• Journal of Microbiology and Biotechnology
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• v.31 no.12
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• pp.1643-1655
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• 2021

Recent studies have reported dysbiosis of the microbiome in breast tissue collected from patients with breast cancer and the association between the microbiota and disease progression. However, the role of the microbiota in breast tissue remains unclear, possibly due to the complexity of breast cancer and various factors, including racial and geographical differences, influencing microbiota in breast tissue. Here, to determine the potential role of microbiota in breast tumor tissue, we analyzed 141 tissue samples based on three different tissue types (tumor, adjacent normal, and lymph node tissues) from the same patients with breast cancer in Korea. The microbiota was not simply distinguishable based on tissue types. However, the microbiota could be divided into two cluster types, even within the same tissue type, and the clinicopathologic factors were differently correlated in the two cluster types. Risk of regional recurrence was also significantly different between the microbiota cluster types (p = 0.014). In predicted function analysis, the pentose and glucuronate interconversions were significantly different between the cluster types (q < 0.001), and Enterococcus was the main genus contributing to these differences (q < 0.01). Results showed that the microbiota of breast tissue could interact with the host and influence the risk of regional recurrence. Although further studies would be recommended to validate our results, this study could expand our understanding on the breast tissue microbiota, and the results might be applied to develop novel prediction methods and treatments for patients with breast cancer.

• Korean journal of applied entomology
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• v.10 no.1
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• pp.23-30
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• 1971

This work was carried out to study the chemical properties, the activity of several enzymes, chemical components and the respiratory intensity of mite galls on the leaves of Lycium chinense MILL. caused by Eriophyes kuke KISHIDA. The activities of catalase and peroxidase were higher in the gall tissue, when compared to the healthy tissue, and were increased as tile gall developed. The activity of phosphorylase of the healthy tissue seemed to be higher than that of the large gall l tissue, considering the competitive inhibition of phosphomonoeoterase and $\beta-amylase$, The activities of invertase and $\beta-amylase$ were about two times higher in the large sail tissue than those of the healthy tissue. The content of the crude protein was $20\%$ higher in the small gall tissue than that of the healthy leave tissue, and decreased as the gall matured. On the other hand, the reducing sugar level was less in the small gall tissue than that of the healthy leaves, but as the gall grew, the content of reducing sugar of the gall tissue was increased. The contents of phosphorus and tannin were increased gradually as the gall matured, and their content showed about two times higher than those of the healthy. The matured gall tissue showed higher $QO_2$ than the healthy tissue. On the other hand, the matured gall tissue presented lower $QCO_2$ than the healthy tissue, and the RQ of the healthy tissue was higher than that of the gall tissue.

• Archives of Plastic Surgery
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• v.36 no.3
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• pp.344-347
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• 2009

Purpose: Malignant soft tissue tumors, known as sarcomas, are well known to be locally aggressive, frequently metastatic, and highly recurrent. In other hands, intermediate soft tissue tumors often recur locally with adjacent tissue infiltration so the clinical management is difficult as sarcoma. In the present study, we evaluate the clinical course of the intermediate soft tissue tumors and consider the management plan for those. Methods: From March, 1998 to April 2008, total 3 patients of intermediate soft tissue tumors underwent operations. A patient with fibrohistiocytic neoplasm, a free flap reconstruction was performed, and he underwent several more operations because of recurrences. Other patients with desmoids - type fibromatosis showed local invasion and adhesion, and one of them underwent reoperation due to local recurrence. Results: 2 of 3 patients underwent recurrences of tumors and reoperations were performed. In another patient with no recurrence, follow-up period was just 5 months, so there may be recurrence of tumor in long term follow-up. Conclusion: The clinical course of intermediate soft tissue tumors shows high recurrence rate. So clinically, intermediate soft tissue tumors should be considered as sarcomas. The successful management requires wide resection, carefully planned reconstruction, and close follow up with radiologic evaluation.

• International Journal of Vascular Biomedical Engineering
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• v.3 no.1
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• pp.23-29
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• 2005

We developed a three dimensional cardiac tissue model based on human cardiac cell and mono-domain approximation for action potential propagation. The human myocyte model proposed by ten Tusscher et al. (TNNP model) (2004) for cell electrophysiology and a mono-domain method for electric wave propagation are used to simulate the cardiac tissue propagation mechanism using a finite element method. To delineate non-homogeneity across cardiac tissue layer, we used three types of cardiac cell models. Ansiotropic effect of action potential propagation is also considered in this study. In this 3D anisotropic cardiac tissue with three cell layers, we generated a reentrant wave using S1-S2 protocol. Computational results showed that the reentrant wave was affected by the anisotropic properties of the cells. To test the reentrant wave under pathological state, we simulated a hypertopic model with non-excitable fibroblasts in stochastic manner. Compared with normal tissue, the hypertropic tissue result showed another center of reentrant wave, indicating that the wave pattern can be more easily changed from regular with a concentric focus to irregular multi-focused reentrant waves in case of patients with hypertrophy.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.39 no.1
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• pp.31-34
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• 2013

In dentistry, tissue expanders have been used to obtain sufficient soft tissue for alveolar bone augmentation in the severely atrophic ridge. Herein, we review two cases of soft tissue augmentation using a self-inflating tissue expander in patients in the Department of Oral and Maxillofacial Surgery at Ewha Womans University Mokdong Hospital for bone graft and implant operations. The results of each patient were presented using pre-operative and post-operative radiographs and clinical exams. The results of our study indicate successful bone graft and implant surgery using a self-inflating tissue expander.

• Polymer(Korea)
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• v.38 no.2
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• pp.113-128
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• 2014

Tissue engineering and regenerative medicine strategies could offer new hope for patients with serious tissue injuries or end-stage organ failure. Scientists are now applying the principles of cell transplantation, material science, and engineering to create biological substitutes that can restore and maintain normal function in diseased or injured tissues/organs. Specifically, creation of engineered tissue construct requires a polymeric biomaterial scaffold that serves as a cell carrier, which would provide structural support until native tissue forms in vivo. Even though the requirements for scaffolds may be different depending on the target applications, a general function of scaffolds that need to be fulfilled is biodegradability, biological and mechanical properties, and temporal structural integrity. The scaffold's internal architecture should also enhance the permeability of nutrients and neovascularization. In addition, the stem cell field is advancing, and new discoveries in tissue engineering and regenerative medicine will lead to new therapeutic strategies. Although use of stem cells is still in the research phase, some therapies arising from tissue engineering endeavors that make use of autologous adult cells have already entered the clinic. This review discusses these tissue engineering and regenerative medicine strategies for various tissues and organs.

• Journal of Sensor Science and Technology
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• v.18 no.2
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• pp.122-127
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• 2009

We have developed and reported several micro-spikes for minimally invasive biopsy. In this paper, a micro-tissue collecting tool for tissue diagnosis extracted by micro-spike is presented. Using proposed polydimethy-siloxane (PDMS) micro-tissue collecting tool, which has a negative micro-spike structure in a porous chamber, the extracted tissue in a micro-spike is effectively detached. The gastro-intestinal tissue of a pig is extracted in an in vivo environment, and then it is detached from a micro-spike using the PDMS micro-tissue collecting tool. A fine clinical picture of the detached tissue is acquired.

• Archives of Craniofacial Surgery
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• v.21 no.3
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• pp.184-187
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• 2020

Scalp reconstruction is challenging because the scalp is inelastic, stiff, and has hair follicles. Tissue expansion offers aesthetically pleasing outcomes with minimal donor-site morbidity. However, in a scarred scalp, the extent of possible dissection for the expander insertion may be limited and surgeons must make use of the limited scalp tissue. We successfully reconstructed scarred scalps using rectangular expanders. This report presents two cases: a 4×3 cm chronic defect with widespread scarring and osteomyelitis and an 11×7.5 cm scar tissue following a skin graft. Tissue expanders were inserted in the subgaleal plane and were inflated by 195 mL and 400 mL over periods of 2 and 3 months, respectively. Subgaleal elevation of a fasciocutaneous flap was achieved with the expanded tissue. The defects were well covered, with good color, texture, and hair-bearing tissue. There were no complications involving the tissue expanders. Rectangular expanders yield more available tissue than round or crescent-shaped expanders. Moreover, since the base of the flap is well defined, the expander can be easily inserted in a limited space. Therefore, rectangular expanders are recommended for the reconstruction of scarred scalps.

• The Journal of Internal Korean Medicine
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• v.18 no.2
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• pp.94-111
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• 1997

The present experiment was designed to examine catecholamines, serotonine, amino acids, malondialdehyde and free radical scavenging activity, by administering Yukmijihwangtang extract of a variety of concentraction to senile brain. The results were summarized as followings: 1. Yukmijihwangtang significantly increased noradrenaline in the striatum, hypothalamus, midbrain and pons-medulla oblongota of the brain tissue of senile rats, and even though Yukmijihwangtang increased noradrenaline also in other brain tissue, there was no significance. 2. Yukmijihwangtang significantly increased dopamine in the striatum, hypothalamus and midbrain of the brain tissue of senile rats, and even though Yukmijihwangtang increased dopamine also in other brain tissue, there was no significance. 3. Yukmijihwangtang significantly increased serotonine in the pons- medulla oblongata and cerebellum of the brain tissue of senile rats, and even though Yukmijihwangtang increased serotonine also in the other brain tissue except hypothalamus and midbrain, there was no significance. 4. Yukmijihwangtang significantly increased amino acid in the brain tissue of senile rats. 5. Yukmijihwangtang significantly decresed malondialdehyde and free radical in the brain tissue of senile rats. According to the above results, Yukmijihwangtang is assumed to improve brain function by reacting on biochemical of the senile brain, and that Yukmijihwangtang can be used to treat regressive brain disease carrying symptoms of psychoactive disorders.

• The Journal of Korean Academy of Prosthodontics
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• v.60 no.3
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• pp.246-253
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• 2022

Flabby tissue is not rare for denture wearers. Mucostatic impression technique is necessary due to compromised retention and stability of denture resulting from distortion of mobile flabby tissue. In this report, individual tray was fabricated by model-scanning and 3D printing treatment denture. And then, mucostatic impression for flabby tissue was obtained by using individual tray modified with window technique. Definitive denture was fabricated based on information of treatment denture including incisal pontic arrangement, jaw relationship and occlusion.