• Proceedings of the Ginseng society Conference
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• 1990.06a
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• pp.175-189
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• 1990

Nitrogen compounds of Panax ginseng and their biological activities in plant and animal were reviewed. Major nitrogen compounds found in P. ginseng are free amino acids. Water solilble proteins, indouble proteins and peptides. Minor nitrogen compounds are dencichine. Glycolyroteins, amines, alkaloides, methoxy or alkyl pyrazine derivatives, free nucleosides and nucleic acid bases. 4-methyl-i-thiazoltethanol and pyroglutamic acid the contents of total nitrogen and protein in root Increased until 13 years old which was the highest age tinder investigation. Soluble protein content increased with the root weight and was higher in xylem pith than cortex-epidermis indicating the close relation with root growth. Arginine, which covered 58% of total free amino acids, may serve as storage nitrogen. Arginine seems to be changed into proline in rhizome. threonine in stem and again threonine and arginine in leaf. The greater the root weight the higher the polyamine stimulated Polyamine stimlllated the growth of root callus. Physiological roles of other minor nitrogen compounds are unknown although content is relatively high ((1.if) 6.w). Biochemical and pharmacological activities of some nitrogen compounds for animal were more investigated than physiological role there plant itself. Radiation and U.V protective function (heat stable protein). insulin-like activity in lipogenesis and livolysis (adenosine and pyroglutamic acid), depression of blood sugar content (glycopevtide). htmostatic and nellrotoxic activity (dencichine) and, sedative and hypnotic activity (4-methyl-i-thiazoleethanol) are reported. Heat stable protein increased with root age. The traditional quality criteria appear to be well in accordance with biological activities of nitrogen compounds. Chemical studies of nitrogen compounds seem relatively rare, probably due to difficulty of isolation, subsequently the investigations of biological activities are little.

• Journal of Ginseng Research
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• v.14 no.2
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• pp.317-331
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• 1990

Nitrogen compounds of Panax ginseng and their biological activities in plant and animal were reviewed. Major nitrogen combounds found in P. ginseng are free amino acids, Water soluble teins, insoluble proteins and peptides. Minor nitrogen compounds are dencichine. glycol)roteins. amines, alkaloides, methoxy or alkyl pyrazine derivatives. free nucleosides and nllrleir arid bases. 4-me- thymi-5-thiazoleethanol and pyroglutamic acid. The contents of total nitrogen and protein in root increased until 13 years old rvhich was the highest age tinder investigation. Soluble protein content increased With the root weight and was higher in xylem pith than cortex-epidermis indicating the rlosc relation with root growth. Arginine which covered 58% of total free amino aroids may serve as a storage nitrogen. Arginine seems to be changed into proline in rhizome, threonine in stem and again threoning and arginine in leaf. The greater the root weight the higher the polyaminc content. Polyamine stimulated the growth of root callus. Physiological roles of other minor nitrogen compounds are unknown although dencichine content is relatively high (0.5% d.w.). biochemical and pharmatological activities of some nitrogen compounds for animal were more investigated than physiological roll iota plant itself. Radiation and U.V. protective function (heat stable protein), insulin-like activity in lipogenesis and lipolysis (adenosine and pyroglutamic acid), depression of blood sugar content (glycopeptide). hemostatir and nellrotoxic activity (denrichine) and. sedative and hypnotic activity (4-methyl-5-thiazoleethilnol) are reported. Heat stable protein increased with root age. The traditional quality critsria appear to be well in accordance with biological activities of nitrogen compounds. Chemical stlldies of nitrogen compounds seem relatively rare, probably dole to difficulty of isolation, subsequently the investigations of biological activities are little.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.3
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• pp.312-317
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• 1985

Growth response and nutrients uptake of tobacco grown on mulched and non mulched plots were investigated during the growing period. 1. The whole growing time was about 25 days shorter and relative growth rate was faster in the mulched plot than in the non mulched plot. 2. Nitrate content of leaf in the latter stage of tobacco grown on the non mulched plot was more continuously increased compared with mulched plot. As a result in the latter stage of tobacco leaf nitrate form of nitrogen level was much higher on non mulched plot than on mulched plot. 3. Reducing sugar content in cured leaves of tobacco grown on the non mulched plot was lower that of the mulched plot. This difference in reducing sugar content was remarkable in the bottom leaves that nitrate content was highest. 4. Tobacco growing on nonmulched soil may cause to be low quality owing to increasing of nitrogen compound and decreasing of reducing sugar content in cured leaves. 5. As distributions of nitrate and potassium content in leaves at different stalk position was similar each other, nitrate and potassium ion considered to be counter ion in absorption and translocation through the tobacco plant.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.2
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• pp.146-150
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• 2011

A large amount of nitrogenous compounds are found in burley tobacco and are responsible for peculiar aroma during smoking. Excess in nitrogen or its compounds such as total nitrogen and proteins in burley tobacco gives a strong pungency and make its taste bad. The present study has focused on improving quality of Burley tobacco by regulating topping and harvesting time of leaf to reduce nitrogen compounds in Burley tobacco. In addition, the early-stage of flower topping had lower level of nitrogenous compound, such as total nitrogen and protein nitrogen, and total tobacco-specific nitrosamine (TSNA) contents compared to the button-stage and full flower-stage topping. Also, the contents of nicotine got significantly decreased while the ether extract was increased as the period of topping got delayed. Regarding the brightness among the colors of cured leaves, the color at the full flower-stage topping was the brightest, and there was no particular difference in red and yellow colors. The harvesting amount was decreased as the period of topping got delayed and there was no significant difference between the topping treatments in terms of the exterior quality. As the harvesting time delayed, the total nitrogen, protein nitrogen, nitrate nitrogen, and ammonium nitrogen were decreased while the nicotine contents got increased. Also, the total TSNA contents were decreased as the harvesting time delayed. However, ether extract was increased. Among the colors of cured leaves, the brightness and degree of yellow color were significantly increased as the harvesting time delayed, and there was no particular difference in the degree of red color. The harvesting amount was decreased along with the delayed harvesting time; however, the harvest of the matured and mellow leaves was higher in terms of price, compared to that of the immature leaves. For the reduction of nitrogenous compound and TSNA contents in Burley tobacco, the topping was examined to be more effective in terms of improvement in chemical contents and quality regardless of the decreased harvesting amount.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.3
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• pp.310-316
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• 1987

A field experiment was conducted to find out the effects of liming(soil pH) and sources of N on growing characters and yield of burley tobacco. Treatments consisted of liming(nonliming, liming to soil pH5.5 and 6.5) as the main plot and N sources(compound fertilizer of containing 3.9% NH$_4$-N and 6.1% NH$_2$-N, NaNO$_3$,(NH$_2$)$_2$CO and (NH$_4$)$_2$SO$_4$as the sub-plot. The growth of vegetative growing stage of limed plots were delayed(to compare the nonlimed plot) by influence of alkali. When the source on N was NaNO$_3$ the growth of vegetative growing stage was unfavorable and the yellowing of lower leaves of maturing stage was rapid. The yield and value of cured leaf was increased by increasing the rate of Ca(OH)$_2$, but there was no significant differences among the source of N. The yield response to liming was greater when the source of N was (NH$_4$)$_2$SO$_4$ than that of any other plots.

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.255-260
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• 1996

This study was conducted to investigate the characteristics of aboveground plants and red ginseng of polystem ginseng in 6 years of age having two or more stems in a plant. Total leaf weight and area of polystem ginseng were larger, while its stem diameter, and the leaf weight and area of the big­gest stem in each plant were decreased with increase the stem numbers in a plant. The ratio of shoot weight to root weight in the polystem ginseng with three or more stems was higher than that in the monos­tem ginseng and the polystem ginseng with two stems, In ginseng plants with no more than 2 stems, there were positive correlations between root weight and total leaf weight, and leaf area, but not between leaf weight and area of the biggest stem. Inner cavity and inner white, limiting factors for redginseng quality grade, occurred more in tri-stem ginseng than mono- and di-stem one. Percentages of Heaven (1st grade) and Earth (2nd grade) red ginseng in tri-stem ginseng were decreased compared with mono stem and di-stem ginseng.

• Journal of Sericultural and Entomological Science
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• v.20 no.2
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• pp.26-31
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• 1978

The objective of the present study was to investigate the effect of nicotine-contaminated mulberry-leaf, which was grown in the vicinity of tobacco drying plant (TDP), on cocoon crop. Mulberry-leaf harvested from the field at Sericultural Experiment Station (SES), Suweon, Korea, was used as control, supposedly nicotine-free leaf. Leaf harvested from the field in the distances of 30-50m, 300-400m and 700-800m from TDP was fed during the whole larval stage of the silkworm at. SES. The effect of leaf in each treatment level on the quantitative characters of the silkworm was summarized as follows; 1. Larval duration from 4th instar on was significantly longer in the TDP-leaf treatments than for the control. 2. Duration of matured silkworm appearance became longer as the distance of the mulberry-field from TDP got shorter, because the larval duration and growth of the silkworm were not uniform in the TDP-leaf treatments. 3. Mortality rates during the late larval, cocoon spinning, and pupal stages were highest for the 30-50m leaf, especially mortality rates during the late larval and pupal stage were serious. 4. Pupation rate was lowest for the 30-50m leaf and those for the 300-400m and the 700-800m leaf were not significantly different from that of the control. 5. Nicotine damage to cocoon weight and cocoon shell weight was significant in each TDP-leaf level. Cocoon shell ratio was reduced at the same extent in each level, compared with the control. 6. The ratio among cocoon-classes was significantly different between treatments, compared with best-cocoon ratio of 87.1% for the control. Cocoons were not uniform for the 30-50m leaf, and those for the 300-400m and the 700-800m leaf were as almost uniform as those for the control. 7. Loss of fresh cocoon yield became greater as the distance of the mulberry-field from the TDP-got shorter. In conclusion, the critical distance of mulberry-field, which influences larval health, cocoon quality and yield, appeared to be 800m from the TDP. Such other factors as wind direction and topographic location may be involved in the critical distance. 8. From the present experiment, we could obtain only the effect of nicotine on the silkworm through digestive system, since the silkworm was raised at SES in Suweon. If the silkworm.. were raised in the vicinity of the TDP, poison effect of nicotine on the silkworm could beo expected through exoskeleton and tracheal system as well as through digestive system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.2
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• pp.198-203
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• 1989

Field experiment was conducted to find out the effect of lime materials application on yield, and chemical composition of Burley 21 in 1986. Lime materials and application rate were CaSO$_4$ : Ca 35kg/l0a. CaCO$_3$ : Ca 35kg/l0a and Liming: pH6.5. Contents of Ca and Mg in fresh or cured leaves were increased, but AI, Fe and Mn were decreased by applying lime materials. Yield were increased by applying lime materials. Among lime materials treatment, yield of limed and CaCO$_3$ plot were higher than that of CaSO$_4$ plot. Value per kg of cured leaves was not affected by applying lime materials. Cured leaves of CaSO$_4$ plot contained higher NH$_3$-nitrogen and alkalinity number of water insoluble ash than those of unlimed plot. Cured leaves of CaCO$_3$, plot contained higher alkalinity number of water soluble ash, insoluble ash and volatile neutral constituent, but lower protein-nitrogen and petroleum ether extracts than those of unlimed plot. Cured leaves of limed plot contained higher alkalinity number of water soluble and insoluble ash, NO$_3$-nitrogen and volatile neutral constituents, but lower protein -nitrogen, nicotine and petroleum ether extracts than those unlimed plot. Yield was increased, however leaf Quality in respect to chemical and organoleptic characteristics were not affected considerably by applying lime materials. Therefore, it suggests that controlling the soil pH about 6.5 by liming might be necessary for tobacco cultivation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.2
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• pp.252-257
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• 1983

This study was conducted to find out the stalk curing methods that can reduce photobleaching, and shorten the curing period in burley to bacco stalk curing. The results obtained summarized as follows. In the ventilation window-plot, the temperature within the curing house was high and the curing period was shortened, too. The rate of photobleached leaves of whole plant stalk curing-plot was higher than those of any other plots. In the ventilation window-plot, the labor of operating the curing house was saved and the yield and visual quality of cured leaves were high, too. For reducing the photobleaching of leaves, following procedures are desirable; to prevent the lower leaves from drying up and overripening at maturing stage, cut the stalk after first or second priming, shorten the curing period as possible when the 80% of leaf bodies changed brown.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.1
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• pp.45-52
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• 1989

The effects of soil properties and N, P and K application rates on the contents of inorganic constituents in the rhizomas of Ji-whang were investigated to know the condition for producing the better quality rhizoma of Ji-whang. Most of soil texture for Ji-whang cultivating belongs to sandy clay loam and sandy loam soils. The nitrogen contents in the rhizomas of Ji-whang was negatively correlated with the contents of organic matter and total nitrogen in surface soils, whereas positively correlated with the sand contents in subsoils. The calcium contents in the rhizomas of Ji-whang were negatively correlated with the sand contents, however, positively correlated with the contents of clay and organic matter in soils. The iron contents was positively correlated with the contents of clay, available phosphorus and exchangeable potassium in surface soils. Much more contents of the inorganic constituents in the leaf of Ji-whang was generally detected than that in the rhizoma of Ji-whang. The contents of iron and copper in the leaf was determined to be more than that in the rhizomas by ten times. In the results of field experiments, it is apparent that the nitrogen contents in the rhizoma and leaf increased with increasing N application rate more than 20kg/10a and 10kg/10a, respectively.