applying them to the class. The effectiveness is that unlike conventional delivery-type safety education, indirect experience-oriented safety education centered on real-life situations is possible, and it can be expected that procedural thinking skills can be developed along with stimulating interest. However, the problem has been shown that the process of implementing the direct situation can be challenging and that even activities can be more efficient than group activities.

• International Journal of Advanced Culture Technology
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• v.11 no.2
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• pp.152-161
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• 2023

Modern people live a life connected with the real world and the virtual world by relying on the new media of enterprises and social consumption led by innovative technologies. In this environment, virtual influencers actively communicate with consumers and build relationships through social media, which is a new marketing tool that has attracted widespread attention. From a business perspective, it is necessary to have a solid understanding of this phenomenon, and then explore communication strategies to effectively develop virtual influencers. To investigate followers' preference for virtual influencers, this study employs the Q-method, which studies human subjective attributes, an empirical research effort to uncover complex issues in human subjectivity. To determine the factors that trigger people's voluntary and active practice and the preference degree of virtual influencers, the Q method is implemented to examine human subjectivity, thoughts and attitudes. According to the results of this study, virtual influencers are a new group of idols full of vitality. The interviews found that there are still many virtual influencers who do not know about followers, but each type can be clearly understood through the intuitive understanding of the interviewees. Divided out, type 1 one egoideal virtual influencers aim to represent an idealized version of the creator or target audience. Embodies ideal physical characteristics, personality or lifestyle desired by the audience. Type 2 is charismatic and attractive, and has the characteristics of most virtual influencers. It is suggested that it can be developed into a potential type, doing brand cooperation, and content production on social media platforms. Type 3: Game animation, derived from the image of characters in games or comics, with stylized features and energetic personalities, which can be integrated into games or entertainment experiences. Type 4 development potential type is the most successful type among virtual imagers, and it is also the purpose of marketing virtual influencers. It is essential that brand endorsement on social media platforms, integrated marketing, and driving advertising traffic. It is recommended to improve production technology to reduce investment costs.

• The Journal of Internal Korean Medicine
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• v.44 no.3
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• pp.285-293
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• 2023

Background: Irritable bowel syndrome (IBS) is a digestive disorder characterized by abdominal discomfort or pain accompanied by a change in stool condition. Owing to its complicated mechanisms, a standard treatment for IBS has not yet been established. Yukgunja-tang (YGT) is a Korean herbal medicine known in Asia to be effective in the treatment of gastrointestinal symptoms. In this study, we will conduct a systematic review of randomized controlled trials (RCTs) to assess the efficacy and safety of YGT in IBS treatment. Methods and analysis: English databases, such as Embase, Medline (via PubMed), Allied and Complementary Medicine Database, and Cochrane Central Register of Controlled Trials, will be searched for articles published up to April 2023. Additional databases, such as five Korean, one Chinese, and one Japanese database, will be included. RCTs and quasi-RCTs will also be included in the assessment of the efficacy of YGT. The overall efficacy rate will be the primary outcome, and data such as IBS quality-of-life measurements, global symptom scores, and adverse events will be the secondary outcomes. Review Manager Version 5.3 will be used for evaluation, and the risk of bias (RoB) will be evaluated using Cochrane Collaboration's RoB tool. The Grading of Recommendations Assessment, Development, and Evaluation approach will be used to score the quality of evidence. Conclusion: This study will demonstrate the efficacy and safety of YGT for treating patients with IBS.

• Convergence Security Journal
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• v.22 no.2
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• pp.43-50
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• 2022

It is no exaggeration to say that mobile devices have already become an essential tool in our daily life. Among these mobile devices, a representative smart phone is overheating the market by introducing new functions and services whenever a new product is released. However, most users do not know that there are various vulnerabilities depending on the manufacturer, service, or function, and damage is occurring due to attacks that exploit the vulnerabilities.Research on this has already been conducted, but it is very difficult to predict because there are various differences depending on new devices, operating systems, services, and functions. For this reason, it is necessary to continuously monitor and study new vulnerable factors. Therefore, through this study, research so far, vulnerabilities, attack technology, and response technology were considered. In addition, it is expected that it can be used as basic data for the development of systems and response technologies in the future by proposing countermeasures.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.1B
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• pp.21-32
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• 2008

Flood forecasting is a very important tool as one of nonstructural measures for reduction of flood damages in life and property and its accuracy is also an important factor. However, when we apply the Storage Function Model(SFM) which is mainly used for the flood forecasting system in Korea, the determination of the parameters is very important but it is difficult. So, the parameters have been calibrated by using an empirical formulas and judgement of hydrologist. Hence, in this study we perform the sensitivity analysis to understand the parameter characteristics and establish the ranges of parameters of the SFM. Also we do the parameter calibration by using the optimization techniques and objective functions, and evaluate their performances. Especially, we suggest a method to determine proper parameters by using a objective function which can be obtained from flood events. So, we use the suggested method for parameter estimation and compare the estimated parameters with the previously reported parameters. As a result of the application, the estimated parameters by the suggested method showed better than them from the previously reported parameters.

• Journal of English Language & Literature
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• v.58 no.1
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• pp.163-183
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• 2012

This paper is one of those attempts to explore some possibility of agreement between feminist discourse and postcolonial discourses through the approach of postcolonial feminism in the reading of the controversial novel, So Far from the Bamboo Grove and Year of Impossible Goodbyes. So Far from the Bamboo Grove, when read from the perspective of postcolonial feminism, reveals 'domestic nationalism' of imperial narratives in which the violence of imperial history in Korea is hidden behind the picture of every day lives of an ordinary Japanese family and Japanese women. Furthermore, postcolonial feminist's perspective interprets Yoko family's nostalgia for their 'home,' Nanam in Korea, as 'imperialist nostalgia' working as a mask to hide the violent history of colonization of Empire. In this way, postcolonial feminist reading of the story detects the ways the narrative of Empire appropriates women, family image and even nostalgia for childhood. At the same time, this perspective explains the readers' empathy for Yoko family's suffering and the concerning women issues caused by wartime rape and sexual violence by defining Yoko as a woman of Japanese Empire, whose life of interstice between imperial men and colonial men cannot be free from violence of rape during anti colonial wars. Year of Impossible Goodbyes as a counter discourse does not overcome the traditional binary opposition of nationalism which quietens gender and class issues. As an attempt to fill in the interstice between the two perspectives of feminism and postcolonialism. postcolonial feminist reading turns out to be a valid tool for the reading of the two novels chosen here.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2023.05a
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• pp.132-133
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• 2023

The safe evacuation of people during disasters is of utmost importance. Various life safety evacuation simulation tools have been developed and implemented, with most relying on algorithms that analyze maps to extract the shortest path and guide agents along predetermined routes. While effective in predicting evacuation routes in stable disaster conditions and short timeframes, this approach falls short in dynamic situations where disaster scenarios constantly change. Existing algorithms struggle to respond to such scenarios, prompting the need for a more adaptive evacuation route algorithm that can respond to changing disasters. Artificial intelligence technology based on reinforcement learning holds the potential to develop such an algorithm. As a fundamental step in algorithm development, this study aims to evaluate whether an evacuation algorithm developed by reinforcement learning satisfies the performance conditions of the evacuation simulation tool required by IMO MSC.1/Circ1533.

• Journal of Digestive Cancer Research
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• v.1 no.1
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• pp.6-16
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• 2013

Recent estimates for colon cancer incidence in Korea have been increased and continue to rank as the second most common in male and the third in female. Although colonoscopy has been known as the best screening tool for colon cancer, 20-25% of patients with colon cancer was diagnosed with stage IV cancer. During the past 10 years, intensive clinical studies helped to establish the value of palliative treatment for colon cancer with metastasis. The introduction of new chemotherapeutic agents such as irinotecan and oxaliplatin has led to a significant increase in tumor response and median survival. In advanced colon cancer, impressive prolongation or overall survival can be achieved through sequential application of combined systemic chemotherapy. In addition, targeted manipulation of molecular tumor mechanisms with new substances such as monoclonal antibodies against the epidermal growth factor receptor or vascular endothelial growth factor shows promising effects. Progress in the systemic treatment of colon cancer is evident, not only because of the significant increase in life expectancy in advanced colon cancer.