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Physicochemical Properties of Dioscorea opposita Thunb. Solution Fermented by Lactobacillus bulgaricus

  • Park, Ju-Hyun;Ahn, Joung-Jwa;Kwak, Hae-Soo
    • Food Science of Animal Resources
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    • v.30 no.5
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    • pp.773-779
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    • 2010
  • The present study was designed to determine the physicochemical properties of yam (Dioscorea) solution fermented by L. bulgaricus, the protective effect of fermented Dioscorea opposita Thunb. on stomach lesions, and the possibility of its application to fermented dairy products. When the concentration of yam increased, the pH increased in raw powder samples but not in extracted powder samples. In 6% raw powder containing the sample, the viable cell counts showed a dramatic increase, whereas those in extracted yam powder significantly decreased in all concentrations during 32 h of fermentation. The viscosity of extracted yam powder showed a lower value than that of raw powder, which was maintained throughout the fermentation. The solution of Dioscorea opposita Thunb. raw powder showed a significantly higher allantoin content compared with the extracted powder (p<0.05). At 2% of added Dioscorea opposita Thunb., 0.65 mg/mL was contained in both samples; however, when 6% of Dioscorea opposita Thunb. was added, the amount of diosgenin increased up to 1.9 mg/mL. When 200 mg of powder/kg body wt. was injected, gastric lesions were apparently reduced compared with those of control rats. With 100 mg/kg body wt. injected into rats, the protective effect was a little lower than that in 200 mg/kg body wt. Based on these data, the present study indicated that yam powder fermented by L. bulgaricus showed a protective effect on gastric lesions in rats; therefore, it may be included as an added ingredient in yogurt manufacture.

Anti-oxidative Activity of the Extracts from Houttuynia cordata Thunb. Fermented by Lactic Acid Bacteria (어성초(Houttuynia cordata Thunb) 유산균 발효물의 항산화 활성)

  • Kim, Yong-Min;Jeong, Hae-Jin;Chung, Hun-Sik;Seong, Jong-Hwan;Kim, Han-Soo;Kim, Dong-Seob;Lee, Young-Guen
    • Journal of Life Science
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    • v.26 no.4
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    • pp.468-474
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    • 2016
  • This study was performed to evaluate the possibility of application of lactic acid bacteria fermentation to increase the anti-oxidative activity of extracts from Houttuynia cordata Thunb. Houttuynia cordata Thunb. was fermented by two species of lactic acid bacteria, Leuconostoc mesenteroides 4395 and Lactobacillus sakei 383. The anti-oxidative activities of Houttuynia cordata Thunb. extracts were analyzed both before and after fermentation. Anti-oxidative activity was determined by in vitro assays to measure 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and super oxide dismutase (SOD)-like activities, and by determining total flavonoid and total phenolic compound contents. The extracts of fermented Houttuynia cordata Thunb. had higher anti-oxidative activity than the unfermented control. The DPPH scavenging activity of the extracts after fermentation by Leuconostoc mesenteroides 4395 at 30℃ for 5 days was 71.67±0.52%, and after Lactobacillus sakei 383 fermentation at 35℃ for 5 days was 70.11±0.67%; these activities were both about 20% higher than the control. Increases of about 10 mg GAE/g of total phenolic compounds were found in both fermented extracts and both contained about 6 mg quercetin equivalents/g of total flavonoids, compared with 35.90±0.61 mg/g and 21.69±1.52 mg/g in the control, respectively. These results also suggested that fermentation time and temperature were important factors in determining the anti-oxidative effect of extracts from fermented Houttuynia cordata Thunb. These findings should be valuable for the development of medicines or functional foods with antioxidative activity.

Effect of Nitrogen Application Rate on Growth and Yields of Aster scaber Thunb. and Ligularia fischeri Turcz. in the First Year after Transplanting (참취와 곰취의 정식 후 1년차 수량 및 생육특성에 미치는 질소 시용 효과)

  • Choi, Seong-Chul;Ahn, Mun-Sup;Ahn, Su-Yeong;Ok, Yong-Sik;Son, Jung-Su;Joo, Jin-Ho
    • Korean Journal of Environmental Agriculture
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    • v.28 no.3
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    • pp.243-248
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    • 2009
  • Chinese cabbage cultivation has been typically practiced in alpine sloped farmland in Gangwon province, and caused severe soil erosion by soil disturbance like tillage. Cultivation of wild edible greens such as Aster scaber Thunb. and Ligularia fischeri Turcz. could be one of alternative ways to reduce soil erosion, because their residues become stubble mulch after harvest, and there is no need for tillage every year. Therefore, the objective of this research was to estimate the effect of nitrogen application rates for the maximum production of Aster scaber Thunb. and Ligularia fischeri Turcz. in the early cultivation stages. Total N contents of Aster scaber Thunb. and Ligularia fischeri Turcz. increased with the increment of fertilizer application rates. Maximum growth of Aster scaber Thunb. and Ligularia fischeri Turcz. was observed with the highest rates of fertilizer. We obtained the relationship between Aster scaber Thunb. and N application rate, as y = $-0.296x^2$ + 73.82x + 5246 (R = 0.998). There was no significant difference for Aster scaber Thunb production between N application rates for 120 and 160 kg/ha. Also, the relationship between Ligularia fischeri Turcz. and N application rate was y = $-0.135x^2$ + 32.86x + 1529 ($R^2$ = 0.965) in the first year trial after transplanting.

Quality Characteristics of Jeungpyun Prepared with Different Ratios of polygonum multiflorum Thunb Powder (하수오 분말을 첨가한 증편의 품질특성)

  • Lee, Gae-Soon;Park, Geum-Soon
    • Korean journal of food and cookery science
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    • v.27 no.4
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    • pp.35-46
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    • 2011
  • We developed hasuo jeungpyun with added polygonum multiflorum Thunb powder to increase the functional value and flavor of traditional jeungpyun. Hasuo jeungpyun containing 0, 2, 4, 6 and 8% polygonum multiflorum Thunb powder was prepared and the moisture contents, pH, color, texture and sensory analysis of the samples were measured. With increasing polygonum multiflorum Thunb powder content, the moisture content of hasuo jeungpyun decreased, and pH increased significantly. Hasuo jeungpyun prepared with 2-4% polygonum multiflorum Thunb powder displayed significantly higher swelling power. As the level of polygonum multiflorum Thunb powder increased, the L-value decreased, whereas the a- and, b-values increased. A textural profile analysis showed that the hardness increased but cohesiveness decreased with increasing polygonum multiflorum Thunb powder content. A sensory evaluation showed the highest value for air cell uniformity, flavor, and overall acceptability of 4% hasuo jeungpyun, which suggests hasuo jeungpyun with improved sensory characteristics. In the analysis of the correlation between sensory characteristics and mechanical properties of hasuo jeungpyun, hardness and brittleness were negatively correlated with after swallow, appearance, flavor, texture, taste, and overall acceptability.

The Lipidperoxidative effect of Houttuynia cordata Thunb & Saururus chinensis

  • Jung , Hae-Jin;Park, Sun-Yi;Ha , Bae-Jin
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.322.2-322.2
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    • 2002
  • Houttuynia cordata Thunb is a traditional medicine which has been used as antidote and antiphlogistic agent. Saururus chinensis is a perennial herb which cultivated as medicinal and decorative use. the aerial part of which have been used for the treatment of edema, jaundice and gonorrhoea in Korean folk medicine. (omitted)

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Studies on The Free Vibrational Properties of Traditional and Replaceable Species for Sounding Board (향판용(響板用) 관행수종(慣行樹種)과 대체가능수종(代替可能樹種)의 자유진동적(自由振動的) 성질(性質)에 관한 연구(硏究))

  • Kang, Wook;Jung, Hee-Suk
    • Journal of the Korean Wood Science and Technology
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    • v.16 no.3
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    • pp.48-64
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    • 1988
  • This study was carried out to investigate the free vibrational properties of traditional and replaceable species for sounding board, using piezoelectrical accelerometer and signal analyzer. In the study, the frequency equations of free-free beam carrying a concentrated mass in the transverse vibration and of free-mass beam in the longitudinal vibration were used. The results obtained were as follows. 1. Average values of dynamic modulus of rigidity of Korean commercial species measured were as follows. Paulownia tomatosa (Thunb.) Steudel: $5.590\times10^9\;dyne/cm^2$ Tilia amurensis Rupr.: $5.414\times10^9\;dyne/cm^2$ Macckia amurensis Rupr.: $10.044\times10^9\;dyne/cm^2$ Fraxinus mandshurica Rupr.: $8.876\times10^9\;dyne/cm^2$ Ulmus davidiana var.japonica Nakai: $8.677\times10^9\;dyne/cm^2$ Pinus rigida Miller: $6.33\times10^9\;dyne/cm^2$ Fraxinus rhynchophylla Hance: $4.666\times10^9\;dyne/cm^2$ 2. The ratio of dynamic transverse Young's modulus to dynamic modulus of rigidity, $E_T/G_{LT}$, was 24.922 for Fraxinus rhynchophylla Hance, which was the highest, 7.317 for Paulownia tomentosa (Thunb.) Steudel, which was the lowest among Korean commercial species measured. 3. The value of longitudinal dynamic Young's modulus was about 3.7% and 19.8%, respectively, higher than those of transverse dynamic and static Young's modulus. The value of transverse dynamic Young's modulus was about 15.5% higher than that of static Young's modulus. 4. Predicted value of MOR in terms of correlation coefficient by transverse dynamic Young's modulus was slightly higher than that of static Young's modulus, but no significance was found out. 5. Transverse dynamic Youne's modulus was $(2.002\pm0.288)\times10^{11}\;dyne/cm^2$ for Fraxinus mandshurlca Rupr., which was the highest, $(0.601\pm0.100)\times10^{11}\;dyne/cm^2$ for Paulownia tomentosa (Thunb.) Steudel, which was the lowest among Korean commercial species measured. The sound velocity of sitka spruce was 5,685 m/sec, which was the highest. 6. Internal friction of Paulownia tomentosa (Thunb.) Steudel was shown the lowest value among Korean commercial species, $(8.795\pm1.795)\times10^{-3}$, but was higher than that of sitka spruce, $(7.331\pm0.991)\times10^{-3}$. Internal friction was shown negative correlation with density and dynamic Young's modulus, respectively. 7. K value was affected largely by internal friction and was the highest, $2.225\times10^8$ for Paulownia tomentosa (Thunb.) Steudel and was the lowest, $0.550\times10^8$ for Fraxinus rhynchophylla Hance. K value of Paulownia tomentosa (Thunb.) Steudel was higher than that of sitka spruce and K values of melapi and cottonwood, which have been considered to be replaceable species with sitka spruce in the piano industry, were lower than those of Paulownia tomentosa (Thunb.) Steudel and mill amurensis Rupr.

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Anti-allergic Activity of the Extracts from Houttuynia cordata Thunb Fermented by Lactic Acid Bacteria (어성초(Houttuynia cordata Thunb) 유산균 발효물의 항알러지 활성)

  • Lee, Young-Guen;Kim, Yong-Min;Jeong, Hae-Jin;Lee, Bo kyung;Lee, Sung-Eun;Im, Dong-Soon;Kim, Han-Soo
    • Journal of Environmental Science International
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    • v.26 no.12
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    • pp.1355-1362
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    • 2017
  • This study was performed to evaluate the possibility of application of lactic acid bacteria fermentation to increase the anti-allergic activity of the extracts from Houttuynia cordata Thunb. H. cordata Thunb was fermented on 25, 30, 35 and $40^{\circ}C$ for 5 days by two species of lactic acid bacteria, Leuconostoc mesenteroides 4395 and Lactobacillus sakei 383. The anti-allergic activity of water extracts of H. cordata Thunb was then analyzed both before and after fermentation. Anti-allergic activity was determined in vitro assays by using 5-lipoxygenase (5-LO), cyclooxygenase-2 (COX-2) and ${\beta}$-hexoseaminidase release of RBL-2H3 cells (degranulation marker). The extracts fermented at $35^{\circ}C$ by both bacteria had the highest inhibitory activities against 5-LO, and also higher than the control, and the one fermented at $30^{\circ}C$ by both bacteria had the highest inhibitory activity against COX-2. The degranulation of RBL-2H3 cells induced by IgE-antigen complex was estimated as ${\beta}$-hexoseaminidase release rate as reference of 100%, the release rates were inhibited in $25{\mu}g/ml$ of the extracts fermented at 30, 35 and $40^{\circ}C$ only by L. mesenteroides 4395. These results indicate that anti-allergic activity of H. cordata Thunb is increased by lactic acid bacteria fermentation.

Antioxidation and Anti-inflammation Activity of Isodon inflexus (Thunb.) Kudo Extract and Its Isolated Substance (산박하 추출물과 분리물질의 항산화 및 항염증 활성)

  • Bu, Hee-Jung;Lee, Sunjoo
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.42 no.3
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    • pp.257-268
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    • 2016
  • The functions of anti-oxidation and anti-inflammation were investigated with the crude 80% methanol extract, subfractions and henryin isolated from Isodon inflexus (Thunb.) Kudo (I. inflexus (Thunb.) Kudo). Antioxidative ability was evaluated by bioassays using 2, 2-diphenyl-1-1-picrydrazyl (DPPH) free radical scavenging activity, xanthine oxidase inhibition, and superoxide radical scavenging effects. Ethyl acetate and butanol fractions exhibited free radical scavenging activity on superoxide with $IC_{50}$ values of $0.9{\mu}g/mL$, $0.2{\mu}g/mL$, respectively, which were stronger activity than that of allopurinol ($2.2{\mu}g/mL$) as reference. For the inhibition of anti-inflammatory activity in RAW 264.7 cell, the ethyl acetate fraction showed strong inhibition activity NO production, and henryin isolated from its subfraction reduced the activity in a dose-dependent manner. Ethyl acetate fraction and henryin suppressed not only mRNA expression of iNOS and COX-2, but also the mRNA expression of pre-inflammatory cytokines such as, TNF-${\alpha}$, 1L-$1{\beta}$, IL-6, in a dose-dependent manner. These results suggested that ethyl acetate fraction of I. inflexus (Thunb.) Kudo has considerable potential as a cosmetics ingredient with an antioxidant and anti-inflammatory effects and henryin can be applied as an functional reference.