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http://dx.doi.org/10.15230/SCSK.2016.42.3.257

Antioxidation and Anti-inflammation Activity of Isodon inflexus (Thunb.) Kudo Extract and Its Isolated Substance  

Bu, Hee-Jung (Biotechnology Regional Innovation Center, Jeju National University)
Lee, Sunjoo (Faculty of Chemistry & Cosmetics, College of Natural Sciences, and Cosmetic Science Research Center, Jeju National University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.42, no.3, 2016 , pp. 257-268 More about this Journal
Abstract
The functions of anti-oxidation and anti-inflammation were investigated with the crude 80% methanol extract, subfractions and henryin isolated from Isodon inflexus (Thunb.) Kudo (I. inflexus (Thunb.) Kudo). Antioxidative ability was evaluated by bioassays using 2, 2-diphenyl-1-1-picrydrazyl (DPPH) free radical scavenging activity, xanthine oxidase inhibition, and superoxide radical scavenging effects. Ethyl acetate and butanol fractions exhibited free radical scavenging activity on superoxide with $IC_{50}$ values of $0.9{\mu}g/mL$, $0.2{\mu}g/mL$, respectively, which were stronger activity than that of allopurinol ($2.2{\mu}g/mL$) as reference. For the inhibition of anti-inflammatory activity in RAW 264.7 cell, the ethyl acetate fraction showed strong inhibition activity NO production, and henryin isolated from its subfraction reduced the activity in a dose-dependent manner. Ethyl acetate fraction and henryin suppressed not only mRNA expression of iNOS and COX-2, but also the mRNA expression of pre-inflammatory cytokines such as, TNF-${\alpha}$, 1L-$1{\beta}$, IL-6, in a dose-dependent manner. These results suggested that ethyl acetate fraction of I. inflexus (Thunb.) Kudo has considerable potential as a cosmetics ingredient with an antioxidant and anti-inflammatory effects and henryin can be applied as an functional reference.
Keywords
antioxidant; anti-imflammation; henryin; I. inflexus (Thunb.) Kudo; cosmetics;
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