Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Study on analytical method of residual benzimidazole anthelmintics in meat by LC/MS

LC/MS를 이용한 식육중 잔류 벤지미다졸계 구충제 분석법 연구

  • Choi Eun-Young (Jeollabuk-do Livestock Development and Research Institute) ;
  • Seo Heyng-Seok (Jeollabuk-do Livestock Development and Research Institute) ;
  • Baek Kui-Jeong (Jeollabuk-do Livestock Development and Research Institute) ;
  • Hur Boo-Hong (Jeollabuk-do Livestock Development and Research Institute) ;
  • Seo Lee-Won (Jeollabuk-do Livestock Development and Research Institute) ;
  • Joung Dong-Suk (Jeollabuk-do Livestock Development and Research Institute)
  • 최은영 (전라북도 축산진흥연구소) ;
  • 서형석 (전라북도 축산진흥연구소) ;
  • 백귀정 (전라북도 축산진흥연구소) ;
  • 허부홍 (전라북도 축산진흥연구소) ;
  • 서이원 (전라북도 축산진흥연구소) ;
  • 정동석 (전라북도 축산진흥연구소)
  • Published : 2005.03.01
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Abstract

Recently, mass spectrometry coupled with liquid chromatography (LC/MS) has been a preferred technique for determination of organic compounds in complex matrixes. LC/MS provides a high degree sensitivity and specificity of the compounds of interest. The purpose of this study was to confirm analytical method of residual 6 benzimidazoles (thiabendazole, oxfendazole, mebendazole, albendazole, flubendazole and fenbendazole) in meat by LC/MS. Benzimidazoles were analyzed by LC/MS on XTerra $C_{18}$ column with 0.01% trifluoroacetic acid-acetonitrile (TFA) in a gradient mode as mobile phase, and that were identified by electrospray ionization with selected ion recording mode at 150-350 amu mass range. Residual benzimidazoles were extracted from tissue with ethylacetate, and elute benzimidazoles with $50\%$ acetonitrile. In the LC/MS analysis of benzimidazoles, signal to noise ratio was showed relatively high in the positive mode and special ion in the quality analysis was determined via $[M+H]^+$ and Fragment ions. A spectrum of benzimidazoles was showed from all 6 benzimidazoles

Keywords

References

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