• Toxicological Research
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• v.19 no.1
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• pp.13-19
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• 2003

GX-12 is a naked DNA vaccine developed by the DongA Pharmaceutical Co. Ltd. and Genexine for the treatment of HIV infection. This study was peformed to evaluate the biodistribution and expression of GX-12 mRNA in gonadal tissues, and to investigate the histopathological changes after the repeated intramuscular injection. GX-12 (400 $\mu\textrm{g}$/head) was injected into the left anterior tibialis once a week for four weeks. On day 1, 5, 15, 30 and 45 after the final administration, gonadal tissues (testes, epididymis, seminal vesicles, penis, prostate glands, ovaries, vagina, uterus) and the injection site (muscle) were harvested and examined for the expression of mRNA by RT-PCR. In addition, histopathological examination was peformed at each time point. At the injection site, mRNA expression of GX-12 was detected only at early time points (1 ~ 15 days after injection) but not thereafter. However, in gonadal tissues, mRNA expression was not identified at all time points both in male and female rats. There were no histopathological changes in all reproductive organs and muscle. Based on these results, it is unlikely that the plasmid DNAs of GX-12 was distributed to- and expressed in gonadal tissues, suggesting that the chance of germline integration and transmission is negligible.

• Journal of the Korean Society for Precision Engineering
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• v.30 no.3
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• pp.331-339
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• 2013

The Interest in disease prevention and rehabilitation is increasing depending on increase of patients with spinal. This is being developed using the spine stabilization device is being studied. So far studies have only evaluated the effect on trunk stabilization exercises but analysis of human movement patterns for active movement and passive movement did not. We assessed the muscle activity of trunk and leg muscle during passive and active tilt mode on eight tilt directions at tilt angle of $30^{\circ}$ using 3-D dynamic postural balance training system. We performed experimental study on the muscular activities of trunk muscle about rectus abdominis, external obliques, latissimus dorsi, erector spinae, and leg muscle about rectus femoris, Biceps femoris, Tibialis Anterior, gastrocnemius. As a result, muscle activation was different depending on the direction of movement and pattern. The results indicate that various patterns of spinal stabilization exercise system could be applied to an effective training of chronic low back pain patients.

• The Journal of Korean Physical Therapy
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• v.21 no.1
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• pp.19-25
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• 2009

Purpose: We investigated the changes that effects of load stimulation (LS) on static balance and muscle activities in normal healthy elders and chronic stroke patients. Methods: Subjects were instructed twenty normal healthy elders (8 men and 12 women) and ten chronic stroke patients (4 men and 6 women). They were tested while standing on a force platform under two conditions; LS, no LS were applied in random order. Prior to testing, adhesive surface EMG electrodes were fastened to the skin overlying the right (non-paralytic side in stroke patient) bellies of tibialis anterior (TA) and lateral gastrocnemius (LG) muscles. They were assessed on postural sway and %MVC (Maximum voluntary contraction) of TA and LG. Results: Participants showed that the application of LS brought about a decrease in postural sway as expressed by average sway path and velocity. %MVC of TA and LG were increased. The application of LS to normal and stroke patients decreases postural sway during quite stance. Conclusion: These findings the application of LS to normal and stroke patients improves static balance.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.9 no.4
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• pp.331-337
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• 2015

This research was to develop the cycling system of lower limbs for rehabilitation during cycle exercise in supine position. Also we analyzed the muscular activity of lower-limbs at various exercise conditions according to exercise mode, load, velocity. 42 healthy subjects(ages 20-60 years) were participated. We measured the muscular activities of right lower limb muscle in rectus femoris, biceps femoris, tibialis anterior, medial gastrocnemius, soleus. Results, medial gastrocnemius shows high value on load 10 stage than load 1 and 5 stage. And all muscular activity except medial gastrocnemius was decreased as increase of velocity. We have found that there is a difference of lower limbs activity depending on exercise mode and method. This study could be applied to reference data to develop cycle system of lower limbs for rehabilitation.

• PNF and Movement
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• v.15 no.3
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• pp.303-310
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• 2017

Purpose: This study observed the activity of trunk and lower limb muscles during a modified bridging exercise with various weight loads. Methods: The participants in this study consisted of 15 male adults. The muscle activity of the elector spinae, rectus abdominis, gluteus maximus, gluteus medius, vastus medialis, vastus lateralis, tibialis anterior, and soleus muscles was measured with an EMG-8 system during a modified bridging exercise performed with various weight loads (indicated as percentage of body weight). Differences in muscle activity during the bridging exercise according to the weight load applied were analyzed using a one-way ANOVA, and post hoc analysis was performed using LSD. Statistical significance was accepted at a p-level of 0.05. Results: When the subjects performed the modified bridging exercise with various weight loads, the muscle activity of the gluteus maximus and vastus medialis peaked at a load of 0.5%. The activity of the gluteus medius showed a remarkable difference when the modified bridging exercise was performed at loads of 0% and 0.5%, 0% and 1%. In addition, the activity of the vastus medialis showed a remarkable difference between modified bridging exercises performed at a load of 0% and that performed at a load of 0.5%. Conclusion: The results suggest that performing modified bridging exercises with a load of 0.5% of body weight results in significant differences in the activity of the gluteus medius and vastus medialis muscles. Thus, it is suggested that performing the modified bridging exercise at 0.5% of body weight may selectively strengthen the gluteus medius and vastus medialis muscles.

• Archives of Plastic Surgery
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• v.33 no.2
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• pp.181-186
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• 2006

Myocutaneous flaps have improved the management of soft tissue defects on buttocks and lower extremity. However, there are several inherent disadvantages of muscle flaps such as functional deficits of the donor sites and the bulkiness at the recipient site. To overcome these disadvantages, we have used perforator-based fasciocutaneous rotation flaps for reconstruction of the buttock and lower extremity defects. From March 2003 to February 2005, we have treated 14 patients using perforator-based fasciocutaneous rotation flaps. 10 flaps were based on perforators of the gluteus maximus muscle, and 4 flaps were nourished by perforators from the tibialis anterior and posterior system. The mean postoperative follow-up period was about 1 year. The technique involves localization of the flap perforators preoperatively with a Doppler. The flaps were elevated superficial to the fascia with preservation of one to three perforators. The donor site is then closed primarily. All flaps completely survived and there was no perioperative complications. There was no functional disability of the donor area with esthetically pleasing results. Perforator-based fasciocutaneous rotation flaps for the reconstruction of buttock and lower extremity defects are excellent alternatives to musculocutaeous flaps. The vascularity of the flaps is robust and dissection is technically easy. Perforator flaps do not require sacrificing muscles, but provide sufficient volume and are durable Furthermore, these flaps result in less scar formation and allow more liberal dissection with safety. We conclude that perforator-based fasciocutaneous rotation flaps are very useful for reconstruction of the buttock and lower extremity.

• The Journal of Internal Korean Medicine
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• v.40 no.6
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• pp.1136-1144
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• 2019

Objective: Physical inactivity contributes to mortality rates and is now the fourth most frequent cause of death worldwide. Red ginseng is a medicinal herb that is often used as an ergogenic aid. In this study, red ginseng was administered to rats to test whether it affected their ability to exercise. Methods: Forty-five rats were randomly distributed and divided into five groups: normal (N, n=5), control (C, n=10), the group to which only red ginseng was administered (H, n=10), the group to which only amino acid complex was administered (A, n=10), and the group to which both red ginseng and amino acid complex were administered (HA, n=10). Once a day for three weeks, 333.3 mg/kg body weight per day (b.w./day) of red ginseng and 750 mg/kg b.w./day of amino acid preparation were administered to rats. After three weeks, body weight, swimming time, and the weight of the anterior tibialis muscle of rat were measured. Blood was taken for analysis using the cardiac puncture method. Results: The swimming time of group H (921.3±199.26 sec) showed significant improvement compared to that of group C (798.48±156.37 sec) (p<0.05). Conclusion: Red ginseng has improved swimming time in rat and can be used as an effective ergogenic aid.

• Journal of the Korean Society of Physical Medicine
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• v.7 no.4
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• pp.471-480
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• 2012

PURPOSE: The object of the present study is to investigate the effects of the insole supporting medial longitudinal arch while walking in spastic cerebral palsy with pes planus. METHODS: Ten spastic bilateral cerebral palsy children with pes planus participated in this study. The insole were custom-made for the individual child. Muscle activity was measured by surface EMGs attached on tibialis anterior (TA), gastrocnemius (GA), vastus medialis oblique (VMO), biceps femoris long head (BF). temporal-spatial parameters such as velocity, step length, stride length, stance time, toe angle were collected while the subjects walked on the GAITRite system. RESULTS: The results of the present study were summarized as follows: 1. Muscle activities in mean EMGs while walking: Left VMO, Right TA, Left BF and GA revealed significant reductions after applying insole. 2. Muscle activities in peak EMGs while walking: Left TA and BF demonstrated reductions significantly after applying insole. 3. There were improvements in temporal-spatial gait parameters with insole: velocity, both step length, Right stride length and Right toe angle were increased(p<.05). CONCLUSION: Therefore the current study demonstrated that insole supporting the medial longitudinal arch would be effective on gait of the spastic cerebral palsy with pes planus.

• Journal of the Korean Society of Physical Medicine
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• v.9 no.3
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• pp.243-248
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• 2014

PURPOSE: This study was aim to the change of muscle activities of lower extremity and waist during lifting a small object on the floor according to different foot position of women in their twenties wearing a skirt. METHODS: 9 women in their twenties wearing a skirt were selected and were measured the muscle activities of medial gastrocnemius (MG), tibialis anterior (TA), vastus lateralis (VL) and iliocostalis (IC) when they lifted a small object on the floor. The two different foot position employed were "both feet posed straight side by side" (condition 1) and "both feet posed diagonally to 45 degree" (condition 2) used. The order of feet position was selected randomly and the subject took a rest for 30 min between tests to prevent muscle fatigue. We calculated the mean and standard deviation of muscle activities and used Mann-Whitney U test to compare the difference between the two foot positions with SPSS(IBM Korea) RESULTS: The muscle activity of condition 2 was greater than that of condition 1 in right side of TA, VL, and IC and left side of TA, VL, MG and IC. The right side of TA, VL and left side VL were significant difference between condition 1 and condition 2(p<.05). CONCLUSION: We suggest "both feet posed straight side by side" position is better if a woman wearing a skirt lift the small object and it will help prevent the low back and lower limb problems in the future.

• Physical Therapy Korea
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• v.11 no.2
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• pp.17-25
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• 2004

The purpose of this study was to assess the effect of applied insole types to lower extremities muscle fatigue during treadmill exercise. The control group and each different insole type group consisted of ten healthy male subjects. In the control group and each different insole type (soft type; 10 shore, semi-rigid type; 33 shore, rigid type; 50 shore) treadmill exercise was performed in twenty-five minutes. The electromyography (EMG) signals of four muscle (tibialis anterior, gastrocnemius medialis, rectus femoris, biceps femoris) were recording at sampling rate of 1024 Hz during treadmill exercise. The localized muscle fatigue (LMF) can be investigated using power spectral analysis. When did data analysis that excepted initial five minutes. The raw EMG signals was processed using the fast Fourier Transformation (FFT) and the median power frequency value was determined in initial ten second period and in last ten second period. Fatigue index was calculated and collected data were statistically analyzed by SPSS version 10.0 two-way using analysis of variance (ANOVA) with repeated measures ($4{\times}4$) was used to determine the main effect and interaction. Post hoc was performed with least significant difference. A level of significance was .05. Muscles fatigue index were significantly decreased in insole types (p<.05) and not significantly different in muscle (p>.05). Post hoc analysis shows that fatigue index in soft insole type, semi-rigid insole type and rigid insole type were lower than that control group (p=.028, p=.146, p=.095). There were no interaction between insole type and muscles (p>.05). The finding of this study can be used as a fundamental data when insole is applied and insole can be used to decreased of a fatigue during the dynamic exercise.