• The Plant Pathology Journal
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• v.22 no.2
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• pp.139-146
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• 2006

The 3' region of Potato virus X (PVX) has the 74 nt 3'-nontranslated region (NTR) that is conserved among all potexviruses and contains several cis-acting elements for minus-strand and plus-strand RNA accumulation. Three stem-loop structures (SL1-SL3), especially formation of SL3 and U-rich sequence of SL2, and near upstream elements in the 3' NTR were previously demonstrated as important cis-acting elements. To Investigate the binding of these cis-acting elements within 3' end with host protein, we used the electrophoretic mobility shift assays (EMSA) and UV-cross linking analysis. The EMSA with cellular extracts from tobacco and RNA transcripts corresponding to the 150 nt of the 3' end of PVX RNA showed that the 3' end of PVX formed complexes with cellular proteins. The specificity of protein binding was confirmed through competition assay by using with 50-fold excess of specific and non-specific probes. We also conducted EMSA with RNAs containing various mutants on those cis-acting elements (${\Delta}10$10, SL3B, SL2A and ${\Delta}21$; J Mol Biol 326, 701-720) required for efficient PVX RNA accumulation. These analyses supported that these cis-acting elements are required for interaction with host protein(s). UV-cross linking analysis revealed that at least three major host proteins of about 28, 32, and 42 kDa in mass bound to these cis-elements. These results indicate that cis-acting elements from 3' end which are important for minus and plus-strand RNA accumulation are also required for host protein binding.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.34 no.4
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• pp.40-46
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• 2006

It is required to develop a highly reliable attitude & orbit control system of satellite that is less expensive as the technology of satellite design & integration is recently matured dramatically. To accomodate this kind of needs, the two axis attitude control method for wheel-based momentum-biased satellite system whose momentum bias vector points to a certain direction(sun direction), is developed using simple but reliable sensors and actuator: three axis magnetometer and coarse sun sensor are used as sensors, and magnetic torque bars are used as actuator. Classical PD type controller design methodologies are applied on a satellite system for the two axis control with the proper assumptions. Nonlinear simulation results are included to demonstrate the long term stability and the performance of closed-loop system design results.

• The Research Journal of the Costume Culture
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• v.18 no.1
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• pp.109-117
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• 2010

Knit fabrics are created on diverse machines with diverse knit loops and conditions to make different patterns and fabric types. Dimensional modifications of knit fabrics can also be achieved by numerous methods such as different knit-loop structures, different types of yarns, or different finishing processes including heat setting, steaming, chemical treatment et cetera. This research develops and explores sophisticated three-dimensional knit fabrics by combining the several different knit stitches including rib and purl. This study focuses on 3D knit models created on modern electronic weft (flat V-bed) knitting machines which have capability of individual needle selection. Several samples of the 3D knitted fabrics are also examined in this research. This research furthermore suggests new types of knitted fashion garment made by using the interesting physical effects.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2005.11a
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• pp.387-390
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• 2005

A This paper presents a novel three-phase current-fed Pulse Width Modulation converter with switched- capacitor type resonant DC link commutation circuit operating PWM pattern strategy under a design consideration of low-pass filter, which can operate on the basis of the principle of zero current soft-switching commutation. In the first place, the steady-state operating principle of this converter with a new resonant DC link snubber circuit is described in connection with the equivalent operation circuit, together with the practical design procedure of the switched-capacitor type resonant DC link circuit is discussed from a theoretical viewpoint on the basis of a design example for high-power applications. The actively delayed time correction method to compensate distorted currents due to a relatively long resonant commutation time is newly implemented in the open loop control scheme so as to acquire the new optimum PWM pattern. Finally, the experiment or set-up in laboratory system or this converter is concretely demonstrated herein to confirm a zero current soft-switching commutation of this converter. The comparative evaluations between current-fed hard switching PWM and soft-switching PWM converters are carried out from a viewpoint of their PWM converter characteristics.

• The Transactions of the Korean Institute of Power Electronics
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• v.16 no.4
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• pp.309-316
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• 2011

A PLL system has widely used for synchronizing the grid voltage at the grid-connected inverter for supplying power from the PV generation systems. In this paper, a PLL algorithm without both the loop filter and PI controller is suggested for improving the performance of synchronization at the single-phase and three-phase grid connected inverters. In order that the output voltage of a phase detector in the PLL has only a dc voltage, and it approaches to 0 when the synchronization signal is locked to the grid voltage, the feedback signals are determined by using two-phase voltages. After the PLL system with a proportional controller is modelled with the small signal analysis, the stability and steady-state error are investigated. Through the simulation studies and experimental results, the performances of the proposed PLL algorithm are verified.

• Journal of Power Electronics
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• v.18 no.1
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• pp.234-250
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• 2018

This paper presents a new load sharing control for use between paralleled three-phase inverters in an islanded microgrid based on the online line impedance estimation by the use of a Kalman filter. In this study, the mismatch of power sharing when the line impedance changes due to temperature, frequency, significant differences in line parameters and the requirements of the Plug-and-Play mode for inverters connected to a microgrid has been solved. In addition, this paper also presents a new droop control method working with the line impedance that is different from the traditional droop algorithm when the line impedance is assumed to be pure resistance or pure inductance. In this paper, the line impedance estimation for parallel inverters uses the minimum square method combined with a Kalman filter. In addition, the secondary control loops are designed to restore the voltage amplitude and frequency of a microgrid by using a combined nominal value SOGI-PLL with a generalized integral block and phase lock loop to monitor the exact voltage magnitude and frequency phase at the PCC. A control model has been simulated in Matlab/Simulink with three voltage source inverters connected in parallel for different ratios of power sharing. The simulation results demonstrate the accuracy of the proposed control method.

• Nuclear Engineering and Technology
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• v.51 no.2
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• pp.356-368
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• 2019

This paper presents the verification and validation (V&V) of the STREAM/RAST-K 2.0 code system for a pressurized water reactor (PWR) analysis. A lattice physics code STREAM and a nodal diffusion code RAST-K 2.0 have been developed by a computational reactor physics and experiment laboratory (CORE) of Ulsan National Institute of Science and Technology (UNIST) for an accurate two-step PWR analysis. The calculation modules of each code were already verified against various benchmark problems, whereas this paper focuses on the V&V of linked code system. Three PWR type reactor cores, OPR-1000, three-loop Westinghouse reactor core, and APR-1400, are selected as V&V target plants. This code system, for verification, is compared against the conventional code systems used for the calculations in nuclear design reports (NDRs) and validated against measured plant data. Compared parameters are as follows: critical boron concentration (CBC), axial shape index (ASI), assembly-wise power distribution, burnup distribution and peaking factors. STREAM/RAST-K 2.0 shows the RMS error of critical boron concentration within 20 ppm, and the RMS error of assembly power within 1.34% for all the cycles of all reactors.

• Journal of Fashion Business
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• v.23 no.2
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• pp.1-17
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• 2019

The objective of this study was to investigate electrical conductivity of fabrics from polyester (PET) and Nylon (N) containing polyurethane (PU), with silver paste patterns screen-stenciled in three directions. The PET/PU and N/PU fabrics knitted or woven were uniaxially strain-recovered up to 22.5% in three times when each change in electrical resistance was simultaneously measured. This study established four variables that complexly affected electrical conductivity of these specimens; fabric structures, components, cover factors, and the percolation of silver particles. The woven or knitted fabric structures did not distinctively cause the changes in electrical resistance, however, the woven fabrics with the diagonal patterns showed their relatively high electrical resistance. The PET/PU fabrics with increasing the PET proportion generally presented the opposite propensity to its electrical conductivity. The changes in electric resistance of the PET/PU 85/15 2/1 twill and double plain fabrics instantaneously responded to the rate of elongation. The PET/PU group exhibited a reverse correlation between its cover factor and electrical resistivity. The highest electrical conductivity of the PET/PU 95/5 interlock fabric, with very few fluctuations, was attributed to the deep percolation of the silver particles that bridged the gaps between one loop and another. On the other hand, the occurrence of the silver cracks along with the elongated direction led to the immeasurably high change in electrical resistance as the strain increased.

• Journal of the Korean Society of Systems Engineering
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• v.18 no.2
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• pp.75-93
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• 2022

Machine learning (ML) data-driven meta-model is proposed as a surrogate model to reduce the excessive computational cost of the physics-based model and facilitate the real-time prediction of a nuclear power plant's transient response. To forecast the transient response three machine learning (ML) meta-models based on recurrent neural networks (RNNs); specifically, Long Short Term Memory (LSTM), Gated Recurrent Unit (GRU), and a sequence combination of Convolutional Neural Network (CNN) and LSTM are developed. The chosen accident scenario is a control element assembly withdrawal at power concurrent with the Loss Of Offsite Power (LOOP). The transient response was obtained using the best estimate thermal hydraulics code, MARS-KS, and cross-validated against the Design and control document (DCD). DAKOTA software is loosely coupled with MARS-KS code via a python interface to perform the Best Estimate Plus Uncertainty Quantification (BEPU) analysis and generate a time series database of the system response to train, test and validate the ML meta-models. Key uncertain parameters identified as required by the CASU methodology were propagated using the non-parametric Monte-Carlo (MC) random propagation and Latin Hypercube Sampling technique until a statistically significant database (181 samples) as required by Wilk's fifth order is achieved with 95% probability and 95% confidence level. The three ML RNN models were built and optimized with the help of the Talos tool and demonstrated excellent performance in forecasting the most probable NPP transient response. This research was guided by the Systems Engineering (SE) approach for the systematic and efficient planning and execution of the research.

• Journal of the Korean Chemical Society
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• v.67 no.2
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• pp.81-88
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• 2023

The contribution of hydrophobic residues to the protein folding reaction was studied by using HubWA variant proteins with I and L to V mutation. Folding kinetics of all V variant proteins was observed to be satisfied by a three-state on-pathway mechanism, U ⇌ I ⇌ N, where U, I, and N represent unfolded, intermediate, and native state, respectively. Three-state folding reaction was quantitatively analyzed and the free energy of folding of each elementary reactions and overall folding reaction, ΔG^o_UI, ΔG^o_IN, and ΔG^o_UN, were obtained. From the ratio of free energy difference between the variant protein and HubWA, ΔΔG^o_UI/ΔΔG^o_UN (ΔΔG^o_UI = ΔG^o_UI (variant protein) - ΔG^o_UI (HubWA) and ΔΔG^o_UN = ΔG^o_UN (variant protein) - ΔG^o_UN(HubWA)), the contribution of hydrophobic residues to HubWA folding was analyzed. The residues which are located in the hydrophobic core between α-helix and β-sheet, I3, I13, L15, I30, L43, I61 and L67, showed ΔΔG^o_UI/ΔΔG^o_UN value of ~0.5 when each of these residues was mutated to V, indicating that these residues form relatively solid hydrophobic core in the intermediate state. Residues located at the end of secondary structures and loop, I23, L69 and I36 showed ΔΔG^o_UI/ΔΔG^o_UN value below 0.4 when each of these residues was mutated to V, indicating that the region containing these residues are loosely formed in the intermediate state. V17A, L50V and L56V showed fairly high ΔΔG^o_UI/ΔΔG^o_UN value of ~0.8. Since L50 and L56 are located in the region containing long loop (residue 46 to 62), it is suggested that the high ΔΔG^o_UI/ΔΔG^o_UN value of these residues prevents the formation of aggregate at the early stage of folding reaction.