To determine the critical level for optimum maturity of flue-cured tobacco leaves (KF118) at the stalk position from cutter to tips, the reflectance index using ground-based remote sensors and chlorophyll meter were investigated. The sensors estimated were Crop $Circle^{TM}$ (Holland Scientific), Green $Seeker^{TM}$ (Ntech Industries), Spectroradiometer (LICOR, LI-1800), Chlorophyll meter (SPAD502, Minolta), and Field $Scout^{TM}$ Chlorophyll meter (CM-1000, Spectrum). The L, a, b values and greenness for flue-cured leaf were measured and estimated for correlation with sensor's measurement of harvested leaf. On a reflectance curve of 340nm~1100 nm, the reflectance peaks on 550nm and 675 nm for the harvested leaf were lowered as change from light green to darker green. Darker green leaf harvested produced darker flue-cured leaf. The reflectance at 675 nm for flue-cured leaf decreased as greenness increased in the harvested leaf. This result means that the red edge band of 675 nm wavelength is related to the absorbance of chlorophyll for photosynthesis. The greenness of flue-cured leaf showed significantly positive correlation with the entire reflectance indexes for harvested leaf while the L value by colorimeter showed negative correlation with greenness of cured leaf. The critical level for optimum maturity of harvested leaf were less than 22, 135, and 0.43 for SPAD reading, CM-1000 reading, and gNDVI by Crop $Circle^{TM}$, respectively. Consequently, ground-based remote sensing providing a non-destructive real-time assessment of plant greenness could be a useful tool in the selection of optimum maturity of flue-cured tobacco leaves in relation to high quality of flue-cured tobacco.
Journal of The Korean Association For Science Education
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v.34
no.7
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pp.657-666
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2014
To identify the gifted, it is essential to perform overall evaluation on cognitive and affective aspects considering all the characteristics of the science gifted. Nowadays, not only cognitive factors but also affective factors are being emphasized. Among the affective factors of the gifted, the task commitment is an important factor to describe the gifted and their outstanding achievements. From this research, by measuring the characteristics of task commitment shown in the science gifted, this can offer good implications regarding the selection of the gifted and the education. We developed the rubric of the gifted students by analyzing the students' experience of showing task commitment. By applying the rubric, we measured the levels by areas of the characteristics of task commitment shown in the experiences which the science gifted had by deeply exploring the cause or the principle. To better understand the characteristics of the science gifted students' task commitment, each and every students' characteristics were specifically described. The students' task commitment can be measured objectively and effectively by using the measuring tool in the form of rubric based on the characteristics of the task commitment. Specifically describing the students' characteristics on the basis of their performance criteria is the grounds for the level judgment and enhances the understanding of the characteristics of students' task commitment.
Triploids are a useful tool for biomass production and molecular breeding of trees with a long life span. Triploids of the poplar 'Hyunsasi' (Populus alba ${\times}$ P. glandulosa) have been developed by crossing between female diploids and a male tetraploid. The tetraploid was developed around the 1970s at Korea Forest Research Institute by colchicine-induced chromosome doubling. Seedlings of the $F_1$ generation were analyzed using flow cytometry to verify their ploidy status. The mean relative fluorescence index of 3 F1 poplars, labeled as Line- 1, Line-17, Line-18, were approximately 1.5 times higher than those of diploid poplars, and the results clearly indicated that they were triploids. The phenotype of the F1 poplars included larger leaves and thicker stem than diploids, and abnormal leaf morphology, especially in the triploid 'Line-18'. Three triploid lines developed roots more slowly and had less roots than diploid. However, 3 poplar cytotypes (2x, Line-1, Line-17) rooted within 10 days on MS medium. In contrast, compared with the 3 cytotypes, the Line-18 showed about 80% and 70% in the rooting rate and the number of roots. The triploid poplars could be directly utilized for biomass production and with their sterility, they could serve as basic material for genetic transformation. In addition, flow cytometric analysis proved to be an effective and reliable method for screening forest trees for their ploidy level.
Cho Hwa-Young;Park Jeong-Jin;Yoon Sung-Yong;Part Jong Moon
KSBB Journal
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v.20
no.4
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pp.285-290
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2005
Benzophenanthridine alkaloids - sanguinarine, chelirubine, macarpine, and chelerythrine are produced from Eschscholtzia californica (Californica Poppy, used as a sedative by Native Americans) and most of them are derived from dihydrosanguinarine. The properties of sanguinarine are the basis of its antimicrobial activity and its use in chemosurgery and skin cancer excision. For overproduction of sanguinarine from E. californica, yeast extract was used as elicitor and the elicited cell's metabolites were checked. Sanguinarine production was increased intracelluarly about 8 times in the cell and 5 times extracelluarly. We have peformed proteomic analysis of proteins sequentially extracted from E. califormica suspended cells which were cultured with elicitor, an increase of spot intensity was seen at 24 hours following elicitation. These proteins were separated by two-dimensional electrophoresis (2-DE). We found several spots that were expected to be related to benzophenanthridine alkaloids production by comparing the production profiles of metabolites such as sanguinarine. These results demonstrate the use of metabolite analysis as a tool for detecting target proteins related to metabolites production pathway.
Lee, Joo-Hyun;Park, Jin-Hyuck;Kim, Yeonju;Park, Hae Yean;Park, Ji-Hyuk
Therapeutic Science for Rehabilitation
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v.5
no.2
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pp.57-69
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2016
Objective: The purpose of this study was to systematically review the articles using accelerometer to measure activity of daily living of patients with stroke. Methods: Depending on selection criteria, 13 studies were searched for PubMed, EMBASE, and Cochrane library database from February 2014 to March 2014. A total of 331 papers were searched, and 13 of these were selected. Results: In studies of 13 selected, acute, subacute, and chronic patients with stroke were enrolled. The kind of accelerometer was uniaxial, biaxial, and triaxial, activity monitor. Measurement activities were mainly arm activity, walking activity, and attachment sites were also various depending on the measurement activities. A measured variable was the total number of activities, the movement speed of the patients, ratio between affected and non-affected, and motion analysis. The result indicated that significant correlation with the other assessment tools in all studies. Conclusions: Accelerometer will be applied with a tool for measuring activity of daily living of patients with stroke, depending on activities characteristics. Further, we need accelerometer studies to apply with a variety of assessment in clinical practice or community settings.
Journal of Korean Academy of Fundamentals of Nursing
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v.8
no.2
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pp.132-146
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2001
Purpose: This study was to analyze the trends and content of all the research (171 research papers) published in the Journal of Fundamentals of Nursing from 1994, when it began to 2000 using an objective tool developed by the researches, and to identify the direction for the future research in Fundamentals of Nursing. Method: Research published in the Journal of Fundamentals of Nursing from 1994 were reviewed. Result: Most of research (94.2%) was non-degree based and funded research was very limited. The average number of researchers per research study was 2.2 and collaborative research has been gradually increasing. The total number of key words was 331 and the average was 2.6 per research. The major key words were not different from other nursing departments and included home care nursing (10 times), health promotion (8 times), self-care (7 times). self-efficacy (7 times), and homo-dialysis (6 times). Eighty seven percent of the research was quantitative research. and there were very few qualitative studies. Considering theory level, it was found that 40% were factor related research For study design, non-experimental studies were most frequent (66%), and the rate, especially of surveys, has remarkably decreased. Selection of subjects by convenience sampling, was most frequent and there were very few studies that provided the rationale for the calculation of sample size. The major subjects of study were patients (44.8%) with various diagnoses. The subjects usually gave oral consent to take part in that study. Giving information (46.9%) and exercise (26.5%) were common nursing interventions, and physiological indices (16.5%). vital signs (10.3%), physical functioning (8.2%), level of knowledge/skill (7.2%) and level of activity (6.2%) were frequently measured as outcome. Variables questionnaires were the major approach used to collect data, and 57.8% of the research provided the Cronbach alpha to guarantee internal consistency of study instruments. Data were analyzed with computerized statistical packages using, ANOVA (42.0%), T test(39.5%), and chi-square test. For the last seven years, nursing research in Fundamentals of Nursing has gradually improved in both quantify and quality. Conclusion: It was difficult to find any uniqueness or difference compared to other departments of nursing. In fact, because the history of the Journal of Fundamentals of Nursing is rather short, we can expect that there will be further improvement in qualify and content in the future.
Purpose: The utility of 18-fluordesoxyglucose positron emission tomography ([18F]-FDG-PET) combined with computer tomography or magnetic resonance imaging (MRI) in gastric cancer remains controversial and a rationale for patient selection is desired. This study aims to establish a preclinical patient-derived xenograft (PDX) based [18F]-FDG-PET/MRI protocol for gastric cancer and compare different PDX models regarding tumor growth and FDG uptake. Materials and Methods: Female BALB/c nu/nu mice were implanted orthotopically and subcutaneously with gastric cancer PDX. [18F]-FDG-PET/MRI scanning protocol evaluation included different tumor sizes, FDG doses, scanning intervals, and organ-specific uptake. FDG avidity of similar PDX cases were compared between ortho- and heterotopic tumor implantation methods. Microscopic and immunohistochemical investigations were performed to confirm tumor growth and correlate the glycolysis markers glucose transporter 1 (GLUT1) and hexokinase 2 (HK2) with FDG uptake. Results: Organ-specific uptake analysis showed specific FDG avidity of the tumor tissue. Standard scanning protocol was determined to include 150 μCi FDG injection dose and scanning after one hour. Comparison of heterotopic and orthotopic implanted mice revealed a long growth interval for orthotopic models with a high uptake in similar PDX tissues. The H-score of GLUT1 and HK2 expression in tumor cells correlated with the measured maximal standardized uptake value values (GLUT1: Pearson r=0.743, P=0.009; HK2: Pearson r=0.605, P=0.049). Conclusions: This preclinical gastric cancer PDX based [18F]-FDG-PET/MRI protocol reveals tumor specific FDG uptake and shows correlation to glucose metabolic proteins. Our findings provide a PET/MRI PDX model that can be applicable for translational gastric cancer research.
Lung cancer, one of the leading causes of cancer-related deaths, usually appears as solitary pulmonary nodules (SPNs) which are hard to diagnose using the naked eye. In this paper, curvelet-based textural features and clinical parameters are used with three prediction models [a multilevel model, a least absolute shrinkage and selection operator (LASSO) regression method, and a support vector machine (SVM)] to improve the diagnosis of benign and malignant SPNs. Dimensionality reduction of the original curvelet-based textural features was achieved using principal component analysis. In addition, non-conditional logistical regression was used to find clinical predictors among demographic parameters and morphological features. The results showed that, combined with 11 clinical predictors, the accuracy rates using 12 principal components were higher than those using the original curvelet-based textural features. To evaluate the models, 10-fold cross validation and back substitution were applied. The results obtained, respectively, were 0.8549 and 0.9221 for the LASSO method, 0.9443 and 0.9831 for SVM, and 0.8722 and 0.9722 for the multilevel model. All in all, it was found that using curvelet-based textural features after dimensionality reduction and using clinical predictors, the highest accuracy rate was achieved with SVM. The method may be used as an auxiliary tool to differentiate between benign and malignant SPNs in CT images.
Journal of Korean Tunnelling and Underground Space Association
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v.9
no.1
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pp.75-82
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2007
As one of the main support systems, rock bolts play a crucial role in the reinforcement of tunnels. Numerical and experimental studies using a transmission method of ultrasonic guided waves are performed to evaluate the integrity of rock bolts encapsulated by grouting paste. Numerical simulations using "DISPERSE" are carried out for the selection of the optimal experimental setup, i.e. non-destructive testing (NDT) system of the rock bolt. Based on results of the numerical simulation, the calculated frequency range for NDT testing is between 20kHz and 70kHz with the first longitudinal L(1) mode. Laboratory transmission tests are performed by attaching the piezo electric sensor at the tip of the rock bolt before embedding. Both of analytical and experimental results show that the amplitude of signals as well as the wave velocity increases with increase in the defect ratio of grouting paste. The defect in grouting paste means that the space around the rock bolt is not fully filled with the grouting paste. Experimental results also show that the increase of the wave velocity is more sensitive to the defect ratio increase than that of the amplitude. This study demonstrates that the transmission technique of ultrasonic guided waves may be a valuable tool in the evaluation of the rock bolt integrity.
Journal of the Korea Academia-Industrial cooperation Society
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v.19
no.6
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pp.176-187
/
2018
The Korean government has been encouraging 'continuous' post-pump priming project management through relevant local personnel planning, action, and management, upon the selection of 68 sites for urban regeneration as part of the New Deal Project. This study assessed the utilization characteristics of containers, the socio-experimental temporary occupation facilities, as a tool for 'sustainable' urban regeneration. This is to identify the potential limitations of urban regeneration by the utilization of containers as public sector temporary occupation facilities and to provide adequate directions based on its efficacy. By comparing three Korean cases with the Japanese WaiWai project, in terms of 10 elements, the preoccupations with the social trend and scenery, and undifferentiated functions of the facilities focusing on the commercial or working aspects have been identified as limitations of Korean cases. Based on the analyses, the following are suggested as plausible approaches to utilize socio-experimental containers for sustainable urban regeneration: 1. Planning and management should be done by the same organization because the absence of the planning party's participation in the management likely leads to the pursuit of only profits. 2. Diversification and combinations of different utilization purposes of a single site and numerous planning of experimental container uses are in need. 3. Accommodations of heavy commercial uses lead to a decline in the continuity of follow-up projects and a change in the urban regeneration directions. Therefore, it is important to include a public socio-experimental program rather than a simple commercial or promotion program for the following urban renewal project.
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