Background: Postoperative atrial fibrillation is the most frequently arrhythmic complication associated with coronary artery bypass graft surgery. This study was designed to investigate the incidence of atrial fibrillation in patients undergoing OPCAB and on-pump CABG and to identify the risk factors associated with its development. Material and Method: 247 consecutive patients were evaluated among 306 patients who underwent the coronary artery bypass graft surgery between January, 2002 and December, 2005. 178 patients underwent OPCAB (OPCAB group) and 69 patients underwent On-pump CABG (On-pump CABG group). The incidence and the risk factors of atrial fibrillation in two groups were determined. Result: There were no significant differences between two groups with respect to the preoperative demographic characteristics of the patients. The incidences of postoperative atrial fibrillation were 25 cases (14%) in OPCAB group and 15 cases (21%) in On-pump CABG group. Age over 65 years, net positive fluid imbalance for postoperative 3 days, and chest tube bleeding for postoperative 3 days were independent predictive factors in OPCAB group. Age over 65 years and net positive fluid imbalance for postoperative 3 days were independent predictive factors in On-pump CABG group. In multivariate analysis, age over 65 years was the only risk factor of postoperative atrial fibrillation in both groups. Conclusion: Atrial fibrillation is a common complication after procedures of myocardial revascularization. There wasn't a low incidence of postoperative atrial fibrillation in OPCAB, compared with On-pump CABG. Age over 65 years was associated with postoperative atrial fibrillation irrespective of the use of cardiopulmonary bypass.
Puroose: We examined whether intratumoral (i.t.) administration of dendritic cells (DCs) into a treated tumor could induce local and systemic antitumor effects in a mouse tumor model. Methods and Materials: C57BL/6 mice were inoculated s.c. in the right and left thighs with MCA-102 fibrosarcoma cells on day 0 and on day 7, respectively. On day 7, the tumors (usually 6 mm in diameter) on the right thigh were heated by immersing the tumor-bearing leg in a circulating water bath at $43^{\circ}C$ for 30 min; thereafter, the immature DCs were i.t administered to the right thigh tumors. This immunization procedure was repeated on days 7, 14 and 21. The tumors in both the right and left thighs were measured every 7 days and the average sizes were determined by applying the following formula, tumor $size=0.5{\times}(length+width)$. Cytotoxicity assay was done to determine tumor-specific cytotoxic T-lymphocyte activity. Results: Hyperthermia induced apoptosis and heat shock proteins (HSPs) in tumor occurred maximally after 6 hr. For the local treated tumor, hyperthermia (HT) alone inhibited tumor growth compared with the untreated tumors (p<0.05), and furthermore, the i.t. administered DCs combined with hyperthermia (HT + DCs) additively inhibited tumor growth compared with HT alone (p<0.05). On the distant untreated tumor, HT alone significantly inhibited tumor growth (p<0.05), and also HT + DCs potently inhibited tumor growth (p<0.001); however, compared with HT alone, the difference was not statistically significant. In addition, HT + DCs induced strong cytotoxicity of the splenocytes against tumor cells compared to DCs or HT alone. Conclusion: HT + DCs induced apoptosis and increased the expression of HSPs, and so this induced a potent local and systemic antitumor response in tumor-bearing mice. This regimen may be beneficial for the treatment of human cancers.
The study was conducted to find out synergistic effects by interaction on the basis on anatomical changes between paddy rice and weed species by mixture use of oxyfluorfen and bensulfuron at 0, $10^{-6}$ and $10^{-4}$M, respectively. Tissues were sampled at 72 hrs after soaking treatment and prepared for light microscopic examination and sectioned longitudinally and transversely stem into $8{\mu}m$ thick. As the results of microscopic examination, the major response of treated plant involved rupture and constrict in mesophyll cells and epidermal cells, and shrinks in bundle sheath cells by oxyfluorfen and disorganization and swelling in meristems by bensulfuron. Anatomical changes in rice was the least affected by even high concentration and two herbicide mixtures, whereas Echinochloa crus-galli was severely disorganized in meristem regions and ruptured in epidermal cells by mixture use. Monochoria vaginalis was completely ruptured by any treatment concentrations and mixtures. Scirpus juncoides was considerably tolerant to both herbicides and their mixture and was similar to those that occur in rice. Perennial weed, Sagittaria pigmaea was observed vacuolation, non-nucleation of cell, and irregular cell layering but in mixture injury was slight. Cyperus serotinus was severely shrunk and ruptured by mixture treatment. Eventually anatomical variations in all weed species was shown synergistic effect by use of herbicide mixtures.
Kim, Su Nam;Won, Chong Bock;Cho, Hye Jung;Eun, Byung Wook;Sim, So Yeon;Choi, Deok Young;Sun, Yong Han;Cho, Kang Ho;Son, Dong Woo;Tchah, Hann;Jeon, In Sang
Pediatric Infection and Vaccine
/
v.18
no.2
/
pp.135-142
/
2011
Purpose : Hospital associated infection (HAI) caused by multidrug-resistant (MDR) microorganisms has been recognized as an important issue in the world, especially in critically ill patients such as the patients admitted in the intensive care unit. There are fewer papers about MDR-HAI in pediatric patients compared to adult patients. In this study, we investigated the incidence and associated factors of MDR-HAI in children admitted to the intensive care unit (ICU) of a university hospital. Methods : We retrospectively evaluated 135 children who were admitted in ICU for at least 3 days between January 2009 and December 2010. HAI cases were divided into MDR-HAI group and non-MDR-HAI group. Clinical characteristics and various associated factors were compared between those groups. Results : In 39 patients, 45 cases of ICU-related HAI were developed. ICU-related HAI incidence was 47.7 per 1000 patientdays. Thirty-six cases (80.0%) were MDR-HAI. Acinetobacter baumannii was isolated more commonly in MDR-HAI group. And the followings were found more frequently in MDR-HAI group than non-MDR-HAI group: medical condition as an indication for ICU admission, mechanical ventilation, urinary catheterization and previous use of broad-spectrum antibiotics. Among the risk factors, previous use of broad-spectrum antibiotics was the independent risk factor for MDR-HAI. Conclusion : ICU-related HAI incidence was higher than previously reported. Previous use of broad-spectrum antibiotics was the independent risk factor for MDR-HAI. To investigate the characteristics of MDR-HAI in children admitted in ICU, further studies with a larger sample size over a longer period of time are warranted.
Osmotic and solid matrix priming treatments enhanced germination performance. We compared osmotic with solid matrix priming to determine the more effective treatment for improving seed germination in pepper and tomato. Seed hydration was immediately observed after osmotic priming and solid matrix priming treatment. The moisture content of solid matrix primed seeds was lower than that of osmotic primed seeds in the two vegetable crops. Osmotic priming and solid matrix priming did not increased percent germination, but showed shorter number of days to 50% of the final germination percentage ($T_{50}$) compared with untreated seeds, regardless of germination temperature. $T_{50}$ value was reduced in osmotic or solid matrix primed pepper seeds about 6.0, 5.0, 4.6 and 4.0 days compared with untreated seeds at 15, 20, 25, and $30^{\circ}C$, respectively. While, that in tomato seeds was reduced about 3.3, 5.0, 4.6 and 4.0 days compared with untreated seeds at 15, 20, 25, and $30^{\circ}C$, respectively. The effectiveness of osmotic priming or solid matrix priming in reducing the $T_{50}$ was greater when the seeds were germinated at $15^{\circ}C$ than at temperature of higher than $20^{\circ}C$. Solid matrix primed seeds germinated faster than osmotic primed seeds at all temperature in pepper. However, there was no significant difference on the percentage germination between solid matrix and osmotic primed seeds in tomato. After priming, dried-bark seeds showed faster germination than surface-dried seeds in pepper. On the other hand, there was no significant difference in tomato. Emergence of pepper and tomato seeds was markedly enhanced by osmotic priming or SMP treatment although the final emergence percentage was not significantly influenced. On the other hand, early growth was not significantly influenced by osmotic priming or SMP treatment of pepper and tomato seeds.
This experiment was conducted to investigate effects of non-ionic or zwitterionic (+/-) surfactants on digestibility of rice straw, and changes of growth of rumen mixed microbes, pH, and gas production during in vitro fermentation. Also, during in vitro ruminal fermentation, microbial attachment on rice straw was investigated using scanning electron microscopy (SEM). Tween 80 or SOLFA-850 for non-ionic surfactant (NIS), and 3-(Dodecyldimethylammonio) propanesulfanate (DDAP) for zwitterionic surfactant (ZIS) was supplemented by 0.05% and 0.1% in Dehority's artificial medium containing Holtein rumen fluid, respectively, and the substrate for fermentation was rice straw passed through 1 mm screen. The experiment was composed of 7 treatments (two levels of two NISs, two levels of a ZIS) including the control, and 6, 12, 24, 48 and 72 hr of fermentation time with 3 replications per treatment. Treatment of Tween 80 increased in vitro DM digestibilities during 48 hr and 72 h post fermentations compared to the other treatments, whereas treatment of DDAP as a ZIS resulted in decreased DM digestibility than that of the control from 24 hr post fermentation (P<0.05). Gas production in vitro was greater (P<0.05) with addition of NIS than the control or ZIS, and increased as fermentation time elapsed. Rumen mixed microbial growth was greatest with addition of Tween 80 as NIS, and lowest when DDAP as ZIS was supplemented to the fermentation tube (P<0.05). In SEM observation, rumen microbial population attached on rice straw particle was greater with addition of NIS, but was less with addition ZIS compared with the control. In conclusion we could not found any positive effects of ZIS surfactants on rumunal fermentation characteristics and rumen microbial growth rates.
Purpose: Endothelial progenitor cells (EPCs), which mediates neovascularization of uterine endometrium may be involved in the neovascularization in the utero-placental circulation. Low numbers of endothelial progenitor colony-forming unit (CFU) in culture are predictive biomarker of vascular disease. The aim of the present study was to evaluate whether the number of CFU in preeclampsia differed from that in normal pregnancy. Materials and Methods: Women with singleton normal (n=26) or preeclamptic (n=20) pregnancies were studied during the third trimester. The number of EPCs was quantified by CFU methodology. Plasma levels of angiogenic factors, vascular endothelial growth factor (VEGF), soluble fms-like tyrosine kinase-1 (sFlt-1), and placental growth factor (PlGF) were determined by enzyme-linked immunoassay. Results: CFU numbers were significantly decreased in the preeclamptic patients compared with the controls (median, 3; range 1-12 vs. 31; 3-81 CFU/well, P<0.001). A majority of the cells comprising individual colonies were positive for endothelial characteristics (Ulex europaeus lectin staining and acetylated low-density lipoprotein uptake). Plasma levels of the sFlt-1 were highly elevated (P<0.001) in patient with preeclampsia compared to controls, whereas PlGF were highly reduced (P=0.004), but these factors did not associate with CFU numbers. Conclusion: Our results suggest that reduced numbers of CFU obtained from maternal peripheral blood may contribute to the development of preeclampsia.
Park, Jun-Hyuk;Yoon, Ki-Yong;Park, Sang-Soo;Noh, Jin-Hwan;Lee, Ju-Sam
Korean Journal of Organic Agriculture
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v.19
no.3
/
pp.373-384
/
2011
This experiment was conducted to investigate the selection of regional double cropping system for production of organic forage in middle part of Korea and investigated their productivity, feed values and stock carrying capacity. The test results proved the Sorghum${\times}$Sudangrass hybrid was 10.9 $ton{\cdot}ha^{-1}$, the one of Rye + Red clover and Rye + Hairy vetch were the highest one as 3.3 $ton{\cdot}ha^{-1}$ and the relative yield about a Rye is higher in the dry matter yield in the field soil. And as for the relative forage values (RFV), Corn is the most excellent ones as 96.6 but there were no the significant difference between the Sorghum${\times}$Sudangrass hybrid of 84.4 and it. The total digestible nutrient (TDN) content in Rye + Hairy vetch is higher than others as 59.7%. As for the relative feed value, Rye + Red clover is the highest one as 83.8 but there was no significant differences between them. As for the ability to raise livestock in the field soil, the average values of $K_{CP}$ and $K_{TDN}$ are shown as 4.27 head/ha/yr in Sorghum${\times}$Sudangrass hybrid. As for the same crops, the average values of $K_{CP}$ and $K_{TDN}$ showed the highest ones as 1.74 head/ha/yr, in Rye + Red clover but there were no the significant differences. As for the dry matter yield of the rice field soil, organic rice straw was 3.3 $ton{\cdot}ha^{-1}$ and Rye + Red clover significantly showed the highest values as 4.1 $ton{\cdot}ha^{-1}$. In the rice filed soil, the crude protein content of organic rice straw was 3.1% and the TDN content of organic rice straw was 55%. The TDN content in Rye + Red clover was higher than others as 59.7%. As for the relative forage values, Rye + Red clover showed the highest one as 83.8 but there were no significant differences between them. As for the ability to raise livestock in the rice field, the average values of $K_{CP}$ and $K_{TDN}$ in the organic rice straw was 1.04 head/ha/yr and the average values of $K_{CP}$ and $K_{TDN}$ in Rye + Red clover is the highest one as 1.84 head/ha/yr, but there were no significant differences in all of them.
Yang, Seung Hak;Kim, Hyeon Shup;Cho, Won Mo;Kim, Sang Bum;Cho, Sung Back;Park, Kyu Hyun;Choi, Dong Yoon;Hwang, Sung Gu;Yoo, Yong Hee
Journal of Animal Environmental Science
/
v.18
no.sup
/
pp.47-54
/
2012
Effect of commercial Direct Fed Microbials (DFM) or protease treated feed (PTF) supplementation on growth rate and biogenic substances such as BUN, glucose, IgG, GOT, GPT and Vitamin A, C, E from Holstein steers was studied for 7 months. Thirty two steers aged 2~3 months were separated with 4 groups for control, DFM (PS), protease (ES) and their mix (PS + ES) supplementally fed 0, 100, 100 and 50 + 50 g/day respectively. Weight gain was averagely higher in PS than any others, although there were no differences significantly. All treatments enhanced to 3~8% of control in dry matter, crude protein and total digestible nutrient (P>0.05). Metabolic diseases with veterinary cure had not shown in this study. Plasma GOT and GPT were lower in the PS and ES than control. Plasma glucose concentration was also lower in PS than the others. Total cholesterol of ES was higher than the others but that of PS is the lowest. Plasma vitamin C was higher in PS than the others. It was shown that dietary PS affected change from glucose to vitamin C with not overloading liver. Conclusionally, PS and ES were shown to enhance metabolic health of steers during growing period.
Journal of The Korean Society of Grassland and Forage Science
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v.31
no.3
/
pp.295-304
/
2011
This study was carried out to investigate the effect of harvest stage of Sorghum ${\times}$ Sorghum Hybrid (SSH) and manufacture methods of SSH silage on nutritive values and quality of SSH silage manufactured with SSH grown in paddy land of Department of Animal Resources Development, National Institute of Animal Science, RDA. SSH "SS405" was harvested at two different growth stages (heading and ripen stage) and ensiled at each harvest stages. The content of crude protein in both square baled SSH silage (SBSS) and bag silage (BS) increased with delayed harvest maturity, but the contents of ADF (acid detergent fiber), NDF (neutral detergent fiber) decreased. The contents of ADF and NDF was not influenced by the inoculation of lactic bacteria. The contents of TDN (total digestible nutrient) in both stage and in vitro dry matter digestibility (IVDMD) in heading stage was not influenced by the harvest stage of SSH. The pH in all SSH silage ranged from 3.8 to 4.4 at two different harvest stages, and pH in heading stage was higher than that of ripen stage (P<0.05). The content of lactic acid of all SSH silage increased with delayed harvest maturity (P<0.05), but the content of acetic acid decreased (P<0.05). The contents of lactic acid and acetic acid in ripen stage were not influenced by manufacture method of silage. The content of lactic acid in both SBSS was higher than that of both BS and RBSS in heading and ripen stage (P<0.05), but the content of acetic acid decreased (P<0.05). The contents of lactic acid and acetic acid in BS were similar as compared to that of RBSS. Therefore, we suggest that the quality of SSH silage in both heading and ripen stage can be improved by manufacture methods of SBSS and BS.
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