Browse > Article
http://dx.doi.org/10.5352/JLS.2008.18.4.515

Effects of Non-ionic or Zwitterionic Surfactant on in vitro Digestibility of Rice Straw and Growth of Rumen Mixed Microorganisms.  

Lee, Shin-Ja (Division of Applied Life Science, Gyeongsang National University)
Kim, Wan-Young (Department of Animal Science, Korea National Agricultural College)
Moon, Yea-Hwang (Department of Animal Science & Biotechnology, Jinju National University)
Kim, Hyeon-Shup (National Livestock Research Institute, R.D.A.)
Kim, Kyoung-Hoon (Rural Development Administration)
Ha, Jong-Kyu (School of Agricultural Biotechnology, Seoul National University)
Lee, Sung-Sill (Division of Applied Life Science, Gyeongsang National University)
Publication Information
Journal of Life Science / v.18, no.4, 2008 , pp. 515-521 More about this Journal
Abstract
This experiment was conducted to investigate effects of non-ionic or zwitterionic (+/-) surfactants on digestibility of rice straw, and changes of growth of rumen mixed microbes, pH, and gas production during in vitro fermentation. Also, during in vitro ruminal fermentation, microbial attachment on rice straw was investigated using scanning electron microscopy (SEM). Tween 80 or SOLFA-850 for non-ionic surfactant (NIS), and 3-(Dodecyldimethylammonio) propanesulfanate (DDAP) for zwitterionic surfactant (ZIS) was supplemented by 0.05% and 0.1% in Dehority's artificial medium containing Holtein rumen fluid, respectively, and the substrate for fermentation was rice straw passed through 1 mm screen. The experiment was composed of 7 treatments (two levels of two NISs, two levels of a ZIS) including the control, and 6, 12, 24, 48 and 72 hr of fermentation time with 3 replications per treatment. Treatment of Tween 80 increased in vitro DM digestibilities during 48 hr and 72 h post fermentations compared to the other treatments, whereas treatment of DDAP as a ZIS resulted in decreased DM digestibility than that of the control from 24 hr post fermentation (P<0.05). Gas production in vitro was greater (P<0.05) with addition of NIS than the control or ZIS, and increased as fermentation time elapsed. Rumen mixed microbial growth was greatest with addition of Tween 80 as NIS, and lowest when DDAP as ZIS was supplemented to the fermentation tube (P<0.05). In SEM observation, rumen microbial population attached on rice straw particle was greater with addition of NIS, but was less with addition ZIS compared with the control. In conclusion we could not found any positive effects of ZIS surfactants on rumunal fermentation characteristics and rumen microbial growth rates.
Keywords
Non-ionic surfactant; zitterionic (+/-) surfactant; digestibility; SEM;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Munn, E. A., G. P. Hazlewood and M. Graham. 1983. Uptake and incorporation of the products of proteolysis by the rumen bacterium bacteroides ruminicola R8/4. Curr. Microbiol. 8, 317-320   DOI
2 Reese, E. T. and A. Maguire. 1969. Surfactants as stimulants of enzyme production by microorganisms. Applied Microbiol. 17, 242-245
3 SAS. 1999. SAS/STAT software for PC. Release 8.01. SAS institute Inc., Cary, N.C., U.S.A
4 Yazdi, M. T., J. R. Woodward and A. Radford. 1990. The cellulase complex of Neurospora crassa: activity, stability and release. J. Gen. Microbiol. 136, 1313-1319   DOI   ScienceOn
5 Goto, M., H. D. Bae, M. S. Yahaya, S. Karita, K. Wangjae, K. Sugawara and K. J. Cheng. 2003. Effect of surfactants Tween 80 on enzymatic accessibility and degradation of orchardgrass (Dactylis gromerata L.) at different growth stages, Asian-Aust. J. Anim. Sci. 16, 83-87   DOI
6 Lee, S. S. and J. K. Ha. 2003. Influences of surfactants Tween 80 on the gas production, cellulose digestion activities by mixed rumen microorganisms. Asian-Aust. J. Anim. Sci. 16, 1151-1157   DOI
7 Hulme, M. A. and D. W. Stranks, 1970. Induction and the regulation of production of cellulase by fungi. Nature London 226, 469-470   DOI   ScienceOn
8 Hung, B. R., L. Lara, M. A. Patron, N. N. Ugarova, W. Bechestedt and S. Clappes, 1988. Tween 80 and proteose peptone effect on cellulase production. Acta. Biotechnol. 8, 461-464   DOI
9 Kamande, G. M., J. Baah, J. K. Cheng, T. A. McAllister and J. A. Sheford. 2000. Effects of Tween 60 and Tween 80 on protease activity, thiol group reactivity, protein absorption, and cellulose degradation by rumen microbial enzymes. J. Dairy Sci. 83, 536-542   DOI   ScienceOn
10 Lang, S. and F. Wagner. 1993. Biological activities of biosurfactants. Kosaric Biosurfactants, Marcel Dekker, Inc. New York, USA
11 Lee, S. S., B. H. Ahn, H. S. Kim, C. H. Kim, K. J. Cheng and J. K. Ha. 2003. Effects of non-ionic surfactants on enzyme distributions of rumen contents, anaerobic growth of rumen microbes, rumen fermentation characteristics and performances of lactating cows. Asian-Aust. J. Anim. Sci. 16, 104-115   DOI
12 Lee, S. S., J. K. Ha, H. S. Kang and K. J. Cheng. 1997. Industrial application of anaerobic rumen fungi: A review. Korean J. Dairy Sci. 19, 249-276
13 Lee, S. S., K. J. Shin, W. Y. Kim, J. K. Ha and I. K. Han. 1999. The rumen ecosystems: As a fountain source of novel enzymes -Review article. Asian-Aust. J. Anim. Sci. 12, 988-1001   DOI   ScienceOn
14 Long, K. and J. S. Knapp. 1991. The effect of Junlon PW110 and Tween 80 on the production of cellulolytic enzymes by Coprinus cinereus. Mycol. Res. 95, 1077-1081   DOI
15 Moore, J. E. 1970. Procedures for the two-stage in vitro digestion of forages. pp 5001-5003, In Harris, L. E. (ed.), Nutrition research techniques for domestic and wild animals, Vol. 1. Utah State Univ., Logan, UT
16 A.O.A.C. 1995. Official methods of analysis 16th edition. Association of official analytical chemists, Washington, D.C.
17 Busquet, M., S. Calsamiglia, A. Ferret and C. Kamel, 2006. Plant extracts affect in vitro rumen microbial fermentation. J. Dairy Sci. 89, 761-771   DOI   ScienceOn
18 De Oude, N. T. 1992. The handbook of environmental chemistry. Vol. 3, part F: anthropogenic compounds. Springer Verlag, Heidelberg, FRG
19 Goto, M., H. D. Bea, S. S. Lee, M. S. Yahaya, S. Karita, K. Wangjae and K. J. Cheng. 2003. Effect of surfactants Tween 80on forage degradability and microbial growth on the in vitro rumen mixed and pure cultures. Asian-Aust. J. Anim. Sci. 16, 672-676   DOI
20 Ho, Y. W., N. Abdullah and S. Jalaludin. 1988. Penetrating structures of anaerobic rumen fungi in cattle and swamp buffalo. J. Gen. Micobial. 134 177-181
21 Dehority, B. A. and H. W. Scott. 1967. Extent of cellulose and hemicellulose digestion in various forage by pure cultures of rumen bacteria. J. Dairy Sci. 50, 1136-1141   DOI
22 Deshpande, M. V., M. C. Srinavasan and S. S. Deshmakh. 1987. Effects of fatty acids on cellylase production by Penicillium funiculosum and its mutants. Biotechnol. Lett. 9, 301-304   DOI
23 Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics. 11, 1-42   DOI   ScienceOn
24 Ferorak, P. M. and S. E. Hrwdey. 1983. A simple apparatus for measuring gas production by methanogenic cultures in serum bottles. Environ. Technol. Lett. 4, 425-432   DOI   ScienceOn
25 Yang, C. M. and J. B. Russell. 1993. The effect of monen supplementation on ruminal ammonia accumulation in vitro and the numbers of amino acid-fermenting bacteria. J. Anim. Sci. 71, 3470-3476   DOI
26 Lee, S. S., H. S. Kim, Y. H. Moon, N. J. Choi and J. K. Ha. 2004. The effects of a non-ionic surfactants on the fermentation characteristics, microbial growth, enzyme activity and digestibility in the rumen of cows. Anim. Feed Sci. and Technol. 115, 37-50   DOI   ScienceOn