• Research in Plant Disease
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• v.18 no.4
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• pp.298-303
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• 2012

Tomato yellow leaf curl virus (TYLCV), transmitted exclusively by the whitefly (Bemisia tabaci) in a circulative manner is one of the most important virus in tomato. Since the first report of TYLCV incidence in Korea in 2008, the virus has rapidly spread nationwide. TYLCV currently causes serious economic losses in tomato production in Korea. Early detection of TYLCV is one of the most important methods to allow rouging of infected tomato plants to minimize the spread of TYLCV disease. We have developed an ultra-rapid and sensitive real-time polymerase chain reaction (PCR) using a new designed real-time PCR system, GenSpectorTM TMC-1000 that is a small and portable real-time PCR machine requiring only a $5{\mu}l$ reaction volume on microchips. The new system provides ultra-high speed reaction (30 cycles in less than 15 minutes) and melting curve analysis for amplified TYLCV products. These results suggest that the short reaction time and ultra sensitivity of the GenSpector$^{TM}$-based real-time PCR technique is suitable for monitoring epidemics and pre-pandemic TYLCV disease. This is the first report for plant virus detection using an ultra-rapid real-time PCR system.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.563-570
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• 2014

The shortage or surplus of minerals directly affects overall physiological metabolism of plants; especially, it strongly influences carbohydrate metabolism as a primary response. We have studied mineral uptake, synthesis and partitioning of soluble carbohydrates, and the relationship between them in N, P or K-excessive tomato plants, and examined the interaction between soluble carbohydrates and mineral elements. Four-weeks-old tomato plants were grown in a hydroponic growth container adjusted with excessive N ($20.0mmol\;L^{-1}$ $Ca(NO_3)2{\cdot}4H_2O$ and $20.0mmol\;L^{-1}$ $KNO_3$), P ($2.0mmol\;L^{-1}$ $KH_2PO_4$), and K ($20.0mmol\;L^{-1}$ $KNO_3$), respectively, for 30 days. Shoot growth rates were significantly influenced by excessive N or K, but not by excessive P. The concentrations of water soluble N (nitrate and ammonium), P and K were clearly different with each tissue of tomato plants as well as the mineral conditions. The NPK accumulation in all treatments was as follows; fully expanded leaves (48%) > stem (19%) = roots (16%) = petioles (15%) > emerging leaves (1). K-excessive condition extremely contributed to a remarkable increase in the ratio, which ranged from 2.79 to 10.34, and particularly potassium was dominantly accumulated in petioles, stem and roots. Fresh weight-based soluble sugar concentration was the greatest in NPK-sufficient condition ($154.8mg\;g^{-1}$) and followed by K-excessive (141.6), N-excessive (129.2) and P-excessive (127.7); whereas starch was the highest in K-excessive ($167.0mg\;g^{-1}$) and followed by P-excessive (146.1), NPK-sufficient (138.2) and N-excessive (109.7). Soluble sugar showed positive correlation with dry weight-based total N content (p<0.01) whereas was negatively correlated with soluble P (p<0.01) and dry weight-based total P (p<0.01). On the other hand, starch production was negatively influenced by total N (p<0.001), but, it showed positive relation with total K concentration (p<0.05). This study shows that uptake pattern of NPK and production and partitioning of soluble carbohydrate were substantially different from each mineral, and the relationship between water soluble- and dry weight-based-mineral was positive.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.414-423
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• 2016

Growers in plug seedling production think that root media in which rockwool is a component has given rise to several environmental problems. Therefore, the demand for new materials as a substitute for rockwool has been increased. This study examined the possibility of cultivation of tomato plug seedlings using a newly developed growing medium with phenolic foam. Plug seeds of tomato cultivar 'Madison' were sown in four pellet-type growing media: Grodan rockwool (GRW), UR rockwool (URW), phenolic foam LC (LC) or phenolic foam LC-lite (LC-lite). Then, the seedlings were transplanted to the four cube-type growing media 19 days after sowing. Seeds were germinated in a growth chamber ($25{\pm}2^{\circ}C$, 80% relative humidity, and dark) for 4 days and then the seedlings were grown with a nutrient solution supplied by an overhead irrigation system in a greenhouse. Plant height, number of leaves, leaf area, and fresh or dry weight of tomato seedlings were the greatest for the seedlings transplanted to URW cube media after being grown on LC-lite pellets. Root grade was the greatest for the seedlings transplanted on LC or LC-lite cube media after being grown on LC pellets. Chemical properties of all media tested for tomato growth were maintained within a stable range, while physical properties of URW showed high values in container capacity, air space, and total porosity. These results demonstrated that the phenolic foam media were effective for seedling growth and can substitute for rockwool as a root medium.

• Journal of Bio-Environment Control
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• v.16 no.2
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• pp.108-114
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• 2007

This study was conducted to clarify the effects of the different mixing ratios of substrate mixtures based on peat moss and the concentration of nutrient solution on the growth of tomato (Lycopersicon esculentum Mill.) seedlings. Substrates such as peat moss, rice hull, carbonized rice hull, decomposed sawdust, perlite and granular rock wool were mixed and used. The concentration of nutrient solution were adjusted to EC $0.5{\sim}1.5mS/cm$. The volumetric moisture contents became higher as peat moss mixed were much more. Total porosities in all substrate mixtures were over 80%, and pH in substrate mixtures became lower as the volume of peat moss mixed higher. Mixing ratios of substrates suitable for the production of tomato seedlings with the higher quality were peat moss:rice hull:carbonized rice hull:decomposed sawdust:perlite=25:10:25:20:20(v/v). The plant growth was not significant among the different substrate mixtures. However, plant growth such as plant height, leaf area, and total dry weight became significantly increased as EC increasing.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1996.05a
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• pp.63-73
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• 1996

The importance of plant factory which blend agriculture and manufacture has been discussed widely. This company has researched hydroponics technique for cultivation which has been mainly developed in the sites of Okinawa and Hyougo Prefecture. In 1987, we stopped the previous research there, and started a new one far plant factory at the Agricultural Research Center of Chiba Prefecture. In 1989, we built four experimental plant factories (120㎡) at Chiba Oil Factory Refinery in Ichihara-city, developing techniques of cultivation, equipment production and environmental control for cultivation system of tomato, strawberry and leaf vegetables. (omitted)

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.9
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• pp.1470-1476
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• 2020

Objective: This study was carried out to investigate physicochemical properties, and antioxidant and antimicrobial activities of low-fat sausages (LFSs) covered with sodium alginate (SA) film alone and with powder film (TSA-film) formed by cross-linking cherry tomato powder (CTP) and SA with calcium chloride (CaCl₂). Methods: Sausages covered with the biodegradable film were assessed based on the measurement of pH, color (L^⋆, a^⋆, b^⋆), proximate analysis, expressive moisture (EM), texture profile analysis, total plate counts (TPC), violet red bile, and 2-Thiobarbituric acid reactive substances (TBARS) during storage under refrigeration. LFSs wrapped with TSA-film were compared with those wrapped with SA-film and without film (control) during storage at 10℃ for 35 days. Results: The LFSs covered with the mixed film had lower pH, lightness (L^⋆), EM%, TBARS, and TPC, but lower yellowness (b^⋆) and hardness values than those wrapped with TSA-film alone. Conclusion: Lipid oxidation and microbial growth was retarded in sausages covered with biodegradable films, especially multiple films as compared to single film, thereby resulting in extended shelf-life of the LFSs.

• Journal of Korean Society for Quality Management
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• v.49 no.4
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• pp.569-579
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• 2021

Purpose: The purpose of this study is the time series analysis for predicting the yield of crops applicable to each farm using environmental variables measured by smart farms cultivating tomato. In addition, it is intended to confirm the influence of environmental variables using a deep learning model that can be explained to some extent. Methods: A time series analysis was performed to predict production using environmental variables measured at 75 smart farms cultivating tomato in two periods. An LSTM-based encoder-decoder model was used for cases of several farms with similar length. In particular, Dual Attention Mechanism was applied to use environmental variables as exogenous variables and to confirm their influence. Results: As a result of the analysis, Dual Attention LSTM with a window size of 12 weeks showed the best predictive power. It was verified that the environmental variables has a similar effect on prediction through wieghtss extracted from the prediction model, and it was also verified that the previous time point has a greater effect than the time point close to the prediction point. Conclusion: It is expected that it will be possible to attempt various crops as a model that can be explained by supplementing the shortcomings of general deep learning model.

• Journal of Plant Biotechnology
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• v.7 no.2
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• pp.87-95
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• 2005

The tomato ripening associated membrane protein (TRAMP) (Fray et al., 1994) is a member of the major intrinsic protein (MIP) family, defined as channels facilitating the passage of water and small solutes through membranes. During normal fruit ripening the TRAMP mRNA levels were increased whereas the expression levels of TRAMP in low ethylene ACO1-sense suppressed lines, Nr and rin fruits, were lower than at the breaker stage of wild type fruit. TRAMP mRNA is inhibited by $LaCl_3$, which is an inhibitor of $Ca^{2+}$-stimulated responses, treatment but drought condition did not affect TRAMP expression. The levels of TRAMP mRNA transcripts were substantially higher in the dark treated seedlings and fruits. These suggest that TRAMP function as a water channel may be doubted because of several reasons; no water content was changed during ripening in wild type, antisense and overexpression lines, TRAMP expression under light condition was lower than dark condition and TRAMP expression was not changed in drought condition. Co-suppression plant, 3588 was one of sense suppression lines, which contain CaMV 35S promoter and sense pNY507 cDNA, produced small antisense RNA, approximately 21-25 nucleotides in length, mediated post-transcriptional gene silencing. Therefore, TRAMP expression was inhibited by small antisense and multiple copies might induce gene silencing without any production of double strand RNA. Total seven selected volatile productions, isobutylthiazole, 6-methyl-5-hepten-2-one, hexanal, hexenal methylbutanal, hexenol, and methylbutanol, were highly reduced in sense line whereas total volatile production was increased in TRAMP antisense line. These results suggested TRAMP might change volatile related compounds.

• Journal of Environmental Science International
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• v.32 no.12
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• pp.973-978
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• 2023

This study determined the mechanisms of selection of companion plants that will increase natural enemies and compared and analyzed the effect of suppression of pest density and changes in pest and natural enemy density and spatial distribution, aiming to select suitable companion plants to control major pests that are problematic in organic tomato facility cultivation. As a result of the companion plant selection, 13.5 days were identified in the area with daily flowers among five species of flowering plants. In the experiment to determine the timing of natural enemies, the best results were found in the treatment group introduced two weeks before the pest occurred. As a result of the actual package test, farmers could see that the density of greenhouse pollen decreased significantly (100-500% for adults and 11-67% for larvae compared to no treatment) in the treatment with companion plants. Based on the results of this study, we expect that ecological pest management using companion plants that attract natural enemies will help to increase biodiversity through vegetation management, secure the safe production of organic products and improve the sustainability of agriculture.

• Journal of Korean Society of Rural Planning
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• v.29 no.4
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• pp.127-135
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• 2023

This study examined the impact of changes in agricultural production methods on society, the economy, and the environment. While traditional open-field farming relied heavily on natural conditions, modern approaches, including greenhouse and smart farming, have emerged to mitigate the effects of climate and seasonal variations. Facility horticulture has been on the rise since the 1990s, and recently, there has been a growing interest in smart farms due to reasons such as climate change adaptation and food security. We compared open-field spinach and greenhouse spinach using agricultural income survey data, and we also compared greenhouse tomato cultivation with smart farming tomato cultivation, utilizing data from the smart farm survey reports. The economic results showed that greenhouse spinach increased yield by 25.8% but experienced a 29% decrease in income due to equipment depreciation. In the case of tomato production in smart farms, both yield and income increased by 36-39% and 34-46%, respectively. In terms of environmental impact, we also compared fertilizer and energy usage. It was found that greenhouse spinach used 29% less fertilizer but 14% more energy compared to open-field spinach. Smart farming for tomatoes saw a negligible decrease in electricity and fuel costs. Regarding the social impact, greenhouse spinach reduced labor hours by 31%, and the introduction of smart farming for tomatoes led to an average 11% reduction in labor hours. This reduction is expected to have a positive effect on sustainable farming. In conclusion, the transition from open-field to greenhouse cultivation and from greenhouse cultivation to smart farming appears to yield positive effects on the economy, environment, and society. Particularly, the reduction in labor hours is beneficial and could potentially contribute to an increase in rural populations.