• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2005년도 추계학술대회 학술발표 논문집 제15권 제2호
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• pp.535-541
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• 2005

It has been suggested that the endoscopic color of intramucosal gastric carcinoma is correlated with mucosal vascularity within the carcinomatous tissue. The development of electronic endoscopy has made it possible to quantitatively measure the mucosal hemoglobin volume, using a hemoglobin index. The aim of this study was to make a software program to calculate the hemoglobin index (IHb) and then investigate whether the mucosal IHb determined from the electronic endoscopic data is a useful marker for evaluating the color of intramucosal gastric carcinoma, in particular with regard to its value for discriminating between the histologic type. The mean values of IHb for the carcinoma (IHb-C) and the mean values of IHb for the surrounding non-cancerous mucosa ( IHb-N) were calculated in 75 intestinal-type and 34 diffuse-type gastric carcinomas. Then, we analyzed the ratio of the IHb-C to IHb-N. The mean IHb-C/IHb-N ratio in the intestinal-type carcinoma group was higher than that in the diffuse-type carcinoma group ($1.28{\pm}0.19$ vs. $0.81{\pm}0.18$, respectively, p<0.001). When the cut-off point of the C/N ratio was set at 1.00, the accuracy rate, the sensitivity, the specificity, and the positive and negative predictive values of a C/N ratio below 1.00 for the differential diagnosis of diffuse-type carcinoma from intestinal-type carcinoma were $94.5\%$, $94.1\%$, $94.7\%$, $88.9\%$ and $97.3\%$, respectively. IHb is useful for quantitative measurement of the endoscopic color in intramucosal gastric carcinoma and the IHb-C/IHb-N ratio would be helpful in distinguishing diffuse-type carcinoma from intestinal -type carcinoma.

• Journal of Genetic Medicine
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• 제2권1호
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• pp.41-47
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• 1998

Human uniparental gestations such as gynogenetic ovarian teratomas provide a model to evaluate the integrity of parent-specific gene expression - i.e. imprinting - in the absence of a complementary parental genetic contribution. The few imprinted genes characterized so far include the insulin-like growth factor-2 gene (IGF2) coding for a fetal growth factor and H19 gene whose normal function is unknown but it is likely to act as an mRNA. IGF2 is expressed by the paternal allele and H19 by the maternal allele. This reciprocal expression is quite interesting because both H19 and IGF2 genes are located close to each other on chromosome 11p15.5. In situ RNA hybridization analysis has shown variable expression of the H19 and IGF2 alleles according to the tissue origin in 11 teratomas. Especially, Skin, derivative of ectoderm, is expressed conspicuously. We examined imprinting of H19 and IGF2 in teratomas using PCR and RT-PCR of exonic polymorphism. H19 and IGF2 transcript could be expressed either biallelically or monoallelically in the teratomas. Biallelic expression (i.e., loss of imprinting) of IGF2 occurred in 5 out of 6 mature teratomas and 1 out of 1 immature teratoma. Biallelic expression of H19 occurred in 4 out of 10 mature teratomas and 1 out of 1 immature teratoma. Expression levels of H19 and IGF2 transcript using the semi-quantitative RT-PCR had no relation between monoallelic and biallelic expression. Moreover, IGF2 biallelic expression did not affect allele-specificity or levels of H19 expression. These results demonstrate that both genes, H19 and IGF2, can be imprinted, expressed and regulated independently and individually of each other in ovarian teratoma.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2004년도 제21차 정기총회 및 학술발표회
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• pp.16-18
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• 2004

본 연구는 닭의 여러 조직별 세포들의 telomere 함유율과 telomerase 활성도를 분석 제시하고자 하였다. 한국재래계의 수정란 및 발생단계별 신생 조직과 출생 후 성장단계별 각 조직들에 대한 telomere의 함량과 telomerase 활성도를 분석하였고, 초기배자, 간, 뇌, 신장, 심장, 생식선 조직 및 백혈구 세포를 분석대상으로 하였다. Telomere의 함량 분석은 chicken telomeric DNA probe를 이용한 Q-FISH법으로 수행하였고, telomerase activity의 분석은 TRAP법을 이용하였다. 분석결과 초기 배자, 생식선 세포 및 신장세포에서는 지속적으로 매우 높은 telomerase activity를 나타내었으나 뇌, 심장, 간 등에서는 발생 및 발육이 진행됨에 따라 유의적 감소 양상을 보였다. 닭의 각 조직별 telomere의 함량 분석결과, 대부분의 세포들이 성장이 진행됨에 따라 telomere 함유율이 감소되는 양상을 보였다. 이상의 결과로부터 telomerase의 활성도와 telomere의 함량간에 매우 밀접한 연관성을 보이며. 이들이 닭 조직별 세포의 분화 및 증식성 특이성과도 밀접한 관련이 있는 것으로 나타났다.

• 한국동물위생학회지
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• 제28권1호
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• pp.81-89
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• 2005

Recently, mass spectrometry coupled with liquid chromatography (LC/MS) has been a preferred technique for determination of organic compounds in complex matrixes. LC/MS provides a high degree sensitivity and specificity of the compounds of interest. The purpose of this study was to confirm analytical method of residual 6 benzimidazoles (thiabendazole, oxfendazole, mebendazole, albendazole, flubendazole and fenbendazole) in meat by LC/MS. Benzimidazoles were analyzed by LC/MS on XTerra $C_{18}$ column with 0.01% trifluoroacetic acid-acetonitrile (TFA) in a gradient mode as mobile phase, and that were identified by electrospray ionization with selected ion recording mode at 150-350 amu mass range. Residual benzimidazoles were extracted from tissue with ethylacetate, and elute benzimidazoles with $50\%$ acetonitrile. In the LC/MS analysis of benzimidazoles, signal to noise ratio was showed relatively high in the positive mode and special ion in the quality analysis was determined via $[M+H]^+$ and Fragment ions. A spectrum of benzimidazoles was showed from all 6 benzimidazoles

• 환경생물
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• 제24권2호
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• pp.155-159
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• 2006

To reveal effects of gamma-irradiation with various doses on the expressions of C4H and F5H genes, the transcription levels of OsC4HL and OsF5HL were investigated in leaves and stems of two rice cultivars, Ilpoombyeo and IR-29, after the irradiation with 5, 10, 50, or 100 Gy for 4 h. In overall pattern of 24 h after the irradiation, the transcription levels of the two genes increased with the increasing doses of radiation in the leaves of both cultivars, except that of OsC4HL in IR-29. However, in the stems, the transcription level of OsF5HL increased in Ilpoombyeo and decreased in IR-29 dose-dependently, while that of OsC4HL decreased in Ilpoombyeo with the increasing doses of radiation and remained constant in IR-29. When the expressions of OsC4HL and OsF5HL were investigated in a time-course after the irradiation with 100 Gy, they reached their highest levels in the leaves of both cultivars 5 hand 72 h after the irradiation, respectively. Therefore, we suggest that the expressions of OsC4HL and OsF5HL, which involved in the same phenylpropanoid pathway, are differentially regulated during the post-irradiation period, showing different cultivar and tissue specificity. Furthermore, the dose dependency of the gene expressions is also discussed immediately after the irradiation.

• Asian Pacific Journal of Cancer Prevention
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• 제15권21호
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• pp.9439-9444
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• 2014

Purpose: To identify prostate cancer lncRNAs using a pipeline proposed in this study, which is applicable for the identification of lncRNAs that are differentially expressed in prostate cancer tissues but have a negligible potential to encode proteins. Materials and Methods: We used two publicly available RNA-Seq datasets from normal prostate tissue and prostate cancer. Putative lncRNAs were predicted using the biological technology, then specific lncRNAs of prostate cancer were found by differential expression analysis and co-expression network was constructed by the weighted gene co-expression network analysis. Results: A total of 1,080 lncRNA transcripts were obtained in the RNA-Seq datasets. Three genes (PCA3, C20orf166-AS1 and RP11-267A15.1) showed a significant differential expression in the prostate cancer tissues, and were thus identified as prostate cancer specific lncRNAs. Brown and black modules had significant negative and positive correlations with prostate cancer, respectively. Conclusions: The pipeline proposed in this study is useful for the prediction of prostate cancer specific lncRNAs. Three genes (PCA3, C20orf166-AS1, and RP11-267A15.1) were identified to have a significant differential expression in prostate cancer tissues. However, there have been no published studies to demonstrate the specificity of RP11-267A15.1 in prostate cancer tissues. Thus, the results of this study can provide a new theoretic insight into the identification of prostate cancer specific genes.

• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.3003-3007
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• 2015

Objective: To explore the application of multiplex nested methylated specific polymerase chain reaction (PCR) in the early diagnosis of epithelial ovarian carcinoma (EOC). Materials and Methods: Serum and fresh tissue samples were collected from 114 EOC patients. RUNX3, TFPI2 and OPCML served as target genes. Methylation levels of tissues were assessed by multiplex nested methylated specific PCR, the results being compared with those for carcinoma antigen 125 (CA125). Results: The serum free deoxyribose nucleic acid (DNA) methylation spectrum of EOC patients was completely contained in the DNA spectrum of cancer tissues, providing an accurate reflection of tumor DNA methylation conditions. Serum levels of CA125 and free DNA methylation in the EOC group were evidently higher than those in benign lesion and control groups (p<0.05). Patients with early EOC had markedly lower serum CA125 than those with advanced EOC (p<0.05), but there was no significant difference in free DNA methylation (p>0.05). The sensitivity, specificity and positive predicative value (PPV) of multiplex nested methylated specific PCR were significantly higher for detection of all patients and those with early EOC than those for CA125 (p<0.05). In the detection of patients with advanced EOC, the PPV of CA125 detection was obviously lower than that of multiplex nested methylated specific PCR (p>0.05), but there was no significant difference in sensitivity (p>0.05). Conclusions: Serum free DNA methylation can be used as a biological marker for EOC and multiplex nested methylated specific PCR should be considered for early diagnosis since it can accurately determine tumor methylation conditions.

• Genomics & Informatics
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• 제11권3호
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• pp.142-148
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• 2013

SINE-VNTR-Alu (SVA) elements are present in hominoid primates and are divided into 6 subfamilies (SVA-A to SVA-F) and active in the human population. Using a bioinformatic tool, 22 SVA element-associated genes are identified in the human genome. In an analysis of genomic structure, SVA elements are detected in the 5′ untranslated region (UTR) of HGSNAT (SVA-B), MRGPRX3 (SVA-D), HYAL1 (SVA-F), TCHH (SVA-F), and ATXN2L (SVA-F) genes, while some elements are observed in the 3′UTR of SPICE1 (SVA-B), TDRKH (SVA-C), GOSR1 (SVA-D), BBS5 (SVA-D), NEK5 (SVA-D), ABHD2 (SVA-F), C1QTNF7 (SVA-F), ORC6L (SVA-F), TMEM69 (SVA-F), and CCDC137 (SVA-F) genes. They could contribute to exon extension or supplying poly A signals. LEPR (SVA-C), ALOX5 (SVA-D), PDS5B (SVA-D), and ABCA10 (SVA-F) genes also showed alternative transcripts by SVA exonization events. Dominant expression of HYAL1_SVA appeared in lung tissues, while HYAL1_noSVA showed ubiquitous expression in various human tissues. Expression of both transcripts (TDRKH_SVA and TDRKH_noSVA) of the TDRKH gene appeared to be ubiquitous. Taken together, these data suggest that SVA elements cause transcript isoforms that contribute to modulation of gene regulation in various human tissues.

• Parasites, Hosts and Diseases
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• 제32권4호
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• pp.231-242
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• 1994

자연 감염된 가재에서 폐흡충의 피낭유충을 분리하고 개에 경구 감염시켜 성충을 얻었다. 폐흡충 성충의 조효소를 ion-exchange chromatography, affinity chromatography와 gel filtration chromatoglaphy를 실시하여 cysteine proteinase를 순수 정제하였다. 이들 효소의 생화학적 특성과 분해능을 관찰하였으며. 효소면역전기영동이적법을 이용하여 순수 정제한 효소의 항원성을 관찰하였다. 정제된 효소는 저분자 합성기질인 CBZ-arg-arg-AFC 보다 CBZ-phe-arg-AFC에서 높은 활성을 보였으며. 이들 효소는 thiol-dependent이었다. 정제된 효소 및 조효소의 최적 pH는 5.5이었고. 최적 mole 농도는 0.1 M(0.1 M sodium citrate, pH 5.5)이었고, 이들 효소는 $4^{\circ}C$에서 48시간 동안 80%의 안정성을 보였다 정제된 효소의 native 분자량은 20.000 dalton이었고, SDS- PAGE상에 나타난 분자량은 17,500 dalton이었다 정제된 효소는 cysteine proteinase 특이 억제 인자인 E-64, lodoacetic acid, NEM에 의해 활성이 완전히 억제되었으며, serine proteinase, aspartic proteinase 및 metallo proteinase 특이 억제인자에 의해 활성이 억제되지 않았다. 정제된 효소는 collagen(Type I)과 hemoglobin을 분해하였고, 효소면역전기영동이적법으로 정제된 효소의 항원성을 확인하였다.

• 식물조직배양학회지
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• 제27권5호
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• pp.415-421
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• 2000

The disaccharide trehalose ($\alpha$-D-glucopyranosyl-$\alpha$-D-glucopyranoside) is found in variety of organ-isms that are able to withstand almost complete desiccation. In order to identify the function of trehalose in plants, we isolated Arabidopsis trehalase (AtTRE) gene that encodes the enzyme able to hydrolyze trehalose to glucose, and trehalose-6-phosphate synthase isolog, TPS3 gene by RT-PCR. The AtTRE had the substrate specificity to hydrolyze only trehalose, and a broad pH range of enzyme activity. The AtTRE promoter/GUS reporter gene was expressed in cotyledons, mature leaf tissues including guard cells, and developing siliques. The GUS expression driven by AtTPS3 promoter was significant in root tissues, and the level of GUS activity was much higher than that of the pBll 21 control seedlings. The knockout of AtTPS3 gene in Arabidopsis resulted in the retarded root development, whereas the overexpression of AtTPS3 increased the root elongation in the presence of sucrose in MS medium. Possible functions of AtTRE and AtTPS3 in plant will be discussed. In addition, ectopic expression of yeast TPS1 driven by the inducible promoters in tobacco and potato conferred the plants on the drought and freezing tolerances.