• The Korean Journal of Mycology
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• v.45 no.4
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• pp.259-269
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• 2017

In order to investigate the diversity of yeasts from major rivers (the Daejeoncheon, Gapcheon and Yudeungcheon) located in Daejeon city, we isolated wild yeasts by plating diluents of samples collected during the summer and winter of 2016 onto yeast extract-peptone-dextrose (YPD) medium, then identified them using Basic Local Alignment Search Tool (BLAST) analysis to compare the nucleotide sequences of the PCR amplicons for the D1/D2 domain of 26S rDNA. In total, we isolated 191 yeast strains belonging to 104 species from 148 soil or water samples from the rivers and their junctions. Candida spp. (45 strains) including Candida tropicalis (22 strains) were the most abundantly isolated strains from the Daejeoncheon. Candida spp. (16 strains) including Candida vartiovaarae (8 strains) and Candida spp. (18 strains) such as Candida sake (4 strains) were also the dominant isolates from the Gapcheon and Yudeungcheon, respectively. In conclusion, Candida spp. and Cryptococcus spp. were the most dominant strains, corresponding to 42% and 7% of the 191 yeast strains isolated in this study, respectively.

• Tuberculosis and Respiratory Diseases
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• v.69 no.5
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• pp.331-336
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• 2010

Background: Recently, the rate of infections with non-tuberculous mycobacteria (NTM) has been increasing in Korea. Precise identification of NTM is critical to determination of the pathogen and to target treatment of NTM patients. Methods: Sixty-eight unclassified mycobacteria isolates by rpoB PCR-RFLP assay (PRA) collected in 2008 were analyzed by National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST) search after sequencing of 16S rRNA, hsp65, rpoB genes. Results: Nineteen strains of 68 isolates were specified as species after sequencing analysis of 3 gene types. We found 3 M. lentifulavum, 5 M. arupense, 4 M. triviale, 4 M. parascrofulaceum, and one M. obuense. One M. tuberculosis and another M. peregrinum were mutated at the Msp I recognition site needed for rpoB PRA. The remaining 49 isolates did not coincide with identical species at the 3 kinds genes. Conclusion: Sequencing analysis of 16S rRNA, hsp65, rpoB was useful for identification of NTM unclassified by rpoB PRA.

• Physical Therapy Rehabilitation Science
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• v.5 no.1
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• pp.22-28
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• 2016

Objective: The Back Mapper is one type of Rasterstereography and it can be used in the clinic without radiation exposure. The purpose of our study was to prove the reliability and validity of the Back Mapper and to compare it with the Spinal Mouse, which is an assessment tool for spinal curvatures using a wheeled mouse, and the Cobb angle by X-ray. Design: Cross-sectional study. Methods: Twenty healthy adults participated in the test to investigate for the inter-rater reliability, intra-rater reliability, and concurrent validity. The tests were performed with assessment devices for scoliosis such as the Back Mapper, Spinal Mouse and Cobb's angle. Data was analyzed by an intraclass correlation coefficient (ICC) value and a standard error of measurement for reliability and correlation analysis for validity. Results: Intra-rater reliability of the Back Mapper was good (Cronbach's ${\alpha}$=0.821-0.984, ICC=0.696-0.969) except for assessing the lordotic angle. Inter-rater reliability was good (Cronbach's ${\alpha}$=0.870-0.958, ICC=0.770-0.919) in assessment for trunk imbalance, rotation of scapulae, thoracic angle, lumbar angle, and kyphotic angle. The kyphotic angle in the Spinal Mouse had a significant correlation icompared with the Back Mapper (r=0.510, p<0.05), and the Cobb's angle from an X-ray had a significant correlation with trunk inclination (r=0.532, p<0.05). Conclusions: These findings provide good intra-reliability of the Back Mapper in healthy subjects, but the Back Mapper requires more experienced practice to have good inter-reliability. Also, the variables of the Back Mapper does not seem as appropriate compared with the Cobb angle by X-ray.

• Journal of KIISE:Software and Applications
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• v.35 no.7
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• pp.442-451
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• 2008

Most plagiarism detection systems evaluate the similarity of source codes and detect plagiarized program pairs. If we use the source codes in plagiarism detection, the source code security can be a significant problem. Plagiarism detection based on target code can be used for protecting the security of source codes. In this paper, we propose a new plagiarism detection technique for Java programs using bytecodes without referring their source codes. The plagiarism detection procedure using bytecode consists of two major steps. First, we generate the token sequences from the Java class file by analyzing the code area of methods. Then, we evaluate the similarity between token sequences using the adaptive local alignment. According to the experimental results, we can find the distributions of similarities of the source codes and that of bytecodes are very similar. Also, the correlation between the similarities of source code pairs and those of bytecode pairs is high enough for typical test data. The plagiarism detection system using bytecode can be used as a preliminary verifying tool before detecting the plagiarism by source code comparison.

• Journal of the Korean Society for Precision Engineering
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• v.15 no.10
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• pp.65-72
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• 1998

The BTA drilling chip is better for deep hole drilling than other self-piloting with pad drilling chips because the large length to diameter ratio allows a unique cutting force dispersion and better supplies the high pressure fluid. Therefore the BTA is useful for many tasks, such as coolant hole drilling of large scale dies, as well as tube seat drilling, which is essential for the heat exchanger, and variable component drilling for automobiles. Deep hole drilling has several significant problems, such as hole deviation, hole over-size, circularity, straightness, and surface roughness. The reasons for these problems, which often result in quality short comings, are an alignment of the BTA drilling system and the unbalance of cutting force by work piece and tool shape. This paper analyzes the properties through an experiment which com¬pared single-edge BTA drills with multiple-edge BTA drills, as well as the shapes of the tools to cause an unbalance of cutting force, and its effect on the precision of the worked hole. Conclusions are as follows. 1) In SMSSC drilling, 60m/min of BTA with single and multi-edged tools proved the best cutting condition and the lowest wear character. 2) The roundness got a little worse as cutting speed was increased, but surface roughness was hot affected. 3) It was proved that the burnishing torque of both drills approached 26%. which is almost the same as the 24% insisted on by Griffiths, and the dispersion characteristic of the multi-edged BTA drill proved better than the single-edge BTA drill.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.35 no.6
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• pp.661-667
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• 2011

Kinematic contact trigger probes are widely used for feature inspection and measurement on coordinate measurement machines (CMMs) and computer numerically controlled (CNC) machine tools. Recently, the probing accuracy has become one of the most important factors in the improvement of product quality, as the accuracy of such machining centers and measuring machines is increasing. Although high-accuracy probes using strain gauge can achieve this requirement, in this paper we study the universal economic kinematic contact probe to prove its probing mechanism and errors, and to try to make the best use of its performance. Stylus-ball-radius and center-alignment errors are proved, and the probing error mechanism on the 3D measuring coordinate is analyzed using numerical expressions. Macro algorithms are developed for the compensation of these errors, and actual tests and verifications are performed with a kinematic contact trigger probe and reference sphere on a CNC machine tool.

• Journal of KSNVE
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• v.7 no.2
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• pp.231-238
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• 1997

An automated scanning laser Doppler vibrometer (LDV) has been designed, and built to measure in-plane vibration fields over structures. Use of optical fibers allows the compact design of a laser probe head which can be scanned over the vibrating structures. An algorithm for automated self-alignment of the laser probe is developed. The system is completely automated for scanning over the structures, focusing two laser beams at each data point until the detected vibration signal is stable, and for recording and transferring the data to a system computer. The automated system allows one to get extensive data of the vibration field over the structures. The system is tested by scanning a piezoelectric cylindrical shell and a plate excited by a continuous signal and by a pulse signal, respectively. Results show that the automated scanning LDV system can be a useful tool to measure the in-plane vibration field and to detect the elastic waves propagating on the vibrating structures.

• Journal of Crop Science and Biotechnology
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• v.10 no.4
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• pp.231-236
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• 2007

Differentially expressed genes(DEG) were identified in a rice variety, Sathi, an indica type showing high allelopathic potential against barnyardgrass(Echinochloa crus-galli(L.) Beauv. var. frumentaceae). Rice plants were grown with and without barnyardgrass and total RNA was extracted from rice leaves at 45 days after seeding. DEG full-screening was performed by $GeneFishing^{TM}$ method. The differentially expressed bands were re-amplified and sequenced, then analyzed by Basic Local Alignment Search Tool(BLAST) searching for homology sequence identification. Gel electrophoresis showed nine possible genes associated with allelopathic potential in Sathi, six genes(namely DEG-1, 4, 5, 7, 8, and 9) showed higher expression, and three genes(DEG-2, 3 and 6) showed lower expression as compared to the control. cDNA sequence analysis showed that DEG-7 and DEG-9 had the same sequence. From RT PCR results, DEG-6 and DEG-7 were considered as true DEG, whereas DEG-1, 2, 3, 4, 5, and 8 were considered as putative DEG. Results from blast-n and blast-x search suggested that DEG-1 is homologous to a gene for S-adenosylmethionine synthetase, DEG-2 is homologous to a chloroplast gene for ribulose 1,5-bisphosphate carboxylase large subunit, DEG-8 is homologous to oxysterol-binding protein with an 85.7% sequence similarity, DEG-5 is homologous to histone 2B protein with a 47.9% sequence similarity, DEG-6 is homologous to nicotineamine aminotransferase with a 33.1% sequence similarity, DEG-3 has 98.8% similarity with nucleotides sequence that has 33.1% similarity with oxygen evolving complex protein in photosystem II, DEG-7 is homologous to nucleotides sequence that may relate with putative serin/threonine protein kinase and putative transposable element, and DEG-4 has 98.8% similarity with nucleotides sequence for an unknown protein.

• The Journal of the Acoustical Society of Korea
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• v.24 no.8
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• pp.431-440
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• 2005

The precise measurement of ultrasonic power is important to the qualify assurance and the safety of medical ultrasonic equipments In the Present work, a brier theory was introduced to determine the ultrasonic Power from the time valving balance-indication due to the radiation force acting on an absorbing target and/or other causes such as buoyancy during the repetition of on/off behavior of ultrasonic irradiation. The developed automated system measuring the ultrasonic power was described in detail with the precise mechanical alignment tool , the electric signal generation network, the control and measurement network and the appropriate procedure. The ultrasonic power measured by the developed system was compared to the reference data calibrated by the other national metrology institute at 1 MHz, 5 MHz, 10 MHz, and 15 MHz over the range 10 mW to 10 W. Their relative differences are within $5\%$.

• International Journal of Industrial Entomology and Biomaterials
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• v.27 no.2
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• pp.219-227
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• 2013

The purpose of this study was to quickly and effectively identify the Nosema disease of bumblebees in Gangwon Province in Korea. Bumblebees are crucial pollinators of various crops, and microsporidia are the critical pathogens of these hosts. When bumblebees are infected with Nosema bombi, their abdomens can become distended. Paralyzed and infected workers often become sluggish and die early. We have identified the morphology of the microsporidium by light and electron microscopy, and found it to have fairly small oval spores, as has been described previously in many other articles. For the specific and sensitive diagnosis of the microsporidian parasite N. bombi in bumblebees, we have developed an improved method of the polymerase chain reaction (PCR) for expeditious diagnosis. Two pairs of primers were tested on N. bombi and the related microsporidia Nosema apis and Nosema sp., both of which infect Bombus ignitus and Bombus hypocrita sapporoensis. Furthermore, we have verified and analyzed the 16SrRNA sequence data of N. bombi in bumblebees by using the Basic Local Alignment Search Tool (BLAST) server at the National Center for Biotechnology Information.