Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2002.10a
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- Pages.404.1-404.1
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- 2002
Simultaneous determination of seven major human cytochrome P450 activities using LC/MS/MS
- Lee, Seung-Seok (College of Pharmacy. Wonkwang University) ;
- Kim, Hae-Kyoung (LGC) ;
- Jin, Joon-Ki (LGC) ;
- Lee, Hye-Won (LGC) ;
- Kim, John (LGC) ;
- Lee, Hye-Suk (LGCI)
- Published : 2002.10.01
Abstract
A LC/MS/MS method for the simultaneous determination of the activities of seven major human drug-metabolizing cytochrome P450s (CYP3A4. CYP2D6. CYP2C9. CYP1A2, CYP2C19, CYP2A6. and CYP2C8) was developed. This method used an in vitro cocktail of specific substrates (midazolam. bufuralol. diclofenac, ethoxyresorufin. S-mephenYlOin. coumarin. and paclitaxel) and LC/MS/MS. The assay incubation time is 20 min and the analysis time is 8 min/sample. (omitted)
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