• Title/Summary/Keyword: Tissue of bamboo

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Tissue-cultured regeneration and ecological values in major bamboo species

  • Sharma, Avinash;Manpoong, Chowlani;Gohain, Anwesha;Pandey, Himanshu;Padu, Gompi;Aku, Hage
    • Journal of Ecology and Environment
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    • v.46 no.3
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    • pp.218-242
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    • 2022
  • Background: Promising specific growth regulators are employed in the tissue cultures of various bamboo species. Specific natural hardening mixtures support the acclimatization and adaptation of bamboo under protected cultivation. Results: The growth regulators like 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Naphthaleneacetic Acid (NAA), Thidiazuron (TDZ), 6-Benzylaminopurine (BAP), Kinetin, Gelrite, Benzyl Adenine (BA), Indole Butyric Acid (IBA), Coumarin, Putrescine, Gibberellic acid (GA3), Indole Acetic Acid (IAA) has been widely used for callus induction, root regeneration and imposing plant regeneration in various species of bamboo such as Bambusa spp. and Dendrocalamus spp. Different combinations of growth regulators and phytohormones have been used for regenerating some of the major bamboo species. Natural hardening materials such as cocopeat, vermicompost, perlite, cow dung, farmyard manure, compost, soil, garden soil, and humus soil have been recommended for the acclimatization and adaptation of bamboo species. Standard combinations of growth regulators and hardening mixtures have imposed tissue culture, acclimatization, and adaptation in major bamboo species. Conclusions: Bamboo contributes to soil fertility improvement and stabilization of the environment. Bamboo species are also involved in managing the biogeochemical cycle and have immense potential for carbon sequestration and human use. This paper aims to review the various growth regulators, natural mixtures, and defined media involved in regenerating major bamboo species through in vitro propagation. In addition, the ecological benefits of safeguarding the environment are also briefly discussed.

Microscopic Observation of Moso bamboo(Phyllostachys pubescens Mazel) with Various Ages (맹종죽의 죽령별 조직 현미경적 관찰)

  • Yoon, Seung-Lak
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.42 no.2
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    • pp.27-34
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    • 2010
  • To obtain the basic information for the use of bamboo, the anatomical characteristics of moso bamboo shoots, and 20-day-old, 60-day-old, one-year-old, and two-year-old moso bamboo were observed by using polarizing microscope. The cross section showed that the bamboo shoots consists of the early stage of cell formation, atactostele, parenchyma, and bundle sheath was formed in 20-day-old moso bamboo, and all cells completely formed in 60-day-old moso bamboo. The tissues in one-year-old moso bamboo appeared to be completely matured. On the tangential surface, no atactostele was observed in the bamboo shoot, but metaxylem, parenchyma, and bundle sheath were found. atactostele and parenchyma appeared to be formed between 20 and 60 days. All tissues and cells were totally formed and the cell wall of parenchyma were completely matured after one year. The metaxylem in bamboo shoots was observed to have pits, which means that it could be the tissues first formed of all bamboo cells.

A Study on the Utilization of Ingredients and Fibers from Korean Bamboo Species in Value-added Industry: Part 2. Preparation and Characterization of Bamboo Fibers (국내산 죽종의 화학성분 및 섬유소 이용에 관한 연구 (제2보) - 대나무 섬유의 제조 및 특성 -)

  • Kang, Kyu-Young;Yoon, Seung-Lak;Jeon, Kwon-Seok
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.44 no.4
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    • pp.69-76
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    • 2012
  • The bamboo fibers from three Korean bamboo species, Moso, Henon and Timber bamboo, were prepared by disintegration after thermal treatment. The samples were prepared according to the age of growth; bamboo shoots, 20 days and 50 days of growth. The fiber quality was also investigated by morphological and chemical analyses. There was no big difference in the dry mass of fibers among the three bamboo species. However, the dry mass of fibers from 50 days of growth was increased by 34%, whereas it was ranged in 9-20% in the case of fibers from bamboo shoots and 20 days of growth. In the results of morphological analysis, the fibers could be classified as the fibers from metaxylem, the fibers from parenchyma and the fines produced during disintegration. The fibers from 50 days of growth were separated from metaxylem, whereas the fibers from bamboo shoots and 20 days of growth were mainly consisted of fibers from metaxylem and parenchyma. The chemical analysis of fibers showed that the contents of carbohydrates, lignin and extractives were not much affected by thermal treatment.

Bamboo salt attenuates $CCl_4$-induced hepatic damage in Sprague-Dawley rats

  • Zhao, Xin;Song, Jia-Le;Kil, Jeung-Ha;Park, Kun-Young
    • Nutrition Research and Practice
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    • v.7 no.4
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    • pp.273-280
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    • 2013
  • Bamboo salt, a Korean folk medicine, is prepared with solar salt (sea salt) and baked several times at high temperatures in a bamboo case. In this study, we compared the preventive effects of bamboo salt and purified and solar salts on hepatic damage induced by carbon tetrachloride in Sprague-Dawley rats. Compared with purified and solar salts, bamboo salts prevented hepatic damage in rats, as evidenced by significantly reduced serum levels of aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase (P < 0.05). Bamboo salt (baked $9{\times}$) triggered the greatest reduction in these enzyme levels. In addition, it also reduced the levels of the proinflammatory cytokines interleukin (IL)-6, interferon (IFN)-${\gamma}$, and tumor necrosis factor (TNF)-${\alpha}$. Histopathological sections of liver tissue demonstrated the protective effect of bamboo salt, whereas sections from animals treated with the other salt groups showed a greater degree of necrosis. We also performed reverse transcription-polymerase chain reaction and western blot analyses of the inflammation-related genes iNOS, COX-2, TNF-${\alpha}$, and IL-$1{\beta}$ in rat liver tissues. Bamboo salt induced a significant decrease (~80%) in mRNA and protein expression levels of COX-2, iNOS, TNF-${\alpha}$, and IL-$1{\beta}$, compared with the other salts. Thus, we found that baked bamboo salt preparations could prevent $CCl_4$-induced hepatic damage in vivo.

STUDIES ON THE OYSTER DISEASES 1. Pathogenetic Investigation (굴의 질병에 관한 연구 1, 병리 조직학적 연구)

  • CHUN Seh Kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.3 no.1
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    • pp.7-18
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    • 1970
  • The present paper deals with mortality and pathogenetic investigation of the oysters Crassostrea gigas cultured by tile coventional bamboo and hanging method in Kimhae and Koje-Do in 1969. The results of the investigation may be summarized as follows: 1. Mortality of the oysters by the bamboo method in Kimhae was $9.5\%$ in June, $10.2\%$ In July, $12.3\%$ in August and $12.1\%$ in September, respectively, 2. Mortality of the oysters cultured by the hanging method in Koje-Do was $2.4\%$ in June, $2.9\%$ in July, $30.7\%$ in October, $37.3\%$ in November and $30.0\%$ in December, respectively 3. The diseased oysters had severe inflammation, necrosis and multiple abscess in the epithelia of stomach, mid-gut, digestive tubules, blood vessels and gonads, mucous membrane and surrounding tissue. 4. From August gram negative bacteria were found in the nodules of connective tissue and multiple abscess of the diseased oysters. Particularly the connective tissue of the diseased oysters contained more bacteria than epithelia. 5. Since the bacteria are less abundant in the region of digenerated tissue, mortality of the oysters is not caused only by the infectious bacteria but seems that is also caused by other environmental factors such as extreme temperatures and salinities.

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Effect of Vascular Bundles and Fiber Sheaths in Nodes and Internodes of Gigantochloa apus Bamboo Strips on Tensile Strength

  • Atmawi DARWIS;Anne HADIYANE;Endah SULISTYAWATI;Ihak SUMARDI
    • Journal of the Korean Wood Science and Technology
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    • v.51 no.4
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    • pp.309-319
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    • 2023
  • Bamboo culm is in the form of a tube/pipe, composed of internodes which are bounded by a partition/diaphragm (node). Anatomically, bamboo is composed of vascular bundles and parenchyma ground tissue. One of the constituents of vascular bundles is fibers that are grouped to form a fiber sheath. The anatomical structure of the nodes and internodes is thought to influence the strength of bamboo strips, including tensile strength. This study aimed to determine the characteristics of vascular bundles (distribution and fiber percentage) and their effects on the density and tensile strength of Gigantochloa apus bamboo strips with and without nodes. The bamboo culms were divided into three parts (outer, middle, and inner) along the radial direction. The results showed that the distribution of vascular bundles and percentage of fiber sheaths decreased significantly from the outer to the inner layer. This also had a significantly decreased density and tensile strength. Furthermore, the number of vascular bundles (in the transverse plane) was greater in the internodes than in the nodes. Anatomically, the orientation of the vascular bundles at irregular nodes is observed in the radial and tangential planes, where the direction is not only in the axial direction, but also in the radial and tangential directions. This caused the tensile strength of the G. apus bamboo strips to be lower at the nodes than at the internodes.

Synergistic combination effect of anti-obesity in the extracts of Phyllostachys pubescence Mael and Scutellaria baicalensis Georgi (죽엽(竹葉)과 황금(黃芩) 복합물의 항비만 효과)

  • Kang, Young Min;Kim, Seung-Hyung;Lee, Young-Cheol;Kim, Ho Kyoung;Kim, Dong-Seon
    • The Korea Journal of Herbology
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    • v.29 no.6
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    • pp.7-13
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    • 2014
  • Objectives : Anti-obesity drugs that have been developed so far have limited efficacies and considerable adverse effects affecting tolerability and safety. Therefore, most anti-obesity durgs have been withdrawn. We tried to develop anti-obesity agent by combinations from herbs that are used in food ingredients as well as in traditional medicines. Methods : The 80% (v/v) ethanol extracts from Bamboo (Phyllostachys pubescence) leaf (BL) and Scutellaria baicalensis (SB) and their 1:1 combination (BLSB) was evaluated on high fat diet induced obese mice compared to Omega-3 as a positive control. The mice were divided into six groups (n=5), one group fed a normal diet (ND), and the others fed a high fat diets for eight weeks. Two weeks after starting feeding the diets, the high fat diet groups were orally administered vehicle and Omega-3, BL, SB, and BLSB at dosage of 200 mg/kg/day for six weeks. All groups were assayed for body weights, food efficiency ratio, blood biochemistry parameters, and organic tissue weights. Results : BLSB group showed significant reductions in body weight gain and fat weights of liver and epididymal adipose tissue compared to BL or SB alone as well as control. Total-cholesterol and LDL-cholesterol levels significantly decreased, and HDL-cholesterol level increased. In liver tissue, macrovesicular steaotisis was remarkably improved and its fat cell size was also significantly decreased. Conclusions : These results suggested that a combination preparation of bamboo leaf and S. baicalensis has anti-obesity effect and have synergistic effect compared to bamboo leaf or S. baicalesis.

Morphological Characteristics of Bambusa vulgaris and the Distribution and Shape of Vascular Bundles therein

  • Darwis, Atmawi;Iswanto, Apri Heri
    • Journal of the Korean Wood Science and Technology
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    • v.46 no.4
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    • pp.315-322
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    • 2018
  • Bamboo culm comprises internodes and nodes and is tapered from the bottom to the top. Anatomically, bamboo culm comprises vascular bundles and parenchymal base tissue. The gross anatomical structure of a transverse section of any culm internode is determined by the shape, size, arrangement, and number of vascular bundles. The purpose of this research was to examine the morphology of culm and the distribution and shape of vascular bundles in Ampel bamboo (Bambusa vulgaris). Bamboo culms were harvested from the base. Test samples were obtained from a central 2-cm long segment of each internode across the entire length of the culm. Results showed an uneven spread of vascular bundles in the internode cross-section. Transitioning from the outer to the inner layer of the internode, the number of vascular bundles per unit area decreased and their shape was variable. The size of vascular bundles in the middle layer of the internode was greater than that of those in the outer and inner layers. The shape of vascular bundles was circular in the outer layer, which gradually transformed into vertical oval toward the middle layer and horizontal oval toward the inner layer. Vascular bundles were of type III and IV in the bottom of the culm and type III in the middle to the top of the culm.

Improvement of colitis preventive effects of Gochujang by addition of Lactobacillus plantarum on C57BL/6 mice (Lactobacillus plantarum 첨가 고추장의 C57BL/6 마우스에서 대장염 예방 증진효과)

  • Park, Eui-Seong;Heo, Ju-Hee;Lim, Yaung-Iee;Ju, Jaehyun;Park, Kun-Young
    • Food Science and Preservation
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    • v.24 no.8
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    • pp.1188-1194
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    • 2017
  • Gochujang, a traditional Korean food, is fermented by mixing red pepper powder, various grain, meju and salt. Changes in the kind of ingredients and fermentation method could increase health functionalities. In this study, in vivo anti-colitis effects of gochujang prepared with mixed grains, bamboo salt baked 3 times and meju starters on DSS-induced colitis in C57BL/6 mice were studied. We prepared gochujang prepared with mixed grains (MG), bamboo salt, and Aspergillus oryzae (A) and Baccillus subtilis (B) mixed starters (MG-AB) and gochujang prepared with MG, bamboo salt and A, B and Lactobacillus plantarum (L) mixed starters (MG-ABL). MG-AB and MG-ABL significantly increased body weight and colon length compared to the control (p<0.05). MG-ABL showed significantly decreased interleukin-6 (IL-6) expression in serum compared to the control and MG-AB group (p<0.05). MG-ABL also regulated mRNA and protein levels of pro-apoptotic Bcl-2-associated X protein (Bax) and anti-apoptotic B-cell lymphoma-2 (Bcl-2) in the mice colon tissue (p<0.05). Therefore, MG-ABL exhibited the increased anticolitis effects by inhibiting damage of colon tissue, probably by regulating a pro-inflammatory cytokine of IL-6 and regulated apoptosis related genes. These results indicated that gochujang changed with good ingredients and starters had colitis preventive effects and might be due to active compounds in mixed grain and bamboo salt, and produced by L during the fermentation of gochujang.

Ultrasonic Properties of Phyllostachys bambusoides Sieb. et Zucc depending on Three directions (왕대의 3방향에 따른 초음파적 특성)

  • Kang, Seog-Goo;Lee, Hwa-Hyoung
    • Journal of the Korean Wood Science and Technology
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    • v.39 no.1
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    • pp.28-32
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    • 2011
  • This research was carried out to examine the ultrasonic properties of $Phyllostachys$ $bambusoids$ Sieb et Zucc depending on three directions for providing the fundamental properties of the Korea traditional flute, Daekeum. The ultrasonic properties of $Phyllostachys$ $bambusoids$ Sieb. et Zucc were found to be different from those of wood, because of the distinctive anatomical structure of bamboo tissue. The ultrasonic velocity of peripheral zone showed higher values of all three directions. The ratio of longitudinal velocities to perpendicular velocities showed 1.16 at peripheral zone, 1.70 at central zone, 1.38 at inner zone respectively. The ratio of radial velocities to tangential velocities were measured as 1.286 at peripheral zone, 1.325 at central zone, 0.829 at inner zone respectively.