• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2023년도 춘계학술대회
• /
• pp.156-156
• /
• 2023

Salinity is a major abiotic stress that limits rice productivity in many regions of the world. In order to develop salt stress tolerant rice plants, genetic engineering is a promising approach. We characterized the molecular function of rice C3H2C3 as a really interesting new gene (RING). Oryza sativa RING finger protein H2-3 (OsRFPH2-3) was highly expressed in 100 mM NaCl. To identify the localization of OsRFPH2-3, we fused vectors that include C-terminal GFP protein (35S;;OsRFPH2-3-GFP). OsRFPH2-3 was expressed in the nucleus in rice protoplasts. An in vitro ubiquitin assay demonstrated that OsRFPH2-3 possessed E3-ubiquitin ligase activity. However, the mutated OsRFPH2-3 were not possessed any E3-ubiquitin ligase activity. Under salinity conditions, OsRFPH2-3-overexpressing plants exhibited higher chlorophyll, proline, SOD, POD, CAT, and soluble sugar contents and lower H₂O₂ accumulation than wild-type plants, supporting transgenic plants with enhanced salinity tolerance phenotypes. OsRFPH2-3-overexpressing plants exhibited low Na+ accumulation and Na+/K+ ratios in their roots. Theses results suggest that overexpression of OsRFPH2-3 can make plant insensitivity about salinity conditions.

• 한국잡초학회지
• /
• 제16권2호
• /
• pp.150-159
• /
• 1996

Oxyfluorfen 내성(耐性) 및 감수성(感受性) 벼품종(品種)과 감수성 피를 공시하여 oxyfluorfen 처리에 의한 protox 활성저해(活性沮害), PPIX 축적량(蓄積量)과 tetrapyrro1e 생합성(生合性) 억제제(抑制齊) 처리에 의한 oxyfluorfen의 제초활성(除草活性) 억제효과를 조사하였다. 1. Oxyfluorfen 처리에 따른 protox 활성저해(活性沮害)는 내성(耐性) 벼품종(品種)들보다 감수성(感受性) 벼품종(品種)들과 피에서 컸다. 특히,$10^{-6}$ 처리 이상의 농도에서 감수성(感受性) 벼품종(品種)들과 피에서는 완전히 억제되었으나, 내성(耐性) 품종(品種)들에서는 무처리에 대비하여 32~59%의 활성(活性)을 유지하였다. 2. 처리농도가 증가할수록 PPIX의 축적량(蓄積量)은 증가하였고, 광노출 4시간까지는 증가하나 그 이후에는 감소하는 경향이었다. 3. Oxyfluorfen 처리에 의한 PPIX 축적량(蓄積量)은 암조건(暗條件)에서보다 광조건(光條件)에서 많았다. 광 암조건(光 暗條件)에서 감수성(感受性) 벼품종(品種)들과 피는 내성(耐性) 벼품종(品種)들에 비하여 PPIX 축적량(蓄積量)이 많았다. 특히, 감수성(感受性)인 피는 PPIX 축적량(蓄積量)이 벼품종(品種)에 비해 많았다. 4. Tetrapyrrole 생합성(生合性) 억제제(抑制齊)인 GC와 DA를 처리하면 oxyfluorfen에 의한 제초활성(除草活性)은 억제되었으며, 내성(耐性) 벼품종(品種)보다는 감수성(感受性) 벼품종(品種)이나 피에서 그 효과가 떨어지는 경향으로서 엽록소 함량이나 PPIX 축적량(蓄積量)에서도 동일한 경향을 나타내었다.

• Journal of Plant Biotechnology
• /
• 제42권4호
• /
• pp.409-413
• /
• 2015

본 연구는 토양에 오염된 중금속을 제거하기 위한 식물정화공정에 사용할 식물체를 개발하기 위해 $TgMTP_1$ 유전자를 CaMV35S 상시 발현 할 수 있도록 Ti-plasmid 벡터를 구축하여 형질전환식물을 육성하였다. 유전자가 도입된 형질전환 애기장대에서 TgMTP유전자를 과발현하는 호모 TG-116 (T3 generation) 계통을 육성하여 그 특성을 조사하였다. 호모계통으로 육성한 TG-116 계통은 callus 및 식물체에서 중금속에 대한 저항성을 보였다. 특히 RT-PCR 및 Western 분석에서 유전자의 발현은 잎에서 높게 나타났으며, Ni 흡수 및 축적이 많이 일어났다. 따라서 MTP1 유전자가 발현되어 액포에 중금속을 축적하는 실험결과를 활용한다면 식물정화공정에 사용할 수 있는 다양한 유전자원으로 기대할 수 있다.

• 한국작물학회지
• /
• 제45권1호
• /
• pp.26-31
• /
• 2000

This study was carried out to obtain the basic information for breeding cold-tolerant rice varieties with high yield-productivity through wide crosses between indica and japonica rice. Genetic analysis was conducted using 55 F$_1$s obtained from half-diallel crosses among eleven cultivars of various origin including indica and japonica rice. Screening for cold tolerance was done with cold-water irrigation after transplanting until ripening stage. Both general combining ability (GCA) and specific combining ability (SCA) effects were highly significant in all characters associated with dry matter accumulation at 30 and 50days after cold-water irrigation (DAC). The variance of GCA was much larger than that of SCA in plant height, shoot dry weight per plant (DWP), crop growth rate (CGR) and cold-water response index (CRI) of these characters except CRI of shoot dry weight per plant. The DWP, CGR and CRI of these characters of Gaochan 102, Tong88-7 and TR22183 were markedly higher than those of the others. GCA effects of these varieties on DWP, CGR and their CRI were also higher than those of the others, indicating that they are useful as promising parents for breeding cold-tolerant varieties. Analysis of genetic parameters for 11$\times$11 half-diallel F$_1$s revealed that inter-locus gene interaction were concerned in the expression of plant height at 50 DAC, CRI of DWP at 50 DAC, and CRI of CGR, and that intra-locus gene interaction for plant height and the other characters were partial dominance and over-dominance, respectively. Narrow-sense heritability (h$^2$$_{N}$) was the highest in plant height as 0.729, and the lowest in CRI of DWP at 30 DAC as 0.048, suggesting that selection for cold tolerance will be quite effective in case that the selection criterion is the performance itself.f.

• 동의생리병리학회지
• /
• 제24권4호
• /
• pp.646-652
• /
• 2010

Gambejaeseup-tang (GBJST) have recently been used as an anti-obesity herbal medicine but their effect and mechanism of action have not been studied. We modified ingredients of GBJST based on the previous experiments about exploring herbs to suppress triglyceride accumulation in 3T3-L1 adipocytes. We investigated the effects of modified GBJST on energy, glucose and lipid homeostasis using female rats with diet-induced obesity and their action mechanism was also determined. Rats fed a high-fat diet (HFD) were divided into 3 groups: rats in each group received 0.2 or 2 g water extracts of modified GBJST (L-GBJST or H-GBJST) or 2 g cellulose per kg body weight (a negative control) on a daily basis. A further group was fed a low-fat diet (LFD) as a positive control. We found that modified GBJST dose-dependently decreased body weight and mesenteric and retroperitoneal fat more than the control. This decrease was due to the reduction in energy intake and the increase of energy expenditure. HFD increased fat oxidation more than LFD and modified GBJST further increased fat oxidation as a major energy source more than the control in a dose-dependent manner. In addition, H-GBJST improved glucose tolerance without changing serum insulin levels during an oral glucose tolerance test. H-GBJST also suppressed the increase of serum total and LDL cholesterol and triglyceride levels by HFD. In conclusion, modified GBJST have a good anti-obesity effect by decreasing energy intake and increasing energy expenditure mainly as fat in female rats with diet-induced obesity. It also improves glucose tolerance and lipid metabolism.

• 한국작물학회지
• /
• 제48권1호
• /
• pp.20-24
• /
• 2003

Proline is known as an osmotrotectant to enhance tolerance against both salt and dehydration stresses. A P5CS ($\Delta^1$-pyrroline-5-carboxylate synthetase) plays a major role in regulation of synthesis of proline. An overexpression of the mothbean P5CS gene in transgenic tobacco plant increased the levels of proline and osmotolerance. In an attempt to look for the possibility to use content of proline as well as a level of P5CS gene expression as molecular markers for salt tolerance, the amounts of proline and transcript levels of P5CS were measured as functions of either concentration of NaCl or length of treatment period among different species of zoysiagrass. Hybridzoysia showed the highest level of proline ($329\mu\textrm{g}$/g.f.w.) among five different species of zoysiagrass at 250 mM NaCl in 24 hours. The level of P5CS transcript was also the highest in the hybridzoysia at 250 mM NaCl in 24 hours. The transcriptions of P5CS gene were induced at the rates of 1.2, 1.2, 1.8, and 1.8, upon treatment of 250 mM NaCl in Z. japonica, Z. matrella, Z. sinica and hybridzoysia respectively. Based on a correlation between the level of P5CS transcript and the proline content among different species of zoysiagrass, a comparative structural analysis of the gene for P5CS from either Z. sinica or hybridzoysia may lead to an understanding of mechanism for salt tolerance shown differently among zoysiagrasses.

• 원예과학기술지
• /
• 제32권5호
• /
• pp.684-693
• /
• 2014

저온, 건조, 염과 같은 비생물적 스트레스는 식물의 생리적 형태적 변화와 수확량 감소를 초래한다. 이러한 이유로 식물체는 불리한 환경을 극복하기 위해 다양한 대사과정에 관련된 유전자들간의 복잡한 상호 관계를 조절함으로써 저항성을 획득한다. 본 연구는 배추에서 염 스트레스에 반응하는 유전자를 다각적으로 분석하기 위해 상호발현 네트워크를 구축하였다. 네트워크를 구축하기 위하여 배추를 염스트레스 조건 하에서 시간 경과에 따라 KBGP-24K 마이크로어레이 분석을 실시한 [BrEMD (Brassica rapa EST and Microarray Database)] 실험 결과를 수집하여 분석하였다. 구축된 네트워크 모델은 1,853개 node, 5,740개 edge, 및 142개 connected component(상관계수 > 0.85)로 구성되었다. 구축된 네트워크 분석 결과, ROS 신호 전달을 통한 N$Na^+$ 수송활성화와 proline 축적이 배추의 염 저항성 획득과 밀접한 연관이 있는 것으로 판단하였다.

• 한국농림기상학회지
• /
• 제6권3호
• /
• pp.204-209
• /
• 2004

Cd 처리에 대한 박달나무의 반형매 차대간의 Cd 흡수 및 이동 패턴을 알아보고자 수행한 결과, 잎의 주간내 위치에 따른 Cd농도는 지제부에서 높게 나타나고 정단부로 올라 갈수록 농도가 낮아지는 경향을 보였다. 대체로 식물체내에서 Cd의 농도 분포는 뿌리>줄기>잎의 순으로 나타났지만, 가계별, 처리별로 상이한 결과가 나타나 가계간 유전적인 요인이 크게 작용한 것으로 판단된다. 모든 가계의 처리구에서 지상부와 지하부의 Cd 농도 비율이 1.26으로 나타나 박달나무는 Cd을 흡수하여 지상부로 보내는 축적종의 경향을 갖고 있는 것으로 판단된다. 또한 토양 중금속 농도와 식물체내 중금속 농도의 비도 가계간 차이는 있지만, Cd을 능동적으로 흡수하여 지상부로 이동시키는 Cd 정화 수종으로 판단된다. 하지만 박달나무는 Cd에 대한 반응이 가계간 다양하므로 토양 중 Cd정화를 위해 이용할 경우, 정화 능력이 우수한 수종을 선발하는 것뿐만 아니라, 같은 수종 내에서도 내성 능력과 정화능력이 차이가 있으므로 가계 선발이 필요할 것이다.

• Plant Biotechnology Reports
• /
• 제5권4호
• /
• pp.323-329
• /
• 2011

MuSI, a gene that corresponds to a domain that contains the rubber elongation factor (REF), is highly homologous to many stress-related proteins in plants. Since MuSI is up-regulated in the roots of plants treated with cadmium or copper, the involvement of MuSI in cadmium tolerance was investigated in this study. Escherichia coli cells overexpressing MuSI were more resistant to Cd than wild-type cells transfected with vector alone. MuSI transgenic plants were also more resistant to Cd. MuSI transgenic tobacco plants absorbed less Cd than wild-type plants. Cd translocation from roots to shoots was reduced in the transgenic plants, thereby avoiding Cd toxicity. The number of short trichomes in the leaves of wild-type tobacco plants was increased by Cd treatment, while this was unchanged in MuSI transgenic tobacco. These results suggest that MuSI transgenic tobacco plants have enhanced tolerance to Cd via reduced Cd uptake and/or increased Cd immobilization in the roots, resulting in less Cd translocation to the shoots.

• Journal of Plant Biotechnology
• /
• 제45권4호
• /
• pp.357-363
• /
• 2018

The objective of this study was to investigate whether exogenous proline (Pro) could confer freezing tolerance of spinach and determine fluctuations of free amino acids in spinach leaf tissues under freeze-induced stress. Treatment with Pro (10 mM) resulted in more accumulation of Pro (~2.6-fold) in Pro-treated spinaches compared to untreated ones. These Pro-pretreated spinaches were more freezing-tolerant, showing more turgid leaves and petioles compared to untreated controls. However, when spinaches pre-treated with or without Pro were subjected to freezing, there was no significant difference in overall amino acid contents, emphasizing the role of Pro as an osmoprotectant. Freezing stress prompted intensification of total amino acid contents irrespective of pretreatment with Pro. Asp, Glu, Ala, and Val were the most abundant free amino acids due to increased protein degradation and nitrogen mobilization for plant survival under freezing stress. Arg, a precursor for the synthesis of polyamines in plants, was profoundly enhanced under freezing stress. This implies that Arg plays an important role in modulating freezing tolerance. Gly, Leu, and Ile were maintained at relatively low levels in all treatments. However, Ser, Tyr, and Lys as primary constituents of dehydrins were accumulated under freezing stress, suggesting that they might play a role in increasing cryoprotective activity under freezing stress.