• Animal cells and systems
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• v.7 no.1
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• pp.89-92
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• 2003

Immunoassays have become indispensable tools and achieved great importance in scientific and medical research. However, typical immunoassays are time-consuming and use complex, multi-step procedures. In this study, we introduce a new immunoassay system for the quantification of several analytes at a time without any washing steps. It is comprised of a detector solution with fluorescence-labeled antibodies and a test strip with immobilized capture antibodies. Using a micro-array scanner, the antigen-antibody complex was quantitatively determined by measuring the intensities of fluorescence on the capture lines or dots of nitrocellulose membrane. This method demonstrated its rapid quantitative determination of analytes without many processing steps as well as specific identification of multiple analytes in biological specimens.

• Bulletin of the Korean Chemical Society
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• v.17 no.4
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• pp.338-342
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• 1996

A sensitive method for the determination of trace cadmium after the preconcentration by extracting its 8-hydroxyquinoline complex into a molten benzophenone was developed. Several experimental conditions such as the pH of solution, the amounts of 8-hydroxyquinoline and benzophenone, stirring time, and standing time were optimized. Trace cadmium in 100 mL water sample was chelated with 2.5 mL of 0.001 M 8-hydroxyquinoline at pH 8.0. After 0.07 g benzophenone was added, the solution was heated to about 70 ℃ and stirred vigorously for 1 minute to dissolve the complex quantitatively in a molten benzophenone, and stood for 30 minutes to reproduce the microcrystalline benzophenone. The benzophenone containing Cd-8-hydroxyquinoline complex was filtered and dissolved in acetone. Cadmium was determined by a flame atomic absorption spectrophotometry. The interfering effects of diverse concomitant ions were investigated and eliminated. This method could be applied to natural water samples and the recovery of more than 90% was obtained in the real samples.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.404.1-404.1
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• 2002

A LC/MS/MS method for the simultaneous determination of the activities of seven major human drug-metabolizing cytochrome P450s (CYP3A4. CYP2D6. CYP2C9. CYP1A2, CYP2C19, CYP2A6. and CYP2C8) was developed. This method used an in vitro cocktail of specific substrates (midazolam. bufuralol. diclofenac, ethoxyresorufin. S-mephenYlOin. coumarin. and paclitaxel) and LC/MS/MS. The assay incubation time is 20 min and the analysis time is 8 min/sample. (omitted)

• Journal of Positioning, Navigation, and Timing
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• v.13 no.2
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• pp.149-158
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• 2024

Global Navigation Satellite System (GNSS) receivers are becoming increasingly sophisticated, equipped with advanced features and precise specifications, thus demanding efficient and high-performance hardware platforms. This paper presents the design and implementation of a Field-Programmable Gate Array (FPGA)-based GNSS receiver development platform for multi-band signal processing. This platform utilizes a FPGA to provide a flexible and re-configurable hardware environment, enabling real-time signal processing, position determination, and handling of large-scale data. Integrated signal processing of L/S bands enhances the performance and functionality of GNSS receivers. Key components such as the RF frontend, signal processing modules, and power management are designed to ensure optimal signal reception and processing, supporting multiple GNSS. The developed hardware platform enables real-time signal processing and position determination, supporting multiple GNSS systems, thereby contributing to the advancement of GNSS development and research.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.10
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• pp.1438-1442
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• 2003

A method for the determination of nitrogen in ruminant feedstuffs, products and excreta (e.g. milk and urine) using a spectrophotometer is developed, where samples processed for P determination are also used to determine N. Samples are digested with sulphuric acid and subsequently with hydrogen peroxide in Kjeldahl tubes. Digested solutions along with phenol and buffered alkaline hypochlorite reagents are incubated in a water bath at $37^{\circ}C$ for 30 min and presented in the spectrophotometer. The spectrophotometer set at 625 nm measures the concentration of N of each sample. Nitrogen in 261 of the samples was also determined by the classical Kjeldahl method in order to develop a relationship between N determined by the Kjeldahl method (Y) and the colorimetric method (X). The mean value of Y was as high as that of X (0.92 vs. 0.96; p>0.05). The colorimetric method predicted Kjeldahl N highly significantly (Y=0.985X-0.024, $R^2=0.993$, p<0.001; or more simply Y=0.974X, $R^2=0.993$, p<0.001). An analysis of regression found no difference (p>0.05; both t-test and F-test) between colorimetric (0.96% N) and adjusted (0.96% N) N. In comparison with the Kjeldahl method, the analytical capacity of N by colorimetric method increases greatly, where 200-300 determinations of N are possible in a working day. In addition, the system provides an opportunity to use not only the same digested solution for both N and P determination of a particular sample, but also uses the same spectrophotometer to assay both N and P. Therefore, the system may be attractive in situations where both elements of a sample are to be determined. In conclusion, the speed of N determination, low cost, efficient use of labour, time and reagents, fewer items of equipment, and the reduction of environmental pollution by reducing effluent and toxic elements are the advantages of this method of N determination.

• The Journal of the Korea Contents Association
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• v.17 no.12
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• pp.516-524
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• 2017

This study was to examine the effectiveness of Self-Determination Group Program for children with disabilities in Korea by using method of meta-analysis. For the purpose of the study, master's theses, doctoral dissertations, and journal articles published in Korea up to June, 2017 were systematically reviewed. As a result, a total of 17 studies were eligible for the inclusion criteria. The mean effect sizes and test for homogeneity of effect size(Q-statistic) were analyzed by using Comprehensive Meta-Analysis software 2.0. The main findings of the study were as follows. First, the average effect sizes for Self-Determination Group Program were ES=1.695 of Self-Determination, ES= 1.316 of Social Skills. Second, the moderate variables of the effect size for Self-Determination Group Program was 'age' of 'a type of disability', 'sessions', 'the number of sessions within a week', 'time of one session' and 'a major of the director'. Based on the study results, the research and practice implications were discussed.

• Journal of Korean Society for Geospatial Information Science
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• v.23 no.4
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• pp.67-74
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• 2015

GNSS/Leveling technology makes it possible to get geoidal height geometrically using GNSS and Leveling technology. GNSS/Geoid technology refers to a technology for obtaining orthometric height by subtracting geoidal height achieved by Geoid technology from ellipsoidal height achieved by GNSS technology. The purpose of this study is to verify the accuracy of the ellipsoidal height determination in order to verify the accuracy of the orthometric height determination by the GNSS/Geoid technology. For the study, a test bed was selected in Kyungnam province and GNSS Static surveying was accomplished in the test bed and then the GNSS data was processed in accordance with various analysis conditions. So, it was verified the accuracy of the ellipsoidal heights determination in accordance with the surveying conditions under the GNSS Static surveying. According to the research results, to ensure the 3cm goal accuracy of the ellipsoidal height determination, it should be surveyed by four fixed points on the survey area periphery and more than two hours of the GNSS occupation time, And also, it was found that should be limited to a baseline distance of 20km under the GNSS Static surveying.

• Journal of Animal Reproduction and Biotechnology
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• v.34 no.2
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• pp.111-116
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• 2019

This study was conducted to analyze the specific genes associated with sex-determination in Korean native cow. The highly organized spermatogenesis requires accurate spatial and temporal regulation of gene expression, which is governed by transcriptional, post-transcriptional, and epigenetic processes. Recently, farmers have been interested in determining the sexual identity of the calves in their farm. We analyzed the sperm of Korean native and Holstein cows, which were supplied from Hanwoo Improvement Center. We evaluated sperm motility and expression of sperm-specific genes after treating semen with both male- and female reagents. Sperm motility in Korean native cows decreased by approximately 10% in the first 30 minutes after treatment with sex-determination reagent. However, sperm motility of Holstein cows decreased to 60-70% after 15 minutes and to 20-30% after 30 minutes. We selected six specific genes expressing in the spermatozoa to analysis the gene expression level. The Real-time PCR results suggest that the selected genes (Gimap4, Tmeff1, Rac2, Abi2, Rac1, and Clu) were highly expressed in the group treated with the male reagent compared to the group treated the female reagent and to the untreated-group (control). In the present study, we suggest that the selected genes play a pivotal role in sex-determination.

• Nuclear Engineering and Technology
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• v.48 no.3
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• pp.727-732
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• 2016

A simple and rapid spectrophotometric method has been developed to enable the determination of plutonium concentration in an irradiated fuel solution in the presence of all fission products. An excess of ceric ammonium nitrate solution was employed to oxidize all the valence states of plutonium to +6 oxidation state. Interference due to the presence of fission products such as ruthenium and zirconium, and corrosion products such as iron in the envisaged concentration range, as in the irradiated fuel solution, was studied in the determination of plutonium concentration by the direct spectrophotometric method. The stability of plutonium in +6 oxidation state was monitored under experimental conditions as a function of time. Results obtained are reproducible, and this method is applicable to radioactive samples resulting before the solvent extraction process during the reprocessing of fast reactor spent fuel. An analysis of the concentration of plutonium shows a relative standard deviation of <1.2% in standard as well as in simulated conditions. This reflects the fast reactor fuel composition with respect to uranium, plutonium, fission products such as ruthenium and zirconium, and corrosion products such as iron.

• Analytical Science and Technology
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• v.24 no.3
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• pp.231-235
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• 2011

A spectrofluorimetric method for the determination of EDTA in real samples such as mayonnaise, powder detergent and cleansing cream with tiron (4,5-dihydroxy-1,3-benzenedisulfonic acid) as a fluorimetric reporter was developed. When tiron is chelated with Cu(II), the fluorescent intensity is decreased by a quenching effect. However, when Cu(II)-tiron chelate reacts with EDTA, fluorescent intensity is increased as tiron is released. Several experimental conditions such as pH of the sample solution, the amount of Cu(II), the amount of tiron, heating temperature and heating time were optimized. Fe(III) interfered more seriously than any other ions, interference of Fe(III) could be disregarded, because Fe(III) was scarcely contained in selected real samples. The linear range of EDTA was from $8.0{\times}106{-8}\;M$ to $2.0{\times}10^{-6}\;M$. With this proposed method, the detection limit of Fe(III) was $5.2{\times}10^{-8}\;M$. Recovery yields of 92.7~99.3% were obtained. Based on experimental results, it is proposed that this technique can be applied to the practical determination of EDTA.