• Asian-Australasian Journal of Animal Sciences
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• v.30 no.11
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• pp.1620-1632
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• 2017

Objective: This study evaluated the effects of a traditional Chinese medicine formula (TCMF) on muscle fiber characteristics in finishing pigs and the effects of the formula's extract (distilled water, ethyl acetate and petroleum ether extraction) on porcine cell proliferation and isoforms of myosin heavy chain (MyHC) gene expression in myocytes. Methods: In a completely randomized design, ninety pigs were assigned to three diets with five replications per treatment and six pigs per pen. The diets included the basal diet (control group), TCMF1 (basal diet+2.5 g/kg TCMF) and TCMF2 (basal diet+5 g/kg TCMF). The psoas major muscle was obtained from pigs at the end of the experiment. Muscle fiber characteristics in the psoas major muscle were analyzed using myosin ATPase staining. Cell proliferation was measured using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye and cytometry. Isoforms of MyHC gene expression were detected by real-time quantitative polymerase chain reaction. Results: The final body weight and carcass weight of finishing pigs were increased by TCMF1 (p<0.05), while the psoas major muscle cross-sectional area was increased by TCMF (p<0.05). The cross-sectional area and diameter of psoas major muscle fiber Ι, IIA, and IIB were increased by TCMF2 (p<0.05). The cross-sectional area and fiber diameter of psoas major muscle fiber IIA and IIB were increased by diet supplementation with TCMF1 (p<0.05). Psoas major muscle fiber IIA and IIB fiber density from the pigs fed the TCMF1 diet and the type IIB fiber density from the pigs fed the TCMF2 diet were lower compared to pigs fed the control diet (p<0.05). Pigs fed TCMF2 had a higher composition of type Ι fiber and a lower percentage of type IIB fiber in the psoas major muscle (p<0.05). The expression levels of MyHC Ι, MyHC IIa, and MyHC IIx mRNA increased and the amount of MyHC IIb mRNA decreased in the psoas major muscle from TCMF2, whereas MyHC Ι and MyHC IIx mRNA increased in the psoas major muscle from TCMF1 (p<0.05). Peroxisome proliferator-activated receptor ${\gamma}$ $coactivator-1{\alpha}$ and CaN mRNA expression in the psoas major muscle were up-regulated by TCMF (p<0.05). Porcine skeletal muscle satellite cell proliferation was promoted by $4{\mu}g/mL$ and $20{\mu}g/mL$ TCMF water extraction (p<0.05). Both $1{\mu}g/mL$ and $5{\mu}g/mL$ of TCMF water extraction increased MyHC IIa, MyHC IIb, and MyHC IIx mRNA expression in porcine myocytes (p<0.05), while MyHC Ι mRNA expression in porcine myocytes was decreased by $5{\mu}g/mL$ TCMF water extraction (p<0.05). Porcine myocyte MyHC Ι and MyHC IIx mRNA expression were increased, and MyHC IIa and MyHC IIb mRNA expression were down-regulated by $5{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). MyHC Ι and MyHC IIa mRNA expression in porcine myocytes were increased, and the MyHC IIb mRNA expression was decreased by $1{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). Four isoforms of MyHC mRNA expression in porcine myocytes were reduced by $5{\mu}g/mL$ TCMF petroleum ether extraction (p<0.05). MyHC IIa mRNA expression in porcine myocytes increased and MyHC IIb mRNA expression decreased by $1{\mu}g/mL$ in a TCMF petroleum ether extraction (p<0.05). Conclusion: These results indicated that TCMF amplified the psoas major muscle cross-sectional area through changing muscle fiber characteristics in finishing pigs. This effect was confirmed as TCMF extraction promoted porcine cell proliferation and affected isoforms of MyHC gene expression in myocytes.

• Journal of Acupuncture Research
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• v.26 no.3
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• pp.67-80
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• 2009

Objectives : The purpose of this survey is the development on the neck pain for acupuncture and moxibustion clinical guideline. Methods : The survey questionnaire was developed by the committee of experts who major in acupuncture & moxibustion or statistics for acupuncture clinical trial protocol development. The questionnaires were distributed via e-mail to 75 members of Korean Acupuncture & moxibustion society. 57 members completed answers, and the computerized data were analyzed by SPSS 17.0 statistical program. Results and Conclusions : 1. The first selected pattern identification on the neck pain This study shows that the meridian pattern identification was selected 35 times(61.4%), the pattern identification based on cause of disease was selected 8 times(14.0%), the visceral pattern identification was selected 7 times(12.3%), the other pattern identification was selected 4 times(7.0%), Qi blood yin yang pattern identification was selected 2 times(3.5%), according to symptoms was selected 1 time(2.4%). 2. Meridian pattern identification Small intestine meridian of hand Taeyang was used 39 times(18.1%), Large intestine meridian of hand Yangmyeong and Bladder meridian of foot Taeyang was used 34 times(15.7%), Gall-bladder meridian of foot Soyang was 32 times(14.8%), Tripple energizer meridian of hand Soyang was used 31 times(14.4%), Governor meridian was used 30 times(13.9%), Lung meridian of hand Taeeum was used 8 times(3.7%), Heart meridian of hand Soeum and Pericarduim meridian of hand Gworeum was used 4 times(1.9%). 3. Pattern identification based on cause of disease Wind-Cold-Dampness was used 31 times(17.5%), Accumulation of the collateral by Phelgm-Dampness was used 16 times(14.0%), affection by exopathogen Wind-Cold(stiff neck, sprain of cervical) was used 13 times (11.4%), Defecient-Cold was used 10 times(8.8%), affection by exopathogen Wind-Dampness was used 9 times(7.9%), Deep Invasion by Wind-Cold was used 8 times(7.0%), Wind-Cold was used 7 times (6.1%), Wind-Cold was used 6 times(5.3%), Accumulation in the Center by Phelgm-Dampness, Imparement of bou fluid by Pathogenic Heat, Wind-Heat with Dampness was used 5 times(4.4%), affection by exopathogen Wind-Dampness and Accumulation of the collateral by Wind-Cold was used 4 times(3.5%), Invasion of Dampness-Heat was used 2 times(1.8%). 4. Visceral pattern identification Rising of the Liver yang was used 16 times(41%), Yin deficiency of Liver and Kidney+pathogens was used 15 times(38.5%), Yin deficiency of Liver and Kidney was used 8 times(20.5%) on this survey.

• Clinical and Experimental Pediatrics
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• v.51 no.12
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• pp.1315-1319
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• 2008

Purpose : We aimed to examine the effect of rooming-in care on newborn infants emotional stability by comparing them with those cared for in a nursery. Methods : Forty-eight full-term newborn infants born at Cheil General Hospital between July 1 and October 31, 2007, were enrolled. Twenty-four newborn infants were roomed-in in their mothers rooms (rooming-in group), and 24 newborns were cared for in the hospital nursery (the nursery group) for the first 3 days of their lives. Those with perinatal problems that required medical treatment were excluded. By using Brazeltons neonatal behavior assessment scale, we measured irritability and self-quieting as well as the duration of crying after heel-stick puncture for the newborn metabolic screening test. Results : The rooming-in group had a higher irritability score than the nursery group ($6.8{\pm}1.7$ vs. $4.2{\pm}2.1$, P<0.001), thereby suggesting stable behavior against external irritation; the former also had a higher self-quieting activity score ($5.9{\pm}0.3$ vs. $4.5{\pm}1.8$, P=0.001), thereby suggesting that stability was reached quickly from the irritated state. Time taken to stop crying after the heel-stick puncture was significantly shorter in the rooming-in group than in the nursery group ($17{\pm}15.1$ seconds vs. $115.3{\pm}98.5$ seconds, P<0.001). Conclusion : These results show that newborn infants in the rooming-in group exhibit more stable behavior against external irritation and can be stabilized from an irritated state more quickly than infants cared for in the nursery, even after a few days of rooming-in care.

• Korean Journal of Weed Science
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• v.10 no.2
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• pp.114-121
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• 1990

Inactivation in soil absorption, translocation of 2, 4-D by plants vary depending upon soil environments and herbicide formulations. Experiment was conducted in a glasshouse using rectangular pots($1350cm^2$) to evaluate the growth responses of barnyardgrass (Echinochloa crus-galli) and Indian jointvetch (Aesehyrcomene indica) to two formulations of 2, 4-D. The formulations used were 40% 2, 4-D amin salt (2, 4-D/AS) and 19.7% complex of rice husk lignin and 2, 4-D (2, 4-D/LG) which were applied at 200g ai/ha. Soil environments included fertilizer levels, soil pH, organic matter contents, and soil textures, Each treatment was replicated three times. The herbicidal activity of 2.4-D increased and lasted with increased levels of fertilizer. The activity also increased and lasted with low soil pH and decreased content of organic matter. Generally 2, 4-D/LG showed higher and longer herbicidal activity than 2. 4-D/AS for both test plants under all conditions applied. However, the herbicidal activity was influenced by the formulations more than by soil textures. It was thought that 2, 4-D/AS was released in a short time and inactivated readily while 2, 4-D/LG was slowly released and gave an opportunity of absorption by plants for a long period.

• Journal of Life Science
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• v.24 no.4
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• pp.370-376
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• 2014

Although the grasshopper Oxya chinensis sinuosa has long been used as food in Korea, there is little data on its functional effects. In this study, we investigated the anti-inflammatory effect of O. c. sinuosa ethanol extract (OCE) in RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for induction of inflammation. First, we determined that there is no cytotoxicity at $2,000{\mu}g/ml$ or less of OCE in RAW 264.7 cells. To evaluate the anti-inflammatory effects of OCE, we investigated expression levels of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-${\alpha}$ and interleukin (IL)-6, and pro-inflammatory enzymes such as inducible nitric oxide synthase (iNOS) and cyclo-oxygenase-2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether OCE could inhibit translocation of NF-${\kappa}B$ p65 into the nucleus in LPS induced RAW 264.7 cells. As a result, we found that the mRNA and protein levels of TNF-${\alpha}$ and IL-6 decreased in LPS-induced RAW 264.7 cells after treatment with OCE in a dose-dependent manner. In addition, we confirmed a $2,000{\mu}g/ml$ concentration of OCE inhibited translocation of NF-${\kappa}B$ p65 by immunnostaining and Western blot analysis, and a decrease in the protein expression levels of iNOS and COX-2. Accordingly, we suppose that OCE has an anti-inflammatory effect through down-regulation of TNF-${\alpha}$, IL-6, iNOS, and COX-2 related to ${\kappa}B$ p65 inflammatory signaling pathways.

• Korean Journal of Food Science and Technology
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• v.25 no.5
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• pp.468-474
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• 1993

A dehydrating sheet comprises polymeric water absorber. which are packed in a semipermeable cellophane film bag allowing selective permeation of water. This sheet dehydration is quite different from conventional drying method such as sun drying, hot-air blast drying and cold air blast drying in a sense that samples are dried without heat treatment. As a part of the studies to develope a new processing method for effective utilization of dark muscle fishes, the preparation of a good quality salted and semi-dried mackerel by the dehydrating sheet was attempted. The dehydration time for preparation of a salted and semi-dried mackerels containing approximately equal moisture content were revealed $180{\sim}510min$ in conventional drying method and $90{\sim}160min$ in this sheet dehydration, respectively. The moisture and histamine contents of those salted and semi-dried mackerels were $59.4{\sim}62.4%$ and $2.5{\sim}3.6 mg/100g$, respectively. The changes in peroxide value, fatty acid composition, brown pigment formation, myofibrillar protein solubility and Ca-ATPase activity during processing of the salted and semi-dried mackerel prepared by the sheet dehydration were more lower than those of products prepared by conventional drying methods. Therefore, these result showed that the quality of a salted and semi-dried mackerel prepared by the sheet dehydration was imperial to that of those products by conventional drying method.

• Korean Journal of Organic Agriculture
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• v.19 no.1
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• pp.65-82
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• 2011

In order to evaluate the weed suppressing effect of Solanum viarum Dunal. In this study species diversity in patch of S. viarum and allelopathic effects of the aqueous extracts on S. viarum were investigated. Number of species and species diversity in site close to patch of S. viarum were decreased gradually 1site ($7.7{\pm}2.0,\;1.5{\pm}0.2$), 2site ($5.3{\pm}1.2,\;1.2{\pm}0.2$) and 3site ($4.0{\pm}1.7,\;0.9{\pm}0.1$). And total phenolic compounds of soil in survey area were increased gradually site1 ($0.16{\pm}0.01mg\;g^{-1}$), site2 ($0.17{\pm}0.01mg\;g^{-1}$) and site3 ($0.22{\pm}0.02mg\;g^{-1}$). So the number of species and species diversity (r=-0.692, P<0.05) were negatively correlated with increased total phenolic compounds of soil in the survey area. The relative germination ratio, the mean germination time, the relative elongation ratio, the fresh weight and the dry weight of receptor plants are generally getting decreased while the concentration of the aqueous extracts from S. viarum escalate. But every ratio was various depending on the growing regions, the kind of receptor plants and the treatment of the aqueous extracts. Especially, the radicle by injection of the aqueous extracts concentration of S. viarum was influenced more than the shoot on the same condition. The total phenolic compounds on region of S. viarum was gradually increased in stems (fresh $0.56{\pm}0.02mg\;g^{-1}$, dry $1.58{\pm}0.08mg\;g^{-1}$), roots (fresh $1.77{\pm}0.07mg\;g^{-1}$, dry $2.64{\pm}0.06mg\;g^{-1}$), leaves (fresh $6.01{\pm}0.14mg\;g^{-1}$, dry $7.04{\pm}0.29mg\;g^{-1}$), seeds (fresh $6.21{\pm}0.17mg\;g^{-1}$, dry $9.08{\pm}0.73mg\;g^{-1}$) in order. On the contrary, the negative correlation on germination and growth of receptor plants was shown by total phenolic compounds on the each parts of S. viarum. We think that the aqueous extracts of S. viarum showed allelopathic effects on other plants. Therefore, S. viarum holds the higher competitiveness in plant community in Jeju Island and makes possibility of application as natural herbicide.

• Journal of Mushroom
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• v.10 no.4
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• pp.184-190
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• 2012

Monitorings and management experiment of adult Lycoriella ingenua (Diptera: Sciaridae) in artificial sawdust grown shiitake mushroom cultivation were carried out by using yellow sticky traps and Hypoaspis aculeifer (Acari: Laelapidae) at Hwaseong, Buyeo and Cheongwon in 2012. The larvae of L. ingenua reduced commercial value of the mushroom by direct eating or retarding the growth of mushroom by spongifying the sawdust medium. The number of L. ingenua caught by traps showed the highest peak in late september resulting in 127.5~1,025.7, 87.4~743.6 and 133.7~650.4 individuals per trap in Hwaseong, Buyeo and Cheongwon, respectively. Damage rate of fruiting bodies in shiitake mushroom by L. ingenua were 7.7~30.3% in Hwaseong, 6.7~25.3% in Buyeo and 5.3~26.0% in Cheongwon and showed the highest peak in late september. L. ingenua were effectively controlled when 30.3 individuals of H. aculeifer per $m^2$ inoculated for three time with 7 to 14 days of interval. In Hwaseong, Buyeo and Cheongwon, density of L. ingenua which were caught on the yellow sticky traps were 168.2, 126.1, 132.5 individuals, respectively. And, damage rate of fruiting bodies by L. ingenua were 5.9%, 5.5% and 5.4% respectively. Both of the L. ingenua density and damage rate of mushroom reduced more than 60% in all experimental site in contrast to the control treatment.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.7
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• pp.790-800
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• 2017

As men get older, total testosterone levels decline gradually, and concentrations of free and bioavailable testosterone decline sharply with each decade beyond their 30s. Andropause or testosterone deficiency syndrome (TDS) is defined as a decrease in sexual satisfaction or decline in general well-being accompanied by low levels of testosterone in older men. This male climacteric is characterized by nervousness, reduced potency, decreased libido, irritability, fatigue, depression, memory problems, sleep disturbances, and hot flushes. Cirsium japonicum (CJ) is used as a traditional medicine for hemorrhage, blood congestion, and inflammation in Korea. However, there is no report on the efficacy of CJ treatment for TDS. In this study, we observed the mitigating effect of CJ extract (CE) and fermented CJ extract (FCE) on symptoms of TDS. In elderly male rats, total and testosterone levels, hind limbs muscles, forced swimming time, and total and motile sperm counts significantly increased after daily intake of CE and FCE for 6 weeks. In contrast, sex hormone binding globulin, retroperitoneal fat, total serum cholesterol, and triglyceride levels were significantly reduced in CE and FCE groups. However, there was no difference in prostate specific antigen, aspartate aminotransferase, and alanine aminotransferase levels among all groups, which means CE and FCE did not have putative adverse effects. In a cell experiment, we also observed that CE and FCE enhanced expression of genes related to testosterone biosynthesis but reduced genes involved in testosterone conversion. On the whole, these positive effects on TDS were greater in FCE compared to CE. Thus, these results suggest the potential of FCE as a promising natural product for recovering testosterone levels and alleviating TDS.

• Journal of the korean academy of Pediatric Dentistry
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• v.26 no.4
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• pp.677-687
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• 1999

This study was performed to evaluate the effect of aging and thermal cycling on the tensile strength of six commercially available glass-ionomer materials: two chemically set glass-ionomer materials(Fuji II, Fuji IX), two resin-modified glass-ionomer materials(Fuji II LC, Vitremer), and two polyacid-modified composite resins(Compoglass, Dyract). Rectangular tension test specimens were fabricated in a teflon mold giving 5mm in gauge length and 2mm in thickness. All samples were divided into 3 groups. Group 1 was immersed in a $37^{\circ}C$ distilled water for 1 hour. Group 2 was immersed in a $37^{\circ}C$ distilled water for 30 days. Group 3 was subjected to 10,000 thermal cycles between $5^{\circ}C$ and $55^{\circ}C$, and the immersion time in each bath was 15 seconds per cycle. Tensile testing was carried out at a cross-head speed of 0.5mm/min and fracture surfaces were examined with scanning electron microscope. The results obtained were summarized as follows; 1. The polyacid-modified composite resins were stronger than the resin-modified glass-ionomer materials, which were much stronger than the conventional glass-ionomer materials. 2. Tensile strengths were slightly increased after aging treatments for 30days. 3. Tensile strengths of conventional glass ionomers were significantly increased after thermal cycling treatment(p<0.01). 4. The highest tensile strength value of 45.4MPa was observed in the Dyract group and the lowest value of 13.3MPa was observed in the Fuji II LC group after the thermal cycling test, and the strengths of polyacid-modified composite groups were significantly higher than those of other groups. 5. The highest characteristic strength value of 48.6MPa was obtained in the Dyract group, however the highest Weibull modulus value of 8.9MPa was obtained in the Compoglass group after thermal cycling test.