Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
권호 표지
DOI QR코드

DOI QR Code

Five Rare Non-Tuberculous Mycobacteria Species Isolated from Clinical Specimens

임상에서 분리된 희귀 비결핵 마이코박테리아 5종

  • Park, Young-Kil (The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association) ;
  • Lee, Young-Ju (The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association) ;
  • Yu, Hee-Kyung (The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association) ;
  • Jeong, Mi-Young (The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association) ;
  • Ryoo, Sung-Weon (The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association) ;
  • Kim, Chang-Ki (The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association) ;
  • Kim, Hee-Jin (The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association)
  • 박영길 (대한결핵협회 결핵연구원) ;
  • 이영주 (대한결핵협회 결핵연구원) ;
  • 유희경 (대한결핵협회 결핵연구원) ;
  • 정미영 (대한결핵협회 결핵연구원) ;
  • 류성원 (대한결핵협회 결핵연구원) ;
  • 김창기 (대한결핵협회 결핵연구원) ;
  • 김희진 (대한결핵협회 결핵연구원)
  • Received : 2010.07.27
  • Accepted : 2010.10.06
  • Published : 2010.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Recently, the rate of infections with non-tuberculous mycobacteria (NTM) has been increasing in Korea. Precise identification of NTM is critical to determination of the pathogen and to target treatment of NTM patients. Methods: Sixty-eight unclassified mycobacteria isolates by rpoB PCR-RFLP assay (PRA) collected in 2008 were analyzed by National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST) search after sequencing of 16S rRNA, hsp65, rpoB genes. Results: Nineteen strains of 68 isolates were specified as species after sequencing analysis of 3 gene types. We found 3 M. lentifulavum, 5 M. arupense, 4 M. triviale, 4 M. parascrofulaceum, and one M. obuense. One M. tuberculosis and another M. peregrinum were mutated at the Msp I recognition site needed for rpoB PRA. The remaining 49 isolates did not coincide with identical species at the 3 kinds genes. Conclusion: Sequencing analysis of 16S rRNA, hsp65, rpoB was useful for identification of NTM unclassified by rpoB PRA.

Keywords

References

  1. Wolinsky E. Nontuberculous mycobacteria and assoassociated diseases. Am Rev Respir Dis 1979;119:107-59.
  2. Primm TP, Lucero CA, Falkinham JO 3rd. Health impacts of environmental mycobacteria. Clin Microbiol Rev 2004;17:98-106. https://doi.org/10.1128/CMR.17.1.98-106.2004
  3. Prince DS, Peterson DD, Steiner RM, Gottlieb JE, Scott R, Israel HL, et al. Infection with Mycobacterium avium complex in patients without predisposing conditions. N Engl J Med 1989;321:863-8. https://doi.org/10.1056/NEJM198909283211304
  4. Koh WJ, Kwon OJ, Yu CM, Jeon KM, Suh GY, Chung MP, et al. Recovery rate of nontuberculous mycobacteria from acid-fast-bacilli smear-positive sputum specimens. Tuberc Respir Dis 2003;54:22-32.
  5. Jeong J, Lee SH, Jeong US, Chang CL, Kim SR. Identification of mycobacteria using high performance liquid chromatography in clinical specimens. Korean J Clin Microbiol 2004;7:148-55.
  6. Koh WJ, Kwon OJ, Lee KS. Diagnosis and treatment of nontuberculous mycobacterial pulmonary diseases: a Korean perspective. J Korean Med Sci 2005;20:913-25. https://doi.org/10.3346/jkms.2005.20.6.913
  7. Lee H, Park HJ, Cho SN, Bai GH, Kim SJ. Species identification of mycobacteria by PCR-restriction fragment length polymorphism of the rpoB gene. J Clin Microbiol 2000;38:2966-71.
  8. Devulder G, Perouse de Montclos M, Flandrois JP. A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model. Int J Syst Evol Microbiol 2005;55:293-302. https://doi.org/10.1099/ijs.0.63222-0
  9. Telenti A, Marchesi F, Balz M, Bally F, Bottger EC, Bodmer T. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 1993;31:175-8.
  10. Kim BJ, Lee KH, Park BN, Kim SJ, Bai GH, Kim SJ, et al. Differentiation of mycobacterial species by PCRrestriction analysis of DNA (342 base pairs) of the RNA polymerase gene (rpoB). J Clin Microbiol 2001;39: 2102-9. https://doi.org/10.1128/JCM.39.6.2102-2109.2001
  11. Springer B, Wu WK, Bodmer T, Haase G, Pfyffer GE, Kroppenstedt RM, et al. Isolation and characterization of a unique group of slowly growing mycobacteria: description of Mycobacterium lentiflavum sp. nov. J Clin Microbiol 1996;34:1100-7.
  12. Lee ES, Lee MY, Han SH, Ka JO. Occurrence and molecular differentiation of environmental mycobacteria in surface waters. J Microbiol Biotechnol 2008;18:1207-15.
  13. Shin S, Kim EC, Yoon JH. Identification of nontuberculous mycobacteria by sequence analysis of the 16S ribosomal RNA, the heat-shock protein 65 and the RNA polymerase beta-subunit genes. Korean J Lab Med 2006;26:153-60. https://doi.org/10.3343/kjlm.2006.26.3.153
  14. Cloud JL, Meyer JJ, Pounder JI, Jost KC Jr, Sweeney A, Carroll KC, et al. Mycobacterium arupense sp. nov., a non-chromogenic bacterium isolated from clinical specimens. Int J Syst Evol Microbiol 2006;56:1413-8. https://doi.org/10.1099/ijs.0.64194-0
  15. Turenne CY, Cook VJ, Burdz TV, Pauls RJ, Thibert L, Wolfe JN, et al. Mycobacterium parascrofulaceum sp. nov., novel slowly growing, scotochromogenic clinical isolates related to Mycobacterium simiae. Int J Syst Evol Microbiol 2004;54:1543-51. https://doi.org/10.1099/ijs.0.02940-0
  16. Tortoli E, Chianura L, Fabbro L, Mariottini A, Martin- Casabona N, Mazzarelli G, et al. Infections due to the newly described species Mycobacterium parascrofulaceum. J Clin Microbiol 2005;43:4286-7. https://doi.org/10.1128/JCM.43.8.4286-4287.2005
  17. Molavi A, Weinstein L. In vitro susceptibility of atypical mycobacteria to rifampin. Appl Microbiol 1971;22:23-5.
  18. Lee CK, Gi HM, Cho Y, Kim YK, Lee KN, Song KJ, et al. The genomic heterogeneity among Mycobacterium terrae complex displayed by sequencing of 16S rRNA and hsp 65 genes. Microbiol Immunol 2004;48: 83-90. https://doi.org/10.1111/j.1348-0421.2004.tb03492.x
  19. Tsukamura M, Mizuno S. Numerical analysis of relationships among rapidly growing, scotochromogenic mycobacteria. J Gen Microbiol 1977;98:511-7. https://doi.org/10.1099/00221287-98-2-511
  20. Agusti G, Astola O, Rodríguez-Guell E, Julián E, Luquin M. Surface spreading motility shown by a group of phylogenetically related, rapidly growing pigmented mycobacteria suggests that motility is a common property of mycobacterial species but is restricted to smooth colonies. J Bacteriol 2008;190:6894-902. https://doi.org/10.1128/JB.00572-08
  21. Pace NR. A molecular view of microbial diversity and the biosphere. Science 1997;276:734-40. https://doi.org/10.1126/science.276.5313.734
  22. Thorne JL, Kishino H, Painter IS. Estimating the rate of evolution of the rate of molecular evolution. Mol Biol Evol 1998;15:1647-57. https://doi.org/10.1093/oxfordjournals.molbev.a025892
  23. Tortoli E. Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev 2003;16:319-54. https://doi.org/10.1128/CMR.16.2.319-354.2003
  24. Park Y, Shin HB, Kim CK, Roh KH, Yum JH, Yong D, et al. Identification of bacterial and fungal isolates by sequence analysis of 16S rRNA and internal transcribed spacer. Korean J Clin Microbiol 2010;13:34-9. https://doi.org/10.5145/KJCM.2010.13.1.34
  25. Cloud JL, Neal H, Rosenberry R, Turenne CY, Jama M, Hillyard DR, et al. Identification of Mycobacterium spp. by using a commercial 16S ribosomal DNA sequencing kit and additional sequencing libraries. J Clin Microbiol 2002;40:400-6. https://doi.org/10.1128/JCM.40.2.400-406.2002
  26. Ryoo SW, Shin S, Shim MS, Park YS, Lew WJ, Park SN, et al. Spread of nontuberculous mycobacteria from 1993 to 2006 in Koreans. J Clin Lab Anal 2008;22: 415-20. https://doi.org/10.1002/jcla.20278